CalGonJim 4/28 330AM MONDAY LAST night I replanted....I lost the seed, maybe it ran away. 4/29 3pm looking great, waiting for the other seed to wake up, or the one I lost to sprout somewhere else? 4/30 6am red blue light 24 hrs. main light 12. now to 18 hours 3 setting. Great results 👍👍👍 5/1 1230am NO GO, 2nd not to germinate. Cant find that one either....... SO for the FOURTH time, Im throwing the seed right in the dirt again, if it doesnt work..f it I will cancel this grow and throw them all away. 130 am found it. 💀 dead probably gonna cancel this girl. I wouldn’t follow this anymore. Maybe I’ll move the plant to the other one.☠️. 6am Dug it up, put it in CannaKan with a new seed, THEN the tail popped out so I planted her AGAIN and I have a new seed soaking now. #4 is soaking, dont know if 3 will work pretty cool looking seeds. 5/2 12am #4 is good, unless I forgot to change the water an hydrogen peroxide kills the tail. going to let it go until I see if #3 survived. 245am she's alive I brought her back from the dead. I dug her up fixed her, put her back in the ground and now she’s coming back to life. I can do that with insects mostly just bees though.👍👍👍👍👍👍🚨🚨🚨🚨. 1230pm 3 Bubbles now growing, I still can NOT figure out where the other seed went..... I really LOST a seed.....it germinated, I put it in the dirt...maybe I couldn't see and I flung it out some how....SOMEWHERE A BUBBLES SEED IS IN THE HOUSE....ITS ALIVE.....I CANT FIND IT..... IT MIGHT TURN INTO A CONKEY.

CalGonJim 5/5 MONDAY Took out the AC Infinity lights and just went with the 2 cheap blue and red lights. Samsung 301B.. H?....great lights but so powerful and no blue red combo. I noticed the plant grow towards the red spectrum LED than the whiter ones on the AC system. There are 2 on each bar. I use the AC lights as auxiliary when I get need it for taller plants with lots of vegetation. 2pm looking great. '"Sir, I have seen nae deerskin treaty, nor have I seen fairies or water sprites. But what I do have are Malay coins, enough to bury you. Prayer beads, not enough to get you to heaven. And hashish, enough to ease my grieving when the East India Company slit your throat, which, of course, they will." ― Brace in a conversation with James. 5/5 11pm Monday night, MORNING for me.....WHAT IS THIS????? CONKEY IS ALIVE!!!!!! CONKEY IS ALIVE!!!!!! CONKEY IS ALIVE!!!!!! CONKEY IS ALIVE!!!!!! CONKEY IS ALIVE!!!!!! HOLDY SHILT I CANT BELIVE IT, NOW WHAT THE FUCK DO I DO???? I GOT OUTSMARTED BY A SEED!!!!!! A-FUCKING-GAIN!!!! HOW CAN I NOT BE SMARTER THAN A SEED....??????🚨 5/6 1230am..... ALL OF THIS IS SOMEONE ELSE FAULT....... Now I have a freakshow Siamese twin plant, PERFECT. When Im not flying off in a rage about things I break....it's a fucking comet of disaster.... I typed comedy you stupid ficler.... Maybe I keep them in the same pot and call them Cory & Trevor!!! 2am They are getting a little humid so I have to open the top for a while. The cheaper lights work better. and are less than 15w 5/7👍🚨Looks like Cory & Trevor are fighting 😂😂 5/8 looks good. 8am getting great photos on small iPhone by pulling back a little bit so it doesn’t focus too far behind. 5/10 2am. I was able to empty my disgusting body of toxic shit...these plants are rebuilding by digestive tract and systems. A multi-year painful endeavor I have achieved through weed. so fuck you if you dont like it. I will defend my rights with overwhelming force. if you try and take it. you know exactly what I mean and yes this means you badgey mc fafo. 5/11 12 pm

CalGonJim 5/12 Monday 12 am. They are looking great now. Fuckn' Randy forgot to open the vents on the humidity dome and it made the plants all retarded. HEY HEY IM FUCKING SMART..... REaDY???? 🚨 TRAILER PARK BOYS HUMIDITY DOME. Make a trailer looking humidity dome all themed out, part of a kit. great idea. PLANT DAUGHTERS DONT GET TATOOS....I LOVE THAT ABOUT PLANTS 5/13 8 am 5/14 1am looking great. I have to separate Cory & Trevor today.....they fuck up everything ...FUCKN RANDY!! 11am 5/15 2am looking great. 3pm. water 2ml per L+10 Con Grow

CalGonJim MONDAY 5/19 12AM Doing great, have to separate Cory and Trevor this week. 1130 pm .🚨🚨🚨.STONED GENIE order..I got one broken 64 dollar land the 12 inch 19 dollar black double percolator was TOTAL GARBAGE!!!!! it had a fake 10 star review.... at least I got 2 4 inch down stems for 117 .....complete WASTE of money, should have just thrown the money in the garbage.......... so pissed....🚨🚨🚨 5/24 3 pm new light lots of godamned work why do I do this to myself??? 330pm what. a nightmare, I put the extra red and blue 20 cheap lights with this monster light, nice balance of color with the blue. I love this light, Mars Hydro...why do you do this to me. Totally worth it, best lights on the planet, so far. 5/25 230am NEW DESIGN NIGHT. USB FANS -- Small usb fans at the end of ducting are effective intake air filters. HOWEVER the fans are cumbersome the wires are a pan in the as. design 2 part clip gravity 4 inch wire. twists thru fan . ONE tlong up the tube for balance other to CLIP on to duct. 3am clips are good new designs emerging. fasting all night so I can work and get new ideas. fans are a go, we have a new design using clips, no external wires. the load bearing usb wire is safe, not load bearing to the connections just the cable, its perfect.. Mexicans and jew & white trash cops and their arzonz fed family and judges have been committing unimaginable property and financial crimes against me. If I am suicided. it was them. I am ready to die, unafraid of every one of you fucking bastard traitors and I mean it.

CalGonJim 5/26 MONDAY 4am. moved into Tent 1 with NEW Mars Hydro Light!!!!! had it for about a year, finally get to use it!!! 5/273pm. 1130pm if I had a magnetic printer ink to print the 4 inch O Rings on vinyl sticker paper around the ducting it would make for cheap easy lightweight magnetic connectors for a kit. 5/29 144am doing swag stuff. then a directed energy attack.....ringing ears piercing high pitched noise like original Superman movie 5/30. FAN INFO. D4 VIVOSUN FAN I REMOVED inner hand made filter for dust . Restrictive ALSO I think it adds to power use...do a test with filter on and off find out watt difference. 6/1 212am Sunnyvale wiped out by Predator vs Alien fight no survivors ............ Alien chest explosion ...Im all better now. ... Time to separate Cory and Trevor....lazy bastards. 1123am time to separate Trevor and Cory .....we can rebuild them....The Bionic Cory and Trevor....coming soon. FUKN RANDY screwed it up. Cory and Trevor might not survive.... WELL, WE KNOW WHAT DOESNT WORK.... My experiment ripping the root ball in 2/3's caused Cory to explode and he's dead.... Im gonna need a new Cory.

CalGonJim 6/2 MONDAY 1AM. CORY IS ALIVE!!!!!! EVERYONE MADE IT. I split up a GOAT'lato and they both lived too!!!!! My stupid experiment ripping 2 plants accidentally planted in the same Bud Cups worked!!!! 6/3 430am The Ricky plants were junk. shitty spindly little branches that never got thick enough to support or grow a good amount of weed from. I have that GOAT'lato that was mislabeled from RQS, they are NOT autos. I will separate all the "BUBBLES" AND VEG them for at least 3 months I cant waste my time on plants that can not grow in my timeframe or environment!!!! So I have a feeling this will be a fail too. 6/4 444am If Tatooine had not allowed so many wizards to immigrate there none of this would have happened. Import the Wizard World, Become the Wizard World 6/7 they are vegging just nicely. I’m gonna take the biggest one top her and then I will put her to flower. I’m gonna take a branch off of Ricky and I’m going to re-veg that one and grow both of them together.

CalGonJim 6/9 315am 🌿👍🌿🤨🌿🌿👍 TOP this week. IDEA Sinatra sings Stefani...... Im Just A Girl, or Dont Speak whichever is funnier to the melody of Fly Me To The Moon....comedy gold. 6/10 11 pm top soon 6/12 9am I want the mainstalk to be bigger before I top this time. 🚨MY ORANGE PH PEN WAS OFF 2 POINTS ALMOST I FINALLY FIGURED OUT HOW TO CALIBRATE....DAMN IM RETARDED.... I FIXED ALL MY PH PROBLEMS AND ALL OTHER PROBLEMS VANASHED🚨. I got a replacement bong sent from Stoned Genie, it's really nice. 6/13 1221am. Bram Stokers Trailer Park Dracula. Bubbles "Boob Hair" Dracula, Julian - Jonathan Harker, Ricky -Reinfeld, or Ricky is Red Armor Dracula, cause he's pissed he wants the cool armor, but it's all broken. Randy Van Helsing. Sara - Mina, fell out of the trailer & faked her death for the insurance money... but they dont know what insurance is, so they dont know you have to buy it first. Julian has been running a Dracula scam. 6/15 736am. Faces come out of the rain whether you are strange or not, really stupid.

CalGonJim 6/16 12am MONDAY MEESA TAKEN BLUEBERRY CLONES TODAY!!!!!!!!! I only got one clone, I already have enough going. I got 3 Ricky's in flower. I have 4 Gorilla Gas F1 Fast clones for some reason. I'll be doing a Bubbles, Ricky get Gorilla Gas Clone grow next. 420 421 Whatever it takes....Im number 421! 6/19 Sinatra sings the 90's When Guinea met Grunge ....... classic.. 8am losing weight fast, tied. ready to go.. 6/21 253am GOT SOME MORE GROW STUFF!!! DOING HERBS AND FOOD NOW AND BUTTERFLYS, MANTIS AND LADYBUGS. I MADE HABITATS FROM 12 AND 14 INCH HEAVY DUTY PLANT TRAYS AND A "4 Pcs Pop Up Insect Barrier Plant Cover Net, 19.6" x 11.8" Round Foldable Plant Protectors Mesh Plant Enclosure"!!!!!!! MY WHOLE LIFE I WANTED AN INDOOR FANCY GARDEN JUST LKE ON ADDAMS FAMILY TV SHOW, NOW IM DOIING IT!!!

CalGonJim 6/23 4am MONDAY Bubbles looking good. "Randy the Fag" is in flower with a Gorilla Gas F1 the rest still in veg for another month until I see a much thicker main stem 6/25 1am I cant get mail or travel without getting some kind of problems with Mexicans and low rent Christian midwits, haven't felt I lived in a freee country in decades, Whites rounded up by black sheet dictators to play revolving door monitor to bottom feeding trash attacking old Whites every day...but thank god though we are attacking everything a dirty cop from the 90's tells you is scary. 6/27 353am.....Will Methylene Blue give superpowers to my plants, or destroy them.....WE ARE GOING TO FIND OUT, IM ABOUT TO GO FUCK AROUND!!!!! They are not dead yet, I wanted WAY YOO LONG to transfer they will be small but it's fine I can grow more. This is my FIRST grow in Vita wool. 2 drops of methylene blue in a liter with nutes..... 6/28 3am NOBODY DIED FROM THE MB. The one in Flower looks great. The others are still in veg. Im doing a parallel Gorilla Gas F1 by Seedsman. Bud Ignitor didn't have much of an effect on BB, AN nutes are still great. RAPID START .5ml per liter has shown me bigger leaf and healthier leaves than just AN grow alone . Mythelne Blue had no negative effects, poured small amount in on place. nothing.

CalGonJim 6/31 2am Bubbles are all doing great, I have 2 extras so I dont know what to do with them I will try low power and keep to for a few months then flower th other 2 7/2 1130pm I have the props to do a dramatic Last Samurai ending when I chop the plants....I plan on making it as disturbing as possible. the Colas can have little Cobra Kai head bands 7/4 445am five drops of quick sorb amino acid into 2 L of water with one drop of methylene blue in 2 L of water 4 mL per liter advanced nutrients connoisseur bloom pH 5.7👍👍🚨🚨👍👍👍👍👍. 11:19am I turned the light up to 75 W. I was having great results at 59 W for vegging. I may be able to grow these three plants at 75 W. That would be amazing.👍👍 7/6 745am nitric oxide, boost and quick sorb amino acid experiment successful. I am able to keep the lights at a lower wattage now that I am able to use special amino acids that increase photosynthesis so I’m using amino acids along with my entire other system to keep the wattage down isn’t that fucking amazing yes, it is. You have no idea. 🚨🚨🚨🚨🚨🚨🚨. 330pm Great results today.

CalGonJim 7/7 320am WOW!!!!! AMINO EXPERIMENTS USING PLANTS UNDER EXTREME STRESS COMPLETE SUCCESS. NOW TO FIND AMINO FORMULA AND REPEAT FINDINGS.... 1 PLANT HAS NOT ENOUGH SOIL AND WAS RIPPPED!!!! IN HALF DURING TRANSPLANT!!! NO BURNED TIPS..HOW IS THAT POSSIBLE???!!! IM SOFA KING AWESOME 7/8 3am 7/9 520am.......so very talented. 7/10 11am 8ml A 8ml B Con., 3ml Bud Ignitor (GOATS & BBBf4) Big Bud 3ml (GC Auto, G Gas F1, BBB f4) 10 drops Quick Sorb Amino Acid, Methylene Blue 1 drop, 1 Nitric Boost emptied capsule into water. 145pm... They look great!!!! Bright green branches....so full just perfect. See my parallel grow for amino info. 7/11 124am. Reviwing pictures on the big screen....I can visually analyze on the TV better than Bladerunner....so cool. GREEN VIBRANCY ACROSS ALL PLANTS GROWING AT THIS TIME WITH NITRIC BOOST, AMINOS AND MYTHELYNEY BLUE. RHINO SILICA .25ML PER L STALKS BRANCHES STAY BRIGHT GREEN LONGER AND ARE STRONG STRONG STRONG!!! TODAY TRIM AND DEFOLIATE THEY ARE SO HUGE. 7/11 130AM MIKE TYSON 2.0 GOATS are still like nothing Ive seen!!!!!!!....I ALSO have G Gas F1 AND Bubbles Blueberry F4 clones as testers. I have poured FULL Strength everything and they are ROCK SOLID UNAFFECTED, UNBURNED!!!! 🚨🚨⚡️⚡️💎💎💎🚨🚨🚨⚡️⚡️💎💎💎🚨TOTAL SUCCESS!!!! NITRIC BOOST 1 pill per liter or 2 liter, 5 drops or more per L Quick Sorb by Biotics Research AMINOS, METHYLENE BLUE 5 percent 2 drops per L during veg, 1 drop during flower. .25ML (one quarter) PER L RHINO SILICA. Advanced Nutrients as a base. Bud Ignitor when appropriate pre flower time, then 1/2 strength BiG BUD at flower!!!! THAT IS THE SECRET FORMULA FOR GOD MODE!!!!! 🚨🚨⚡️⚡️💎💎💎🚨🚨🚨⚡️⚡️💎💎💎🚨 7/12 641am I watered all four of the bubbles plants in 10 three one of them has been in flour for two weeks longer so I can see the difference. I added a different product today Bloom tastic as a supplement. They are all doing exceptionally well, I did methylene blue and amino acids and nitric boost for extra amino acids along with advanced nutrients connoisseur for milliliters per liter like it says on the bottle all the other plants look really good👍👍👍🌿🌿. 715am Turned Lights Up to 98.8 from 93.3 , 89w 7/13 330am All experiments are going very well. The Tammy experiment gave us a little burn tip because it’s incredibly powerful even at 1 mL per liter. That’s a maximum I could use. They are also strong and doing so good the methylene blue the amino acids the enzymes just fantastic even on the clone. 👍👍👍👍🌿🌿. 7/13 140pm So tired all of a sudden cant hardly move and sudden hypothermia can set in. I risk injury if I keep trying to move around once the brain and body break down in the afternoons. Awesomeness levels are still high, my God like ability to overcome arrogance AND modesty all at once is absolutely perfect, probably the L-Amino's and smoking plants with blue dye and experimental amino acids no big deal.

CalGonJim 7/14 4AM MONDAY MUST DEFOLIATE!!!!! I HAVE 4 INSTEAD OF 2...BECAUSE OF MY LITTLE ACCIDENT EARLY ON.. I WILL TAKE ONE OUT OF FLOWER AND RE-VEG TODAY!!! 7/15 420am They are great cutting I took last month or week is great. Amino and mb today!! 230pm spectacular results today heavy defoliation I removed branches. I prefer a four cola grow in small spaces. It’s just better for airflow better for the plants and they responded so great. It’s like they’re jumping straight up now. I’ve never seen this. They’re just so vibrant that means all the amino’s. Everything is working perfectly. 👍👍👍🚨🚨🚨🌿🌿🌿 7/16 320am 🌿🌿🌿🌿🎊🎊🎊🎊💥💥💥💥👍👍👍👍 7/18 3am NOTICING Elongated Sepals!!!! from Search Ai "Elongated sepals in plants refer to sepals that are longer than average, often appearing narrow and pointed. This characteristic can be a defining feature of certain plant species, such as the long-sepal beardtongue (Penstemon calycosus). The length and shape of sepals can vary significantly between species and even within the same plant, influencing the overall appearance and potentially the function of the flower. Elongated Sepals: More Details Definition: Sepals are leaf-like structures that form the outermost whorl of a flower, typically green and protecting the bud. Elongated sepals are simply those that are noticeably longer than the typical sepal length for a given plant or group of plants. Examples: Long-sepal beardtongue (Penstemon calycosus): This plant is named for its elongated sepals, which are a key characteristic. Berberis (Barberry): Some Berberis species exhibit elongated sepals, with variations in shape and length among different sepal types within the same flower. Significance: Identification: Sepal shape and length are important features used in plant identification and classification. Pollination: In some cases, elongated sepals might play a role in attracting pollinators or influencing how they interact with the flower. Protection: Sepals, even elongated ones, still function to protect the developing flower bud. Morphogenesis: Studies on Arabidopsis have shown that the shape of sepals, including the presence of elongated tips, can be influenced by mechanical feedback within the plant and the arrangement of microtubules."""🚨. THIS IS ON 2 OF THEM, SO I MAY HAVE 2 STRONGER THAN AVG. PLANTS. I WONDER IF THE MB, AMINOS ARE HELPING WITH THIS TOO??? 7/19 I GOT A MAGNETIC STIR THING. 150pm they didn’t need a lot of attention today. They do need to be trimmed back down to four colas. I’m running out of space they are very perfect and if I had more space the leafs would be bigger, but that’s limited by mine condition so there’s nothing I can do but they are absolutely incredible🎊👍👍

CalGonJim 7/21 MONDAY 330am 7/22 11am I am coming up with the sickest movie villan stupidest grow themes...... RACE WAR GROW!!!!! WHITE OG VS DARK DEVIL AUTO!!...something like that... SwastiPot Grow Stuff from the move Top Secret. 6 PLANTS NO WAY, 2 OR 3 AT BEST. k..omg. KUSH KLUX KLAN!!!!! PUT LITTLE HOODS OVER THE COLAS.....GENIUS!!! 7/23 4am adding TMG powder to the mix. The really helps a lot. I was surprised it works good.👍👍. 817am great news!!!!! CYSTS ARE SHRIKING, I AM CURING THE CYST IN NON-CONVENTINAL WAYS AND IT WORKS. INTERNAL ORGANS, INTESTINES, LOWER AND UPPER LARGE AND ZLINE FROM STOMACH ARE HEALED. NO SWOLLEN ORGANS, BACK TO NAORMAL SIZE!!!!! LIVER FUNCTIONING, SKIN IS NORMAL, TEMP NORMAL. 7/23 2am * Glycine: Supports chlorophyll synthesis and nitrogen metabolism, potentially enhancing photosynthesis and growth. * L-Alanine: Involved in energy production and nitrogen assimilation, which could promote vegetative growth. * L-Arginine: Can enhance root development and stress tolerance by supporting hormone production. * L-Lysine: May contribute to stress tolerance through metabolic pathways, though it’s less abundant in plants naturally. * L-Proline: Known for improving stress resistance (e.g., drought, salinity) by stabilizing cell membranes and acting as an osmoprotectant. * L-Histidine: Supports enzyme activity and stress responses. * L-Serine: Contributes to protein synthesis and cell membrane formation, potentially aiding growth. * L-Threonine: Supports immune function and protein synthesis, which could enhance overall plant vigor. * L-Valine: A branched-chain amino acid that may support energy provision and growth under stress. added 7/24 2am --- Leucine, an essential amino acid, plays multiple roles in plants, including regulating translation, influencing stress responses, and contributing to overall plant health 7/25 845am Could you tell me the effects of adding a nitric oxide pill which contains arginine 400 mg, arginine AKG 2.1 extract 400 mg, L citrulline HCL 200 mg, L citrulline melate extract 200 mg,and betaine 60 mg along with an 8th teaspoon of TMG powder and five drops of quick sorb amino acid and the normal dosage of advanced nutrients, connoisseur bloom and 1 mL of a ATAMI Bloomtastic will have on my marijuana plants? The combination of supplements and nutrients you’ve listed—nitric oxide pill (containing L-arginine, arginine AKG, L-citrulline HCl, L-citrulline malate, and betaine), TMG (trimethylglycine) powder, quick sorb amino acid, Advanced Nutrients, Connoisseur Bloom, and ATAMI Bloombastic—introduces a complex mix of compounds to your marijuana plants. Below, I’ll evaluate the potential effects of each component on cannabis growth, focusing on their roles in plant physiology, while noting that direct research on some of these supplements in cannabis is limited or nonexistent. I’ll also address potential risks and considerations, as the use of these compounds, especially in combination, is unconventional for plant cultivation. ### 1. **Nitric Oxide Pill Components** The nitric oxide pill contains L-arginine (400 mg), arginine alpha-ketoglutarate (AKG, 400 mg), L-citrulline HCl (200 mg), L-citrulline malate (200 mg), and betaine (60 mg). These are primarily designed for human