1/12/23 all plants stretching nicely. P4 is vary one sided and P1-3 have similar structures. Starting 12/12 cycle tomorrow, still no pre-flower but should have signs by the end of next week. Hoping for at least 2 oz of or each plant.

Doing some LST and starting next week will be doing some Fimming and the same to the other two. Looking forward to see how big she’s gonna be in a few weeks.

Some leafs are turning yellow which is a signal that the root flushing has worked. I've tried to see the trycomes with s magnifying glass but I don't see any turning ambar yet, the opposite with the pistils, most of them are already brown. At this point I don't know if I should harvest now or wait one more week.

Well, this was a interesting first journey.... the buds finished drying today, 5 days drying, added pics and vids. Just curing to go.... ah nice buds :) Edit: i forgot to mention i went over the buds when i harvested them, and checked / removed any impurities, like gnats caught in the sticky bud.... picked em clean with tweezers... 👍 Edit: needed to air/dry out the buds abit more, and get some Boveda Humidipak for curing.... it's looking good... i'll update with some pics of trimmed, cured buds 😎 The buds turned out beautiful, pungent, pine, berry, citrusy, daytime, medical..... this has theoretical 1:1 - 21% THC, 19% CBD, so it's not going to give u a knockout high.... its medical grade 1:1 ....... side effects - happy, relaxed. haha :) I weighed it dry and came up with about 123.32g (4.35oz) ... i used abit of guesswork in the weight, not to concerned with weight, as to quality.... (that weight is with the test buds added in, +-15g.) .... you can guess the weight from the pics and vids, haha I really enjoyed growing this.... i found the website by looking up the seeds, decided to start a grow diary, it's my first grow, i made alot of mistakes and growdiaries really helped alot, joined a chat group with some other really good growers. Edit - Day 111 - added vid/pic, Been curing for a week, burp and roll em abit twice a day....... smell and taste nicer each day, 👍 Edit - Day 112 - added good vid of trimmed up test bud, beautiful ...... I hope You enjoyed the Diary..... Good Buds 😎

This was round 3 so I had some notes going in for finish time and schedule and have some more after this one for the next. Stay tuned.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Hi liebe Community and Welcome Back! 💚 Woche 8 (Tag 59) im Vergleichsgrow! Bio Tabs NL. vs. Weedefix (Stuttgart/ Germany) Hier im Bio Tabs Grow haben wir nun nach 59 Tagen des Einpflanzens eine Höhe von 118cm erreicht. Blütetag: 14 (BW2) Nach der zweiten Blütewoche, lässt sich eine sehr ähnliche Performance der beiden Düngersysteme beobachten, außer dass bei der Bio Tabs NL Pflanze die Blätter nach wie vor eher etwas dunkler sind. Außerdem hat hier im Bio Tabs NL Grow der Stretch an einem Trieb ziemlich zugelegt. Ein Trieb ist 118cm hoch, während der Rest um 110cm liegt. Es bleibt spannend! Diese Woche, habe ich einmal mit Wasser und 5ml Bio PK 5-8 (Ferment mit Melasse und Bananen extrakt) gegossen. So nach Angaben. Ansonsten nur Wasser. Und es zeichnet sich weiterhin ein stärker werdendes Aroma ab. Die Bedingungen im Growschrank sind für den Anfang in Ordnung, auch wenn es aktuell schwül ist! ————— 🌞 Temp: 26 🌚 Temp: 18°C bis 19°C 💨 RH: 64% VPD: 0,78 kPa 💡ppfd: 630 mpm —————

August 31 - Day 35 Getting there. Everything is basically the same besides the topping I did on August 29th (Day 33). The girls were on their 7th node going on 8 and I topped 3 down to 5 nodes and 1 to 6. Lost at least 5 days to a week in growth on that. But I didn't feel like they were growing enough daily to top them sooner Temp - 78 Humidity - 63 Wattage - 110 EC - 600

April 19th Another 24 litres of nutes at the same strength. Plants are looking healthy healthy and happy. Getting sticky 👌 April 21st Basically just waiting now Plants are doing well, I am going to change up nutrients on the next fill. I will be adding “Dense Buds” 0-0-62 Straight (K) April 24th Changed nutrients today. I have switched to straight “DENSE Buds” 12 litres @ 2 grams per litre PPM =

Starting to see some purple shades

Light = bloomplus bp2500 250watt actuall draw. Exhaust is a ac infinity 6inch with digital controller Inkbird humidity controller with a ram 5l humidifier. Veg only! Soil= biobizz light mix Nutes= biobizz using the 10 week schedule at half dose this run. Heaven =2ml avti vera=2ml Topmax =1ml bloom = 1 calmag=2ml Grow=1ml All nutes mixed to 1l of water Ph= 6.4 Room humidity is 60% Day Temp is 25°c So entering week 3 and the girls are growing fast, gelatocake are putting out pistils to show there sex. The sherbert mimosa are still growing strong and I will bend them and tie them down today. Happy growing guys 👌💪💚

She's had a good week. Roots coming into their own and plenty of lush growth. Topped her this week - bit higher than I usually would - something like the 8th leaf.

i put two autoflowering, i'm out of weed

The girls have filled my space very nicely! i'm heavy handed on the defol trying to optimize bud sites & trying my best to keep everything even as i know these girls will stretch hard! Girls have been enjoying plain water and foliar sprays including EM1

Realicé la que yo creo será la última poda, quedó un tercio de la parte inferior completamente sin brotes ni hojas. Próximamente aplicaré guano de ave y hummus para luego regar de forma alterna con agua y melaza

been really busy.. all is going very well.. got new dairies coming soon from those seedlings🌱 I just started... And 4 Hindu kush will be my first Scrogging... My main plant look unhappy cause he got to settle in New pot