female pollen

1:5.67 dilution would be 3mM ... mistyped there. from "20 mM" to 2 or 3 mM ... just basic math. I'd suggest ~3mM target. If error of equipment makes it fall a bit short, not a problem. do need a 'milligram' scale if you want to make smaller batches. .001g resolution with +/- .003g error. 15-20 usd. they are cheap.

Definitely make your own. for ~20 dollars you can have enough STS for the next 10-20 years, lol. Don't buy the pre-packaged stuff. They don't adhere to basic storage and expiration dates and will sell you old reagents due to their ignornace - the mendo20 something or other brand did a few years back. silver nitrate used to be 2 usd per gram -- This may be higher now? sodium thiosulfate is 8 usd for 113grams (1/4 oz) bottle. -- "Anhydrous" need a roughly 1:4 ratio... if not anhydrous, need 1:6. Source for reaction instructions: https://www.merckmillipore.com/AE/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/silver-thiosulfate These instructions produce a .02 M concentration. You need ~3 millimolar concentration. 1 ml of this solution with 9ml of distilled water would be 2 mM. 1mL:5.567mL would be 3mM. Easy peasy. 1 application every 5 days, starting on first cycle of 12/12. 3 applications in total.

Hi, my friend. Why don't you do it yourself? I recently did the same thing, and with a bit of care and patience, you can do it too. I used a Silver Thiosulfate (STS) solution, and with consistent applications I was able to produce feminized pollen successfully. I recently completed this project, and you can check out the results I'm currently testing on my page. If you have any questions, feel free to ask. I'd be happy to help. 💚🙏👽