Dolato is looking pretty good didn’t expect a freebie to do so well lol. She is still maturing and trichomes seem to have little ways to ripen so she might need another week or two before harvest. Her scent is not so unique more strong but it’s there, sweet and creamy! Was expecting her to be ready sooner so I’ve been depleting her of nutrients two weeks now. Hopefully she speed up so I can harvest sooner since I’m using the tent for drying with clones, will need to use flowering tent to dry them all together.

Well we finally reached the end. The last week was more cruise control. Just trying to remove the big fan leaves that's blocking any flowers. The trichomes looking kinda ready but still acceptable. During the last few days I maintained a daytime Humidity of around 60 and nighttime of 55. Temperatures are between 73 degrees and 67 degrees. Two days before chop they got 48 hrs of dark and 3 days of no water to add to the stress. After the 48hrs darkness all the ladies Came out extra frosty. Loving the change of hues on the blue gelato. The vanilla Kush is very oily and very very strong smell. Not a strong smell coming off the OrangeGelato though. The green gelato has a creamy smell.

Eccoci di nuovo qui!!! Super eccitato per questa nuova collab con Kannabia Seed Company, team davvero al top, che mi ha dato l’opportunità di testare questa nuova genetica e di condividere i progressi con tutti voi!!! Come sempre partiamo nei bicchieri per poi travasare.. Questa volta verrà svolto tutto sotto la Lumatek Zeus 465 ProC, mi aspetto molto da questo ciclo!! WOOOOOW!! Pianta DEVASTANTE!! Grazie a tutti per il supporto ❤️🍀🔥

Was doing a slurry test and discovered springtails. Prolly have thousands and thousands of them in my coco. Not anymore...CannControl soil drench to the rescue! Saw some fungus gnat larvae crawling around too. Wtf. Hopefully the drench will knock them out too....at least the springtails are gone.

Everything is looking great just small buds on the Orion, quick one. nd royal dwarf is just getting started. But they look and smell fantastic. Northern lights is stacking up! Runtz too. Will be harvesting Orion in about a week

Microscope Trichomes observation checking amber

Esta semana la dejamos más tranquila a la Jack solo le hicimos un trasplante a 5 litros quedo bien , la dejamos recuperar unos días y después le hacemos las últimas podas y ya va a estar lista para florecer

Day 12 I lowered the light to 19 inches or 51 centimeter. It's on 50 % I started giving roots nutrients 2 days ago I guess they are doing good? Soon i will be slowly giving some grow feeding with micro nutrients I will add another video to this week in a few days Day 15 : Yesterday on day 14 i put the output on 75 % and lowered the light to 50 cm. They seem to like it but for the amount of plant i have i need to put it to 100% and i willl put to 55 cm from the plants. For cover more area. And will start with some lst training today. Untill the next week! Or around day 21. Peace! *** i added a day 19 to this week so u can see the difference in just 3 days.

We had a great growth this week, there was a big growth from the 24th to the 27th, because I watered on the 24th. I also thought that she has a little lack of magnesium in the bottom leaves and unfortunately I'm being Magnesium Nutrient.

First week of flower is in the books. All 3 plants stretched about 6" and grew tons of new branched. Really starting to thicken out. Been battling a more problem with sns217 sprayed at beginning of night cycle. 1 confirmed female while it's still to early for the other 2 although they look female. Only problem now is I'm starting to run out of height so hopefully they don't have too much more stretch in them. From here on I will be doing individual weekly updates on each plant they will be labeled AH1, AH2, and AH3 AH1 30" topped once biggest and strongest plant Female AH2 26" Kind of a boring one 1 main stem not much other growth AH3 22" The mutant! Probably On of the craziest plants I've grown. Has 3 main colas and a bunch of strange leaves with no training. Bushiest of the group Had a 4th AH that was only about 18" and struggling confirmed male and chopped 10 days into flower

7/4/2021 - Day 43 - pistils coming in, and she’s bulking up. Had her in a tent for a few days due to shit weather but we’re back in action outside. 7/7/2021 - Day 45 - I added a few pics. she's just doing her thing. I just keep coming out every day and moving things around LSTing probably for another week or 2. There are a few nibblets on a couple of leaves but I'm done with the neem oil now that she's flowering. 7/10/2021 - Day 48 - the net is doing wonders for LST. Some branches are starting the stretch, but I’m going to do some more LST until I start seeing actual buds forming.

