Week 7 - Flower 3 Sangria - by Twenty20 Mendocino 4Q 2022 Veg Week 4: Wednesday 01 February - Tuesday 07 February 2023 Start of Veg - 1 Node Start of Week: [ 2022-02-15, SA20 50:B3:7:1] End of Week: [ 2023-02-21, SA20 56:B3:7:7] HeightWeekStart: [ 10.5, in] HeightWeekEnd: [ TBD, in] Start Date: 28.12.2022 # Seeds Wet Harvest Window: 17 - 27 March 2023, 79 - 89 Days EnvironmentGermination - LightDistance: [ 14.5, in] - LightStrength: [911, 505, µmol/m2/s] - Temp: [ 79, 85, °F] - RH: [ 73, pct] - HumidifierTrigger: [ 69, %] Fertigation (BioAg, Mammoth-P) - [x] R&R/Recalibrate BluMat’s at next IDEAL pot moisture __________________________ Wednesday 15 February 2023 SA20 50:F:7:1 Thursday 16 February 2023 SA20 51:B3:7:2 Friday 17 February 2023 SA30 52:B3:7:3 - Blumat watered to 400 ml Runoff. Turned sensor knob 1 pt Tighter. - Nonetheless - plants look Awesome, and Saturday looked Better!!! Saturday 18 February 2023 SA20 53:B3:7:4  - Adding BioAg CytoPlus to Res - Ful-Power: [ 20, ml, gal], CytoPlus: [ 1.25, gm, gal], Photosynthesis Plus: [ 6, ml, gal], SLF-100: [ 5 ml, gal] Sunday 19 February 2023 SA20 54:B3:7:5 - After EMPTYING the Reservoir yesterday - Sangria is cranking her transpiration, pot is demonstrably lighter. We’re cooking w/ Gas!!!! - Added CytoPlus cited yesterday    Monday 20 February 2023 SA20 55:B3:7:6 - [x] Crank Light to Maximum Height & Reset CORNERS @500 PPFD (For full Light penetration) - Monitor TEMPs (May have to reduce to maintain cabinet temperature < 82 °F - [x] Monitor TIPS for Light Burn as we’re 200 PPFD Above Optimal CORNERS  CENTER  Tuesday 21 February 2023 SA20 56:B3:7:7 - 0800 Look, bleh - an hour later - happy. And it looks like SHE WAS WATERED AGAIN. - Basic goals - Raise Light to maximum, adjust light w/ Dimmer. Min Corners ~500, Max Center 1000, PPFD - [x] Adjust dimmer to nominal 500 PPFD in Corners - [x] Turn BluMat down 2 pts - [x] Raise light to top of Cabinet # I want it DRYER IN THE BOTTOM than BluMats current setting. Light DistanceCanopy: [ 15, in] Light DistanceCenter: [ 13, in] WaterAmt: [ 1350, ml, day]

All good

July 27: she has started preflowering already so I’m going to go with just phyto-forcing with a far red 730 nm light for a few seconds at dusk each night. This will be easier than putting them in the garage for 12 h a night for three weeks. This is a good trick for northern growers. July 30: doing great and seems to be flowering so all is good. Video shows how easy it is to make lazy compost tea which the plants definitely love. July 31: watered in early evening with compost tea. Aug 1: I’m a total tea convert now. Plants always love it.

Bonjour à tous, ces derniers jours ce sont bien passé pour mes petit bébés elles grandissent doucement mais sûrement.C’est la première fois que je travail avec cette gamme de produits (je vous est mis des photos des produits 😉) pour l’instant j’en suis satisfait après, il faut voir le résultat final ce qui est moins facile 😂. Mes petit bébés commence leurs 5ème semaine (je me suis trompé d’une semaine 😕 désolé 😉) trop pressé!!! 😂 de mettre de nouvelles photos 😜. Je vous dis à là semaine prochaine vivement 😉 bonne journée à tous. Je vous remercie 😉.