consumption to boost nitric oxide (NO) production, which enhances blood flow and nutrient delivery in animals. In plants, nitric oxide plays a different but significant role, acting as a signaling molecule that influences growth, stress response, and nutrient uptake. - **L-Arginine (400 mg) and Arginine AKG (400 mg)**: - **Role in Plants**: L-arginine is an amino acid and a precursor to nitric oxide in plants, synthesized via the nitric oxide synthase (NOS)-like pathway. Nitric oxide regulates processes like seed germination, root development, flowering, and stress resistance (e.g., drought, salinity). Arginine can also serve as a nitrogen source, as plants can metabolize amino acids to support protein synthesis and growth. - **Potential Effects on Cannabis**: Adding L-arginine may increase endogenous NO production, potentially enhancing root growth, nutrient uptake, and stress tolerance. It could promote vigorous vegetative growth or improve flowering by supporting hormonal signaling. Arginine AKG combines arginine with alpha-ketoglutarate, a compound involved in the Krebs cycle, which might provide an additional carbon skeleton for energy metabolism, indirectly supporting growth. - **Risks**: Excessive arginine could disrupt nitrogen balance, as cannabis requires precise ratios of nitrogen, phosphorus, and potassium (NPK). Overloading nitrogen might lead to lush, leafy growth at the expense of bud development or cause toxicity (e.g., dark green leaves, stunted growth). The 400 mg + 400 mg dose is small relative to typical fertilizer applications, but its impact depends on how frequently it’s applied and the plant’s nutrient status. - **L-Citrulline HCl (200 mg) and L-Citrulline Malate (200 mg)**: - **Role in Plants**: L-citrulline is another amino acid and a precursor to L-arginine in plants, indirectly contributing to NO production. It’s less studied in plants than arginine but may similarly support nitrogen metabolism and stress responses. Citrulline malate, which pairs citrulline with malic acid, could provide malate, a compound in the Krebs cycle, potentially aiding energy metabolism. - **Potential Effects on Cannabis**: Similar to arginine, citrulline may enhance NO signaling, promoting root development, nutrient transport, and stress resilience. The malate component could improve metabolic efficiency, potentially boosting photosynthesis or energy availability during flowering. However, the small dose (400 mg total citrulline) limits its impact unless applied consistently. - **Risks**: As with arginine, excessive citrulline could skew nitrogen levels. The HCl component in citrulline HCl might slightly lower soil pH, which could be beneficial for cannabis (optimal pH 6.0–7.0 for soil, 5.5–6.5 for hydroponics) but requires monitoring to avoid acidification. - **Betaine (60 mg)**: - **Role in Plants**: Betaine (also known as glycine betaine) is an osmoprotectant that helps plants cope with stress, such as drought, salinity, or temperature extremes, by stabilizing proteins and membranes. It’s naturally produced by some plants but can be supplemented to enhance stress tolerance. - **Potential Effects on Cannabis**: Betaine could improve cannabis resilience to environmental stressors, such as high temperatures or water deficiency, which is valuable during flowering when plants are sensitive. It may also support photosynthesis under stress by protecting chloroplasts. The 60 mg dose is minimal, so effects may be subtle unless accumulated over time. - **Risks**: Betaine is generally safe, but excessive application could interfere with osmotic balance or nutrient uptake if not balanced with other nutrients. - **General Notes on Nitric Oxide Pill**: - The pill’s components are water-soluble and could be dissolved for foliar or root application, but their efficacy in cannabis depends on concentration, application method, and plant stage. Cannabis benefits from NO signaling, but the low doses in a single pill (1.26 g total active ingredients) are unlikely to cause dramatic effects unless applied regularly. - **Caution**: These supplements are formulated for human digestion, not plants. Inactive ingredients (e.g., fillers, binders) in the pill could introduce unwanted compounds to the soil or plant, potentially affecting microbial activity or causing residue buildup in buds, which could be harmful if consumed. ### 2. **TMG Powder (1/8 Teaspoon Trimethylglycine)** - **Role in Plants**: Trimethylglycine (TMG), also called glycine betaine, is identical to the betaine in the nitric oxide pill. It acts as an osmoprotectant and methyl donor, supporting stress tolerance and metabolic processes. A 1/8 teaspoon (~0.625 g or 625 mg) is a moderate dose compared to the 60 mg in the pill. - **Potential Effects on Cannabis**: TMG could enhance stress resistance, particularly during flowering, when cannabis is prone to heat or drought stress. It may improve water retention and photosynthetic efficiency, potentially leading to healthier plants and better bud quality. Combined with the pill’s betaine, the total dose (685 mg) is still modest but more significant than betaine alone. - **Risks**: Overapplication of TMG could disrupt osmotic balance or lead to excessive vegetative growth if nitrogen levels are already high. Soil microbes may break down TMG, reducing its efficacy over time. ### 3. **Quick Sorb Amino Acid (Five Drops)** - **Role in Plants**: Without specific details on the composition of “Quick Sorb Amino Acid,” I’ll assume it’s a liquid amino acid supplement, likely containing a mix of free amino acids (e.g., glutamine, glycine, aspartic acid). Amino acids provide organic nitrogen, support protein synthesis, and act as stress mitigators. They can enhance nutrient uptake, stimulate growth hormones, and improve photosynthesis. - **Potential Effects on Cannabis**: Five drops (likely ~0.25–0.5 mL) is a small dose, but amino acids can promote vegetative growth, root development, and bud formation by providing readily available nitrogen and stimulating metabolic pathways. They may also enhance terpene and cannabinoid production by supporting enzymatic activity during flowering. - **Risks**: Overuse could lead to nitrogen excess, causing leafy growth or nutrient burn. The small dose minimizes this risk, but the exact composition and concentration matter. If the product contains stabilizers or preservatives, these could affect soil health or plant uptake. ### 4. **Advanced Nutrients and Connoisseur Bloom (Normal Dosage)** - **Role in Plants**: Advanced Nutrients and Connoisseur Bloom are commercial cannabis fertilizers designed to optimize growth and flowering. Connoisseur Bloom is a two-part (A and B) nutrient formula with tailored NPK ratios (e.g., 2-2-4 or similar for bloom phase), micronutrients, and proprietary additives like amino acids, humic acids, and bloom enhancers. “Normal dosage” typically follows manufacturer guidelines (e.g., 4 mL/L for each part in bloom phase). - **Potential Effects on Cannabis**: These products are formulated to maximize bud yield, density, and potency by providing balanced macronutrients (nitrogen, phosphorus, potassium), micronutrients (calcium, magnesium, iron), and biostimulants. They support robust flowering, resin production, and terpene development, which are critical for high-quality cannabis. - **Risks**: Overdosing can cause nutrient burn (yellowing or crispy leaf tips), while underdosing may limit yield. Combining with other nitrogen-rich supplements (e.g., arginine, citrulline, amino acids) increases the risk of nitrogen overload, which could delay flowering or reduce bud quality. ### 5. **ATAMI Bloombastic (1 mL)** - **Role in Plants**: ATAMI Bloombastic is a bloom booster containing high levels of phosphorus and potassium (PK, typically ~14-15 NPK ratio), bio-stimulants, and sugars. It’s designed to enhance flower size, density, and aroma in the late flowering stage. The recommended dose is 0.5–1 mL/L, so 1 mL is within the standard range for a small reservoir (e.g., 1–2 liters). - **Potential Effects on Cannabis**: Bloombastic promotes larger, denser buds with enhanced terpene and cannabinoid profiles due to its PK boost and bio-stimulants. It’s particularly effective in weeks 4–8 of flowering, improving yield and quality. Combined with Connoisseur Bloom, it provides a comprehensive nutrient profile for flowering. - **Risks**: Overuse can cause nutrient lockout or burn, especially if phosphorus and potassium levels are already high from Connoisseur Bloom. The sugars in Bloombastic may also feed soil microbes, which could be beneficial but might alter soil dynamics if not balanced. ### **Combined Effects on Marijuana Plants** - **Vegetative Growth**: The nitric oxide pill (arginine, citrulline) and quick sorb amino acids provide organic nitrogen, potentially boosting vegetative growth by supporting protein synthesis and NO signaling. This could lead to stronger stems, larger leaves, and better root systems, especially early in the growth cycle. TMG and betaine enhance stress tolerance, which could improve plant vigor under suboptimal conditions (e.g., high heat, low humidity). - **Flowering Phase**: Connoisseur Bloom and ATAMI Bloombastic are tailored for flowering, promoting bud development, resin production, and aroma. The NO precursors (arginine, citrulline) may enhance nutrient transport to flowers via improved vascular function, potentially increasing bud size or potency. However, excess nitrogen from amino acids could delay flowering or reduce bud quality by favoring leaf growth. - **Stress Resistance**: Betaine and TMG are likely to improve resilience to environmental stressors, which is critical during flowering when cannabis is sensitive to fluctuations. NO from arginine and citrulline may also mitigate oxidative stress, protecting plants from damage due to light intensity or nutrient imbalances. - **Synergies and Risks**: - **Synergies**: The combination of NO precursors, amino acids, and bloom nutrients could create a synergistic effect, enhancing nutrient uptake, energy metabolism, and flower development. For example, NO may improve the efficiency of phosphorus and potassium delivery from Bloombastic and Connoisseur Bloom. - **Risks**: The primary concern is nitrogen overload from the cumulative effect of arginine, citrulline, amino acids, and Connoisseur Bloom. Cannabis in the flowering stage requires lower nitrogen and higher phosphorus/potassium, so this mix could disrupt NPK balance, leading to leafy buds or nutrient burn. The pill’s inactive ingredients and the quick sorb’s unknown additives pose risks of soil contamination or residue in buds, which could affect safety for consumption. Additionally, the small doses of the pill and TMG may have minimal impact unless applied frequently, but frequent application increases the risk of over-fertilization. ### **Application Considerations** - **Dosage and Frequency**: The doses listed (e.g., one pill, 1/8 tsp TMG, five drops amino acid, 1 mL Bloombastic) are small, suggesting they’re intended for a single application or small reservoir. For significant effects, you’d need to scale up and apply consistently (e.g., weekly), but this requires careful monitoring to avoid nutrient buildup. Follow Advanced Nutrients’ guidelines for Connoisseur Bloom (e.g., 4 mL/L) and adjust other supplements to avoid excess. - **Application Method**: Dissolve the nitric oxide pill and TMG in water for root drench or foliar spray, ensuring even distribution. Quick sorb amino acids are likely best applied as a foliar spray for rapid uptake. Connoisseur Bloom and Bloombastic should be mixed into the nutrient solution per manufacturer instructions, typically for hydroponics or soil. - **Plant Stage**: Use high-nitrogen supplements (arginine, citrulline, amino acids) sparingly in the flowering stage to avoid delaying bud development. Focus on Connoisseur Bloom and Bloombastic during flowering for optimal results. Betaine and TMG can be applied throughout the cycle to enhance stress tolerance. - **Monitoring**: Regularly check leaf color, growth patterns, and soil/runoff pH and EC (electrical conductivity) to detect nutrient imbalances. Signs of nitrogen excess include dark green leaves, clawing, or slow flowering. Phosphorus/potassium excess may cause leaf burn or lockout of other nutrients. ### **Scientific Context and Gaps** - **Nitric Oxide in Plants**: Research shows NO regulates stomatal closure, root growth, and stress responses in plants, but most studies focus on model plants (e.g., Arabidopsis) rather than cannabis. Arginine and citrulline’s role as NO precursors is plausible but not well-documented for cannabis specifically.