1/22 Week 4! Folded in some CalMag temporarily for a slight def showing up in Jool, foliar of same on all. Recovering nicely from topping will decide on 2cd later Jool recovered her normal color in just a few hours after feedings. Can pull the Cal-Mag for a while. Thought of increasing nuets a tad to 10ml but no need to rush just may have to hit them with Cal-Mag once in a while. Time to hit the roots again 10ml/gal Kangaroots one feed only 1/25 Leaving in a small amount - 2ml/gal - of Cal-Mag with CocoTek more for peace of mind than anything else Keeping them small till I have a place to flower then... then we pour it on. 1/27 Continuing to walk the line between keeping them healthy and keeping the size down - working ok so far Growing nicely although some concern found on Zhaan, feeding pattern on just the two tops shown, no where else. Nothing seen , sticky trap put up will check often for further. Not grabbing the Soap just yet see if anything else pokes up 1/28 Sticky trap clean so no flying critters Think hes right about strange leaves but I want to add I may have done some of this during topping. Just from what I see Bit of a conundrum the plants are near-ish to the size we wanted to flower them at BUT there is no room at the Inn, (sativas...) so we have one path to getting this done. Keeping them as small as we can without risking defs for another week then beginning the flowering in the veg tent Moya while the sativa finishes in 2 weeks. Thats one week of flower in the small tent at best, not sure about that. I swear two tents and one is a 4x4 and still space problems... good to have that problem too 😃 That's the plan but we know there is about a ton of crap that will go wrong like that sativa taking longer. Re-eval in a week Feeding remains as before 1/29 Pic and vid to end week no further issues and the tent is now full. Good problem to have but its convinced us that flowering in Moya is problematic Raised lights to 24" to see if they will go up instead of out for a while. Defol the bottom end of the plants just a bit in attempt to encourage some vertical growth with that as well.

The plats are not been trained, defoliated or anything, grown naturally. I’m blown away how fast this plants are growing. First time outdoors and the experience is very nice, I love the fact it requires little to no effort, in the setup I have chosen. As it is raining a lot, I’m using only a dry amendment I have built to use on them periodically, this way I’m not adding more liquids to the root system that is already highly saturated, essentially I’m using the following recipe update, I don’t know if it is correct but I live it all mixed. Use about a table spoon and a half every 15 days. - Azomite 500g - Natural fosfate 500g - Biochar 1kg - Bokashi 1kg -Kelp meal 500g -Mamona meal 1kg -chicken meal 1kg -Potassium silicate 500g -Gypsum farm 500g -Diatomaceous earth 500g -Bunny guano 300g -Bat guano 1kg -Oyster shell powder 1kg -Cow blood meal 1kg -Cow bones powder meal 1kg. -Wormcastings, spare use not in the mix. The plants are showing good coloring, no fade and no deficiencies, the green is not to deep, nor too light. As the leafs are taking a lot of water and strong sun and wind, the leafs are feeling a bit. As the wind is high since day 1 the stems are very resistant, strong. The Fastberry Is on the smaller size if compared to the other plants, together with the Banana purple punch, then we have the Bluedream and the CBD crack on the larger sizes thriving a lot, I expect to approach the end of flowed in a few weeks, the girls are very fast indeed. I’m looking forward to collect the fruits :)

Day 56 New Rez change, partial drain and RO fill up. 125 Liters. 200PPM, ph 5.9 First day flush. Will flush again in 3 days to get to 80ppm. Day 59 Drained res, added florakleen and RO... PPM sub 100. Day 61 Drained the reservoir, added 100L new RO water, no florakleen. Pure RO at 20 PPM. Should be the last drink they get. I was going to harvest tomorrow on day 62, but trichrome inspection is only showing about 5% amber. I wanted to try and get a little more ambers this run. (Note for next run, keep nutrients till day 63, then start flush). Seems to be slower to ripen with this phenotype, hopefully not too much longer. Will chop Monday likely, possibly Tuesday... who knows!? Also, dropped lights on to like 10.5 hours and lowered the light wattage to the minimum.

