Just one more week for harvest the smell is crazy trichomes milky and amber

Hey Guys, Growth has taken off a lot this past week, have had to add a heater to the grow which isn’t ideal but with warmer months coming this should only be temporary. I have the thermostat set to start the heater when temps go below 23°c. Watching Dr Bruce Bugbee on YouTube, he stated that growth does start to suffer below this temperature. One of the plants has some odd colours and growth which I’m pretty sure is Mg deficiency, which is probably due to that particular girl being more sensitive to PH swings than the rest of the ladies. I have had to insulate some of the circulating pipes as the water cooler was struggling to keep the water baths cool. Seems to have helped keep temps down a couple more degrees, I mate have to reconfigure where my water cooler is in the piping run after this grow Finally a special thanks to the GD team for selecting my previous diary for DOTM, very flattering considering some of the great content that was submitted for March 2020! Happy growing all!

Week3 day4 -Have had the coke bottles how they are as a humidity dome and starting to give a small dose of thrive nutrients

And then there was 3. Placed one bomb in my tent with my Rez in darkness for a few days. Then I will harvest. Other 2 (Charlotte and Bomb) are still going with sensi A and B, bud candy, overdrive. Orange bud is just water now. Will be cutting in a few days Day 102. Cut bomb Finished drying both Charlotte's that were harvested last week. Haven't weighed them yet. But they are both in jars curing.

The Black Runtz scared me to death because one didn't germinate, a big insult for a stubborn person like me. Two days of fear but we already have two plants and maybe a third is on the way. That's why they're still in the biodegradable pot but we're only talking about a few days late, no problem. They're cute and in great shape. For now I've put in fertilizer to root and special enzymes that transform the residues of previous plants into nourishment....perfect I'd say to renew the soil as best as possible. Never stop at the first good results guys, always improve and it's fuel for the next day, to keep the passion alive and have results. Growing Projects -----In this case we were a little late and I planted two plants together. Giving up Main Cola is always a big sacrifice. To avoid depression and to give a better vision to our super trusted breeder, home breeder and Zamnesia heart breeder we make a plant in topping x16 and a straight one preserving the main cola. The first week went only with Power Roots 1 Ml/l and Pure Zym 1ml/l from now on we start with ALga Grow. The plants were transplanted shortly after germination because the biodegradable pots were very small. The soil is Plagron Pro Mix 100% organic renewed with 30% new soil and 20% Perlite added. (Pro mix don't have perlite but is airy we added anyway) Plagron fertilizers are available on Zamnesia in convenient Pack formats for all growing styles. I used this with a simple search you can find the other products. ---- https://www.zamnesia.io/en/12119-plagron-top-grow-box-100-natural.html Try a seed of this strain that drives us crazy... ---- https://www.zamnesia.io/en/11159-zamnesia-seeds-black-runtz.html Zamnesia Description // Black Runtz comes from a truly legendary genetic pool. Our breeders used three modern classics from the United States to create this strain: Zkittlez, Gelato, and Runtz. With 20% THC and flavors of grapes and berries, Zkittlez passed on her delicious flavors and relaxing effects. Gelato contributed to Black Runtz's immense flavor profile by passing on high concentrations of the terpenes limonene and humulene. Finally, Black Runtz inherited the high potency of the original Runtz, a strain with a respectable 21% THC. The end result of this 3-way cross is a strain with 55% indica genetics and 45% sativa. Black Runtz boasts rapid flowering times, high potency, and contrasting yet balanced terpenes that support its delicious flavor profile. Growbox and Air System - Secret Jardin DS120w + fans + Exhaust fans and filters DF16 kit ---- https://www.secretjardin.com All the best that mother nature has to offer is on ----www.zamnesia.com Call for collaboration: I am available for collaborations with companies of Lights and various equipment for growing (No Seeds and Nuts company please) I would like to renovate my 120x120 space that has slightly obsolete lights, and I would like to try some controllers. If you have proposals available.

