2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

The girls are moving along nicely just watering with water ph to 6.4 this week as I top dressed with Gaia green 4-4-4.Spider farmer Se7000 dialed in at 30 percent and to the ceiling.Noticed some minor deficiencies Day 12 and check soil slurry 50/50 soil/water and the ppm was at 780 lil high at this early stage and the culprit.Going to work on lowering that a bit this week.I did add a top layer of fox farm ocean forest as well which runs a little hot with nutrients rather than the pro mix.I have to remember with these beautiful auto flowers less is better :)

Last week of flowering for our Apricot from fastbuds 420 This week only water was given to the girls, as at the end of week we are harvesting the plants! Very nice smells comes out of the tent, cant wait to try all of the fastbuds strains i have in the tent 🤗🤗🤗

Bene amici growers come potete vedere la fioritura procede senza molti problemi. Ho innaffiato le piante per la prima volta con una soluzione adatta alla fioritura e ora staremo a vedere come pian piano cominceranno a gonfiarsi i nostri amati fiori. Le piante iniziano ad emanare un profumo inebriante, le Durban posion stanno già assumendo un colore violaceo mentre le critical sembrano un po' più in dietro. Vi ringrazio anticipatamente nel caso lasciate un like!!

2ª Settimana di Fioritura 💐: Buongiorno amici della CampaCavallo! Grandi sorprese in questa settimana! 😶‍🌫️😵‍💫😵

22 days since my little girl sprouted from the seed🌱 The third week of growth of my Break-up Cake Auto 🥧💚 Nutrients: Plagron and Biobizz 💓💚: This week I am applying the classic root stimulator Plagron Power Roots: Plagron Roots root stimulator is a biological root stimulator that supports the formation of the root system. It contains 7.2% humic acids, 3% kelp (a beneficial extract of brown seaweed), 3% metabolites and 0.3% thiamine (vitamin B). It acts not only on the roots, but also on the entire plant. It increases the plant's defenses and is absorbed very quickly. 🍃🍃 ---------------------------------------------------------------------- BioBizz Bio Grow is a 100% organic, but also vegan-friendly fertilizer with BIO certification. Designed for the growth phase. Supports soil microorganisms and is ideal for use in all types of soil substrates and coco 💩💚 ------------------------------------------ Plagron Vita Race (Phyt-Amin) - is an organic foliar nutrient rich in iron that improves growth, photosynthesis and plant resistance. It is applied as a spray on the leaves during the growth and early flowering phase 💩🌱 ---------------------------------------------------------------------------------------------------------- BioBizz Calmag is a 100% organic, but also vegan-friendly fertilizer with BIO certification. Biobizz Calmag was designed for professional growers and home growers who need an extra dose of calcium and magnesium. It contains the addition of humic acid 💩☘️- I only apply it once a week. ------------------------------------------------------- Biobizz Bio pH-/ Biobizz Bio pH+ is a 100% natural and vegan-friendly pH regulator. Suitable for all types of substrates and crop types. For use in growth and flowering. 💩🍋🌱 ------------------------------------------------------- PRO-MIX CONNECT MYCORRHIZAL POWDER - in the substrate Generally: Break-up Cake Auto 🥧💚 The plant is already in pre-flowering 🌺, but flowering has not yet begun, so this week I am still giving nutrients for vegetative growth. However, this week - the third week - in most cases it does LST and defoliation twice 🍃 🌿 I think the plant is slower because it is growing poorly, anyway it was able to cope and in the end it is a strong, sturdy and beautiful plant, even if it is different. I am very proud of it 💚💪👍 I use the new PH and EC meter Aqua Master Tools P110 PRO, the measurement is fast and accurate. So far I manage to keep the PH and EC as they should be. I use Biobizz Bio +/- for correction. These are excellent products. I think that the plants definitely have a very good effect of spraying with Vita-Race and the excellent PRO-MIX Connect Mycorrhizal powder for root support. All the girls have been very strong and stable since they were little 💚 Training: As I mentioned above, I did the first LST and defoliation, the plants usually quickly re-leaf, other branches also grow quickly. So in this third week I do two defoliations and two LSTs 🍃🌿 This plant is a little different, but I still did a very good LST and the plant is now making two main buds 🌺 The girls are growing in a tent: Mammoth Pro+ 80, with reinforced reflective Mylar 600D foil Lighting: Mars Hydro TS1000, this light is sufficient for lighting in my growing space. The plants respond effectively and the light is technically very well managed - I try to have 50cm between the light and the plants, so I regularly move it up. Mars Hydro TS1000 Quantum Board 💡💡💡 Power: 148W Dimensions: 90 x 90 cm Dimensions: 360 x 330 x 34 mm Chip brand: Epistar Thanks for the likes See you soon 😍😻

The budlets have fully developed and are now starting to fatten up. There hasn't been any deformed leafs or signs of nutes burn so the flush of distilled water from last week seemed to work so I'll b using 1/4 the the recommended dosage for there first feeding this week. Ap is really showing her purple color with the budlets and her sugar leafs. Blueberry is more potent smell so we will see what this week has in store.