Really coming along nice trying to get established in case I am called away for work or anything else over summer ideally these ladies will continue getting fed as long is there given clean balance water I’m going to hit em heavy with seabird guano at flower, probably crank up the molasses on that end, really just properly letting water sit oxygenated mixing and letting solutions hit microbial levels that put the real work in, I’m hitting with a nute I’m making myself 3 times a week at point all water is being carbon filter dechlorinated the aerated then to casting or mycorrhizae brews that I rotate my mycorrhizae brew is Bigfoot water soluble and dechlorinated water except I do more then recommended ok on the each time. Results are stunning my cover are dense earthy and rich bio smells tons of spiders and other good predators a large toad made a home in the clover also he must be chowing cause he’s healthy. I will be making a drive and coming back with a bunch of nutes I’m picking up out of state as well as some Carolina mantis cocoon I’m staying up on pest this year. I’m steady rotating neem bt since birth I’m not fucking around this time. Crank up concentration slowly but hitting light preventative pest spraying at least twice per week as it get warmer I’m gonna be in a constant panic about the stupid moths. Plants drink a lot of water already and technically my outdoor season has not begun for most around me till Mother’s Day. I’m already 11 weeks deep. Cages are rough on leaves but I’m not to worried about the ladies are Strong a week of cages they Wii all be locked hugging there cage in place. Something in particular that’s rather stunning is the leaf quality health of all strains, it’s def what I’m doing with the soil. it appears that microbiological forces at work influences aspects of the plants of which results I’d yet to receive. The greenness and the crisp leaf sound when the leaf’s brush against something. Getting REALLY Excited about things! near perfect start. My big healthy wendigo not the cripple is def a top perform when I comes to sheer mass that is because that plant is super definitely tallest sitting at about 2ft. They are topping and splitting real well, black phoenix v2 isn’t performing to my expectations so far, though it’s new branches that coming in will be game changer come solstice. So we are now over month and a half left of healthy veg and flower stretch on these, all things willing I’m betting three these size ration looking for. (12ft or bust) Hoku seems to be great seeds for people trying to do outdoor for quantity. Mind you I have yet to see the flower this is mere speculation from what I’m witnessing of its veg time. The gluey c99 though.. that plants a straight beauty. Short but kicking perfectly shaped little sativa shaped hybrid leafs this plant took to topping perf one the best veg ladies I’ve ever had In this garden so far. Not the tallest but a stunner none the less. The snow jokez are really going for the gain lane too both are topped real well but one particular was my earliest successful top I was able to establish clean two cola fresh off the rip. Perfect fork Excited if these end up becoming trees all these stems and tops are gonna be bursting out the seams. Really want to find something cool to make our massive outdoor cannabis stem knots into one of days if anyone seen any thing cool holler fs. So I did not anticipate the plants flowering so quickly and will be monitoring the situation in the case I need to remove small flowers with a nice top on everything going two weeks or so into may. Got small bud sites developing on every plant.

Hard Camo strain

9/22 topped them at 4th node. some of them are looking better for mainlining that others. some of them i may not mainline and others i left an extra node in case i want to keep clones around. #1 is looking happy with the topping and looks really good. #2 is the only one thats been praying and is the tallest and strongest looking all around. #5 short and such tight internodal spacing that it makes it hard to see whats all there. just looks like a bunch of small leaves until they grow out. i may not mainline this one either be cause of it being so tightly packed it may make it 9/27/24 day 20 theyre growing quite well. #1 i ended up taking the bottom nodes. i was originally goi g to use them as clones later on but decided to just let her go with her 4 nodes. #5 is so tightly packed, she has like 8 mains all in such a small space, its hard to even make sense of it all.