Tag 49, es geht in die Blüte 😎

Strain: Chill Out OG F4 Day 42 Flower Day: 91 from sprout Medium: Fox Farm Ocean Forest Light: Vivosun VS2000 at 100% VPD: 1.5 kPa Light Distance: 12 inches Watering: By hand, ~oz daily Nutrients: pH Perfect Advanced Nutrition Grow, Bloom, Micro 3 ml / L, 2 ml / L big bud Pistols turning red (20 %) 30 inches

This is about when the last grow died, so i m doing better than ever I plan to keep flowering for a few weeks until i get nice big buds

Day 39-02/09/22 back on track here we go started to give bloom and top max aswell this week!! The one that topped itself is looking good!! One of them does look abit stressed but still to early to tell really!!!

Hace un año exactamente estábamos cruzando uno de estos blíster de dosi- gelato del cual germinaron 3 de 5 semillas regalando nos dos hembras y un macho muy resinoso y oloroso y varias genéticas campeonas y de ediciones limitadas como amerth souer diesel x somango,souer diesel, como le pondría. A ese cruce o a dosi gelato x dosi gelato de hecho esa siempre fue nuestra intención sacar el máximo de semillas posibles de nuestro primer cultivo con semillas importadas primero necesita vamos unas semillas regulares pero de buena calidad y que supiéramos un poco de su genética investigamos acerca de doci-gelato y decidimos que sería la primera base de nuestros cruces sus efectos y aromas nos llamaron la atención aparte de que el nombre estaba de moda abrimos varios blister más como blackberry gum thunder banana moví d y varias que ya no quiero nombrar porque quiero que esos sabores sean invento mío jajajja leeimos un poco de como podíamos hacer semillas y lo hicimos polinizamos 11 hembras originales o estabilizadas que no se escuche feo y 3 hembras regulares una de ellas nos la vendieron con el nombre de Hulk otra como Cherry y la última una buble gum que comparamos su olor y sabor con el de la blackberry gum de seedstockers fue muy parecido a nuestra buble gum pero con notas a moras muy buenas de hecho es un cruce que tenemos contemplado para ver si con una planta macho de buble gum regular y una blackberry gum de seedstockers podemos mejorar aun mas el sabor y el olor en nuestro país de hecho hay buenas cepas regulares hemos probado genéticas con sabor mango,pastel de arándanos,limón ,sandía ,uva y gas,chicle clásico rosa,plátano,zorrillas todas esas genéticas y variedades nos han hecho llegar a pensar que en realidad hay gente que lleva incluso mas tiempo conociendo las semillas importadas o aun mejor importando las para realizar investigar o mejorar las genéticas y sus distintos efectos en el 2012 llegué a acceder a bancos de semillas quedé deslumbrado de la cantidad de nombres que había pero no era 100% seguro entonces no quise seguir insistiendo pero ya se escuchaban nombres como mango kush, Golden Acapulco,cronic el precio era mas elevado que los demas prensados que podiamos conseguir pero eran mejores efectos y aromas ya para 2018,2021 Se empezaron a escuchar nombres como blueberry,amnecia,skunk,pineaple,og,kalifas,jirafa ,puma,gorila,Hulk, chocolopez,candy, arcoiris,cristal,poison,f1,sueño azul , Tutankamón,shark, snow,Jack jerer, y mas llevamos 13 años recolectando semillas de diferentes genéticas de cannabis siempre hemos cultivado esas semillas regulares pero nunca habíamos intentado un cruce para empezar no sabíamos como hacerlo solo sabíamos que no teníamos que dejar crecer el macho. Para 2022 después de un obsequio que tuvimos de un conocido volvimos a intentar una vez más conseguir semillas importadas nos regaló un tubo con 10 semillas del banco sweet seeds con fecha de empaquetado en 2017 llevándonos ala misma pregunta desde cuándo estaban llegando las semillas importadas y entonces algunas veces fumamos de genéticas originales al contrario de sentirme mal por no ser el primero en decir que traje genéticas originales o importadas a mi país me sentí feliz por que sabía que lo que estaba guardando todo el tiempo desde 2012 puede ser una gran colección de diferentes breeders tal vez tengamos varias ediciones especiales como la cepa froot loops, jirafe pussy,cronic,ak47 , y así fue como logramos contactar con un banco que parecía de confianza y obtuvimos dos blíster de doci-gelato , blackberry gum,thunder banana ,banana blaze y varias más hemos crecido varios de nuestros cruces pero necesitamos seguir investigando más aún para lograr estabilisar nuestras propias genéticas les dejo una foto de nuestro cruce María Sabina.

