Stinkin Bishop - Tropical Winter

Stinkin Bishop Winter Grow Out Door in 11/13 hours Tropic Pythium attack! Lost two seedlings. Probable causes: Wet soil - Checked, my soil is wet. High humidity - Checked, 90% during gemination and there were outside. Nitrogen in the soil - Checked, my soil is fertilized with Earth Worm Casting that was fed by manure. Spores source - Checked, outdoor grow with mold in the lawn and plant containers. Recycled soil also. Documenting germination: Good start: Germination day 1 I popped all three seeds. I dilute one part of hydrogen peroxide (3% solution) with four-parts of very warm water. This speeds up germination and kills any fungus from seeds. After, 12 hours seed cover cracked as usual. And after 24 hours, root tip show on each seed. Germination day 2 I put seeds on flat cotton pads and align root to point in the same way. Used flat cotton pad. Sandwich the seeds. Wet the patch. Then added two extra layer on top. And another two layers on the bottom so the pads are moist not soaked. Put the cotton pad sandwich in a small plastic bag and prop it up vertically. After 24 hours, roots are about 3 mm long. I transplanted to soil with bean part popped up. Then the trouble spots: Germination day 3 I put seeds in soil. Soil was recycled from pervious grow. I then added fresh earth worm casting. Not really necessary but I does get the seedling to grow faster. Germination day 4 Bean and leaves survived okay. Germination day 5 Two seedlings damped off and died from rotted roots. Under the microscope, mold looks like Pythium. All sort of things could have been done to prevent it. Fungi likes Trichoderma, Mycorrhiza could have increase the survival rate. Bacterial likes Bacillus subtilis could get the job done as well. Next round, I should ferment my soil with Trichoderma and Mycorrhiza before placing seed. I believe that my soil already has microbes. I I don’t added beneficial living stuff during seedling. However, it would make more sense to add very early. Mycorrhizae grows with roots development. Adding early mean it takes a lot less. And adding Trichoderma saves a lot of head ache. Seedling does not need fertilizer. I still like to use earth worm casting to improve soil condition. To improve against damping off, I should make my mix lighter. And I should start seeds in a dry environment like in my lighted grow case. Oh well, next time. Germination day 6. One plant barely survive to show leaves. And her tap root was partly damaged. The plant popped out of the soil. And I dipped the stem into Trichoderma and buried her back in. With such a rough start and will be slow for the first week.