4th week done, have some kind of deficiency that I'm not 100% sure on. Keeping an eye on PH more and making sure it stays a little higher. Started adding silicone as heavy buds was starting to make colas lean

Este sueño es posible gracias a Royal Queen Seeds, Marshydro, XpertNutrients y Trolmaster, sin ellos esto no se podria cumplir . 💐🍁 Dynamite Diesel: Royal Queen Seeds se ha asociado con Tyson 2.0 para ofrecer la genética desarrollada por este campeón en forma de semillas. La Dynamite Diesel recibe su nombre en honor al apodo de Mike Tyson al inicio de su carrera, Kid Dynamite. Es un cruce entre dos variedades míticas, la Sour Diesel y la Skywalker OG, que son conocidas por sus intensos sabores y sus efectos estimulantes,tiene un 22% de THC, lo que la sitúa en la categoría de peso crucero. En interior, esta variedad crece a alturas de 100-150cm y produce hasta 550g/m² tras una fase de floración de unas 9 semanas. Al aire libre, puede alcanzar hasta 2m de altura y produce una cosecha máxima de 675g por planta. 🚀 Consigue aqui tus semillas: https://www.royalqueenseeds.es/rqs-semillas-cannabis-tyson/664-dynamite-diesel.html 💡TS-3000 + TS-1000: se usaran dos de las lámparas de la serie TS de Marshydro, para cubrir todas las necesidades de las plantas durante el ciclo de cultivo, uso las dos lámparas en floracion para llegar a toda la carpa de 1.50 x 1.50 x 1.80. https://marshydro.eu/products/mars-hydro-ts-3000-led-grow-light/ 🏠 : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit 🌬️💨 Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ 💻 Trolmaster Tent-X TCS-1 como controlador de luz, optimiza tu cultivo con la última tecnología del mercado, desde donde puedes controlar todos los parametros. https://www.trolmaster.com/Products/Details/TCS-1 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 3: Ha sido una buena semana, ella ha dado un gran cambio en su lugar definitivo 😎. Se le ha aplicado un tratamiento insecticida con agua + tierra de diatomeas ( 1 cucharadita por litro de agua), también se le aplica un tratamiento fungicida con una infusión de cola de caballo para evitar futuro moho. A partir de ahora se riega manualmente con las dosis recomendadas por el fabricante.

We are at week 3 now. A lot of development, i have not used liquid nutes this week, it is raining a lot so no water needed, thus no liquid nutrients. I have top feed of dry nutrients mix, basically a mixture of a super soil recipe that usually is used within to make a hot soil: I mix all tue ingredients together and use it gradually about 40g as a powder, in the top of the soil. - Azomite 500g - Natural fosfate 500g - Biochar 1kg - Bokashi 1kg -Kelp meal 500g -Mamona meal 1kg -chicken meal 1kg -Potassium silicate 500g -Gypsum farm 500g -Diatomaceous earth 500g Mix all keep it in a soil bag, this pure mix and use it once every month. The companion plants have emerged and will protect the soil humidity mainly.

Week 4! - Still on track! So far so good. I repotted both plants into their final pots and filled them up with canna coco. Since the weather has been warm and sunny they have grown exponentially and have no signs of slowing down. Watering has been more frequent this week as it's been warmer but I have the feeling that I will need to water slightly less now that they have been repotted in the Canna Coco - this soil seems to hold the moisture alot more. I inserted some small bamboo sticks for support which seem to be doing the trick nicely, no problems. Any tips or advice going forward would be appreciated. Still pretty hands off and no feed/nutrients have been added - just good ol water.

Hallo zusammen 🤙. Sie wachsen sehr schön und machen keine Probleme.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.