8/4 130am. MONDAY. just AN from now on, no more aminos they will just ad N at this point and counter flowering. 🚨🚨8/4 12:30pm !!! FOR HUMANS!!!!!!🚨🚨👨‍🔬Combining **L-glutamine**, **proline**, **essential amino acids (EAAs)**, **glycine**, and **trimethylglycine (TMG)** in your supplement regimen, as seen in the nutrient mix you described for your cannabis grow, can have significant effects on human health, particularly given your positive experience with methylene blue (“I feel great after I take it”). Since you’re asking about the benefits for yourself (not plants), I’ll focus on how these compounds may contribute to your well-being, their synergistic effects, and how they might align with the energy and mood boost you’ve reported. I’ll also consider the context of your previous questions, including the nitric oxide (NO) precursors and methylene blue, to provide a comprehensive picture. The response will be concise yet thorough, addressing mechanisms, benefits, and potential considerations. ### Overview of Each Component and Their Benefits for Humans 1. **L-Glutamine (500 mg in your mix)**: - **Role**: A conditionally essential amino acid, critical for gut health, immune function, and muscle recovery. - **Benefits**: - **Gut Health**: Supports intestinal lining repair, reducing leaky gut and improving digestion, which can enhance overall well-being and energy. - **Immune Support**: Fuels immune cells (e.g., lymphocytes), boosting resilience, especially during stress or illness. - **Muscle Recovery**: Reduces muscle soreness and supports protein synthesis, beneficial if you exercise or are physically active. - **Brain Health**: Serves as a precursor to neurotransmitters like glutamate, potentially improving focus and mood. - **Relevance to You**: The energy and mood boost you feel may partly stem from glutamine’s role in reducing inflammation and supporting brain function. 2. **Proline (100 mg in your mix)**: - **Role**: A non-essential amino acid involved in collagen synthesis, tissue repair, and antioxidant defense. - **Benefits**: - **Joint and Skin Health**: Promotes collagen production, supporting joint flexibility, skin elasticity, and connective tissue repair. - **Cardiovascular Health**: Supports arterial wall integrity, potentially complementing the NO precursors in your mix (L-arginine, L-citrulline). - **Stress Resistance**: Acts as an osmoprotectant, helping cells manage oxidative stress, which may contribute to your “feeling great.” - **Relevance to You**: Proline’s low dose may subtly support tissue health and stress resilience, enhancing your overall vitality. 3. **Essential Amino Acids (EAAs, 300 mg in your mix)**: - **Role**: EAAs (e.g., leucine, lysine, methionine) are amino acids the body cannot synthesize, essential for protein synthesis and metabolic functions. - **Benefits**: - **Muscle Maintenance**: Stimulates muscle protein synthesis, aiding recovery and preventing muscle loss, especially during exercise or aging. - **Energy and Mood**: EAAs like leucine support energy metabolism, while tryptophan and phenylalanine are precursors to serotonin and dopamine, potentially boosting mood. - **Metabolic Health**: Supports insulin sensitivity and nutrient metabolism, contributing to sustained energy. - **Relevance to You**: EAAs may enhance your energy and mood by supporting muscle recovery and neurotransmitter production, aligning with your positive response to the mix. 4. **Glycine (1 g in your mix)**: - **Role**: A non-essential amino acid involved in collagen synthesis, neurotransmitter regulation, and detoxification. - **Benefits**: - **Sleep and Mood**: Acts as an inhibitory neurotransmitter, promoting relaxation and improving sleep quality, which can enhance mood and energy. - **Cognitive Health**: Supports NMDA receptor function, potentially improving memory and focus. - **Collagen and Joint Health**: Like proline, supports connective tissue and skin health. - **Detoxification**: Aids in glutathione production, reducing oxidative stress and supporting liver function. - **Relevance to You**: Glycine’s calming effects and antioxidant support may contribute significantly to your “feeling great,” especially if you experience better sleep or reduced stress. 