Day 37. Girls are flowering !!!! Yesterday I gave them heavy feed . Checked ppm only at the end - 1550 ;))))) thought they will burn, leaves went curly during day and I had to spray them with BioHeaven really often .. Night photo shows all good so , I will give bacterial feed next watering and will do one more feed bomb. Will target my favourite 1200, but you never know ... ;))) Hopefully tomorrow I will dump last Northern Lights and will rearrange all tent. Second wave of FB needs bigger pots too ... It's amazing what they did under that little light, but I think WeedSeedExpress grow proved - all Mars Hydro SP light line - pure joy for grower !!! SP150 will go to grow wonderful plants in Wooster very soon ... One more grower will be born ! ;))) Busy busy busy ;))) Day 40. Two girls got supercrop, they stretched too much. Both got bottom clearing too, other girls still left for a week, till any training will be done . After 6 hours girls are looking up already and all is good , just overcrowded ;)))) Happy Growing !!!

August 12 - Day 64 - Watered with 3L of water, BioGrow, BioBloom, and TopMax.

DAY 14 F: Hi all. I hope we are all coping and navigating this tumultuous time in the "other world" . It has been a really good week for the ladies this week and even the little chica has improved slightly from her attempted poisoning by hands yet unknown ????. Lol They have all been really productive in their stretch periods and i had to move the fc3000 up a fair bit to stop them reaching the panel. I want to avoid any supercropping to use the plants natural structures on this final auto run. The tip spreading has been effective at stopping them over growing the height restriction and has now used the stretch period to space all the budsites into good positions for light and air movement. I want to also avoid having to remove any of the powerhouse fan leaves that are going to help convert and store all those sugars and goodies for the bud development soon. Inwill tet to keep them from light blocking lower leaves and growth by tucking them away where I can. They are around 2ft in height with the Strawberry Pie being the taller of the 3 strains. All have fairly long node gaps whichb had hoped to avoid so they would stack the buds rather than grow in small clusters but I know from previously growing them all that they make some very nice harvested and cured nugs. I am still only feeding water but will be doing another tea of biosys and a top dressing of life-cycle too this week to give the biosphere a hand and the girls a boost from that. The light is really doing them justice with plenty of deep penetration to the soil bed in places too. There are no signs of hot spots or too much intensity so far so an even spread of those light ranges has fed them perfectly. With the convenience of the soil beds , In the tent I have from Mars being the perfect size for this light , it could be a match made in heaven for the upcoming photoperiod run with the FC3000 too. Just need to find a way to hang the tent with my horrible apex roof first..... lol With Bud Porn on order for next weeks update , I bid you all farewell or as we say in the UK, "tatty bye for now" growmies. Be lucky

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Starting to keep a close eye on the Trichomes. Still clear some cloudy. Stopped feeding, Starting to flush. It’s starting to smell amazing in here.

this week has just started but im anticipating serious growth with my tent and mars led when it arrives :D still need to buy proper nutes and a ph tester but update ( just wait till you see how much the mars ts 1000 has helped her

Que pasa familia, vamos con la primera semana de floración de estas Tropicana poison F1 de Sweetseeds. Vamos al lío , las 3 plantas seleccionadas fueron trasplantadas a su maceta definitiva, ya superaron el shock por el trasplante, estas semanas las paremos todas a floración. El ph se controla en 6.2 , la temperatura la tenemos entre 20/22 grados y la humedad ronda el 50%. El ciclo de florscion 12h de luz, el foco está al 100% de potencia. Hasta aquí todo, Buenos humos 💨💨💨

She is going to be flipped to 12/12 next week she is so ready for 12/12 🙏💪💚💜💚💪🤣💪💚💜

DAY 35 Flowering: A good week for bud growth this week and plenty of light to reach all those tucked away bud sets too. The room looks so full now with the monster sized Strawberry Pie dominating the canopy and pushing the height limits. All of the buds on these two girls , including the secondaries, are a good size with so much vigerous pistil growth going on now that they look like their own plants. These girls need a lot of room to benefit from their natural size proportions and this was AFTER I used tip spreading to keep them lower !!!. Not sure the plant took me serious now !!. Lol I could not be more impressed with these at this point and I can see 2 potential auto personal bests forming.... The Six Shooter #1 is a nice sized lady too. Inhave had to do a little bending and rescuing on a couple of branches as they were too far out of light and also blocking other growth but she looks good on node spacing too so should have some nicely stacked colas at harvest time. Her sister #2 is half her size but has a lot of side growth to compensate thankfully. Being so low down compared to the other 5 plants has thankfully not been too much if an issue thanks to the FC3000 being so penetrative. Even the buds at the very back of the grow ate getting ample light to really bang the weight on from feeding and converting. Both of the six shooter are starting to colour up with orange hairs now and it does look like they could be the 1st to finish at this point. Mexican Airline are developing steadily. The #1 is rmteying toncatch upnwith bud production now but her long node lengths suggest she will be more clumps of flowers than stacks , her yield will no doubt be far lower than her sisters due to her slow start and progress but she still looks like she could pull through with some decent nugs. #2 has a lot shorter nodes and should look a little more spikes hopefully. They are showing one or two amber hairs now at just past the halfway point so we are doing ok. Lol All in all , I am loving this run of plants and the light has performed so well considering the canopy difference. Until next time. Be well Growmies.

Big shout to RQS and @James for the beans for this grow. Once again the genetics from these guys is unbelievable. Really appreciate all the likes messages and support from you awesome bunch of growers. ✌️🙌💚 Week 9 is upon us. Loving the way this Mimosa is filling out. Really putting some weight on. The pics aren’t doing much justice. Will carry on feeding for another 2 weeks the start the flush. The colours are really starting to come through. I will monitor the progression closely now and let the plants tell me what they need. Have a great week people ✌️👊🏻

Buenos días familia, hoy traemos la 3 semana de floración de nuestras crazy cookies, zambezaseeds. Es una cepa bastante vigorosa con un tallo robusto 6mm, exactamente a mitad altura. Su cultivo es fácil, apenas se ve bloqueo alguno, traga muy bien en lo que se refiere a nutrientes. Ph en 6,5 y humedad por debajo de los 45 % . 48h entre riego y riego, no olvidemos que están en tiestos de 7L. Su floración está siendo bastante rápida estas semanas se verá como engordan nuestras flores, sin duda este es el momento para meter caña a big bud y bud candy.

Fun week- buds started packing on mass and frost. just watering til chop day, except for the green crack which is about halfway through flower.

They are looking strong and healthy with decent branching before LST. I expect them to be some nice little bushes. I am thoroughly impressed at the rate of growth and maturing on these. I really hope they don't finish in 7 weeks. They are already clearly into their stretch and beginning to make bud sites on day 26.

Start of week 3 everything looking good. On day 16 I transplanted them to fabric pots. Except for the absolute unit in the middle. I left that one alone as it seems to have overtaken everything else. Using RO water and I add tiny amount of tap water to bring PH up to around 6.2. 3Liters RO and 250ml of my tap turned it into 6.2 ish PH from 5.8

Hello guys. My plants are flowering well, but I thought I was almost ready to harvest but it seems it's still growing.. How much do you think I'll get from the taison ? And for the fat banana ? I have no idea of the quantity I'll get so I'm curious to know your guess ! 🌾

Se me funde la lámpara en pleno horario de luz 😅 Bueno, ha durado bastantes años. Con el humidificador y la nueva deberían acabar a full la floración. La he tenido unas 15 horas con una bombilla hasta que me llegara la lámpara nueva. Parece que ya no habrá mas inconvenientes hasta el final. Lets Go!