Vamos familia actualizamos la cosecha de las gelato Olandese de Dutchfem . La verdad que el secado muy bien 7 días en Malla y a los botes, 40% humedad y 24 grados es la temperatura ambiental que han tenido en el secado. Por lo demás de miedo os la recomiendo. Gracias a DutchFem, Agrobeta y Mars hydro , sin ellos este proyecto no sería igual 🙏. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Buenos humos.

This week I basically gave the certs, fixed the light intensity and got my humidity control system all dialed in. The plants have seen more growth than ever! The seedlings are definitely growing way better than the older plants so I'm not sure what to do now I'm regards to flowering all of them or focusing my energy on a few

I am very pleased with this harvest, my buds are fragrant and very strong, I did not use too much of them, it gives me the ability to concentrate and think highly, the aroma and taste of mango and lemon. I have come very far with it. So great. And happy grow.

2025-07-14 What should i say, aim kinda speechless. she stopped stretching but is still growing wider, buds swelling- iam sure she will need at least 4 more weeks.- shes huge and needs time to maturate. she started smelling the typical Mimosa scent. she got a topdressing with silicium flash- soon she will get some BIo PK 5/8 xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Mimosa Auto is a contemporary autoflower strain boasting potency and bud quality that rival photoperiod cannabis strains, with a staggering 21% THC content that delivers a remarkably strong high sure to bring a big smile to your face. Mimosa Auto is versatile, making it an excellent choice for various daytime activities. As for its flavor profile, as you might expect, it offers delightful citrus notes, featuring hints of orange, lime, and lemon, with a subtle touch of diesel. It's a flavor profile that's sure to satisfy even the most discerning cannabis connoisseurs! In indoor cultivation, Mimosa Auto reaches a manageable height of 60–150cm, while outdoor plants typically reach 80–160cm, making it suitable for tight spaces. Mimosa Auto doesn't keep you waiting: it yields its delectable harvest in just 10 weeks from germination, including 7–8 weeks of flowering. Indoors, you can expect up to 400g/m², and outdoors, it delivers up to 130g per plant. xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Growing Environment Setup & Specifications Lighting System Model: Sanlight EVO 5-150 Quantity: Up to 5 units Power Output: 320W per unit Maximum Total Output: 1600W Environmental Control Management Unit: Growcontrol Growbase Pro Humidity Management: Dedicated Humidifier Dedicated Dehumidifier Temperature Control: Primary: System-controlled Secondary: Additional Radiator for supplemental heating Air Management Air Circulation: 2x Stand Fans Air Extraction: Exhaust Fan with Filter System Irrigation System Primary: Autopot XXL Pot System Water Storage: Flexi Tank Root Zone Aeration: Airdomes Dedicated Air Pump Growing Medium Type: TERRA PRETA MJ-Mix Nutrients System: BIOTABS Method: "Just Add Water" Strategy Type: Organic-based feeding

Mi sono dovuto assentare perciò quando ritorno in sede indoor farò una desfogliata importante e le metterò poi a 12 ore Questa volta provo con 24 ore di buio,1 giorno a 11/13.. poi fisso l'orario classico 12/12..dicono che si velocizzi la fioritura... vedremo.. A vederle in un video sembra tutto a posto...😅

Day 70 – Flower Week 1 (Flip on Day 67) The plant has transitioned into flowering smoothly. The wide, low structure from the veg phase is still obvious—a dense, even canopy stretched neatly across the scrog net. Multiple tops are sitting at a uniform height, and the first small pistils are starting to show. Foliage remains mostly healthy: a rich mid-green with a slight sheen. A few of the older fan leaves down low are beginning to yellow lightly, which is normal at this stage as long as it doesn’t accelerate. New growth at the top looks vigorous, a sign that the root zone is active and stable. The post-flip stretch has started but remains moderate, which suits the flattened training style well. Light penetration across the canopy is excellent, giving every bud site an even start.