[](https://pmc.ncbi.nlm.nih.gov/articles/PMC9710401/) - **Betaine/TMG**: Studies confirm betaine’s role in stress tolerance, but optimal doses for cannabis are unclear. The combined 685 mg dose is within a reasonable range but requires testing.[](https://www.hollandandbarrett.com/the-health-hub/vitamins-and-supplements/amino-acids/arginine/hidden-health-benefits-l-arginine/) - **Cannabis Nutrients**: Advanced Nutrients and Bloombastic are well-established for cannabis, with proven effects on yield and quality when used correctly. However, combining with unconventional supplements like nitric oxide pills lacks empirical data. - **Lack of Cannabis-Specific Research**: No studies directly address this combination in cannabis, so effects are inferred from general plant science and cannabis nutrient requirements. Experimentation is needed to validate efficacy and safety. ### **Recommendations** 1. **Start with a Control**: Test this combination on a small number of plants, comparing to a control group using only Connoisseur Bloom and Bloombastic to assess benefits or drawbacks. 2. **Adjust Nitrogen Levels**: Reduce the frequency or dose of the nitric oxide pill and quick sorb amino acids during flowering to avoid nitrogen excess. Focus on these during vegetative growth if needed. 3. **Monitor Nutrient Balance**: Use a nutrient calculator to ensure NPK ratios align with cannabis needs (e.g., 1:2:3 during flowering). Check EC (1.2–2.0 mS/cm in bloom) and pH regularly. 4. **Safety for Consumption**: Avoid applying the nitric oxide pill or quick sorb close to harvest (within 2–3 weeks) to minimize residue in buds. Flush plants with plain water for 1–2 weeks before harvest to reduce chemical buildup. 5. **Alternative NO Sources**: If NO production is the goal, consider safer plant-specific NO donors (e.g., sodium nitroprusside in research settings) or focus on optimizing nutrient delivery with proven fertilizers.[](https://pmc.ncbi.nlm.nih.gov/articles/PMC9710401/) ### **Conclusion** The combination of a nitric oxide pill, TMG, quick sorb amino acids, Advanced Nutrients Connoisseur Bloom, and ATAMI Bloombastic could theoretically enhance cannabis growth, stress tolerance, and bud quality by boosting NO signaling, nutrient uptake, and PK availability. However, the unconventional use of human supplements introduces risks of nitrogen overload, nutrient imbalance, and potential residues. Connoisseur Bloom and Bloombastic are likely sufficient for optimal flowering, with the other components offering marginal benefits at best and risks of over-fertilization at worst. Proceed cautiously, test on a small scale, and prioritize proven cannabis nutrients for consistent results. 7/25 9am 💦💦💦. water today with nutrients and amino acids and TMG powder that seems to have an immediate and striking affect although I am getting more nitrogen than I would like at this phase so I will be toning everything back but really happy with this experiment.👍👍👍 7/26 3am I really have to trim them down to four colors each. I didn’t expect to have four plants. I expected to have two I didn’t know they were so strong and they would all survive and the strange thing is the one that has the least amount of dirt in the pot the one that I stressed the most is growing the best and I think it’s because of the amino treatments, but I won’t be doing any more of that just normal nutrients from here on now🚨👍👍👍👍. 7/27 513am.... DIE HARD CHRISTMAS GROW!!!!!! IS THAT A GERANIMALS SUIT FROM HONG KONG? I HAVE 4...(OVERTLY NASELY VILLIAN VOICE...) GROW TENT LOOKS LIKE THE VENT SYSTEM could work.. have to spend 4 months grocery money on Bruce Willis props...I dont know. 1245 pm. Determining the appropriate amount of betaine to use in a 2-liter nutrient solution for autoflowering cannabis plants, alongside Advanced Nutrients pH Perfect Bloom and Atami Bloombastic (referred to as “Tammy Bloom tastic”), requires careful consideration due to the lack of cannabis-specific studies on betaine dosing and the sensitivity of autoflowers to nutrient concentrations. Below, I’ll provide a reasoned approach based on general plant studies, the characteristics of autoflowers, and the provided nutrient context, ensuring compatibility with Advanced Nutrients Bloom and Atami Bloombastic. Key Considerations 1 Autoflower Sensitivity: Autoflowering cannabis plants require lower nutrient concentrations (typically 1/4 to 1/2 the strength of photoperiod plants) due to their compact size, smaller root systems, and Cannabis ruderalis genetics, which evolved in low-nutrient environments. Overdosing can cause nutrient burn, stunted growth, or reduced bud quality. 2 Betaine’s Role: Betaine (trimethylglycine or betaine anhydrous) acts as an osmoprotectant and stress mitigator, enhancing plant resilience to abiotic stresses (e.g., salinity, drought, temperature extremes) and potentially improving growth and photosynthetic efficiency. Studies on other plants (e.g., peppers, Suaeda salsa) suggest effective concentrations of 1–20 mM for foliar or root application, but cannabis-specific data is absent. 3 Advanced Nutrients pH Perfect Bloom: This is a base nutrient solution designed for the flowering phase, providing macronutrients (N-P-K) and micronutrients with pH-stabilizing technology. The recommended dosage for autoflowers is typically 1–2 mL/L (1/4 to 1/2 strength of photoperiod recommendations, which are 4 mL/L). For a 2-liter solution, 2–4 mL total is standard for autoflowers to avoid overfeeding. 4 Atami Bloombastic (1 mL): Bloombastic is a bloom enhancer with high phosphorus and potassium (0-14-15 N-P-K), designed to increase bud weight, flavor, and density. The recommended dosage is 0.5–1 mL/L, with 2–4 mL/gallon (3.78 L) in the final 4 weeks of flowering. For a 2-liter solution, 1 mL (0.5 mL/L) is appropriate for autoflowers, aligning with the cautious dosing recommended for their sensitivity. 5 Betaine Dosing for Plants: Studies suggest exogenous betaine concentrations of 1–5 mM for soil/hydroponic applications and 10–20 mM for foliar sprays are effective for stress mitigation and growth enhancement in plants like peppers and alfalfa. Higher concentrations (e.g., 50 mM) may be less effective or cause osmotic stress. For autoflowers, starting at the lower end is critical to avoid nutrient imbalances. Calculating Betaine Dosage • Molecular Weight of Betaine: Betaine anhydrous (trimethylglycine) has a molecular weight of 117.15 g/mol. • Molarity to Mass Conversion: To achieve a desired molar concentration (e.g., 1–5 mM) in a 2-liter solution: ◦ 1 mM = 1 mmol/L = 0.001 mol/L. ◦ Mass of betaine per liter = molarity × molecular weight. ◦ For 1 mM: 0.001 mol/L × 117.15 g/mol = 0.11715 g/L = 117.15 mg/L. ◦ For 2 L at 1 mM: 117.15 mg/L × 2 L = 234.3 mg. ◦ For 5 mM: 0.005 mol/L × 117.15 g/mol = 0.58575 g/L = 585.75 mg/L. ◦ For 2 L at 5 mM: 585.75 mg/L × 2 L = 1171.5 mg (1.17 g). • Recommended Range: Based on plant studies, a concentration of 1–5 mM (🚨🚨🚨234.3–1171.5 mg per 2 L🚨🚨) is likely effective for root application in hydroponics or soil, balancing efficacy and safety for autoflowers. Recommended Betaine Dosage For a 2-liter nutrient solution with Advanced Nutrients pH Perfect Bloom and 1 mL Atami Bloombastic, I recommend starting with a low betaine concentration to ensure compatibility and avoid overloading autoflowers: • Betaine Dosage: 250–500 mg per 2 L (approximately 1–2 mM). ◦ Rationale: This corresponds to 125–250 mg/L, within the 1–5 mM range effective in studies for stress mitigation and growth enhancement (e.g., peppers showed improved root activity and biomass at 5 mM). Autoflowers require lower nutrient strengths, so starting at 1–2 mM minimizes the risk of nutrient burn or osmotic stress, especially when combined with nutrient-rich products like Bloom and Bloombastic. Advanced Nutrients Bloom (at 1–2 mL/L, or 2–4 mL per 2 L) and Bloombastic (0.5 mL/L, or 1 mL per 2 L) already provide significant macronutrients (N-P-K) and micronutrients, so betaine should complement, not overwhelm, the nutrient profile. ◦ Application: Dissolve 250–500 mg of betaine anhydrous powder in the 2-liter solution after adding Advanced Nutrients Bloom (2–4 mL) and Atami Bloombastic (1 mL). Mix thoroughly to ensure complete dissolution, as betaine is highly water-soluble. Application Guidelines • Timing: Apply during the vegetative phase (weeks 2–4) or early flowering (weeks 4–6) to support stress tolerance and growth. Avoid late flowering (weeks 8–10), as autoflowers benefit from a nutrient flush to enhance bud flavor. • Method: Add betaine to the nutrient solution for soil or hydroponic systems. For foliar application, consider a higher concentration (10–20 mM, or 1.17–2.34 g per 2 L), but this is less practical for indoor grows due to potential leaf residue and should be tested separately. • Monitoring: Watch for signs of nutrient burn (e.g., brown leaf tips, wilting) or overfeeding, as autoflowers are sensitive. Start with 250 mg (1 mM) and increase to 500 mg (2 mM) after 1–2 applications if plants respond well (e.g., vibrant green leaves, robust growth). • pH and EC: Advanced Nutrients pH Perfect Bloom stabilizes pH (typically 5.5–6.5 for hydro, 6.0–6.5 for soil), so additional pH adjustment is usually unnecessary. However, check electrical conductivity (EC) to ensure the total nutrient strength remains in the autoflower-friendly range (0.8–1.4 mS/cm for vegetative, 1.2–1.8 mS/cm for flowering). Betaine may slightly increase EC, so measure after mixing. Precautions • Nutrient Balance: Advanced Nutrients Bloom and Bloombastic provide high phosphorus and potassium, critical for flowering. Excessive betaine could disrupt nutrient uptake (e.g., by altering osmotic balance), so stay within 1–2 mM. • Betaine Source: Use betaine