2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Alguns ataques de bottritis e corte no top Bud mais consegui controlar com desumidificador alimentando com( ripen ) GHE desde o dia 50 caminhando para a colheita

Amazing week. My first ever Low stress training! LST began on day 16. Growth slowed some for 1 day. I adjusted ties each day since. On the 2nd day it improved growth. After 3 nights they are back on track to full growth. The stems have really thickened and tons of new growth between each branch of each plant. Day 20- I began running the carbon filter system. I have been running intake air for a week timed. Now it is on full time on low pulling through the carbon filter out of the tent and low air intake inbound aiming at the plants. 2 vent doors are open to create what appears to be perfect negative pressure. (Slight suction, not intense) conditions have now become very stable. Plants received nutrients this evening. 15ml of fox farm big bloom and 5ml of fox farm grow big to 1 gallon water. Final pH 6.4 this round Day 21- absolutely amazed by the filtration system. Maintaining consistent temp & humidity. Plants received a dusting of nutrient water to re wet the top of the growing medium. Nutrients were aerated over night before the dusting. pH increased to 6.7 which I left. They will remain un-touched for the next 40+ hours.

Tag 77 / Blüte Tag 17 Endlich ist das Fenster zu gebrauchen Die Pflanzen stehen hervorragend da. Der dreistellige Zentimeterbereich ist geknackt – 1-Meter-Marke erreicht! 🎉 Allen voran die White Gushers 33, die aktuell minimal die Nase vorn hat. Die Kush ist etwa einen Zentimeter kleiner, wirkt insgesamt etwas heller und noch nicht ganz so weit. Die Gushers zeigt sich dagegen schon deutlich weiter, was Reife und Lichtreaktion angeht. Der Stretch läuft spürbar aus. Genau so, wie er soll. Ein paar Zentimeter dürfen es von mir aus noch sein – aber bitte jetzt nicht mehr eskalieren 😅 Meine Wunschhöhe lag eigentlich bei 96 cm😉, die ist inzwischen überschritten, aber gut: Das Zelt lebt noch, ich auch. Ein Umknicken möchte ich nach Möglichkeit vermeiden, und aktuell sieht es so aus, als würde die Energie jetzt endlich in die Breite und Struktur gehen. Erste Anzeichen einer Stretch-Verlangsamung sind klar erkennbar. Blütentechnisch sind wir bei Tag 17. Die Pflanzen sind inzwischen deutlich mit Härchen (Pistillen) versehen, die Blütenansätze sind klar sichtbar – und genau deshalb warte ich mit dem großen Eingriff noch ein paar Tage. Der Friseurtermin ist aber fest eingeplant: Es wird Zeit, die Klingen zu schärfen ✂️ Die Entlaubung wird kein Spaß, da ist ordentlich Material dran. Ziel ist ganz klar: Luft, Licht, Fokus auf die Blüten. In der kommenden Woche steht dann auch wieder eine Mikroben-Gabe an. Ich bin sehr gespannt, wie sie darauf reagiert – idealerweise genau dann, wenn sie voll in der aktiven Blütenproduktion angekommen ist. Einen weiteren Wachstumsschub brauche ich jetzt definitiv nicht mehr. Jetzt darf sie liefern. Und dann dieser Moment: Das Fenster im Growzelt macht endlich Sinn. Man schaut rein – und sieht nicht mehr nur Grün und Stretch, sondern direkt davor echte Blütenbildung. Das ist diese Phase, auf die man Wochen lang hinarbeitet. Eine richtig schöne Zeit im Grow. Ich freue mich sehr auf die kommende Blütephase. Jetzt beginnt der Teil, für den man das alles macht 🌸✨ Finally, the window actually makes sense” The plants are looking excellent. We’ve crossed into the three-digit centimeter range — the one-meter mark is done! 🎉 Leading the way is White Gushers 33, which is currently just a bit ahead. The Kush is about one centimeter shorter, slightly lighter in color, and overall doesn’t seem quite as far along yet. The Gushers, on the other hand, already looks a bit more advanced, especially in how it’s responding to the light. The stretch is clearly slowing down now — exactly how it should. A few more centimeters would still be fine, but no more wild growth, please 😅 My target height was originally 96 cm, which we’ve now unfortunately exceeded. Still, everything is under control. I’d really prefer not to bend her over again, and right now it looks like the energy is finally shifting from vertical growth into width and structure. The first clear signs of reduced stretching are definitely there. In terms of flowering, we’re at Day 17 overall. The plants are now clearly covered in pistils, with visible bud sites forming — which is why I’ll wait a few more days before doing the major cut. That said, the hairdresser appointment is already scheduled: the blades need sharpening ✂️ There’s going to be a lot of defoliation. Plenty of material on these plants. The goal is clear: better airflow, better light penetration, and full focus on flower development. Sometime next week, the next microbial feeding is planned. I’m really curious to see how they respond — ideally right when they’re fully locked into active flower production, so they can really use that boost. Another growth push is the last thing I want at this stage. And then there’s this moment: The grow tent window finally makes sense. You look inside, and instead of just green stretch, you now see actual flower formation right in front of you. This is the phase you wait weeks for. A really beautiful part of the grow. I’m genuinely looking forward to the coming flowering phase. This is where it all comes together 🌸✨

A lot going on this week!! Feeding 3 quarts every Sunday and Wednesday at this point. I did a light defoliation on Monday and did a more thorough defoliation on Friday! I opened up a lot of bud sights and it shows in the pics from late in the week!! I just turned the fan and carbon filter on. Temps are currently holding at 73-74F and the humidity is between 43-45%. I'm very happy with that! I hope to keep them both happy!! Enjoy!

