2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Update week 4 Bloom

La semana empieza con una pequeña poda de hojas para airear un poco y regando solo con agua hasta la cosecha. Esta semana seria para limpiar un poco el sustrato de nutrientes , sin más. Una limpieza en dos o tres riegos.

Final stretch for the Cheese trees twins. Amazing smells inside the grow room. Smells great, but this dank ass smell, lingers in the air. Not sure which is creating this odor, or maybe just a mixture from all the ladies. Straight water for the next week. Once I get them down under the 200 ppm mark, I will be dropping these ladies. The tops of these ladies are super heavy. Light cables are stopping them from bending right over and hitting the floor. These Cheese have been healthy through the entire grow. Haven't lost a single leaf and all remain healthy. One healthy strain and a pleasure to watch grow and bloom. Looks like it will be a breeze to trim up.👍

The main buds are really starting to develop and it looks pretty healthy. Some rusty spots developing on some of the older fan leaves. Added a pick if anyone knows what it might be? Big fat calyxes starting to appear frosty with trichomes. Defoliated a few leaves, may have over watered trying to fix the cal mag issue and the damaged leaves were a bit wilted. Did one big final adjustment to her shape to open her up. Was concerned about how dense it was getting and light and air penetration with rainy weather coming as it buds out made me want to give her some breathing room.

I topped some of the ladies that were getting a little bigger than the rest of the plants hoping it slows down some of the growth so the smaller plants can catch up thanks for reading😏

Harvest time! Finally! Super happy with this grow! I'll update harvest with pics of jarred buds after it dried 💚 Last values were 200 ppm💚

The Harvest Prelude: After a patient wait and 48 hours of darkness, the moment arrived. The Tropical Tangie Lego Ninja, standing proudly like a silent guardian, was ready for the grand harvest. The sound of each cut echoed through the grow space, reminiscent of felling a mighty tree. The compact structure of the buds was awe-inspiring, a testament to the ninja's disciplined growth. The Lego Ninja, like a stoic warrior, was then hung upside down, beginning a two-week drying ritual. Maintaining an ambient temperature of around 21°C and a relative humidity of 60%, this slow-drying process allowed the buds to retain their essence, preserving the symphony of terpenes and cannabinoids. The trimming phase unfolded like a fragrant masterpiece. Each bud, adorned with a glistening layer of resin, transformed the trimming process into a sensory delight. The zesty aroma of oranges enveloped the room, a tangible expression of the tropical lineage. The compact buds made every snip a careful dance, revealing an abundance of frosty trichomes. The moment of reckoning arrived with the scale's unveiling. The Tropical Tangie Lego Ninja, from a single plant, bestowed upon me 309 grams of dried medicine. Each gram was a treasure trove of cannabinoids, a promise of euphoria and relief. The sheer compactness of the buds translated into a concentrated potency that left me in awe. In parallel, the clones, nurtured with equal care, showcased a fascinating divergence. A twist in the tale unfolded as the intense oranges of the mother transitioned into vibrant tangerines. This unique expression, I believe, was a testament to the clones weathering a summer heatwave differently, bringing forth a distinct personality. The clones, resilient and generous, gifted me a bountiful 549 grams of pure joy. This now my keeper of the garden, an embodiment of the Lego Ninja's legacy. The harvest dance, though familiar, carried the nuances of tangerine zest, a refreshing twist that left me eager to explore its intricacies. Both harvests shared a common thread—their resinous abundance. The trim bin, akin to a treasure chest, gleamed with golden trichomes and fragrant joy. Sticky fingers became a badge of honor, a tangible connection to the resinous richness that filled the room. Shout Outs: No harvest report is complete without expressions of gratitude. A special nod to @DutchPassion_Official for crafting the genetic marvel that is the Tropical Tangie Lego Ninja. Their expertise paved the way for this epic journey, and I tip my hat in acknowledgment. In conclusion: As the dust settles on this epic harvest saga, my heart brims with happiness and excitement. The Tropical Tangie Lego Ninja has proven itself not just as a plant but as a living masterpiece. Stay tuned for the smoke report, where we delve into the true essence of this botanical ninja's legacy. Until then, fellow cultivators, may your gardens flourish, and your harvests be as bountiful as the joy they bring. As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and blessed with you all in my life<3 <3 <3 #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 Friendly reminder all you see here is pure research and for educational purposes only <3 <3 <3 Growers Love To you All <3 <3 <3 P.S- I must extend my sincerest apologies for the missing video reports. Regrettably, Grow Diaries is still facing some technical issues that are preventing me from uploading them. Rest assured, as soon as the situation is resolved, I'll share those videos to give you an even closer look at this fascinating journey. or in my case apparently GD dont let me do uploads over 100mb with is super small file if you working and editing videos , doing my best here to upload them all but GD is not making my life easy on this <3 <3 <3

