Ladies looking really good 😎👌🏻💚🙏🏻 they are in two different tents but all from the same beans rdy to chop of at any time ! dense flowers strong smell !

Today was the day, unfortunately about a week in advance because of an infestation of Mites that reached the buds of all 4 plants left. After reasoning, interrogating AI and watching a video, I had to harvest in advance and I decided to do a washing with H2O2 as per this video explanation of Jorge Cervantes, available at this link (https://youtu.be/S7jE7qzfgQs?si=eC6klOXkWz9eBJO3). I am providing photos and video also of this step I made.

9/7 She was showing me about 10% amber, so i decided it was time to hang her. I dont have hanging scale yet, might get one today actually. She was quite easy to grow. Ill keep updates here on the drying process. 14/7 she is drying nicely, still in the wet side after 6 days of drying. Nice and slowly does it. I hate the waiting game in the drying process. I want to light her up and see whats shes made of. :)

Looking nice.

Handled the transplant really well , she’s starting to branch out nicely. I’ll start some LST in the next week or so

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

She’s doing the thing! No stress from the trellis and is stretching lots! She already has recovered from cal mag deficiency as she has no signs of it or any deficiency in new growth. New gravity fed watering system installed today, super excited!

Training Day 💥 It’s been an exciting and productive week for my Auto Pablo Escobar from the Divine Seeds Competition V2. After topping her recently, I introduced Low Stress Training (LST) to shape her early structure—and she’s responding like a natural. 💪🌿 🔸 Topping was done earlier this week – recovery was smooth 🔸 First round of LST applied gently to open up the canopy 🔸 She adjusted beautifully, showing no signs of stress 🔸 Growth has become noticeably bushier and more controlled 🔸 Side branching is coming in strong and symmetrical 🔸 She’s forming into a sturdy, compact plant with real promise Nutrient & Care Regimen: 🔹 BioBizz Grow – 2 ml/L for healthy vegetative growth 🔹 CalMag – 0.5 ml/L to support robust structure 🔹 Homebrewed compost tea with Effective Microorganisms to boost microbial life and root health 🔹 pH adjusted to 6.4, measured and stable 🔹 EC held steady at 900 µS 🔹 Dechlorinated tap water used for all feedings She’s thriving under consistent conditions, and I’m very pleased with her current pace and shape. Her posture is low and wide, just how I like it at this stage—easy to manage and full of future potential. 🌱🔥 Looking ahead, I’ll continue shaping her gently as she moves closer to preflower. If her early vigor is any indication, we’re in for a flavorful, frosty finish. Let’s keep the momentum going! ✌️🍀

Man this thing is going from strength to strength. Just stacking hard. Smells amazing, looks amazing, grows amazing. Hard to fault this grow, the training technique this round worked awesome. So much frost and the deep red 😍😍😍 Pretty chuffed with this one. Did some defol as she only has a few days left. Nugs are rock hard so can’t wait to see the weight.. plain water and shooting powder to help stack

She's coming on beautifully switching to flower sometime this week so it doesn't get to crammed in there hopefully. did the final bits of tieing added the second hps last week and will begin with buddy and terpinator tomorrow thanks for reading happy growing guys

Iceoletor blueberry ✅🥇🏆

Su crecimiento desde primer momento fue genial y para ser más sativa es muy temprana, muy contento

RipperSeed´sLimited2022 Sour Face x Animal Cookies 12-12 day35 Iguana Juice bloom Advanced Nutrients 3ml/1l (water-osmosis0.5l+tap0.5l-ec0.3)

2/26: Harvested both Black Jacks and both Gorilla Girls tonight. They got a rough live trim, then chopped, washed, and hung to drip dry. Big, heavy, frosty buds on all 4 of them!👍 3/2: Still drying in the closet...amazing aromas!

Kicked the Critical Kush out to let the Lemon AK have the tent to itself to finish. Tall, strong, some yellow leaves think it might be a ph issue, flushing a bit and continuing to trim and monitor, it's not widespread and the plant is otherwise healthy. Did a little trim and LST on day 48 to help it take up the extra space that is suddenly available.