Yoooo :) The ladies are slowly eating what's left in the leaves. They got a dose of Final Solution and now only water. The harvest will be a little earlier due to mold problems. The terpinator + bloom stimulator did the job, the girls are covered in oily resin like never before :) Purple Punch, Cannatonic and Glookies already in the darkness before cutting :) Definitely you should check these lamps because they work amazing - https://bilberry.pl/en Older ladys bloom 62 day Younger ladys bloom 55 Caramelo bloom 50 day Cheers :)

Cosecha Reporte de Peso húmedo & Peso Seco de LemonPaya by FastBuds! ⚖️ Total peso húmedo 186 ⚖️ Total peso seco TBD 💦Pérdida de H2O durante el proceso de secado TBD 🎨Colores Un color verde claro brillante! 👃Olores 💐Floración Papaya (predominante) con un toque (potente) de delicioso limón 🏜️ Secado TBD 🍗 Curado TBD 🍽️Sabor A definir cuando esté bien curada en el reporte de humo Variedad 💪Resistencia Resistente 9/10! - Floración detenida por 10 días por el bloqueo de nutrientes que se ha presentado en la mayoría de plantas de este ciclo. Pero ella se recuperó muy bien y ha hecho unos cogollos densos al final de floración! 🏋️‍♂️Entrenamiento: Se ha adaptado bien al topping (en 2 ocasiones), supercropping y LST! Una crack! 🌱Vegetativo: Un vegetativo sin incidencias, con una bonita estructura! Ya en vegetativo al frotar las ramas, se desprendía olor a Papaya 😍 💐 Floración: Cogollos muy densos a pesar del bloqueo de nutrientes que superó sin problemas en 10 días! El olor a Papaya y fondo de limón es impresionante! Genética: Estupendo trabajo de breeding de FastBuds! 👨‍⚖️Conclusión: Una cepa deliciosa a plantar una vez en la vida! Muy interesante el perfil de Papaya y Limón!

Terza settimana di fioritura inoltrata..andiamo più che bene con lo sviluppo.buona genetica anche questa papaia cookie...bravi 👏 fast buds. Continuate così .,💪✌️

Simplemente hermosos pistilos naranja y un aroma exquisito 💚

She could have gone another few weeks believe it or not, but I decided to chop because I wanted something to smoke and the cluttered tent was annoying me.

She is smelling so strong and stacking nicely, the buds are dense and super shiny and covered in tricombs. So far there’s more cloudy than clear but no Amber yet will keep y’all updated :)

Day 50- All looking quiet good so far, no complaints. Fairly easy grow once you set up your plants training in veg. Only minor defoliation here and there when needed to expose bud sites to the light. Happy with the girls at the moment.

You gnat to do what you gnat to do.

Last week of the apple fritter.. As Im writing this I already harvested it. Ill report back in 2 weeks after the drying and before the curing. This was an amazing journey that its not yet finished. Drying and curing is very important and ill be doing the following: Drying Environment: - Temperature: Maintain between 18-22°C (65-72°F). This prevents terpenes from degrading while slowing the drying process. - Humidity: Aim for 50-60% relative humidity to avoid mold and overly crispy buds. - Airflow: Use a small fan to keep air circulating, but don’t let it blow directly on the buds to avoid uneven drying. - Darkness: Keep the tent as dark as possible. Light can degrade cannabinoids like THC. - Timeframe: Drying typically takes 7-14 days. Stems should snap (not bend) when drying is complete. Curing: - Jar Storage: Once the buds are dry, ill trim them again properly - Humidity Packs: Using Boveda packs (58-62%) to maintain proper curing humidity inside jars. - Burping: For the first 2 weeks, open jars for 10-15 minutes daily to release excess moisture and allow fresh air in. Afterward, reduce burping to once a week for long-term storage. - Curing Time: At least 2 weeks minimum.. Ill comeback soon with final dry weight. Wet was 400g. Keep up the good work galera 💜

She didn't stretch last week as I was LST training. We're starting the week at 13.5 inches. Enjoy.

HulkBerry placed 2 days in dark * Check the trichomes for amber * After first amber trichomes switch off the lights, leave it in the dark for 2 days * Watering 2l every 3 days * Always tuck in the leaves to expose lower tops

Day 49: Watered each plant with 1L with nuts 1568 ppm, 3345 us/cm, 3.3 EC (purple punch, strawberry banana, wedding Cheesecake) 1573 ppm, 3346 us/cm, 3.3 EC It wasn't me who did the feeding (gorilla cookies) 2 different feedings for the 10 plants Day 51: Watered each plant with 1L with nuts 1562 ppm, 3325 us/cm, 3.3 EC (purple punch, strawberry banana, wedding Cheesecake) 1514 ppm, 3293 us/cm, 3.2 EC It wasn't me who did the feeding (gorilla cookies) 2 different feedings for the 10 plants Day 53: Watered each plant with 1L with nuts 1632 ppm, 3472 us/cm, 3.4 EC (purple punch, strawberry banana, wedding Cheesecake) ??? ppm, ??? us/cm, ??? EC It wasn't me who did the feeding (gorilla cookies) 2 different feedings for the 10 plants Day 55: Watered each plant with 1L with nuts 1655 ppm, 3521 us/cm, 3.5 EC (purple punch, strawberry banana, wedding Cheesecake) 2142 ppm, 4557 us/cm, 4.5 EC ? It wasn't me who did the feeding (gorilla cookies) 2 different feedings for the 10 plants

Drunken Bitch Slap has been growing great bbn under the Hortibloom Solux 350. I just changed her solution today. She is in the midst of bulking now. Super frosty and sticky buds. It looks like vg it is gonna be a excellent smoke. She probably has about 3 weeks left till harvest. Everything is going great. Thank you Hortibloom, and Aeque Genetics. 🤜🏻🤛🏻💪🏻❄️🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g