5. **Trimethylglycine (TMG, 1 g in your mix)**: - **Role**: Also known as betaine, TMG is a methyl donor involved in homocysteine metabolism and cellular protection. - **Benefits**: - **Cardiovascular Health**: Lowers homocysteine levels, reducing heart disease risk, and may synergize with NO precursors for better blood flow. - **Exercise Performance**: Enhances hydration and muscle endurance, potentially improving physical energy. - **Liver Health**: Supports fat metabolism and detoxification, reducing liver stress. - **Mood and Stress**: Methylation supports neurotransmitter synthesis, potentially boosting mood. - **Relevance to You**: TMG’s role in energy metabolism and methylation may amplify the mood and energy benefits you’re experiencing. ### Synergistic Benefits of the Combination The combination of L-glutamine, proline, EAAs, glycine, and TMG, alongside the NO precursors (L-arginine, L-citrulline, etc.) and methylene blue from your mix, creates a synergistic effect that likely explains why you “feel great”: 1. **Enhanced Energy and Recovery**: - **Glutamine, EAAs, and TMG**: Support muscle protein synthesis, energy metabolism, and recovery, reducing fatigue and boosting physical vitality. Beta-alanine (from the NO capsule) buffers lactic acid, enhancing exercise endurance. - **NO Precursors**: L-arginine and L-citrulline increase NO production, improving blood flow and oxygen delivery to muscles and brain, enhancing energy and focus. - **Methylene Blue**: As you noted, methylene blue enhances mitochondrial efficiency, boosting ATP production, which complements the energy-supporting effects of glutamine, EAAs, and TMG. 2. **Mood and Cognitive Boost**: - **Glycine and EAAs**: Glycine promotes relaxation and sleep, while EAAs (e.g., tryptophan, phenylalanine) support serotonin and dopamine production, enhancing mood and mental clarity. - **TMG**: As a methyl donor, supports neurotransmitter synthesis and reduces homocysteine, which is linked to mood disorders. - **Methylene Blue**: Its mild MAO inhibition may increase serotonin and dopamine levels, amplifying the mood benefits you’re experiencing. 3. **Stress Reduction and Resilience**: - **Proline, Glutamine, and Glycine**: Act as antioxidants and osmoprotectants, reducing oxidative stress and supporting cellular health under physical or mental stress. - **TMG**: Enhances methylation, supporting stress resilience and detoxification. - **Methylene Blue**: Its antioxidant properties may reduce cellular stress, complementing the other compounds. 4. **Cardiovascular and Tissue Health**: - **Proline, Glycine, and TMG**: Support collagen synthesis and cardiovascular health, improving joint, skin, and arterial function. - **NO Precursors**: Enhance vasodilation, improving blood flow and nutrient delivery, which may contribute to your overall sense of well-being. ### Why You Feel Great Your positive response (“I feel great”) likely stems from the combined effects of: - **Mitochondrial and Energy Boost**: Methylene blue and glutamine enhance ATP production and energy metabolism, giving you a physical and mental lift. - **Neurotransmitter Support**: Glycine, EAAs, TMG, and methylene blue support serotonin, dopamine, and GABA activity, improving mood, focus, and relaxation. - **Reduced Stress**: Proline, glutamine, and TMG combat oxidative stress and support recovery, reducing fatigue and enhancing resilience. - **Improved Blood Flow**: NO precursors increase circulation, delivering more oxygen and nutrients, which may enhance energy and cognitive clarity. The doses in your mix (500 mg glutamine, 100 mg proline, 300 mg EAAs, 1 g glycine, 1 g TMG) are within typical supplemental ranges, suggesting a balanced approach that avoids overload while providing cumulative benefits. ### Considerations and Precautions 1. **Dosage Safety**: - Your doses are moderate and generally safe for most adults, but long-term high doses of TMG (1 g daily) or glutamine (500 mg daily) may stress the liver or kidneys in sensitive individuals. Typical supplemental doses are 500–3,000 mg for TMG and 5–10 g for glutamine, so you’re on the lower end. - Methylene blue (2 drops of 5% solution ≈ 5 mg) is a low dose, but its long-term use lacks extensive human studies. Monitor for side effects like nausea or dizziness. 2. **Interactions**: - **Methylene Blue and SSRIs**: Methylene blue is a mild MAO inhibitor and can cause serotonin syndrome if combined with SSRIs or other serotonergic drugs. Consult a doctor if you’re on such medications. - **NO Precursors**: L-arginine and L-citrulline may lower blood pressure, so monitor if you have hypotension or take related medications. - **TMG and Methylation**: High TMG doses may overstimulate methylation, potentially causing anxiety in some individuals. Your 1 g dose is moderate but worth monitoring. 