She is going fantastic... Great Citrus- Earthy smell... The grow is Bio... she is not drinking a lot... fingers crossed everything goes like this untill the end... 🙏

Did some cuttings on her, found some bugseggs not much It's been raining a lot but she won't be up for very much longer and I still hope for some sunny days😘

Transfer to3 gallon pot

Topped my little babies. Transferring them into bigger pots soon and will begin lst.

Que pasa familia, vamos con la quinta semana de floracion de estas Lemonpaya feminizadas de fastbuds. Vamos al lío, de las 3 plantas, me quede con 2 por espacio, siempre pongo alguna semilla de más por si no abriese alguna por no perder ese hueco del indoor. También se trasplantaron a su maceta definitiva, en este caso de 7 litros que además provocó un shock que también solucione. El ph se controla en 6.2 , la temperatura la tenemos entre 20/22 grados y la humedad ronda el 50%. Las flores empezaron a formarse y progresan a buen ritmo. No entiendo que a sucedido , uno de los 2 ejemplares tiene un bloqueo severo de nutrientes, estoy controlando el Ph correctamente y la alimentación ración pues corte a ver si vuelve a reestructurarse. Hasta aquí todo, Buenos humos 💨💨💨

Everyone is thriving! Things are going fairly well. Had a rough start but now everyone is living their best life! 🤩

@FAST_BUDS, Hi all the happy people here in GrowDiaries. This is the new release of Fastbuds first CBD strain. https://2fast4buds.com/seeds/cbd-crack CBD Crack is a superchill euphoric hazer and medical marvel, rapidly becoming our anytime favorite. Perfect for wake & bakes, this autoflower strain guarantees a mellow, positive and focused high. Crack CBD is FastBuds' latest medicinal wonderbreed, delivering a rock-solid 6% CBD index. These distinguished, colorful plants autoflower beautifully in pine tree style. Lovely purpling and abundant frost adorn quality buds from top to bottom, with harvests of up to 250g per plant within 75 days of seeding. TASTE & AROMA Its distinctive flavor combines rustic and pungent tones with hints of citrus and cinnamon. THE HIGH EFFECT Contrasting robust CBD with lower THC, this bud induces a delightfully soothing, uplifting, low-anxiety effect which allows brain and body to function, while instantly easing symptoms and struggles. HOW IT GROWS CBD Crack boasts vigorous early growth, with full blooming around week 5-6, good frosting with a nice scent by week 8, and flowering in week 10 or 11. Ponytail her to get the side branches out & up and form some nice colas. Try 500ml of feed every other day, from day 14-17 up to week 4, and increasing as required thereafter. 70cm is a good height to reach, resulting in around 120 grams of bud weight. We recommend a bit of Low Stress Training to improve light penetration, and experimentation with light defoliation. ----------------------------------------------------------------------------- 2017-10-09. Week 8 starts and the girls are at day 55 from germination. The nr 1 is 61 cm high and stacking up in the budsites and smells sweet, blueberry/rasberry and nettles. Nr 2 is 39 cm high and smell sweet, lemon, berry, nettels and mint. One of them have som nuteburn in the tips and the second one i burnt the top on the hps lamp when i was replacing it in the room. They drink about 3 liters every day and are frosty and sticky. This is a awesome girl to grow this far and they look and smell beautiful. What i have seen here in GD, this strain is harvested between week 9-11 so its getting closer.

Day 52: La mia adorabile cream cookies è entrata finalmente in fioritura, sicuramente avrò avuto qualche parametro sbagliato, ma sembra andare tutto bene anche questa settimana, tardiva ma comunque soddisfacente. Adesso nessun errore e si comincia a volare 😍💚

So this is day three of pre vegetation that means the seeds have not yet broken the soil completely and grown their first true leaves. Not much to really say at this point they all are growing roots and doing very well so far. I'm just using tap water at this point. An important note to make these are seeds from a company called 7 East genetics here in Ontario Canada they shipped me my seeds in good time although Canada Post was slacking LOL. Can't wait to see how these babies grow and I very much hope they live up to their name.

Ist alles perfekt ph ec ist stabil

Week 7 flower

A great week for the sherberts. They have been topped and pinned and are growing out great. Always praying always happy. I wish my kids were like these two!. No dramas to report. They seem to like any enviroment they have been subjected to in order to keep other plants in the grow space happy. They take the cold just fine. they love the warm also. Only thing i found is they had issue sooner then others with RH over 70% ( i was running high RH to fix my Chibas) #1 has bee cleared of nodes 1 and 2 and will have another topping in 3 days or so. #2 i plan to grow out node 1 cut 2 and top 3.. Giving 12 colas in total after final topping. Started feeding some grow to them at an EC of 1.25. I was planning to repot but they seem okay atm. Maybe next week. I also dropped 2 hrs off the light scehdule so they are running on an 18/6 from 20/4. Added a little vid of the grow room ATM😅👍 Thanks for stopping by. stay safe.. Grow well.

Girl Scout Cookies Auto was suddenly ready to get chopped - I would've like to see a few more amber trichomes, but the fan leaves started yellowing faster, and more sugar leaves got crispy tips. Also, the smell got pretty strong over the last week or so. It was a very nice grow, and I'm curious on the yield and effect after drying and curing. Yuhbar Auto still needs a little more time, and will enjoy a few more days or weeks under the (artificial) sun, giving the trichomes more time to ripen.

Day 39 - Gave 1.5l with half bloom nutes each Day 41- Rotated the plants and swapped them to expose even light across the whole plant and watered 1.5l with water and calmeg @ph6.3 Day 44 - Defoliated and did some lst and watered with week 1 bloom nutes @5.8 ph