2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

I cannot believe how much weed I have got from this one auto plant also I had to cut four inches off one of the main bud sites because of bud rot I would definitely use Dutch passion again

Day 22 will transplant into 2 gal fabric pots tomorrow Day 24 transplanted into 2 gal pots and fed to runoff. Used grow!t brand coco coir and cheap amazon fabric 2 gal pots. Increased ec to 1.6 Day 26 transplant went without a hitch. Topped her today as she is growing much faster than the other 2. Day 27 fed to runoff

Buds bulking.

New grow , New seeds just been put in water for 24hr then they will be put in paper towel for another 24hr before being planted straight into coco with some mykos an inch or two under. I will update in 48hr. 16/07/18 - all three seeds germed fine all with 1cm+ tails and planted in 80/20 coco/perlite mix with some mykos mixed in and a layer of it 1-2 inch down from where seeds were planted. I watered them each with 600ml roughly before planting and will update when they break through. 18/07/18 - 48hrs since seeds where planted and can see first set of true leaves already, I’ve got a steady 60-70% humidity and staying at 24 degrees Celsius. I’m going to spray them tonight with some ph’ed water with a tiny bit of rhizo.

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And the winner is….. Divine Kush! To say the least, she fought the most and she really shined through. If you see the video above 👆 you can see how wowed we are by her, not only because she sprouted her own seed within herself but also because it shows how much moisture she had to deal with and she sort of found an interesting way to disperse of it! We did try to plant her seedling but unfortunately it did not survive but we are still in awe of it happening before our eyes. Nature is amazing and Our Kushy girl is very much appreciated, she has taught us quite a bit this year. WOW 🤩💚✌️ Plz note, we totally forgot to weigh her.

Fin'ora tutto bene a un mese di fioritura ho cambiato l'acqua, c'è un problema l'acqua è a 25/26° ogni tanto ci metto delle bottiglie congelate e aumento il perossido d'idrogeno

So this week after I gave it under nutrients I apparently gave it too much nitrogen while having a potassium deficiency. Shiney dark leaves, So i fixed that, but some didn't bounce back, and I tried nitrogen. I think they are doing pretty good considering everything I've put them through SO FAR. lol. Nutrients are NPK Raw's total lineup, follow their instructions at first, Fastbuds adjustments as of this week.

Day 50 come along and now I give her full dose off nutrients and she loves it 4ml heaven 4m acti vera 4ml bloom 4ml top max Water her 1 time a day with 2.5L ph 6.4 This time she grow so fast without any trouble she start flowering day 21 and I think she is ready in just 3 weeks Day 54 and she is super happy and easy to grow while my other strain keep mess me around with deficiency lol this strain smell is incredible like light lemon with herb and when you smoke it you feel you smoke hash not week honestly test so close too hash when you smoke it she will be ready in day 70 or 75 and I bet she produce over 150g bud not long left 2 weeks or bit more Day 56 u start use atami bloombastic to increase sugar for final flowering phase

Moved light to 3ft above plants. 400ppfd plants are reacting well. Day 17 moved lights back to 24 inches above and made sure DLI was 45. Huge growth increase.

️️Woohoo! New flavors to taste😍 Post-harvest is truly a wonderful time! ️🎉 4x [Hawaiian Snow x Wedding Cake x Gelato 33] harvested on day 97 / flowering 63 ------------------------------------------------------------ 🔥❄️Drying : In my 1M² box 8 Days in the dark Temperature : 14-18°C Humidity : 50-75% Extractor+ carbon filter ON 24/24h ------------------------------------------------------------ Weight of buds after manicure and drying( in grams): Plant A : 24.4 grams Plant B : 17 grams Plant C : 20.2 grams Plant D : 16 grams Total : 77,6 grams ------------------------------------------------------------ 🎪Harvest report in my 1M² box with 16 plants (5 strains): Total 291 grams of dry, manicured buds. I set myself a goal of at least 300 grams. So I'm not entirely satisfied on this point. I think in the next grow I will use other techniques, like LST, topping and larger pots to try to achieve my goal!✌️☮️ ------------------------------------------------------------ 📜Links : 🌱 Seeds from the USA x Landrace collection of GreenHouseSeedco : https://shop.greenhouseseeds.nl/feminised-cannabis-seeds/usa-and-landrace-limited-edition/ Food for your plants 🔥🔥👍 https://www.greenhousefeeding.com/ 👨‍🚀My Instagram 🌱❤️️: https://www.instagram.com/hou_stone420/ ------------------------------------------------------------ ☮️Thanks for your visit💚☮️

Good morning growmies! I am finally back online after moving house and being without internet for weeks. These ladies took a battering and bruising in transit, and were left in the dark for 4 days, starved of water and food for 9 days TWICE inside 3 weeks. Still, can you believe the progress despite all that? Wowza, when RQS say these Epsilon F1s are fast they are NOT JOKING, and OH MY the smell is soooooooooooooooooo good. It's really a complex sweet and fruity scent with delicate notes of spice and sandalwood. I am super curious now. All five plants are within a couple of inches of each other, the tallest being 72cm currently (Dinah!). I am so very impressed with these plants that I am considering running a full tent of Epsilon F1s for my next grow! I wonder if I can get a bulk discount?! 😬😁 Day 54: Fertigated 10l Day 56: They are not slowing down. Ally, Normani, Lauren, and Dinah are all similar size, Camila is a bit smaller but in terms of development they are all very very close to each other. I am really impressed by the uniformity here, more impressed by the speed, and most impressed by the scent. They are not strong smelling, but if you get close or have a squeeze it wafts over you like an exquisite perfume. It is the most complex cannabis scent I have experienced yet. Really really interesting, I am really excited for the eventual smoke review so that I can properly understand the complexity at play here. The breeder and sponsor of this grow Royal Queen Seeds have offered to sponsor a full tent of this strain, if that works out then I will be doing another grow series right after this one. Fingers crossed!

Did 10l two days ago and it’s bone dry, from 5 to 10l every two days or less , Just a rain water this whole week . She stacking nice , heat still burning some terps but it’s not as bad as 28C+ Clones recovered too and doing somewhat ok , glad they had little stunt so I have time to finish mom off and then repot them

Pretty easily sailing tbh

As I said, not happy with the Harvest, I have uploaded a video which I explain more. But I will definitely run her again and do a better job when growing and paying more attention 👍🏾

Had to remove the net to gain better access to the plants.

82.5 grams she taste so good ... thanks for all the love everyone