5. Woche

❄️ The Frozen Archive #2 | LSD From Fresh Frozen to Temple Ball Hello GrowDiaries family! 👋❄️🌱 Welcome back to The Frozen Archive. For those joining the journey for the first time, this series follows our exploration of fresh frozen material, ice & water extraction, solventless concentrates, curing techniques, and everything that happens after harvest. If you would like a complete introduction to the Bubbleator machine, fresh frozen material, and the fundamentals of ice & water extraction, I highly recommend checking out The Frozen Archive #1, where the entire process is explained in detail. This entry focuses on a specific genetic: LSD by Zamnesia. And what a beautiful plant she was. For anyone curious about her journey from seed to harvest, the complete cultivation diary is available on my profile. This report picks up where that story ended. The flowers were harvested. The material was preserved as fresh frozen. And now it was finally time to see what LSD had to offer through ice & water extraction. ⸻ A New Wash, A New Lesson Although this is only the second entry in The Frozen Archive, this extraction actually represents my third wash using the Bubbleator machine. The first archive introduced the machine and documented my first experiences. This time I approached the process with a little more confidence, a little more understanding, and a lot of curiosity about how LSD would perform. One of the goals of this series is not simply to show the results. It is to document the learning process itself. The successes. The mistakes. The observations. The improvements. And hopefully create a useful archive for both the community and my future self. ⸻ The Material Unlike trim runs, this extraction was performed using fresh frozen flowers. Entire buds were harvested, frozen immediately, vacuum sealed, and preserved until extraction day. By freezing the material directly after harvest we aim to preserve as much of the plant’s original profile as possible before beginning the washing process. Months of cultivation eventually lead to this moment. The plant changes form. But the journey continues. ⸻ The Wash The process itself follows the same methodology introduced in Archive #1. The Bubbleator was loaded with: ? Ice 🌿 Fresh frozen LSD flowers 💧 Cold water The flowers were placed inside a properly secured 220-micron wash bag, positioned inside the machine, covered with additional ice, and submerged in water. From there the machine could begin its work. One thing that quickly becomes obvious is how important temperature control is. Throughout the wash I monitored the water carefully and aimed to keep conditions as close to 0°C / 32°F as possible. Cold temperatures help maintain trichome integrity and improve separation during agitation. Patience becomes one of the most important tools in the room. ⸻ Watching The Transformation One of my favorite moments remains watching the water change. At first everything appears clean and transparent. Then gradually the water begins taking on a different appearance as trichomes and plant material enter suspension. The Bubbleator quietly continues its work while the ice maintains the environment. And while the machine washes… Well… The grower medicates. 😄🌱 Some traditions are important. ⸻ Collection & Multiple Washes Once the cycle was complete, the water was drained through the filtration bags. This is where the magic becomes visible. The resin that was previously attached to the flowers begins collecting inside the bags. The first collections looked extremely promising. As always, multiple washes were performed. Each wash tells a slightly different story. Eventually there comes a point where the material being collected becomes noticeably greener and contains more contamination than desired. That is usually the signal that the best material has already been recovered. At that point it is time to stop chasing quantity and preserve quality. ⸻ Freeze Drying… Dog Doctor Style After collection, the resin was transferred for drying. This particular batch spent approximately five days drying in the freezer before reaching the condition I was looking for. Once sufficiently dry, the material was broken apart and worked into smaller pieces to increase surface area. It then spent additional time drying before the next stage could begin. The goal is always the same: Remove moisture. Preserve quality. Remain patient. ⸻ Working The Resin This is where things become truly enjoyable. Once dry, the resin was carefully worked and mixed together. The purpose is not simply aesthetics. The goal is to create a more homogeneous material where everything is evenly distributed. The process requires patience. Heat from the hands. Gentle pressure. Time. Slowly the texture begins changing. The colors become more uniform. The material becomes increasingly workable. And eventually… The temple ball begins to emerge. ⸻ Temple Ball Formation Watching loose resin transform into a temple ball never gets old. What begins as dry collected material slowly becomes something completely different. A smooth surface develops. The texture evolves. The appearance changes dramatically. And suddenly the extraction takes on a form that has been used for generations. This batch produced a beautiful temple ball with excellent visual appeal and impressive characteristics. ⸻ The No-Oxygen Cure Experiment This entry also begins a long-term experiment. Part of the material was placed inside parchment paper, sealed inside a vacuum bag, and stored without oxygen. The objective is simple: Observe. Learn. Compare. How will the resin evolve? How will the aroma develop? How will the texture change? Will it butter? Will it remain stable? Will the temple ball age differently? Only time will answer those questions. For now the material continues resting. And we will return in a future entry to see where that journey leads. ⸻ Early Impressions Although the cure is far from complete, early signs are extremely encouraging. The material melts beautifully. The bubbling behavior is exactly what I hoped to see. The aroma remains rich and complex. And after a quick three-week check on the vacuum-cured material, everything appears to be moving in the right direction. Now comes the hardest part. Waiting. ⸻ Looking Ahead This is not the end of the LSD story. This is simply the beginning of its next chapter. The temple ball continues curing. The vacuum-sealed resin continues curing. And in a few months we will return to compare the results and see how time has transformed the material. For now, I am extremely happy with the outcome and excited to continue learning. That is ultimately what The Frozen Archive is all about. Learning. Documenting. Improving. And sharing the journey. ⸻ P.S. A huge thank you to Zamnesia for the LSD genetics and for the Bubbleator machine that made this exploration possible. And a special thank you to Plagron for supporting the cultivation side of this project. The LSD plant documented here was grown from seed to harvest using the Plagron lineup, including Terra Grow, Terra Bloom, Pure Zym, CalMag Pro, Green Sensation, Power Buds, Power Roots, and Sugar Royal. The flowers became fresh frozen. The fresh frozen became resin. And now the resin begins its cure. Everything is connected. Grower’s Love everyone. 🌱💚❄️📚

