📆 Week 2, 13-19 May 2024 13-19 May - Observed and watched the seedling grow. 📑 Plant is strong and healthy. I see no problems in early development or the speed of growth. 🍶 13 May initial nutrient solution not changed, 1st change is week 3 🍽️ 13 May initial feeding schedule unchanged 💧 Using reverse osmosis water with EC/TDS at 0 🐉 Nutrient solution EC 1.0 at 72 degrees F 🔆 Light power at 50%, DLI 12-15 canopy coverage at 18hrs 😤 Using PYPABL, Air Pump, 400GPH That is it for this week. Thanks for the look, read and stopping by.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

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08/05 - Comenzo su primera semana en etapa de floración. Se encuentra perfecta sin ningun problema hasta el momento. Pase las luces a 12/12 y ademas subi un poco la potencia ya que entra en floración. Se encuentra muy tupida, los cortes que le realice funcionaron y se desarrolló muy bien. Ya se puede sentir un poco de aroma a planta. En estos dias estare publicando mas imagenes de como viene. Podes seguirme en Instagram como @bruweed_

👉Alrighty Then👈 63 days in flipped at 28 days 👉Halfway there 👈 It's been a great week all plants are killing it ......... 👉we are now in full flowering 👈 There all gonna get a slight defolation over the next few days to let air and light get in there which seems like I'm doing just about every feeding 👈 👉So I topped all but Babba Kush and Slurricane they didn't require it ... 👌 Got some nice level tops 👌 I've now started there full flowering nutrients program , so we are set to go ...... Persian Pie from Greenhouseseeds Full Gas from Greenhouseseeds Babba Kush from Greenhouseseeds Rainbow Melon from Fastbuds Papaya Sherbet from Fastbuds Weddingcheesecake FF From Fastbuds Purple Oreoz F1 From Seedsman Slurricane From Premium Cultivars Soil by Promix Nutrients by Cronks Well this should be fun 🙃 Thanks to all my growmies out there for stopping by its much appreciated 👈 👉Happy Growing👈

Busy week this week, both in the garden and in life, hence the later update than usual. Early week gave the girls a good leaf stripping on the inside of the plants. Also removed any scraggly growth that was not getting any sun. As usual really helped open them up to more airflow and light penetration. At start couldn't even see the sun through them! Next day both looked great with no visible signs of stress. Now I'll say it was a VERY hot week this week, daytime highs 32°C feeling like 42°C with 90% humidity and the girls were thirsty AF, dropping heavy every night, . Watering every evening, hose on full soaker setting for 3 minutes per plant, roughly 35 liters of water each! Even with all the water, the bigger plant was looking sad, and I had a sneaking suspicion that it just did not have enough root space. So I tuned into my inner MacGyver again, and came up with a solution. Using no nails or screws, I was able to fashion a 3x3 bed for her! All thanks to growing on plastic skids. Just slid 2x4 PT into the slots in a 3x3 square, and then slid 1/2" PT sheet (cut to size) on the inside of the wood. Afterwords cut the fabric pot, and lay it down to make the perfect base inside the new bed to prevent soil from coming out. Topped it up with three wheel barrow of garden soil that I had left from the spring load, watered and hoped my idea worked. The next day, she's looking happier than ever, and I haven't seen a droopy leaf on her since! Now she's really ready to bring on some serious bud weight. Mentioning that, I also added the second layer of horizontal trellis, as well as vertical trellis around the smaller plant. Temps look cooler for the next two weeks, and not much of any rain forecast, even though we desperately need it. At least with forecast being 25°C highs and 10°C lows the girls should slow down on water intake, trying to conserve where we can right now but got a keep them fed. Until next week!

All good

Week 31 december - 6 January 6/1 feeding day

Gelato and Northernlights are the tallest...Gelato showing signs of flowering..

Crazy stretch, amazing structure looking fwd to see some 🌲🌲🌲 🤟🤟🤟 GHL

Another week just passed. I have reduced the main flowering nutrient by 1ml, because I’ve introduced Plagron Green Sensation as an additional flowering nutrient. This thing increases your yield incredibly! If you start looking at bud formations starting this week, you will notice significant changes.

Would grow again. Top Tier strain. Very high yield but mainlining caused a lot of sugar leafs. It took some time to hand trim two plants, but it was worth the time and it will create excellent extraction. All buds are good and i would say a and b tier only. No larf Greenhouse op

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First week of flowering and it seems to go quite good, the brown powder we see on the leaves is insecticide. Next week we will start with some food!

Su 5ta semana completada ! (mas un día porque debí subirlo ayer) El crecimiento sigue progresando y esta semana aumente la dosis en un ml (la siguiente semana creo subiré a 4ml/L) ya que empecé a observar un poco de decolora miento de algunas hojas aunque también sobre fertilizar es algo que debo de tomar en cuenta. Seguiré haciendo LST quizá un par de sesiones más porque estoy seguro que no falta mucho para entrar a floración y espero establecer una buena estructura antes de ese momento para que empiece q crecer hacia arriba, espero salga bien; es mi primer LST 😃 Sigan este diario semana a semana 😃

🌱: 💧 : 8l 💡 : Dli: 40 mol/m²/d 🤔: