July 11: Finally this week getting some heat with 25-30 deg C daytime highs. Plants are thriving with a lot of vertical growth and flowers starting to build. Resin is developing nicely. CBD Crack is a smaller plant comparatively, but the resin smells spectacular.

Patiently waiting for these girls to begin their final descent! Gonna be some monster plants! Momster buds!!! Steady on my nutrient schedule this week and she made some big gains!!!

Don’t judge. A few days away from chop! Again... looking forward to see how the “stress” improves quality...? Photos/video taken 133 days after breaking soil, day 56 of flower. UPDATE: Plant harvested on 1/7; day 136 after breaking soil, day 59 of flower.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

This week is marked by high temperatures and humidity because grow room is under roof :/ But still I do my best. Branches bending over because buds are too big and heavy, last flushing week ahead!

Day 56 update, and man, the summer heatwave is officially pushing us to the limit! Inside the greenhouse, it’s getting incredibly hot, but the Gorilla Melon is handling it like an absolute champ. No real signs of heat stress yet, though you can tell she is working hard. Honestly, the AutoPot system is the real MVP this week; with these soaring temperatures, the constant water supply from below keeps her perfectly hydrated without any scary dry spells. To keep the canopy nice and even, I went ahead and did some more topping on the taller shoots to balance out the height before she gets any bigger. On the pest front, we had a little drama: the ChrysoCards unfortunately baked in the intense heat and didn't survive. Lesson learned! To fix this, I released a fresh batch of live green lacewing larvae instead. I just put them right onto the main branch junctions on the plant. It’s working beautifully—the thrips are visibly retreating, and I’ve barely seen any new damage on the big fan leaves this week. Slowly but surely, we are winning this battle!

The plants continued to grow vigorously, so I decided to start flowering at the end of this veg week. They been very healthy! Krazy Runtz - Veg Time; 63 Days since planted. Wedding Cake & Strawberry Cough - Veg Time; 67 Days since planted. Pringle Grow (Strawberry Cough) - Veg Time; 52-ish Days since planted.

Another week down and I think its time to move the camera up a bit. Getting hard to keep them both in that dumpy camera's optics. At some point I'm doing to invest in some decent glass for these videos. The plants seem to be rocking along fine after their initial topping. Did some light defoliating here and there but I'll pretty much leave them alone from this point on. I might do a bit more training but honestly I'm not after a perfect canopy.

Lil youngin on their way home should be home for 4/20 along with 2 of their mother...as for the breeding project pollination is a success also been harvesting pollen(feminized) for later use Mothers are growing well jus to continue cut and set aiming for about 1000 already cut 3 and got 100...happy growing

Day 15 / 15.04. For the start of week 3 of Veg I decided to transplant the plants into their final 15l autopots. Because I only have space for 3 plants I threw out the runty Super Lemon Haze plant. I transplanted all of them into BioBizz Biomix and sprinkeled mycorrhizae on the roots. I watered them in with 300ml of nutrient solution at 6.5 Ph and 1.1 mS/cm TDS. The VPD has now reached 0.8 kPa. With the transplant I also switched the small light the plants were under to the Mars Hydro TS-3000 on 20% brightness. This means plants' DLI has been bumped up to about 20 mol/m2/d. Day 17 / 17.04. The big light has blown the third relay of the smart system in a row. This means lights turned on about half a hour late today. Day 19 / 19.04. Today I topped all of the plants at the fourth node. I initially planned to top on day 21 but they did not experience any shock from transplant so I got to do it today. Day 21 / 21.04. For the end of week 3 veg I filled up the autopot reservoir and turned it on. I'm expecting some even greater growth now.

Hello everyone, The 3 weird mystery plants are doing their thing and are still under 24 hours of light per day. One of the 3 did receive 15ml of pk13/14 per 10 liter water the other 2 only get 3 ml per 10 liter water. So i checked the expert seeds catalogus and don't see Afghan skunk auto seeds only feminized seeds but my 3 plants are clearly automatic flowering. Let's see how they look next week. Best regards, Growfather

Ufff... Fortunately finish... 😄 Starting a new Breedbros project, see ya soon

==> Decreased the base nutrients for less Nitrogen She is doing great! Calyxes swelling nicely :) Starting to get some color in the leaves on the higher buds :D The nice smells of watermelon candy and sour weed are slowly intensifying and the macro pictures surprised me! I can't wait till she is ready, I think she will be ready first :D