Schönen Sonntag und 3. Advent euch =) ! 🌱 Wochenupdate Die Woche lief mal ohne Drama, zum Glück =D Ich habe kurz nach dem Stretch alle Maßnahmen eingeleitet, um das Canopy sauber zu formen. Die ersten sanften Klima-Anpassungen sind erfolgt, und wir bewegen uns auf die Blüte zu – aaaaw yeeeah =D Wie ich ja bereits erwähnt hatte, wollte ich nach dem Sonden-Fix erst einmal visuell beurteilen, wann der Stretch wirklich vorbei ist und wir final auf die Meshes sortieren 😊. Die Sonde läuft inzwischen wieder zuverlässig, aber zu diesem Zeitpunkt konnte ich mich darauf leider noch nicht voll verlassen … Ich habe also noch einen weiteren Tag trainiert und bei jeder Kontrolle darauf geachtet, wie weit sie über das Netz drüber sind und ob es sich dabei um echten Stretch oder nur um Rebound vom Runtertrainieren handelt =) Dienstag hatte ich dann deutliche Anzeichen, dass wir endlich durch sind 😄 Ab hier habe ich jeden potenziellen Trieb auf ein Mesh verteilt und die, die etwas zu weit waren oder noch gebogen ins Netz zurückgeholt 😁. Als das Canopy „locked“ war, habe ich ihnen erst mal ein wenig Ruhe gegönnt. Da ich nach 12 h kein Zeichen von Stagnation durch Stretch-Erholung und Umverteilung feststellen konnte, konnte ich es nicht lassen und habe das komplette Canopy defoliert =D Der Gedanke dahinter war einfach, so früh wie möglich viel Licht an die jungen Budsides zu bekommen 😄 Jedes Blatt, das Schatten geworfen hat oder keinen direkten Nutzen hatte, wurde entfernt =) Sie haben es mir direkt bei der nächsten Kontrolle gedankt 😁 Hier habe ich dann das erste Mal echt mit mir gehadert. Die Frage war, ob ich jetzt nach Lehrbuch fahre und ihnen ein paar Tage Ruhe gebe oder ob ich direkt weiter im Programm mache und die Under-Canopy-Defo hinterherschiebe. Es wäre schon ein Traum, direkt zu Beginn vom Aufbau einen guten Energiefluss zu haben =D Die Ladys standen absolut vital und haben förmlich dazu eingeladen … also habe ich natürlich direkt durchgezogen und direkt unter dem Netz defoliert =D Hier wurden alle Spaghetti-Triebe und alles, was nicht oben im Netz angekommen war oder kein echtes Potenzial hatte, entfernt. Alles, was geht, soll ohne Umwege in die Tops gehen. Und bitte kein Popcorn =D =D Anschließend habe ich noch die Luftzirkulation angepasst, damit unter dem Netz wieder ein schöner Luftstrom entsteht =) Man sieht sogar wieder, welche Pflanze welche ist =D ! Jetzt geht’s auf in die Blüte – bin gespannt, wie es weitergeht 😊 🌱 Pflanzenstatus & Training Alle machen sich gut =) Bei Notorious habe ich das Gefühl, sie stretcht deutlich buschiger als die anderen – sehr viele brauchbare Triebe, wirklich eine tolle Scrog-Plant 😁. Bei Purpz ist mein Eindruck, dass sie deutlich mehr versucht hat, nach oben zu kommen. Hier muss man wirklich hinterher sein, wenn man sie sauber scroggen möchte. Blueberry Cupcake – absoluter Performer 😄, damit habe ich aber auch schon gerechnet bei dem, was ich über die Genetik gelesen und gehört habe =) Zu guter Letzt das Sorgenkind Guzzlerz: Hier wird auf jeden Fall noch mal was kommen in Zukunft. Leider hatte ich massive Probleme mit der Keimrate. Die einzige, die aufgegangen ist, ist schon mit einem Keimblatt zu wenig ins Rennen gestartet und konnte einfach nicht so gut hinterherkommen. Trotzdem füllt sie eine kleine Ecke im Netz und sieht auch sonst im Vergleich top aus 😄 – nur eben kleiner. Da werde ich auf jeden Fall noch mal testen, wie ein guter Seed performt 😊 ⚙️ System Nach wie vor selbes Vorgehen mit Top-Ups =) Aktuell brauchen die Ladys deutlich mehr Wasser als Nährstoffe, weil nach der Defo und dem Canopy-Sorting der Wasserverbrauch durch die Auxin-Umverteilung und die erhöhte Transpiration deutlich ansteigt =) 1,1 kPa VPD 700–800 ppm CO₂ im Durchschnitt EC der Lösung wurde auf 1,9 EC erhöht 1000 PPFD Vielen Dank mal wieder fürs Lesen und die Anteilnahme, nur liebe für euch =) Eine schöne Woche =)

Larry und ak wachsen gut, die Autos unterscheiden sich etwas in der Wuchsgeschwindigkeit, obwohl gleicher Boden, Wasser zu und genetik 🤷

Easy strain to grow, tolerant a high ppm. Very good smoke. Buds are not very dense.