Great harvest! Ended up a little shy of 12 ounces from the two plants, all super dense sticky top buds!! It’s smooth, flavorful and strong! Love it!

Week 8 And now we are here at flower and starting to think this might be a nice smoke she is looking healthy and starting to build your self up. Smells like a mild form of ammi but none the less ammi not long now till harvest now, ill keep you posted

harvesting this tommrow

next time i have to veg more then 4 weeks to increase yield. it finishes quickly and big yield for the short veg time. im also very happy with the optic 4 wich made the buds dense from top to bottom. i might upgrade to cocos or hydro next grow, or maybe both ;)

Buding in progress ✌️

Final day of veg flipping the flower today I’m excited to see how these tests go I have lots of hope for the ones and I may only create s1’s

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Week 11 I though she was gonna be done this week only gave her RO water for the last 2 weeks but looking through the loop her trichomes are still mostly clear so I think I'll give her one more week. She looking great, smelling great, buds look amazing. Tried to get some good video so you can see how lovely she is!!

Black Runtz has some really nice leaves I can tell this plant is gonna strech a lot I hope it wraps nugs around the stem when it does later in its lofe It’s day 41 from seed and all of the plants are really thriving

I started germination of 3 Amnesiac beans on 29/12/2020. I pre moistened my rockwool cubes with ph balanced water to 6.4. Made sure the plugs were just damp and not soaked. Using a small wooden dowel I increased the size of the plugs pre made holes. Than I sowed my beans into the holes. Ripped off a small piece of rockwool and mulched it up. Lightly filled the holes in with the mulched rockwool. Than stuck the plugs into a misted humidity dome, to complete germination. Shouldn't take anymore than 4-5 days to see some sprouts. Once I see some cotlydon leaves bursting to the surface. I will get the plugs planted into some 1 gallon pots. Plus get these ladies situated into their home. Cant wait! Some background information on my first run with Amnesiac. She was super powerful straight out the gate. Hammering off quite the amount of veg growth in 6 weeks. She was a little stingy on nitrogen and really wanted s slightly decreased dose from my norm but nothing to extreme. She was the tallest in the room going into flower and she was quite the stretcher. She gained about 250% size after the first 2 weeks of bloom. Leading to me supercropping her at that point. She didnt mind the hst one bit! Was back to growing and turning her bud sites up withing about 12 hours. She resulted in a great quantity of high quality flowers. Very fat chunky colas just coated in trichomes. Looking to knock her out of the park even further this time around!

19.11 letzter Tag einer guten Blütewoche . VPD 1.1. 2L mit 1.85 Ec. 17.11 Lampe auf 100% PPFD 850-950. DlI 38 - 41. Vpd 1- 1.1. 1.5L mit 1.9 Ec. 14.11 gute erste Blütewoche gehabt. DLI auf 33-36 erhöht. Ernährung bleibt erstmal gleich bis auf ne kleine Steigerung beim CalMag. Ich scorg wieder im Verlauf der Woche.

new home 18/6

I go into the local Hydro store and the previous owner happens to be there. We talk about my novice grow and I mention the "Calcium" issue as I show him a photo.... He immediately asks me " Are those pots on a concrete floor? Because if they are, your roots are getting cold making it look like a calcium issue".... The new owner gives me a nice slab of styrofoam from an old box to put on the floor of my tent as I go home. I sick my BlueLabs PH soil pen in a drain hole at the bottom side of my pot and HOLY CRAP its only 17c and the PH is 5.2 !!!!! I take the plants to the bathtub and flush them with clean water PH'd to 5.8, about 8L of rinse per pot. I check initial run-off and its a whopping 2300ppm/50!!!!! I also remove some of the effected fan leafs in hopes I dont get some sort of plant illness. A day later I do the same PH test, PH is 5.8 and temp is 22C...all is well again. I should have paid attention to growing saying...make sure you get run off checks.

Per adesso non ci sono commenti, sono impressionato e basta! 😝

This week they are all just putting on some weight still a few weeks left to go. Pics were taken on Monday video on Tuesday. The aromas are pretty strong in the tent in general. Shes a struggler but it's too late to turn back now just gunna let it finish.

Another week of Veg gone and trying to understand feeding.... My last grow (the one on the balcony) i overwatered at the start so I made sure this time I would not make the same mistake. I dgave a bit more root booster and found some organic Molasses that i added to the 1st feeding. I know its now considered 'food' but more as an activator of microbes in the earth but I'm sure it can't hurt right? Quite some growth this week, so tried to LST it down a bit... Buuuuut I'm not happy with it, I can forsee spacing problems (should have maybe planted the seeds off-set to one side of the pot to get a better growth going....I'll probably undo the tie-down and just let it grow.