This week's started with another defoliation 😬 Flowers are slowly forming and she's gaining about 3 cm in height every day. Glad the little stretch I hoped for is happening. Leaves are hitting the tent sides but I've heard it's not a big issue. Day 53: she barely grew 1 cm this night. I'm switching to bloom nutrients now (CANNA Aqua Flores), gotta give her food for those flowers. Day 56: the stretch is not finished at all, I guess it was just the beginning. The day after I switched nutrients I noticed dark purple spots on a couple of leaves and I still have no idea of what that is (toxicity, deficiency, fungus...?). The spots turned into necrosis and the EC was pretty high. Decided to step back a little and lower the EC again. It might be a P-K def., but more likely a nute burn instead. Those spots didn't spread luckily, so I'm trying to keep her healthy and looking forward to see those little buds fattening. Hopefully they won't become pop corn buds. Fingers crosssed and patience!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

05/20/2021 Wreak is putting on a glitter show the video does it no justice, sparkling is all I can say. She battled through PM break out and is doing well. Her smell is strong, i will be more descriptive later.....

As soon as I received these seeds I dropped them in a bowl of water @10am on April 30th. Planted directly into soil 7pm at sundown on the 30th. I wet the soil because it was dry. I prefer moist but settled for wet. The temperature was warm enough for germination week. The evenings were still lower 60's F, but the days warmed up above 70 °F. Night 1 it was dry with low 60's F Day 1 we had good sunshine and low 70's F. Day 2 We received a heavy rain in the afternoon and intermittently that night into the morning of the 3rd day (May 3) Our temps cooled off with the rain and we didn't get above 72° F. It was a little wetter than I prefer, but our soil drained well and fastbuds genetics can handle the less than perfect condition. Two popped on May 3rd (day 3) and the last popped on May 4th (day 4). The above listed nutrients were premixed into the soil at the listed rates per gallon of soil.

August 23: Just finishing up about 36 hours of darkness, then I'm going to wash the buds, then hang them. Harvesting a bit early due to time constraints but it looks good. The smell is very dank, not a tropical skittles smell, much more like wood glue as I've seen mentioned in other reviews, although I'm starting to occasionally catch a sweeter tropical scent as well. I wanted to leave it a little longer. There were still a lot of white hairs when I started the dark period, but a lot of them seem to have developed into swollen calyxes so I'm pretty happy. I was a little worried they were making seed pods after last time, but I broke one open and all that was inside was red hairs. I'm going to keep an eye on the RH while they're drying and get them into jars and try to wait as long as possible before I start sampling. I can tell this is one of those batches that's going to get better and better if left alone for a couple months. Just did the wash, did 1.25 cups of 34% food grade H202 and a squeezed lemon in a 5 gal bucket. Another bucket of just water to rinse. Then I had a fan on it while hanging in the sunlight from a skylight in the bathroom for 30 min, then put the fan further back and on oscillate, then turned it down an hour later. Left it in there for a few hours, then hung it in the grow tent with everything removed except an oscillating fan, pointed away from the buds. I have the whole plant hanging in the middle of the tent and the tent is holding at 60% rh. Next run I'm going to do 2 buckets and only top once. I might leave them a bit longer in veg too to maximize the height in my grow space, and I'll get a proper 500w bulb instead of continuing to run 1000w bulbs at 50% to lower the heat a bit. The weather will be cooler and I'll only run one air stone in each bucket which should help with res temp. I'll probably foil-wrap the buckets again as well. I should be able to get another run done by the end of the year. I have a White Widow fem seed I've been waiting to use but I'm not sure what else I want to grow yet. Maybe Granddaddy Purp. I'll take some micro pics of the trichomes this week and start curing, and I'll post a proper review in a couple weeks. Happy growing everyone! August 24: I took it out of the tent to inspect. No mold or mildew. The middle of some buds are still pretty wet. I'll keep an eye on it. Wet weight on the stem is 158 grams/ 5.5oz. I harvested a couple branches last week and hung them up. I just ground up one of the buds, left it overnight and vaped some today. There was a bit of Chemdawg Tangerine in the grinder already but overall I got a lot of thick vapour, instant effects, and a really great tropical taste. I busted out the microscope camera and I realized I had a couple buds that fell off before I washed the plant in the H202, and a couple buds that came off in the washing process. It doesn't look like washing the buds took off any of the trichomes. I do seem to find less debris in the buds, not that there was much there before. The trichs look great. Just a light toasting of amber. The washed bud actually seems more dry than the unwashed bud. August 25: there's a real chlorophyll smell coming from the drying room today, it was much terpier yesterday. Leaves are getting crunchy, stems are still fully bendy. I ended up taking it down in the evening. The drying room rh dropped from 60% to 55% and the buds were starting to look too dry based on my last grow. I put it all in a paper bag for the night and I'll put it in jars tomorrow morning. Broken down most of the way it came out to 48-49 grams. From the bud structure, I definitely feel like it would have benefitted from more grow time if I didn't have to travel. More to come. August 26 - the edges of the buds are getting really crispy and the buds are getting tighter. I checked the paper bag earlier and it was 65-70%. I got the impression it was time to put it into jars. I'll keep the lids off for a bit.