Overview of my grow: Tent: Secret Jardin DS120 4 x 4 (120 cm by 120 cm) Lights: 2 x 120 Watt CTlite c4 clusterled Climat: Trotec Dehumidifyer 240 watt- 10L/24h Filter: Prima Klima carbon filter PK2600 fan: 2 x Secret Jardin 20watt osc. fan Pots: Gronest 4 x 11 liter airpots Water : automated water system PH: bluelab Nutrients: Plagron cocos A + B Plagron PK 13/14 ATA cal/mag Epsom salt Week 10 the strawberry cheescake is very frosty it look really like a layer of snow on this plant. i started to flush her. very nice for a free seed. smeel is also amazing. i dont smell any strawberry yet but maybe it is in the taste. 😋

Sooo both of them are healthy had do drop down a bit of the nutes because of some burned tips around the plants, but other then that they are doing good, they are starting the bulk up slowly, but I think about 4-5 more weeks and its chop time!

Day 62-30/01/23 #1 harvested!!! Day 64-01/02/23 #2 harvested!!! Day 68-05/02/23 #3 harvested

Moved into main grow tent with other flowers much cooler environment, on 12 /12 light schedule now and feeding kelp based Dutch science nutrients bloom

Rolling along , no smell yet, she seems happy

Really surprised with how everyone turned out! Everyone is starting to flower but Kayla definitely got the lead in terms of pistils and bud sites. These ladies still need to be LST every other day. Looking forward to an early harvest!

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Welcome back!!! It’s the beginning of week four. I now consider this plant to have finished the seedling phase, now move onto the veg stage. THURSDAY - Day 22 10 October 24 - Switched the blue spec light out for the red spec light. - 380 PAR/ PPFD at 26 inches distance. - Topped the two bottom branches. - Started doing a little LST by adding a few staples and a little bit of branch manipulation. - She’s slowly shaping to my vision for this plant. - Gotta start thinking of up potting her soon into her final home. FRIDAY - Day 23 11 October 24 - Added two more LST staples. - Just a day under the lights. SATURDAY - Day 24 12 October 24 Watering day, 1 litre of dechlorinated tap water. - Base water 1 litre - 61.1 PPM Final - 1 litre at 6.45 PH at 62.8 PPM Runoff - N/A - Thinking of removing those giant fam leaves soon, they’re becoming cumbersome. - Did my final planned topping of the two top branches. - I tweaked a top branch and there was a pop at the knuckle, not a complete break but there’s a bit of damage. - I’m fairly confident that she’ll send resources to fix it and make it even stronger. - No stress, we push forwards and roll with the punches cause that’s what we do around here. SUNDAY - Day 25 13 October 24 - Just a day under the lights. - She doesn’t seem too bothered by yesterday’s branch tweaking. - There’s a but of a split but it seems to be scabbing over. - I’ll keep monitoring it over the next few days. MONDAY - Day 26 14 October 24 - Just a day under the lights. - Also a good recovery day. TUESDAY - Day 27 15 October 24 Transplant day into a 5 gallon pot and her final home. 60% Gaia Green Soil 20% Worm castings 20% Perlite 8 Tbsp Gaia Green All Purpose 4 Tbsp Gaia Green Power Bloom 5 Tbsp Diatomaceous Earth 1/2 Tbsp Dynomyco Amended this mix and transplanted. Watering day, 5 litres of dechlorinated tap water. - Base water 5 litres - 61.2 PPM - Add 2.5 ml Cal-Max - 172 PPM - Add 5 ml Supermax - 206 PPM - Add 15 ml Miicrobial Mass - 337 PPM Final - 5 litres at 6.61 PH at 337 PPM Runoff - N/A - Removed the LST staples to let her acclimate to her new home. - Will resume LST in a few days. - Removed the lowest fan leaves. - Some leaves are funky but they also are in the way of the important growth so they may get removed in the near future. WEDNESDAY - Day 28 16 October 24 - Just a day under the lights recovering from yesterday’s transplant and extended light hours. - She’s perked back up and looking happy though. - Probably resume with LST staples tomorrow. - The tweaked branch looks like it’s going to recover just fine, just a little weary about working it too soon. This is effectively the end of week four, it’s been quite eventful but we’re done with topping and transplanting so we’ll see how she gets on these next few weeks of pure unadulterated vegetation. Vamos!!