3. **Individual Response**: - Your positive response suggests your body responds well to this mix, possibly due to baseline deficiencies (e.g., in glycine or glutamine) or high metabolic demands (e.g., from exercise or stress). Genetic factors affecting methylation (e.g., MTHFR mutations) may make TMG particularly effective for you. 4. **Nutrient Timing**: - Taking this mix in the morning (as implied by 2:47 AM) aligns with boosting energy and focus for the day. However, glycine’s calming effects might be better utilized at night for sleep support. Consider splitting doses (e.g., glycine in the evening, others in the morning). ### Recommendations - **Optimize Timing**: Take glutamine, EAAs, and TMG in the morning for energy and recovery, and glycine in the evening for relaxation and sleep. Proline can be taken anytime due to its structural role. - **Monitor Effects**: Track specific benefits (e.g., energy, mood, sleep, exercise recovery) to identify which compounds drive your “feeling great.” If methylene blue is key, its mitochondrial and mood effects may be central. - **Hydration and Diet**: Ensure adequate hydration and a balanced diet, as amino acids and TMG increase metabolic demands. Pair with B vitamins (especially B6, B12, folate) to support methylation and amino acid metabolism. - **Consult a Professional**: Discuss this regimen with a doctor or nutritionist, especially regarding methylene blue and NO precursors, to rule out interactions or contraindications (e.g., G6PD deficiency for methylene blue). - **Adjust Doses if Needed**: If you notice side effects (e.g., jitteriness from TMG, GI upset from glutamine), reduce doses (e.g., 500 mg TMG, 250 mg glutamine) and reassess. ### Conclusion The combination of L-glutamine, proline, EAAs, glycine, and TMG likely contributes to your feeling great by boosting energy (via mitochondrial support and muscle recovery), enhancing mood (through neurotransmitter production), reducing stress (via antioxidant and osmoprotectant effects), and supporting cardiovascular and tissue health. Methylene blue and NO precursors from your mix amplify these benefits by improving mitochondrial function and blood flow. Your moderate doses are generally safe, but careful monitoring and medical consultation are advised, especially for methylene blue. If you’d like, I can search for recent studies or X posts on these supplements for human use, or tailor a dosing schedule based on your goals (e.g., energy, mood, recovery). Let me know how you’d like to proceed!🚨👨‍🔬. 8/4 1230pm. 8/5 12:50am HEY HARRISON SMITH, HARVEY WALL BANGER, GET IT?...IM SO GOOD. ANYHOW....FLUSH TODAY, FLORAKLEEN 5ML PER GALLON ALL OF THEM..... NO DONT PILLAGE ME AND IM LIKE NO! IM PILLAGING EVERYBODY, YOU INCLUDED. 8/5 3:51 AM I mixed up floor clean 5 mL per gallon doing bubbles first and then goats and the other girl next to it👍 8/5 7:10 AM I’ve been doing this since five and I can’t believe how high my EC was. It was off the chart like 6000 7000 so I had to do the flush. I’m never really bothered to do this before. I’m sure it’s gonna be a lot better. One of my last grows. The leaves were so full of garbage. Basically my weed smell like a hamburger so not exactly good but I’ll save it for people. I don’t like👍. Sometimes it doesn’t remember a whole paragraph. I just fucking typed piece of shit took me six hours to do four fucking plants.🤣💥💥💥 8/6 408am. The colas on the oldest of the 3 are AMAZING!!!! they are all small but MIGHTY!! 2 cola grows in 1 gallon seem to be about right. I will probably get 4oz dry from EACH!! That is really acceptable!!! For a home micro- grow where the goal is ALSO to grow as many different kinds, to get more seeds, which keeps the seed companies coming up with new strains!!!! The 1 gallon plastic pots are light, small bags of Soil Sunrise from online is easy to store, CLEAN! the bags of dirt it comes in are strong, and easy to wipe up when done. The 8qt fills several pots I also mix in about 4 8oz cups of Perlite per container to stretch the dirt out a bit. Soil Sunrise delivered by amazon is the only dirt that did NOT have bugs...I used to order HUGE bags of FF delivered from outdoor garden centers all over the country ...the bug infested HORROR, never again!!! 8/6 418am Also my body is starting to fail, I can feel it. If there is any discomfort there will be no more updates. thats life. doesnt it go by in a blink? I think to myself what a bullshit goblin & demon infested shithole of a world, cant dies soon enough. And death to the shitters of 1978!!!😡 Did you ever hear about the Tragedy of Darth Arnie and Christine? He's more Chrysler 1958 Plymouth Fury than man now...maybe I got the movies mixed up again....idk....