anhydrous (not betaine HCl, which is for digestive support and unsuitable for plants). Ensure the product is pure (99%+), as some supplements contain additives like silicon dioxide. • Flushing: In the final 1–2 weeks of the autoflower cycle, use plain water or a flushing agent (e.g., Advanced Nutrients Flawless Finish) to remove excess nutrients, including betaine, to avoid affecting bud taste. • Testing: Since betaine’s effects on cannabis are not well-documented, start with 250 mg per 2 L and observe plant response for 1–2 weeks before increasing to 500 mg. Example Nutrient Solution Recipe for 2 L • Water: 2 liters (preferably reverse osmosis or dechlorinated tap water). • Advanced Nutrients pH Perfect Bloom: 2–4 mL (1–2 mL/L, adjusted based on plant stage and health; start with 2 mL for early flowering). • Atami Bloombastic: 1 mL (0.5 mL/L, suitable for autoflower flowering phase). • Betaine Anhydrous: 250 mg (1 mM) for initial application, increase to 500 mg (2 mM) if plants tolerate well. • Mixing: Add Bloom first, mix thoroughly, then add Bloombastic, mix again, and finally dissolve betaine. Check EC (target 1.2–1.8 mS/cm for flowering autoflowers) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). Summary For a 2-liter nutrient solution with Advanced Nutrients pH Perfect Bloom (2–4 mL) and Atami Bloombastic (1 mL), add 250–500 mg of betaine anhydrous (1–2 mM) to support stress tolerance and growth in autoflowering cannabis. Start with 250 mg during the vegetative or early flowering phase, monitor for nutrient burn, and increase to 500 mg if plants respond positively. Mix thoroughly, check EC, and avoid late-flowering applications to ensure optimal bud quality. Use betaine anhydrous, not betaine HCl, and source from a reputable supplier. If you provide details about your grow setup (e.g., hydroponic, soil, indoor/outdoor, specific autoflower strain), I can refine the dosage or suggest additional considerations. Let me know! 7/27 230pm. Adding proline to a 2-liter nutrient solution containing Advanced Nutrients pH Perfect Bloom (2–4 mL), Atami Bloombastic (1 mL), and betaine anhydrous (250–500 mg, 1–2 mM) for autoflowering cannabis plants could potentially enhance stress tolerance and growth, as proline is another well-documented osmoprotectant and stress-mitigating compound in plants. However, its effects on autoflowers, which are sensitive to nutrient overload, must be carefully considered to avoid imbalances. Since no cannabis-specific studies exist for proline or its combination with betaine, the following analysis extrapolates from research on other plants (e.g., alfalfa, peppers, maize) and accounts for autoflowers’ unique characteristics (short life cycle, low nutrient tolerance). Background on Proline • What is Proline?: Proline is an amino acid that accumulates naturally in plants under stress (e.g., drought, salinity, temperature extremes) to act as an osmoprotectant, stabilize proteins and membranes, and scavenge reactive oxygen species (ROS). It complements betaine’s role as an osmolyte and stress mitigator. • Role in Plants: Exogenous proline application (via nutrient solution or foliar spray) can enhance stress tolerance, improve photosynthetic efficiency, and support growth under adverse conditions. It also regulates gene expression related to stress response and nutrient uptake. • Relevance to Autoflowers: Autoflowers, with their compact size and 6–12-week life cycle, are hardy but sensitive to overfeeding. Proline could enhance resilience in stressful environments (e.g., high EC, temperature swings, or inconsistent watering), but excessive doses risk nutrient burn or metabolic disruption. Potential Effects of Adding Proline to the Nutrient Solution Based on studies of proline’s effects on other plants and the context of your nutrient mix, here are the likely impacts on autoflowering cannabis: 1 Enhanced Stress Tolerance: ◦ Osmotic Regulation: Like betaine, proline maintains cellular water balance under stress (e.g., drought, salinity). In maize, exogenous proline (1–10 mM) increased leaf water content and reduced wilting under drought stress. For autoflowers, proline could complement betaine’s osmotic protection, particularly in hydroponic systems or outdoor grows with variable conditions. ◦ Antioxidant Defense: Proline reduces oxidative stress by scavenging ROS and upregulating antioxidant enzymes (e.g., superoxide dismutase [SOD], peroxidase [POD]). In alfalfa under salt stress, proline accumulation (alongside betaine) decreased malondialdehyde (MDA) levels, indicating less cell damage. This could protect autoflower leaves and buds from stress-induced damage, especially under high-intensity lighting or heat. 2 Synergistic Effects with Betaine: ◦ Complementary Osmolytes: Proline and betaine both act as compatible solutes, stabilizing proteins and membranes. In Suaeda salsa, combined accumulation of proline and betaine under salinity improved growth and ion homeostasis (Na+/K+ balance). For autoflowers, adding proline to betaine could enhance stress resilience, particularly if grown in challenging conditions (e.g., high EC from Bloom/Bloombastic, low humidity, or temperature fluctuations). ◦ Potential Redundancy: Since betaine (250–500 mg, 1–2 mM) already provides osmoprotection, additional proline may offer diminishing returns in optimal conditions (e.g., controlled indoor grows with 18–20 hours light, 70–80°F, 40–60% humidity). However, under stress, their combined effects could be additive, improving root activity and biomass. 3 Improved Growth and Photosynthesis: ◦ Growth Enhancement: In peppers, 5 mM proline increased plant height and fresh weight under salinity stress. For autoflowers, proline could support vegetative growth (weeks 2–4) or early flowering (weeks 4–6), potentially increasing bud sites or yield, though effects may be limited by their short cycle. ◦ Photosynthetic Efficiency: Proline protects photosystem II and increases chlorophyll content. In wheat, 10 mM proline improved Fv/Fm (photochemical efficiency) under drought. For autoflowers under high-intensity lights (e.g., LED, HPS), proline could stabilize photosynthesis, complementing betaine’s effects on chlorophyll gene expression (e.g., Capsb genes in peppers). 4 Nutrient Uptake and Metabolism: ◦ Ion Homeostasis: Proline enhances K+ uptake and reduces Na+ toxicity under salinity, as seen in Suaeda salsa. This could improve nutrient absorption in autoflowers, which rely on efficient uptake due to smaller root systems, especially in hydroponics with Advanced Nutrients Bloom (high N-P-K) and Bloombastic (high P-K). ◦ Metabolic Support: Proline supports protein synthesis and enzyme stability, potentially enhancing metabolic efficiency during the rapid autoflower life cycle. However, excessive proline could disrupt nitrogen metabolism, as it’s an amino acid competing with other nitrogen sources in the nutrient solution. 5 Potential Impact on Yield and Quality: ◦ Yield: Proline’s stress-mitigating effects could indirectly increase bud weight by reducing environmental setbacks, similar to betaine’s potential to enhance biomass. Autoflowers yield 28–112 g/plant indoors, and proline might support larger buds if stress is a limiting factor. ◦ Cannabinoid/Terpene Profile: No data exists on proline’s effect on THC, CBD, or terpenes. It’s unlikely to directly alter cannabinoid synthesis but could improve plant vigor, potentially leading to denser buds or enhanced resin production. Overapplication risks altering flavor if not flushed properly. Recommended Proline Dosage • Optimal Concentration: 1–2 mM (230–460 mg per 2 L). ◦ Calculation: Proline molecular weight: 115.13 g/mol. 1 mM = 0.001 mol/L × 115.13 g/mol = 0.11513 g/L = 115.13 mg/L. For 2 L at 1 mM: 115.13 mg/L × 2 L = 230.26 mg (rounded to 230 mg). For 2 L at 2 mM: 115.13 mg/L × 2 × 2 L = 460.52 mg (rounded to 460 mg). ◦ Rationale: Studies (e.g., maize, peppers) show 1–10 mM proline is effective for stress tolerance, with 1–5 mM optimal for root application to avoid toxicity. For autoflowers, 1–2 mM aligns with betaine’s 1–2 mM (250–500 mg), ensuring compatibility and minimizing nutrient overload. Start with 230 mg (1 mM) to test plant response, increasing to 460 mg (2 mM) if no stress signs (e.g., leaf burn, stunted growth) appear after 1–2 applications. • Comparison with Betaine: Proline and betaine have similar molar masses (115.13 vs. 117.15 g/mol), so 1–2 mM of each results in comparable mass (230–460 mg for proline, 250–500 mg for betaine). Their combined effect should stay within autoflower EC limits (1.2–1.8 mS/cm for flowering). Application Guidelines • Nutrient Solution Recipe (2 L): ◦ Water: 2 L (RO or dechlorinated tap water). ◦ Advanced Nutrients pH Perfect Bloom: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). ◦ Atami Bloombastic: 1 mL (0.5 mL/L, suitable for flowering autoflowers). ◦ Betaine Anhydrous: 250–500 mg (1–2 mM, start with 250 mg). ◦ Proline: 230–460 mg (1–2 mM, start with 230 mg). ◦ Mixing: Add Bloom first, then Bloombastic, betaine, and proline, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). • Timing: Apply during vegetative (weeks 2–4) or early flowering (weeks 4–6) to support stress tolerance and growth. Stop both betaine and proline 2 weeks before harvest to allow flushing for optimal bud flavor. • Frequency: Use with each nutrient feeding (e.g., every 2–3 days in hydro, weekly in soil). Alternate or reduce frequency if EC approaches 1.8 mS/cm. • Source: Use L-proline (plant-grade, 99%+ purity) from a reputable supplier (e.g., agricultural or lab-grade sources). Avoid impure or non-plant-specific products. Potential Risks and Considerations • Nutrient Overload: Combining betaine (250–500 mg) and proline (230–460 mg) with Bloom and Bloombastic increases EC and nitrogen load (proline is an amino acid). Monitor for nutrient burn (brown leaf tips, clawing). If symptoms appear, reduce both to 0.5 mM (125 mg betaine, 115 mg proline per 2 L) or skip a feeding. • EC Impact: Betaine and proline each add ~0.1–0.2 mS/cm at 1–2 mM. With Bloom (1–2 mL/L) and Bloombastic (0.5 mL/L) at ~1.0–1.5 mS/cm, the total EC should stay below 1.8 mS/cm. Measure after mixing. • Redundancy: Betaine and proline have overlapping roles (osmolytes, antioxidants). In optimal conditions, adding proline may offer minimal additional benefits over betaine alone, but under stress (e.g., high heat, low humidity), their synergy could enhance resilience. • Cost vs. Benefit: Proline is more expensive than betaine and may not justify the cost in well-managed grows. Test its impact on a small scale first. • Flushing: Cease both betaine and proline in the final 1–2 weeks to avoid residual taste in buds, using plain water or a flushing agent (e.g., Advanced Nutrients Flawless Finish). • Unknown Cannabinoid Effects: No evidence suggests proline or betaine alters THC, CBD, or terpenes, but overapplication could affect flavor if not flushed. Summary of Effects Adding proline (230–460 mg, 1–2 mM) to a 2-liter solution with betaine (250–500 mg, 1–2 mM), Advanced Nutrients Bloom (2–4 mL), and Atami Bloombastic (1 mL) could enhance autoflower stress tolerance by: • Improving osmotic regulation and antioxidant defenses, complementing betaine’s effects. • Supporting photosynthesis and growth under stress (e.g., salinity, heat, low light), potentially increasing biomass or bud yield. • Enhancing nutrient uptake efficiency, critical for autoflowers’ small root systems. Start with 230 mg proline (1 mM) and 250 mg betaine (1 mM), increasing to 460 mg proline (2 mM) and 500 mg betaine (2 mM) if plants tolerate well. Apply during vegetative or early flowering, monitor EC (1.2–1.8 mS/cm), and stop 2 weeks before harvest. Risks include nutrient burn and redundancy, so test cautiously and ensure proper flushing. If you provide your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine the dosing or application method. Let me know if you need sourcing tips for proline or further guidance!”” 7/27 3 pm. SILICA. Adding **0.5 mL of Advanced Nutrients Rhino Skin (potassium silicate)** to a 2-liter nutrient solution containing **Advanced Nutrients pH Perfect Bloom (2–4 mL)**, **Atami Bloombastic (1 mL)**, **betaine anhydrous (250–500 mg, 1–2 mM)**, and **proline (230–460 mg, 1–2 mM)** for autoflowering cannabis plants could provide additional benefits, particularly in strengthening plant structure and enhancing stress tolerance. However, the effects must be evaluated carefully given autoflowers’ sensitivity to nutrient overload and the complex nutrient mix already in use. Below, I analyze the potential effects of adding 0.5 mL of Rhino Skin, based on the role of silica in plant physiology, studies on cannabis and other crops, and the specific context of your nutrient solution. ### **Background on Silica (Advanced Nutrients Rhino Skin)** - **What is Rhino Skin?**: Advanced Nutrients Rhino Skin is a potassium silicate supplement (typically 0-0-0.4 N-P-K, with ~4–7% silica as SiO₂). Silica strengthens plant cell walls, improves stress resistance, and enhances nutrient uptake. The recommended dosage is 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L) due to their sensitivity. - **Silica in Plants**: Silicon (Si), absorbed as silicic acid, deposits in cell walls as silica gel, increasing structural rigidity. It also mitigates abiotic (e.g., drought, heat) and biotic (e.g., pests, fungi) stresses and can improve photosynthesis and yield. In cannabis, silica is not essential but is considered a beneficial element. - **Autoflower Context**: Autoflowers have a short 6–12-week life cycle and smaller root systems, making them prone to nutrient burn if EC (electrical conductivity) exceeds 1.2–1.8 mS/cm during flowering. Rhino Skin’s low dosage (0.5 mL per 2 L, or 0.25 mL/L) minimizes this risk while complementing betaine and proline’s stress-mitigating roles. ### **Potential Effects of Adding 0.5 mL Rhino Skin** Based on silica’s effects in cannabis and other plants (e.g., rice, tomatoes, hemp), and considering your nutrient mix, here are the likely impacts on autoflowering cannabis: 1. **Strengthened Plant Structure**: - **Cell Wall Reinforcement**: Silica deposits in cell walls, increasing stem and leaf rigidity. In hemp (*Cannabis sativa*), silica supplementation (e.g., 1–2 mM Si) thickened stems and improved mechanical strength. For autoflowers, which are compact (1–3 feet tall), 0.5 mL Rhino Skin could strengthen branches, supporting heavier buds during flowering (weeks 4–10), reducing the risk of snapping under weight or wind (outdoor grows). - **Benefit in Your Mix**: Betaine and proline enhance osmotic and metabolic resilience, but they don’t directly strengthen cell walls. Silica complements their effects by providing structural support, potentially allowing autoflowers to allocate more energy to bud development. 2. **Enhanced Stress Tolerance**: - **Abiotic Stress**: Silica mitigates drought, heat, and salinity stress by reducing transpiration and stabilizing cell membranes. In tomatoes, 1 mM silica improved water retention under drought. For autoflowers, 0.5 mL Rhino Skin (~0.1–0.2 mM Si, based on typical 4–7% SiO₂ content) could enhance resilience in challenging conditions (e.g., low humidity, high temperatures, or high EC from Bloom/Bloombastic). - **Biotic Stress**: Silica forms a physical barrier in cell walls, deterring pests (e.g., spider mites) and pathogens (e.g., powdery mildew). In cannabis, silica reduced fungal infections in controlled studies. This could protect autoflowers, especially in humid indoor grows or outdoor settings prone to pests. - **Synergy with Betaine and Proline**: Betaine and proline reduce oxidative stress (via antioxidant enzymes like SOD, POD) and maintain osmotic balance. Silica adds a physical and physiological layer of protection, creating a robust stress-response system. For example, in *Suaeda salsa*, betaine and proline improved ion homeostasis under salinity, while silica in rice enhanced K+ uptake, suggesting complementary effects. 3. **Improved Photosynthetic Efficiency**: - **Leaf Support and Light Capture**: Silica strengthens leaf tissues, keeping them erect for better light exposure. In rice, 1.5 mM silica increased chlorophyll content and photosynthetic rates. For autoflowers under high-intensity lights (18–24 hours), 0.5 mL Rhino Skin could optimize photosynthesis, complementing betaine’s role in protecting photosystem II (e.g., upregulation of Capsb genes in peppers). - **Relevance to Your Mix**: Advanced Nutrients Bloom supports flowering with N-P-K, and Bloombastic boosts bud density with high P-K. Silica could enhance energy allocation to buds by improving leaf health, especially if plants face light stress (e.g., inconsistent outdoor light). 4. **Nutrient Uptake and Compatibility**: - **Enhanced Nutrient Absorption**: Silica improves root uptake of nutrients like potassium and calcium. In maize, 1 mM silica increased K+ and Ca²⁺ levels. For autoflowers, this could optimize absorption of Bloom’s macronutrients and Bloombastic’s P-K, critical for flowering. - **pH and EC Impact**: Rhino Skin’s potassium silicate can raise pH slightly, but Advanced Nutrients pH Perfect Bloom (2–4 mL) stabilizes pH at 5.5–6.5 (hydro) or 6.0–6.5 (soil). At 0.5 mL per 2 L (0.25 mL/L), Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), keeping the total EC (Bloom + Bloombastic + betaine + proline + silica) within 1.2–1.8 mS/cm for flowering autoflowers. - **Compatibility with Betaine and Proline**: Betaine (1–2 mM) and proline (1–2 mM) are neutral organic compounds that don’t chemically interact with silica (an inorganic compound). Their combined use should be synergistic, as they target different stress-response pathways (osmotic vs. structural). 5. **Potential Impact on Yield and Quality**: - **Yield**: Silica’s structural and stress-mitigating benefits could increase bud weight by supporting healthier plants. In hemp, 1 mM silica increased biomass by 10–15%. For autoflowers (28–112 g/plant indoors), 0.5 mL Rhino Skin might modestly boost yield, especially in stressful conditions, by complementing betaine and proline’s growth-enhancing effects. - **Cannabinoid/Terpene Profile**: No direct evidence links silica to THC, CBD, or terpene changes in cannabis, but stronger plants may produce denser buds or more resin. Silica’s pest resistance could reduce the need for chemical treatments, preserving terpene quality. Proper flushing remains critical to avoid flavor impacts. ### **Recommended Dosage and Application** - **Dosage**: **0.5 mL Rhino Skin per 2 L (0.25 mL/L)** is optimal. - **Rationale**: Advanced Nutrients recommends 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L). At 0.5 mL per 2 L, Rhino Skin provides ~0.1–0.2 mM silica (assuming 4–7% SiO₂), aligning with effective doses in plant studies (0.5–2 mM) while minimizing EC and pH impacts. - **Confirmation**: Your specified 0.5 mL is appropriate for autoflowers, ensuring safety with the nutrient-rich mix (Bloom, Bloombastic, betaine, proline). - **Nutrient Solution Recipe (2 L)**: - **Water**: 2 L (RO or dechlorinated tap water). - **Advanced Nutrients pH Perfect Bloom**: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). - **Atami Bloombastic**: 1 mL (0.5 mL/L, suitable for flowering). - **Betaine Anhydrous**: 250–500 mg (1–2 mM, start with 250 mg). - **Proline**: 230–460 mg (1–2 mM, start with 230 mg). - **Advanced Nutrients Rhino Skin**: 0.5 mL (0.25 mL/L). - **Mixing**: Add Bloom first, then Bloombastic, betaine, proline, and finally Rhino Skin, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Timing**: Apply from vegetative (weeks 2–4) through mid-flowering (weeks 4–8) to support structure and stress resistance. Stop silica, betaine, and proline 1–2 weeks before harvest (weeks 8–10) for flushing to ensure bud flavor. - **Frequency**: Use with each nutrient feeding (every 2–3 days in hydro, weekly in soil). If EC nears 1.8 mS/cm, reduce Bloom to 2 mL or alternate silica applications. ### **Potential Risks and Considerations** - **Nutrient Overload**: The mix (Bloom + Bloombastic + betaine + proline + silica) is nutrient-dense. At 0.5 mL, Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), but the total EC (1.2–1.8 mS/cm) must be monitored to avoid burn (brown leaf tips, clawing). If symptoms appear, reduce Bloom to 2 mL or skip silica for one feeding. - **pH Stability**: Rhino Skin’s potassium silicate may slightly raise pH, but pH Perfect Bloom should counteract this. Verify pH after mixing (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Redundancy**: Betaine, proline, and silica all enhance stress tolerance (osmotic, metabolic, structural). In optimal conditions (e.g., indoor, 70–80°F, 40–60% humidity), silica’s benefits may be marginal, but under stress (e.g., heat, pests), it adds a complementary layer of protection. - **Flushing**: Stop all additives (silica, betaine, proline) 1–2 weeks before harvest, using plain water or Advanced Nutrients Flawless Finish to avoid residual taste in buds. - **Cost vs. Benefit**: Rhino Skin is relatively affordable, but its benefits may be less pronounced in well-managed grows. Test on a few plants to assess impact. - **Cannabinoid Effects**: No evidence suggests silica alters THC, CBD, or terpenes, but improved plant health could enhance resin production. Overuse risks silica deposits affecting flavor if not flushed. ### **Summary of Effects** Adding **0.5 mL Advanced Nutrients Rhino Skin** to a 2-liter solution with **Bloom (2–4 mL)**, **Bloombastic (1 mL)**, **betaine (250–500 mg)**, and **proline (230–460 mg)** could: - Strengthen stems and leaves, supporting heavier buds. - Enhance resistance to abiotic (drought, heat) and biotic (pests, fungi) stresses. - Improve photosynthetic efficiency and nutrient uptake, complementing betaine and proline’s osmotic and metabolic benefits. - Potentially increase yield (28–112 g/plant) by reducing stress setbacks. The 0.5 mL dose (0.25 mL/L) is safe for autoflowers, keeping EC within 1.2–1.8 mS/cm. Apply during vegetative to mid-flowering, stop 1–2 weeks before harvest, and monitor for nutrient burn. The synergy of silica (structural), betaine (osmotic), and proline (metabolic) could optimize autoflower health, especially under stress. If you share your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine dosing or application further. Let me know if you need sourcing tips or additional guidance!""