Hello my friends 👨‍🌾👩‍🌾, This week was good, I've continue to train the plants, I've removed some leaf 🍃, Plant healthy with a sweet green and growing well 😊. The Vanilla and the Bisous Au Thc, have a similar size, looking good for the scrog, after the fat banana harvest. 💦 I give water each 2 or 3 days 350 ml/plant Water + rhizo + tera vega Water + Rhizo + canazym PH@6 Lamp is 100% Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Merry Christmas 🎅 🎄 and happy nice end year, See you next week Mars Hydro - TS 1000 https://www.mars-hydro.com/ts-1000-led-grow-light Mars Hydro - FC3000 https://www.mars-hydro.com/fc-3000-samsung-lm301b-led-grow-light Mars Hydro - SP3000 https://www.mars-hydro.com/sp-3000-samsung-lm301b-greenhouse-led-grow-light The High Chameleon - Vannila T https://www.thehighchameleon.com/shop/vanilla-t-5

Well I said I was leaving her untrained but on day 56 I gently tied back a few branches to open up the canopy. This is looking like it's going to be my best grow yet.

COntinued to apply small amounts of pressure with LST but aside from that, letting her do her thing. Had to leave her for a few days and she got dehydrated, after a feed she perked up again but she's now started sucking all the nutes out of her lower growth. She has a lot so I'm hoping it doesn't affect the harvest. Would have preferred this to begin with my flush. For more content on all my grows please check out my Instagram! Link is on my profile!

Going well

Week 6 Veg - January 16/21 to 22/21 ***** Week 7 will be their last week being able to take them out of the tent😟 SCROG netting going in on the weekend ahead. Not really a lot to report this week as the girls are doing well and I am just wanting their side branches to grow longer to help filling in the canopy anyway. Performed more LST this week and bending over the branches daily. Didn’t do any HST this week, will once the net is in and I have a better visual of how the branches need to bend. They have come a long ways over the last 2 weeks when you look back at pictures. They have filled out with more side branching and other than battling a little purple striping on the main stems they seem pretty happy. Leaf colour is good and should be in good shape for the flip soon. Will go through all of week 8 before flipping so hopefully week 9 will be transition week and week 10 the start of flower.......have to get through next week first though😀👍 Switching over to tap water again. I have been struggling with calcium and magnesium deficiencies on all grows for the last year. I can’t seem to get the right amount added back to the RO water I use in all feedings. So running with straight water again now. Ppm coming out is 325 so I will work with that. Going to explore my own RO system in the house rather than bottled water, a system that adds back calcium, magnesium, and iron. It’s been flowing okay for the first week and will continue on for now. Using RO water I have been feeding full strength on the nutrients and have been working on backing them down a bit to compensate for the starting point of 325 now. They have continued to be fed once a week roughly and supplemented with calmag and microbes. Little more detail....... Jan 16/21, Day 36 Veg - 3L each of full line at full strength in RO water - 1150ppm and 5.8pH given to the girls - didn’t add any other nutes other than IPP line. Jan 17/21, Day 37 Veg - dry out day - pulled down all the girls again tonight to train them outwards. - Jan 18/21, Day 38 Veg - 3L for each girl today with tap water and CalMag @ 1ml, Microbes @ 2ml, Nature’s Candy @ 1.5ml - 700ppm and 5.7pH is what that works out to. - have to use a considerable amount of pH down to adjust the tap water, over 6ml in 16L Jan 19/21, Day 39 Veg - measured the girls today, GSCHB Is 12” tall. They were topped already😀 - dry out day for the girls. - Jan 20/21, Day 40 Veg - 2.5L each with plain tap water - 295ppm and 5.8pH. - all the girls are looking big and bushy today.......they are ready for flip soon. Jan 21/21, Day 41 Veg - dry out day for the girls. Jan 22/21, Day 42 Veg - pulled all the girls down again......repeating the same LST each day manually rather than tying down for now. - this should be water day but leaving for today as I am going to lolipop the lower branches and a heavier leaf stripping tomorrow. - will feed tomorrow as they will benefit more. Very solid week ETS......the girls are taking off and expect to see lots of leaves popping out over the next couple of weeks😀👍 Work on the side branching to come up higher closer to the tops next week.......continue tap water and give a feed with Epsom Salts new week

Casey's Rollex OG is doing a lot better then last week. She has found the solution, and has started growing. She is looking good, and will be up for training soon. Nothing more to report at the moment. Thank you Spider Farmer, and DutchFem Seeds. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g