2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

10.10. Eine Woche unter greenception gc 4 unter 12 h. Irgendwie wie wellnes für die Pflanze. Photos folgen. 14.10. Durstig... Irgendwie, wien Vulkan kurz vor der Eruption? 15.10. Blaues Lichtspektrum aus. Effekte(eigene Erfahrungen) : Start des Spätherbst für die Pflanze. Bloom Reaktor started!

This marks the start of week 4 for these wonderful lady's . I'm going to be slowing up on the nutrients and the watering noticing some stuff I don't like seeing . For the growth difference in 1 week all I can say is WOW I'm getting very excited to see what the future holds for these young lady's !!! I'd like to thank every one for their continued support! I've learned so much in such a short amount of time because the community is KICK ASS . I'm going to be repotting these fine young lady's into their final homes at the end of this week! Stay tuned ladies and gentlemen alike and remember to check my diaries daily for updates ! Anyone checking out the pictures it isn't mites or any of that I been having a battle to get them to my standard of clean since the transplant. Slow and gentle is the way so no damages are done ! :) -Happy Growing!

Critical 28 day bloom 🌿 I didn't fertilize this time. ppm 350 2 weeks later overdrive

Geringer Wachstum.

This girl is wanting to go nothing but vertical. Bent her 90 degrees and it's paid off. I've been moving the main stem around the pot trying to encourage as many tops as possible. She is looking real bushy for a sativa. I don't suspect that will last long, 1 more week then flower time.

Had a bad humidity (spots on leave are wet spots that got handled ASAP) scare last week so got a dehumidifier up in there comfortably. Still smooth sailing.

she is in her last weeks ... we will see what she offers us ... with very little smell but nice ...

Calcium deficiency is rearing its head, it's only minor so shouldn't really be a problem.

Weather is getting a bit better this week. Almost no rain anymore and much more sun comparing to last week. Blueberry Ghost OG and Kali AK still don't want to start flowering, but they slowed down from growing taller at least, so there are hope of they to start flowering soon (hopefully).

She has entered senescence with mostly milky trichomes.. She should be ready to chop in a week or so..

Super dense already at 36 days of flower full on resin monster ...alot of thricomes ...looks like I found my first mother plant ..definitely running this beast again resin resin resin

Ola growmies! O gráfico dos nutrientes corresponde à alimentação do gorila real # 1 (a mais desenvolvida) foram misturados em 2,5L de água. (25/07/2021). Alimentação Royal Cheese 1 e 2 (4L): E.C 0.63 /pH 6.3 BioGrow - 3ml; BioBloom - 1.5ml; TopMax - 4 ml ; BioHeaven - 3 ml; Activera - 3ml; CalMag - 0.5 ml --------------------------------------------------------------------------------------------------------------- Alimentação Royal Gorilla 2 (adicionei pela primeira vez o BioBloom) (2L): E.C 0,60 /pH 6.3 BioGrow - 1.5 ml; BioBloom - 0.5 ml; TopMax - 0.8ml; BioHeaven - 1.5 ml; Activera - 1.5 ml; CalMag - 1.5ml - meti mais que nas outras por causa da sua aparencia estranha, não sei o que fazer para melhora-la.. 27/07/2021 - Quebrei outro ramo sem querer da RGorilla #2, o ramo era fraco mas tinha uma folha grande. 27/07/2021 - Retirei algumas folhas secas e amarelas, especialmente na Royal Gorilla 1 e na Royal Cheese 2 --------------------------------------------------------------------------------------------------------------------- 29/07/2021 - Alimentação Royal Gorilla #1 (2,5L): E.C = 1,02 ; pH - 6.3 - BioGrow - 2,8ml ; BioBloom - 2ml; TopMax - 2,5ml; BioHeaven - 3ml; Activera - 2ml; Calmag - 0,6ml Alimentação Royal Cheese 1 e 2 (4L): E.C = 0,84; pH = 6,2 : BioGrow - 4ml; BioBloom - 2,5ml; Topmax - 5ml; BioHeaven - 4ml; Activera - 4ml; calmag - 0,7ml Alimentação Royal Gorilla #2 (2L): E.C = 0,80; pH = 6,2 BioGrow - 1,8ml; BioBloom - 1ml; TMax - 1,5ml; BioHeaven - 2ml; Activera - 2ml; Calmag - 1,5ml ------------------------------------------------------------------------------------------------------------- 31/07/2021 - Tirei algumas cabeças pequenas que nao iam desenvolver e tirei mais umas folhas amarelas das quatro. (as mais secas)

D 41 Sounds good

This is how my ladies are looking at the end of Week 6 of Flower going into week 7, I have uploaded a video for you guys with all the information, any questions just ask away 👍🏾👊🏾😎

The Plants had a shorter vegetation period than i had the previous runs. I usually like to „fill up the tent“ mostly with more plant mass. But the timing wasn’t perfect. Im still overall happy with the current state. All organic. Living soil and RO water. And the occasional compost tea. 15l fabric pots wich aren’t ideal for living soil but still work out great so far. Hope you like it too

Sleepy Joe's are wide awake and throwing some nice flowers. Stacking up nice and starting to show some trichomes now. Odours in the room are very pleasant. Hoping these ladies hit their high tech levels. 34% is nothing to sneeze at.

It's been two weeks since i started using the UAP 1500 light, and the results are definitely showing! The 'Peyton' papaya seems to love it. The leaves have this amazing vibrant green, and the whole plant looks so healthy and strong. Lemon Cherry Runtz is looking fantastic! The buds are dense and frosty, with those beautiful purple hues peeking through. You can tell it's a high-quality grow. The aroma must be incredible – that sweet, citrusy smell with a hint of cherry. It's definitely living up to its name! The plant seems super healthy, and the trichome coverage is impressive. It looks like it's getting close to harvest time.

Olá a todos! Essa é uma planta que eu não estava dando tanta atenção.. mas resolvi agora a me comprometer melhor com ela e finalizar ela para obter o melhor rendimento possivel até o final, ela passou por um stress severo, ficou debilitada de nutrientes por negligencia, e começou a se recuperar, e agora ela está se desenvolvendo bem rapidamente, vejamos como vai ocorrer até o fim. ( 14/07 ) Espero levar ela por ainda mais uns 35 dias aproximadamente podendo se estender até 42