CalGonJim 7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!""" 7/31 245am Adding a nitric oxide (NO) capsule containing L-arginine (200 mg), arginine alpha-ketoglutarate (AKG) 2:1 extract (200 mg), L-citrulline HCl (200 mg), L-citrulline malate extract (100 mg), and beta-alanine (40 mg) to the existing mix of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, L-glycine, and methylene blue (MB) for cannabis plants introduces compounds that primarily influence nitrogen metabolism, stress response, and potentially secondary metabolite production. These components are known for their roles in boosting nitric oxide production in animals, but their effects on plants, including cannabis, are less studied. Below, I’ll outline the potential effects of each component in the NO capsule on cannabis plants, their interactions with the existing mix, and the likely outcomes, drawing from plant science principles and inferred mechanisms due to limited cannabis-specific data. ### Effects of Nitric Oxide Capsule Components on Cannabis Plants 1. **L-Arginine (200 mg)**: - **Role in Plants**: L-Arginine is a precursor to nitric oxide (NO) in plants via the nitric oxide synthase-like pathway. NO regulates growth, stress response, and secondary metabolism. In cannabis, arginine (50–500 mg/L) may enhance root development, flowering, and stress tolerance by modulating NO signaling. - **Stress Response**: NO from arginine mitigates oxidative stress from drought, salinity, or heavy metals, potentially protecting cannabis in harsh environments. - **Secondary Metabolites**: NO influences terpene and alkaloid synthesis, possibly increasing cannabinoid (e.g., THC, CBD) or terpene production in cannabis buds, though direct evidence is limited. 2. **Arginine AKG 2:1 Extract (200 mg)**: - **Role in Plants**: Arginine alpha-ketoglutarate combines arginine with alpha-ketoglutarate, a TCA cycle intermediate. In plants, it may enhance nitrogen assimilation and energy metabolism. Alpha-ketoglutarate could support carbon metabolism, boosting photosynthesis and growth in cannabis. - **Synergy**: Provides additional arginine for NO production and supports metabolic pathways, potentially amplifying arginine’s effects. 3. **L-Citrulline HCl (200 mg) and L-Citrulline Malate Extract (100 mg)**: - **Role in Plants**: L-Citrulline is a precursor to arginine in the urea cycle, indirectly boosting NO production. In plants, citrulline (50–500 mg/L) accumulates under stress (e.g., drought), acting as a nitrogen storage compound and osmoprotectant. In cannabis, it may enhance stress tolerance and nitrogen use efficiency. - **Malate Contribution**: Citrulline malate provides malic acid, which supports the TCA cycle and photosynthesis, potentially increasing cannabis biomass and energy for growth. - **Cannabis-Specific**: Citrulline may improve root and shoot growth in hydroponic systems by enhancing nitrogen metabolism. 4. **Beta-Alanine (40 mg)**: - **Role in Plants**: Beta-alanine is a non-protein amino acid and precursor to pantothenate (vitamin B5) and coenzyme A, which are critical for lipid and secondary metabolite synthesis. In plants, it may enhance stress tolerance by supporting metabolic pathways. Its role in cannabis is less studied but could indirectly support terpene or cannabinoid production via coenzyme A. - **Limited Impact**: At 40 mg, the dose is low, so effects may be minimal compared to other components. **Application Notes**: The NO capsule components (total 740 mg) are typically dissolved for human consumption, but for cannabis, they’d be diluted in water (e.g., 740 mg in 1–10 L, yielding ~74–740 mg/L). A lower concentration (e.g., 100–500 mg/L) is safer for foliar or root application to avoid nitrogen overload. ### Synergistic Effects with EAAs, Proline, TMG, Glutamine, L-Glycine, and Methylene Blue The NO capsule components may enhance the existing mix’s effects on cannabis plants: 1. **Growth and Biomass**: - **Arginine/Citrulline + EAAs/Glutamine/Glycine**: Arginine and citrulline boost NO production and nitrogen metabolism, complementing EAAs, glutamine, and glycine’s roles in protein synthesis and nutrient uptake. This could enhance cannabis vegetative growth, root development, and bud size. - **Alpha-Ketoglutarate + Glutamine**: Alpha-ketoglutarate supports the TCA cycle, amplifying glutamine’s role in nitrogen assimilation, potentially increasing photosynthetic efficiency and biomass. - **Beta-Alanine**: May support lipid metabolism, contributing to cell membrane formation and bud quality, though its low dose limits impact. 2. **Stress Resilience**: - **Arginine/Citrulline + Proline/TMG/MB**: Arginine and citrulline’s NO production enhances stress signaling, complementing proline and TMG’s osmoprotectant roles and MB’s antioxidant effects. Glycine’s glutathione production further bolsters stress tolerance. This mix could significantly improve cannabis resilience to drought, heat, or high light, reducing leaf wilting or nutrient stress. - **Cannabis-Specific**: Ideal for outdoor grows or high-intensity indoor setups where environmental stressors are common. 3. **Secondary Metabolite Production**: - **Arginine/Citrulline + Glutamine/TMG/Glycine**: NO from arginine and citrulline may upregulate pathways for cannabinoid and terpene synthesis, as NO influences phenylpropanoid and alkaloid pathways in plants. Glutamine, glycine, and TMG support precursor synthesis (e.g., GABA, purines, methylation), potentially enhancing THC, CBD, or terpene yields. - **Beta-Alanine**: Supports coenzyme A production, which may aid terpene synthesis, though its low dose may limit impact. - **MB Synergy**: MB’s photosynthetic enhancement could provide energy for NO-driven secondary metabolism, amplifying cannabinoid production, though this is speculative. 4. **Nutrient Efficiency and Pathogen Resistance**: - **Arginine/Citrulline + Glycine/EAAs**: Arginine and citrulline improve nitrogen use efficiency, complementing glycine’s chelating properties and EAAs’ nutrient uptake enhancement, optimizing cannabis growth in hydroponics or nutrient-poor soils. - **MB Synergy**: MB’s antimicrobial properties may synergize with NO’s role in plant defense signaling, protecting cannabis from pathogens like *Botrytis* or *Pythium*. ### Potential Risks and Considerations - **Nitrogen Overload**: The NO capsule adds arginine, citrulline, and beta-alanine, all nitrogen-containing compounds, to an already nitrogen-rich mix (EAAs, glutamine, glycine). This increases the risk of nitrogen toxicity in cannabis, causing dark green leaves, clawing, or reduced flowering. Dilute to 100–500 mg/L total and monitor plant response. - **Absorption Competition**: Arginine, citrulline, EAAs, glutamine, and glycine may compete for root or foliar uptake, reducing efficacy. Stagger applications (e.g., NO capsule components in the morning, others in the evening) or alternate days. - **MB Interaction**: MB’s redox activity may interact with NO from arginine/citrulline, potentially amplifying or disrupting signaling if doses are unbalanced. Keep MB at ~1 mg/L (e.g., two drops of 1% solution per liter). - **Beta-Alanine Limited Impact**: At 40 mg, beta-alanine’s effects may be negligible, as plant studies use higher concentrations (e.g., 100–500 mg/L) for measurable benefits. - **Toxicity Risk**: High doses of arginine or citrulline (1000 mg/L) may disrupt nitrogen balance, while MB (3 mg/L) could cause photodynamic damage. Test low doses first. - **Cultivar Variability**: Sativa-dominant cannabis may benefit more from stress mitigation (arginine, citrulline, proline, TMG, MB), while indicas may respond better to growth promotion (EAAs, glutamine, glycine). ### Practical Recommendations - **Dosing**: - NO Capsule: Dissolve one capsule (740 mg total: 200 mg L-arginine, 200 mg arginine AKG, 200 mg L-citrulline HCl, 100 mg L-citrulline malate, 40 mg beta-alanine) in 2–10 L water (~74–370 mg/L). Start at 100–200 mg/L for foliar or root application. - EAAs: 150–300 mg/L - Glutamine: 250–500 mg/L - Proline: 50–100 mg/L - TMG: 0.5–1 g/L - Glycine: 100–500 mg/L - MB: ~1 mg/L (e.g., two drops of 1% solution per liter) - Apply twice weekly during vegetative growth or early flowering. - **Application**: - **Foliar Spray**: Apply during low-light periods (e.g., early morning) to avoid MB phototoxicity. Combine NO capsule components with amino acids for balanced uptake. - **Root Drench**: Suitable for hydroponics or soil, ensuring low MB concentrations to avoid microbial disruption. - **Staggering**: Apply NO capsule + MB separately from amino acids/TMG to minimize uptake competition (e.g., NO/MB in morning, others in evening). - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener leaves, robust growth). Test on a few plants first, especially for MB and NO components, due to limited cannabis data. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. - **Cultivation Context**: - **Hydroponics**: Use lower doses (~100 mg/L NO capsule, 0.5 mg/L MB) to avoid nutrient lockout or microbial imbalance. - **Soil**: Maintain healthy microbiota, as MB and high nitrogen inputs could disrupt beneficial microbes. - **Cultivars**: Adjust based on strain needs (e.g., stress-prone sativas vs. nutrient-hungry indicas). ### Evidence Gaps - **No Specific Studies**: No research addresses this exact combination (NO capsule + EAAs, proline, TMG, glutamine, glycine, MB) in cannabis. Effects are inferred from individual compound studies in plants (e.g., arginine for NO, MB in algae). - **Cannabinoid/Terpene Impact**: NO and MB may enhance secondary metabolism, but direct effects on THC/CBD or terpenes are unconfirmed. Cannabis-specific trials are needed. - **Beta-Alanine Role**: At 40 mg, beta-alanine’s impact is likely minimal, as plant studies use higher doses. - **Long-Term Effects**: Long-term impacts of MB and NO precursors on cannabis growth or soil health are unknown. Short-term use appears safe at low doses. ### Conclusion Adding a nitric oxide capsule (L-arginine, arginine AKG, L-citrulline, citrulline malate, beta-alanine) to EAAs, proline, TMG, glutamine, glycine, and MB likely enhances cannabis growth, stress tolerance, and nutrient efficiency by boosting NO signaling, nitrogen metabolism, and antioxidant defenses. The mix may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. However, nitrogen overload, absorption competition, and MB’s phototoxicity require careful dosing (e.g., 100–200 mg/L NO capsule, 1 mg/L MB). Test on a small scale, monitor closely, and consult a cannabis cultivation expert for optimization. If you provide the application volume (e.g., liters of water), specific strain, or growth stage, I can refine dosing further. Want me to search for studies on NO precursors in plants or focus on a specific aspect (e.g., NO’s role in cannabinoid synthesis)? 