What a stretch from the last week, week and a half... Both of the gelato and forbidden runtz are 3 feet tall!! The vigor and structure look great, I let these 2 go as a normal plant with no training and they have proven themselves to be great plants.

End of week 10 and so close to harvest! I have finally decided to chop the Purple Lemonade because she has been ready for a few days now literally oozing out sweet sticky sugars and the trichomes were about 70/30 cloudy to amber ratio. The other plants are about 90/10 and will be chopping the ladies soon. Smoothest grow I’ve had. Also only the 2nd (Indoor) grow. It’s nice to see some progress and will always try pushing to grow better and frostier flowers. One love!

i feed with nutes last watering about two days ago my idea is to feed one more time before the two week flush not really sure yet i have at lease a week to figure it out being the end of the plants life cycle is soposed to end in another week idk master growers were yall at?????😅

10 weeks from seed to harvest. The nutrient line I was using didn’t give me optimal growth, for this strain and my level of experience. Had some great growth and veg time, about 4 weeks, but when flower kicked in they got beastly. I would say I had two different phenos of this strain, and the one pheno that is unique, had slightly looser buds than the other two. This could be because of the LST/super cropping of the main cola. The other two had nice rock hard frosty buds and have similar smells and profile. The other one has more citrus, and was a bit lanky, more sativa in looks. Overall, easy enough to grow, no major surprises, and decent enough yield for 10 weeks. This is a strain that could have been better had I not had some issues with either lockout/deficiencies.

In this week I decide to top her to break the apical dominance and start training her on the next weeks,let's keep on working and growing,enjoy life growmies!! 💚 🌱 ❤️💛

****She's now Eleven weeks old, I water her 5L a day with with 5ml/L Terra Bloom . The co2 will stay 1600 ppm .Week 12 starts (2/02/2025) tomorrow .Im still aint using a heater on my attic so Im now using less electricity then my growths. She growth again approx 4cm in bud height in one week so im happy .Wish you all a nice Sunday and hope that you will have a nice week a front of you ! 🙏😘♥️💚♥️💚♥️ ** Hello everyone! I am proud to announce that I have been asked to grow a seed of choice from Zamnesia. My choice fell on the Tropicana Cookies Autoflower (Zamnesia Seeds) feminized strain. Strain info https://www.zamnesia.com/10922-zamnesia-seeds-tropicana-cookies-automatic.html I hope you will enjoy this new grow just like I will do. Let's set another record for this strain, just like with the previous strain.👌 ENVIROMENT: **It took her seven days to poke her head above the ground. It's probably my fault, because I was impatient and planted her too soon, as she hadn't even reached half an inch of root. All in all, she looks healthy, and I'm happy with the result. I'll be more patient in the future, but I was too enthusiastic to start a new diary after a five-month absence from GD 😅😅** Light schedule : 24/0 Humidity : 50% Soilmoisture : 42% Temp : 26 Celsius Who am i ? I have always grown hydro for a little over 11 years. But growing started to look more and more like work because i was more busy with programming my controllers for the ph / humidity / lights / co2 / temperature. Also the realization that i have 2 children and my own house made me nervous for the authorities because where i live growing is not allowed. So i decided to grow smaller where i previously had 200x180x200 space i opted for 70x70x160 with the result that i can only grow 1 strain at a time. The intention is to remain self-sufficient in this way and still be able to exchange or give away something here and there at a party. Although I am sponsored I will not be able to grow every strain because not every strain can deliver the result I need to remain self-sufficient. If you know of an auto strain or photo strain that takes at least 11 to 15 weeks I would like to know so that I can grow it in the future and of course I will try to set a record for that grown strain. Because during the 4 grows that I can do per year I can only try to achieve a record since I cannot show you multiple strains at once. I am happy that after 11 years I have found growdiaries and that I have met you my friends.Greetings MonyetDiablero.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. 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