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Have fun with the update. Flowering day 33 since time change to 12/12 h. Hey everyone 😃. Another wonderful week goes by :-). The lady is developing very well. The flowers begin to develop properly and already smell very nice 🙂. The day before yesterday was the last time 1 g of GHSC Powder Feeding per L substrate was added :-). That should last to the end now. Of course, she continues to receive GHSC enhancers 👍 every 14 days. The tent was cleaned every day just like the humidifier was refilled every day. I am very curious how it will develop in the coming week and until then I wish you a lot of fun with the new update. Stay healthy 🙏🏻 and let it grow 🍀🙏🏻 You can buy This Strain at : www.Zamnesia.com ☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼 Strain Gelato clone from mother (Zamnesia ) ☝️ Genetics: Wedding Cake x Gelato x Gelato 33 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

Still a seedling, but moved to the main pot.

-

Not much to be said for this lady. Her calmag issue seems to be still present and I'm suspecting lockout. She's on pHd water and calmag for now. Her aroma is amazing. So minty but cheesy at the same time. Amazing. She is fattening up good regardless of any issues. Not long to go for this girl!

Habe am gestern am 23.12.2024 15l Wasser gegeben heute 10L

HPS Lamp 600w GORILLA growing wel and fast. 🙏💯

Commencing Week 14 - 6th week of flowering - Day 92 from germination - Day 42 of 12/12 on January 23rd D.98/48 wooooooooooooooooh this Garden is super stinky right now in a good way 👃💪👍👌👏😁 The colors are changing up beautifully signaling the end of life and the beginning of a whole new level of appreciation for these beautiful flowers 💚 - 💛🧡💜❤️ I'm currently under the impression that hydroponics can speed up production, (in my experience with organic growing growth is a little slower and as well as the ripening / muturation rate) so I'm may even be able to harvest a majority of this a few days early. There is also a few tricks I might employ in order to speed this process up. It's fine by me either way I can wait so we'll see 😁 So far this has been my first successful indoor DWC grow in quite some time and I am very patiently yet very anxiously awaiting the time when I can enjoy the fruits of my labor. at this point as long as I don't mess anything up it should be smooth sailing right up to the very end which is now what I am looking forward to the most coming up: the harvest and smoke report To all the breeders so I put in the hard work for for me and allowed me to grow their strains thank you! To all the fellow growers and enthusiast who have been paying attention and chiming in from time to time I especially appreciate you and thank you for your patience in dealing with my late updates. I promise my smoke report will be incredibly thorough and make up for your patience throughout the recording of this journal. As usual if anyone has any questions about any specific techniques I use or why or nutrient lines or strain related to this grow information please feel free to ask, comment, etc

Muy buenas!! Asi va esta bonita cepa! Muy indica y chatita ahunque ahora que tenemos menos horas de luz se ve como va estirando mas. Ya se ven los primeros pistilos, vamos a ir viendo como se forman esas flores!!

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Will update once I have dried and smoked.