7/29 345am!!🚨. Adding methylene blue (MB) to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), glutamine, and L-glycine for cannabis plants introduces a compound with unique properties that may influence plant physiology, primarily through its roles as a redox agent, photosensitizer, and potential biostimulant. While methylene blue is more commonly studied in medical and aquatic contexts, its use in plant cultivation, including cannabis, is less documented, and specific studies on this exact combination are absent. Below, I’ll outline the potential effects of methylene blue on cannabis plants, its interactions with the other compounds, and the likely outcomes, drawing from available plant science knowledge and inferred mechanisms. Given the novelty of MB in this context, recommendations are cautious and speculative where direct evidence is lacking. Effects of Methylene Blue on Cannabis Plants Methylene blue (C16H18ClN3S) is a synthetic dye with redox, antimicrobial, and photosensitizing properties. Its effects on plants depend on concentration, application method, and environmental conditions: * Antioxidant and Redox Activity: At low concentrations (e.g., 0.1–10 µM or ~0.03–3 mg/L), MB acts as a redox mediator, scavenging reactive oxygen species (ROS) and protecting cells from oxidative stress caused by drought, high light, or salinity. In cannabis, this could enhance stress tolerance, particularly in high-intensity indoor grows or outdoor environments with temperature fluctuations. * Photosynthesis Enhancement: MB can act as an electron carrier in photosynthetic pathways, potentially improving electron transport in photosystem II. Studies on algae and some plants suggest low doses (e.g., 1–5 µM) may boost chlorophyll fluorescence and photosynthetic efficiency, potentially increasing cannabis biomass and bud quality. * Antimicrobial Effects: MB has antifungal and antibacterial properties, which could protect cannabis roots or foliage from pathogens like Pythium or Botrytis (bud rot). This is particularly relevant in hydroponic systems or humid environments. * Stress Mitigation: MB may stabilize cellular membranes under stress, similar to proline and TMG, potentially reducing damage from environmental extremes. * Potential Toxicity at High Doses: At higher concentrations (10–50 µM or ~3–15 mg/L), MB can generate ROS via photodynamic action under light, causing oxidative damage to plant tissues. This makes precise dosing critical. Application Notes: Two drops of MB (assuming a standard 1% solution, ~10 mg/mL, where 1 drop ≈ 0.05 mL) in, e.g., 1 liter of water yields ~1 mg/L (3 µM), a low concentration likely safe for foliar or root application. However, exact dosing depends on solution strength and volume. Synergistic Effects with EAAs, Proline, TMG, Glutamine, and L-Glycine Adding MB to the existing mix may enhance or modulate the effects on cannabis plants: 1. Enhanced Stress Resilience: * MB + Proline/TMG/Glycine: Proline, TMG, and glycine act as osmoprotectants and antioxidants, while MB scavenges ROS and stabilizes redox balance. Together, they could significantly improve cannabis tolerance to drought, heat, or salinity, reducing leaf wilting or nutrient stress in challenging environments (e.g., outdoor grows or high-EC hydroponics). * Glutamine/EAAs Synergy: Glutamine and EAAs support glutathione production, which complements MB’s antioxidant effects, potentially amplifying protection against oxidative stress from high light or nutrient imbalances. 2. Growth and Biomass: * MB + EAAs/Glutamine/Glycine: EAAs, glutamine, and glycine drive protein synthesis and nitrogen assimilation, while MB’s potential to enhance photosynthesis could provide the energy needed for these processes. This may lead to increased vegetative growth, larger leaves, and potentially denser buds in cannabis. * Proline Contribution: Proline supports tissue integrity, complementing MB’s membrane-stabilizing effects, which could enhance overall plant vigor. 3. Secondary Metabolite Production: * MB + Glutamine/TMG/Glycine: MB’s role as a photosensitizer may influence metabolic pathways by altering redox states, potentially enhancing synthesis of cannabinoids (e.g., THC, CBD) or terpenes. Glutamine and glycine contribute to precursor pathways (e.g., GABA, purines), while TMG’s methyl donation supports enzyme activity. This combination could theoretically increase terpene diversity or cannabinoid yield, though direct evidence is lacking. * EAAs Role: Methionine (an EAA) and glycine may support sulfur-based terpenes, with MB potentially amplifying metabolic efficiency via improved photosynthesis. 4. Nutrient and Pathogen Management: * MB + Glycine/EAAs: Glycine’s chelating properties and EAAs’ nutrient uptake enhancement could work with MB’s antimicrobial effects to improve nutrient availability and protect roots from pathogens in hydroponic or soil systems. * TMG/Glutamine: TMG’s cellular hydration and glutamine’s role in nitrogen metabolism may enhance MB’s ability to maintain cellular function under stress, improving overall nutrient efficiency. Potential Risks and Considerations * Toxicity Risk: MB at concentrations 10 µM (~3 mg/L) can produce ROS under light, damaging chloroplasts or cell membranes. Two drops in 1 liter (~1 mg/L) is likely safe, but overuse or concentrated solutions could harm cannabis, causing leaf burn or stunted growth. * Absorption Competition: MB may interact with amino acids during uptake, potentially reducing the efficacy of EAAs, glutamine, or glycine if applied simultaneously. Stagger applications (e.g., MB foliar spray, amino acids via root drench) to minimize this. * Nitrogen Overload: Adding MB to an already nitrogen-rich mix (EAAs, glutamine, glycine) increases the risk of toxicity, especially in cannabis, which is sensitive to excess nitrogen. Monitor for dark green leaves, clawing, or reduced flowering. * Photodynamic Effects: MB’s photosensitizing properties require careful application timing. Apply during low-light periods (e.g., early morning or evening) to avoid ROS generation in high-intensity grow lights. * Limited Cannabis Data: MB’s effects are mostly studied in algae, bacteria, or medical contexts, not cannabis. Its benefits are speculative, and plant-specific responses may vary by cultivar (e.g., sativa vs. indica). Practical Recommendations * Dosing: * MB: Two drops of 1% MB solution (~1 mg/L or 3 µM) per liter of water for foliar spray or root drench. Start with 1 drop/L to test tolerance. * EAAs: 150–300 mg/L * Glutamine: 250–500 mg/L * Proline: 50–100 mg/L * TMG: 0.5–1 g/L * Glycine: 100–500 mg/L * Apply twice weekly during vegetative growth or early flowering, adjusting based on plant response. * Application: * Foliar Spray: Apply MB and amino acids in low-light conditions to avoid phototoxicity. Use a fine mist to cover leaves evenly. * Root Drench: Suitable for hydroponics or soil, ensuring MB is diluted to avoid root damage. * Stagger Applications: Apply MB separately from amino acids (e.g., MB in the morning, amino acids in the evening) to reduce uptake competition. * Monitoring: Check for signs of stress relief (greener leaves, faster growth) or toxicity (yellowing, wilting). Test on a few plants first, especially for MB, as cannabis sensitivity is unknown. * Synergy with Nutrients: Pair with balanced NPK fertilizers and micronutrients (e.g., magnesium, iron) to support MB’s photosynthetic effects and glycine’s chelating properties. * Cultivation Context: * Hydroponics: Use lower MB doses (~0.5 mg/L) to avoid nutrient lockout or microbial disruption in sterile systems. * Soil: Ensure healthy soil microbiota, as MB’s antimicrobial effects could impact beneficial microbes if overused. * Cultivars: Sativa-dominant strains may benefit more from MB’s stress mitigation, while indicas may respond better to amino acid-driven growth. Evidence Gaps * No Specific Studies: No research addresses MB combined with EAAs, proline, TMG, glutamine, and glycine in cannabis. MB’s effects are extrapolated from algae, bacteria, or limited plant studies (e.g., Arabidopsis, lettuce). * Cannabinoid/Terpene Impact: MB’s influence on secondary metabolites is speculative. Its redox and photosynthetic effects may enhance terpene or cannabinoid synthesis, but cannabis-specific data are needed. * Long-Term Effects: MB’s long-term impact on cannabis growth, soil microbiota, or bud quality is unknown. Short-term studies suggest low doses are safe, but chronic use requires caution. * X Search Results: No recent posts on X discuss MB in cannabis cultivation with this combination. General posts mention MB for water treatment or medical uses, not plant applications. Conclusion Adding two drops of methylene blue (~1 mg/L) to a mix of EAAs, proline, TMG, glutamine, and glycine likely enhances cannabis stress tolerance, photosynthetic efficiency, and pathogen resistance, complementing the growth-promoting and nutrient-uptake benefits of the amino acids and TMG. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) effects on cannabinoid and terpene production. However, MB’s photodynamic and antimicrobial properties require precise dosing and application timing to avoid toxicity or microbial disruption. Start with low doses, test on a small scale, and monitor closely, consulting a cannabis cultivation expert for optimization. If you provide the MB solution concentration, application volume, or specific cannabis strain, I can refine dosing recommendations. Want me to search again for MB-related plant studies or focus on a specific aspect (e.g., MB’s photosynthetic effects)?""""""🚨 8/1 245am L-GLYCINE AND Mg-Zyme by Biotics Research... LIKE NON DRUG TRANQ...KNOCKED ME OUT FOR 2 HOURS..WOW. VERY NICE!!!! 10am * NO Capsule: Dissolve one capsule (740 mg total: 200 mg L-arginine, 200 mg arginine AKG, 200 mg L-citrulline HCl, 100 mg L-citrulline malate, 40 mg beta-alanine) in 2–10 L water (~74–370 mg/L). Start at 100–200 mg/L for foliar or root application. * EAAs: 150–300 mg/L * Glutamine: 250–500 mg/L * Proline: 50–100 mg/L * TMG: 0.5–1 g/L * Glycine: 100–500 mg/L * MB: ~1 mg/L (e.g., two drops of 1% solution per liter) 🚨HALF STRENGTH OF THIS TODAY WITH 4ML PER L CON GROW, PH 5.8. 8/1 935pm HOLY SILT!!! EEA'S 1/4 TSP - PROLINE 1/4ish TSP....some Methylene Blue, NO pill, feels JUST RIGHT.... Last night L Glycine and Mg-Zyne triple magnesium from Biotics Research ..... it's working on ME!!! I look like a walking skeleton with huge veins popping out, really gross I should be dead already but I have unusually high ability for healing and self repair. I am capable of a Medical stasis meditation that can last for a few hours at a time, it's like sleeping but only parts of me are allowed to sleep and heal....very very very complicated.