Continua il tragitto verso metà della fioritura e ieri sono state sciacquate solo con acqua a ph neutro. The Sin è la pianta più alta di tutte con foglie più strette presentando caratteristiche più Sativa..quando entro nel box sento odori buonissimi 🤤 da rimanere lì ore per cercare di catturare più possibile ogni sfumatura della sua fragranza 💜non resta altro che aspettare 🌱👌

I think 2 a 3 weeks more before harvest

So this week i will be repotting the ladys in 5gal fabric pots with clay pebbles and a mix of biobizz light mix soil and all mix soil. I will be starting to use 4ml/1l of water of biobizz roots. I'm thinking of doing a 6 week veg on these ladys as I will be doing a 9 plant auto run in the 4x4. Happy growing guys.

Buenas noches fumetillas, abro ya mismo el diario de las green poison de sweetseeds, una variedad con una floración rápida que crecerá bajo unos Leds de la marca mars hydro. . Quitando el equipo el control básico es ph 5.8 temperatura 22/24 grados y humedad 70/80%. . La alimentación de la gama agrobeta. 0,5 ml x L Piramid , vía radicular. 0,5 ml x L Growth black line , vía radicular. 0,1 gr x L Cancerbero , vía radicular. 0,1 ml x L Tucán , vía radicular. 0.1 ml x L Flash Root , vía radicular. 0,4 ml x L Great Green , vía foliar. . Para ser la primera semana esto es todo en verdad, un saludo y buenos humos 💨💨💨

Flower day 40

On day 1 the PH of the reservoir is 6.1. I changed out my reservoir. My tallest cola is 3.25" from the light. Lower colas are mostly 7"-9" from the light. I'm still getting runoff on my right plant at night maybe the low humidity drying out the pots too quick? I decided to lower the flow rate by 1 carrot (total of 2 carrots tightened since first runoff event). The leaves are starting to produce more trichomes :) On day 2 the PH of the reservoir was adjusted the PH from 6.2 to 6.1. My tallest cola is 3.25" from the light. Lower colas are mostly 7"-9" from the light. I'm starting to smell terps when the leaves are rubbed it and smells good :) On day 5 I raised my reservoirs PH from 5.8 to 6.2. I increased my blumats flow by 2 carrots. I dropped my new light on my plants splitting the trunk of the right plant. I used duct tape on the wound and the plant seems okay so far. I smashed a few top colas accidentally and hope they will be OK. I have decided to install the new light after this run when there are no plants in the box. On day 6 The reservoirs PH is 6.2. I installed my new AC infinity carbon filter and increased inline airflow to keep up with smell and humidity/transpiration.. The plants are smelling very nice. The trichomes are increasing and the buds are growing in size. So far the damaged plant shows no negative consequences 😅 On day 7 the reservoirs PH was 6.0 so I adjusted it up to 6.3. I increased the flow rate of my blumat on the right plant by one more carrot bringing it back to its original state before there was a runaway. I turned off my dehumidifier and will monitor my humidity/temperatures going forward.

thanks for the comments guys ! im switching to 20 hour of light tomorrow

Vamos familia actualizamos la cosecha de las gelato Olandese de Dutchfem . La verdad que el secado muy bien 7 días en Malla y a los botes, 40% humedad y 24 grados es la temperatura ambiental que han tenido en el secado. Por lo demás de miedo os la recomiendo. Gracias a DutchFem, Agrobeta y Mars hydro , sin ellos este proyecto no sería igual 🙏. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Buenos humos.

The plants continue to kick ass. New French Macaron and Chem Brulee leaves are all 9-fingered now. Crazy happy plants. Sprayed with light BT spray (trying to avoid any caterpillar issues this year as much as possible) early in the week. Sprayed with light Neem Oil / Castile Soap (no more dawn, no more issues!) late in the week. Today, June 9, I also defoliated a bit. Keep in mind that I don't want to do much this year as far as topping and training, so I've done one simple top on all of the plants, and essentially have created 4 main colas for each plant. I only did this to create some airflow under the plant. I don't plan on doing a lot of this stuff. Gonna let the sunshine do her thing. Even though these girls have some hot-ass super soil in there, I'm still going to be doing some top-dressing. Mixed this up and slathered it on there on Saturday, June 12. ------

She si a shorts Girl with fat colas, the smell is strong and sweet, the flowers are very hard and sweet, My Main complaint with this strain is the time, because the bank says si a 8 week strain but she doesn't.

Tent is filling up nicely now, they are looking perfect not a sign of stress or deficiencies. Will do some more training and leaf tucking in the next couple of days. 19/7 Done I light defoliation and leaf tucking and a bit of moving around to fill the canopy as much as possible. They are looking super healthy considering that I'm only watering with molasses added. 27/7 looking lovely now starting to flower profusely now. And the smell is really amping up.

Very Tasty!!

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

did not get wet weight on this one but will update dry weight in a few days

The outcome of the flowering was very surprising. Flowers pumped up the last 2 weeks, became hard without leaves inside the buds, developing an amazing rancid and musky smell . During the vegetative phase; the plant grew vertically with pretty good ramifications..I did a topping very late, just before flowering like in emergency to stop the stretch: the plant was a little bit shocked but never stopped growing with a big internode’s distance. The opérations finally gave a good result: forming and developing more blooming sites. But the whole stability of the structure was changed and the plant was bending dangerously till the harvest.

Looking good. Top dressed 15% worm castings

23 Dec - Evening @ 8PM - Excited! Third grow here we go (whilst simultaneously running my second grow into flower & a third outdoor started)! 24 Dec - a lot of seeds popped already. Will wait until morning to put in soil with a longer/stronger root. 25 Dec - 36hrs from putting seeds in water - all seeds now germinated & planted in moist soil, high perlite % (approx 50% and minimal nutes), temps between 25-28c, on 18hr light cycle, 50% on edge of light strength (see photo) and lightly misted with ph’d but nute free water (will be doing daily) in humidity dome with vents 100% open, good air flow around). N.B. Mimosa x Orange Punch had fallen out of shell already. Will see if becomes a problem now it’s in the soil. Planted with coldeyns just on soil line with fine soil v v lightly dressed to cover. Decent root so hopefully will be okay. 26 Dec - everything looking okay and misting pots and dome every 12 hrs (AM & PM). Vents mostly - 75% - closed, with fans going in the tent. 27 Dec - signs of life with all seeds still - most just about to pop through soil line (AM), now having popped above soil line (PM). Still misting every 12 hours and photos above just before another misting. Working well. So happy. First grow 25% success rate, second grow 50%, so hoping for a 75%+ this time! Really putting the time and trust in, not overwatering (past mistake) and using humidity not trying to water non existent root system (caring too much)! 28 Dec - tracking nicely! CLONES TAKEN FROM TRIPLE CHEESE AND RUNTZ MUFFIN (rooted by 17 Jan). 29/30 Dec (1am!) - further progress. Wld lyk to see Charlotte’s CBD above soil pls! 30 Dec (PM) - getting there! Choc mint, fat banana & Mimosa x Orange Punch into veg now, with Frisian Duck, Gorilla Zkittlez, SAGE CBD above soil but waiting on seed head to fall off and CBD Charlotte’s Angel to come above soil line. Sunday tomorrow. Might start ‘veg’ on Monday for simplicity and the hope others will be up and away!