Yellow spots can be very normal on the first true sets of fan leaves. However, from the picture you posted I noticed your medium (not sure what type of soil though) is completely dampened. The little girls that aren't growing and the yellowing may simply be a symptom of over-watering. There is no root system to take up such large amounts of water and the system can/will be drowned out. If she's drowning, she won't be picking up the nutrients she needs from the soil, nor will that root system be able to properly establish itself. This yellowing could easily be the symptom of this over-watering and subsequent deficiencies rearing their ugly head.
Highly suggest localized watering for the first 2-3 weeks. Only enough to feed the plant without saturating the entire medium. Once saturated, it can take a very long time to dissipate that extra water. I also suggest allowing a proper dry out period so the roots can start to breath (not completely dry, though). As you continue localized watering, the damp soil surrounding her should start to give up moisture and dry out in time. When you enter week 3-4 (pending on growth patterns and root system) I would start doing full feeds w/ 10-15% run-off. Until then, localized is the way to go. From personal experience, over-watering early on can cause serious problems, including those possible deficiencies you see.
I do hope this helped, but if I'm missing something in terms of grow details, drop me a line in PM and we can discuss this further. Cheers and happy trails my friend👍