Start of week 7 of flowering. Plant looks good, she recovered well and has shown some nice bud development. Temps have dropped to about 32°c.

Bonne journée tout le monde 💚 ❄️❄️❄️❄️❄️❄️❄️❄️❄️❄️❄️❄️ Intensité de la FC3000: 90% Ventilation : Extracteur mars hydro 6 pouces avec filtre à charbon puissance : 4/10 (24h/24h) + 3 ventilateurs à l'intérieur ( ON 8/24h). ils s'activent à un horaire différent. Arrosage : Environ tout les 4-6 jours avec 1.5L d'eau. (+ 1gr de bioenhancer/L eau un arrosage sur 2) Température & humidité : NUIT : 16°C & 70% / JOUR : 23°C & 50% Mon instagram 🌱❤️️ https://www.instagram.com/hou_stone420/

De nuevo familia , reconozco que de las 3 variedades , skunk es mi favorita para destacar entre las demás, NO se espigan nada, los internudos están bastante juntos y la cepa 3/3 ejemplares están muy sanas. Cada una con alguna particularidad genética pero muy sanas. Ph bastante controlado, el trasplante lo asimilaron bien todas, humedad en 55% , siguen sin comida pero por poco tiempo, pronto les daremos algo de caña.

Pow pow and welcome in Week10 - official start of Week4 of flower 🙌 There would be no week without a struggle. After sorting out humidity levels and investing in pro dehumidifier we are now under extremely hot weather hitting 30 degrees Celsius every day. Trying to cool down tent as much as I can throwing ice inside every few hours and hoping that high temps will not effect in any stress that could follow with hermaphroditism - for now all good 😅 Noticed some burned tips on leafs so for next couple days watering with only pH’d water to let those girls use any extra nutrients. Keep it locked and check for daily photo updates 🙌 Day 5 Photo Update. - Those buds are getting bigger every day 😍

🌱UPDATE Day 3 🌱- The plant is alive and has already grown to 3-5 cm! In these early stages, I placed a plastic cup over the plant to boost humidity. It's been hot, and even with a humidifier, I battled very low humidity for two days. Nonetheless, the plant looks healthy and vibrant. 🌱 🌱 UPDATE Day 4. 🌱 - The plant is thirsty - when should I start fertilizing? I last watered it on Day 4 with 200-300 ml, and the soil is dry again today. I'll give it another 200-300 ml, but I'm going to research whether this watering ratio is optimal for my 11 L pot. It's growing well, and the serrated leaves are becoming more robust. 🌿

Strong week. Finished at 2.2 ec. Clean water. Flora flex nutes are top notch. No dirty brown water. Ph fluctuates a bit. If you check on daily you are good.

I let her go another week. The trichome photo was on Wednesday and I was just starting to amber. I’ll harvest her today 22/2/2020 and then do a harvest report in a couple weeks after she’s cured up some.

Day 60 to 67 Little write, they're on autopilot in the autopots

Did some more LST on the bty. Going to let it veg until march before i flip to 12/12, as long as its tall enough. I accidentally topped one of them during lst so it will be an expirement to see how it goes. Light at about 65% inensity until i can keep the tent cool at 100% intensity. Plants looking good, getting a little nutrient deficient so the soil is pretty much done. Will start their feeding regiment with florafex full tilt nutes next week

Busy trimming all week long :) Not much to add.

week 3, the whole family is here 3-gal: Runtz from un-feminized seed - showing female! 3 - gal: Grape Stomper from un-feminized bagseed - showing female! 1 gals: two are black ice gelato from un-feminized bagseed - hoping female! remaining are all fem from SeedSupreme 5 gorilla zkittlez 2 amnesia haze 1 bubblegum last two photos show our 720W Ion LED from Grow Gen that came in yesterday! Note that if you purchase this beasty light you should get S-HOOKS from Amazon or wherever for the tallest hanging option. We are on our way. my 10ml dosing syringes just came in as well as my pH calibration kit. I am running a hybrid philosophy when it comes to Medium and feeding. 70/30 Coco/perlite with the following added in for a "living coco" environment: worm castings, bat guano, kelp meal, alfalfa meal, mycorrizhae, and then I'm giving keyword LOWDOSE GENERAL HYDROPONICS FloraGro,FloraMicro,FloraBloom to supplement any nutrients the amended coco can't or isn't providing. Anyone with experience with this?

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

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