18/12 - Harvest Day! One is very big and the otther is a miniature...i would love to master this One day 🤣

1ml per Litter pk13/14 : they became chunky and denses

Graduated, and am off from work for a few weeks, so went overboard on photos. Plant is doing well, leaves are starting to turn yellow (strain trait). Can't wait for the harvest and being able legally gift my xmas ornaments to my cousins during our "cousins walk" at our family xmas party.

Einige Blätter, die ich nicht aus dem Weg bekommen habe und welche die künftigen Buds beschatten, habe ich abgenommen. Ebenso abgestorbenes Blattmaterial. 22.11. Die ersten Trichome sind sichtbar und darunter scheint es ziemlich violett zu sein *freu*

Scrog before preflowering. Add new photos 3 days more grow

Esta semana, nuestras plantas empiezan a mostrar las primeras flores. Ya tenemos la sala con las cuatro luminarias funcionando al 75% de su potencia y las he separado un poco más, ya que veo que con led, el espacio internodal es muy reducido. Añadimos a la mezcla de fertilizantes CALMAG de BioBizz, que aportará un extra de calcio y magnesio. Regamos cada tres días aproximadamente, una vez con fertilizantes y otra vez con agua. Comenzamos a reducir la humedad relativa en la sala y desconectamos la calefacción en fase nocturna, para que empiecen a pasar un poco de frío.

Hello everyone my 5x5 grow is starting and here's the details 🍻👍 7x 11l pruning pots/1x airport 10l Soil: Bio Bizz light mix Nutrients: Sensi Bloom A&B, Terpinator, overdrive, carbo voodoo juice and maybe some rhino skin Light: Spider Farmer 4000 Heard good things about it Tent: 5x5 BudBox Water supply: tap water I let sit for 24 hours Ventilation: 8ich ram fan was gonna get the stealth phresh but yeh got a pillow wrapped on it to reduce noise using 8inchto12inch carbon filter and a 16ich standing fan on day 19 I will be adding 2x co2 bags 🍻🍻🍻 that's basically everything oh my ph stays at 6.5 👍💪🍄 13th December 2019 All 8 seeds sprouted Plain water ph to 6.3 One of my watering was abit highPH as I didnt check the ph but hasn't done any harm my expectations for this grow realistically I should easy get 20oz of dence buds but I would be happy and content with 17oz but this light I'm using is the best I've ever used very expensive to me £500 for 1 light I know theres more expensive but this is a upgrade for me considering I was using Mars hydro 300 and cheap cheap China led my ak47 grow was done with really bad lights and it looks like it didn't yeild that bad I had to throw my BlackBerry kush and stardawg in the tent hope they dont take to much room lol ik these cookies are gonna grow nice and big under that light 🥂

Seems all is ok . Flowering 3 rd week. All of them are huge , ses.litighting case, distance, but only way to control hot air. P.S ACTUALLY WE WAS NOT EXPACTING CLONING OF AUTO FLOWERING STRAIN ,BUT IT HAPPENED, TOOTS ARE CRAZY . =)

Both plants are thriving. The mimosa still growing fast despite the fact it was burned by the light. The bourbon berry is trying to keep up with the mimosa plant.

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Start 3 weeks .14 days

This strain was easy to grow right from seed. I think it will be one of my favourite strains this grow. Time to dry and cure. I will report the weight when dry. 💚✌️🏼

Harvested a little early. But about 70% milky and some clear and dark. I have it drying now. About 50% humidity but it goes up and down. 181 grams from 3 plants in my 4X4 with a TSW2000. I didn't expect to get that much on my first try. This sour joker x sour ghost is the real deal. It's not hell's OG unreal but it's a nice smoke and gets me rocked.

Going into flowering is always a special moment and we prepare to do it in the best way to make the plant understand that it is time to become a woman and speed up the start of the formation of buds. We start with 24 hours of darkness this to give a strong break and sign of change to the plant that understands the change of time and prepares to go into flowering. Our Slurricane was worked with a topping x16 buds that worked really well, we have well aligned branches, here nothing escapes upwards. The small growing bruches are perfectly aligned and it is another fantastic characteristic of plants with low internodal distance. Another thing that gives the plant the signal of the start of flowering are the flowering stimulators we friends of Plagron use Power Buds. The start of the flowering program includes the same additives as the vegetative phase Power Roots, Sugar Royal, Pure Zym and adds the flowering stimulant Power Buds. Now the basic fertilizer is Alga Bloom specific for flowering. The shape is very very beautiful as the structure of this plant, I definitely love plants with short internodal distance. Elegant, to see, to photograph, to grow. Green Sensation will arrive as the hero of the end of flowering and at the same time it will be time to remove Power Roots and a week later the Pure Zym enzymes. It is recommended from the 4th week, if the plants flower fast I start at the 3rd depending on the size of the bud. Try a seed of this strain that drives us crazy... It's a super News! ---- https://www.zamnesia.io/it/11177-zamnesia-seeds-slurricane.html Zamnesia Description // Do you want to add a prestigious genetics to your grow room? Slurricane is a premium US strain created by In House Genetics and now available to growers thanks to the breeding processes implemented by Zamnesia. Born from the fusion of Purple Punch and Do-Si-Dos, Slurricane is 60% indica and incredibly vigorous. Whether grown indoors, outdoors or in a greenhouse, this strain can produce large quantities of dense and aromatic buds. Add Slurricane to your assortment and enjoy growing one of the most beloved hybrids in the United States. All the best that mother nature can offer is on ---- www.zamnesia.com