The 2 little girls are so cute.i love the evolution😍

Get my scope tomorrow to find out finally what is going on with the trichomes!

Bueno, pues ya se nota que empiezan a engordar y tirar resina, poco a poco huele más jajajaja. Me muero de ganas ya de que llegue el dia del sacrificio ✂️✂️😂🔥. Sobre esta, va oliendo muuuy muy bien, dulzona sin duda, y aparentemente es la que mas corriendo va del grupo, peeeeeeeeero tengo entendido que esta corre mucho al principio pero luego la maduración es mas larga que un dia sin pan. No lo se xD ya lo veremos.

Haven't done any feeding so far, I've been usong miracle grow so I think that's the only reason it's doing okay. I'm buying Remo kit nutrients this week and im excited to see what a difference it will make. But any advice on how much I should do for her first feeding or even what I should start with exactly would but greatly appreciated.

Last week. 9 weeks have pass now 48h of darkeness and then chop chop..

Hello growmies 👩‍🌾👨‍🌾🌲🌲, 👋 Girls grow very well, much than expected, principaly on some stems of #2 and start to be very close to the lamp, Hedgehogs coming along, litle start of overfert, only water this week 💪 Continuing scrog, will do a big def soon. 💧 Give water each 2/3 day 2 l Water Only PH @6 💡Mars Hydro - FC 3000 50% 17 cm Mars Hydro Fan kit Setting 8 Have a good week and see you next week 👋 Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Mars Hydro - Smart FC3000 300W Samsung LM301B LED Grow Light💡💡 https://www.mars-hydro.com/fc-3000-samsung-lm301b-led-grow-light Mars Hydro - 6 Inch Inline Fan And Carbon Filter Combo With Thermostat Controller 💨💨 https://www.mars-hydro.com/6-inch-inline-duct-fan-and-carbon-filter-combo-with-thermostat-controller Fast Buds - Gorilla Cookies FF🌲🌲 https://2fast4buds.com/us/seeds/gorilla-cookies-fast-flowering

De momento todo parece estar bien hasta el momento d semana 4

Harvested on day 113 after ending week 9 of bloom. after washing week ago with adding flora kleen and then after 3 days turn light to 48 hours darkness before harvest. First trim in harvest defoliated big fan leaves with all the leaves without sugar and the tip of the small leaves. When the buds will be dry ill do second trim untill the bud will be like dense diamond. So, really love this strain and i give her a score of 9/10. First, for the buds beauty,dense. purple,red,yellow,pink and blue are showing on buds and its realy amazing.10/10. Smell are very sweet and strong of grapes.9/10. Taste is very fruity sweet grapes and i like it.9/10. Very strong effect on mind but feel very good.9/10. So, for final, 9/10. Something like week drying and ill update more pics and videos. *Added video of drying buds at day 4. *Added video and pics of well dried buds. ill add more stunning pics soon.

Growing like a champ, lookin like a rich white girl 😂 extremely happy with the progress as this again is my first grow.

The flowers are bigger and denser everyday

happy new year .. wishing you a year full of wealth, health and a kind of happiness and joyful moments that truly matters! happy new year!!