Week5 March 1st day 30 I transplanted one( the one with no broken branch) inti the 7gal today watered and did some LST I really hope it adapts to its new home I’ve never transplanted before I used extreme garden MYCOS hope it works also moved the light up a bit and plan on going 100% once I transplant the other one March 2nd transplanted the other one (broken branch one) my shop didn’t have 3.0😔😔😔😔😔😔😔 so i used DNC Great Lakes water only soil hopefully this works and I mixed some 3.0 I had left from the other pot cross fingers for nice transition to its new home March 5 been three days since transplant can’t really tell if they adapted to there new homes yet some sings of stretching since transplant I’ve never transplanted before and I’m not sure if they need water or not so imma chill let them go for couple more days then I’ll water scared to over water them right now since I transplanted them don’t want to over stress them other one in 3.0 seems little droopy to me compared to the one in Great Lakes but Great Lakes soil seems bit dry sooooo idk I’m going keep an eye on them

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Coming throw - we put a few in a 20liter - going for a few tiers. Tent has opened up some space from harvesting this week so should see the remainders beef up some more. Weird that I have flies in this tent. Harmless but I think the sweet aroma maybe have something g to do with it - or coffee grinds -

starting to grow normally after recovery. Will be doing more LST and FIM'ing the near future. Hopefully wont need much more veg time. Going to try and stimulate growth this week with some natural products

Half time in bloom. I made a video with and one without flash. This is always a bit misleading. I set Josefine a bit higher. I should have done this much earlier as she was already the smallest the whole time. I defoliated a tiny bit. Should be. A few leaves are turning yellow, but I think I watered a little too much, or gave too much fertilizer. However, it doesn't look alarming to me at the moment. They'll catch themselves again. Otherwise, I'm looking forward to the first week of April.

Ok so after some much needed time to think about this grow I've gone and git a new tent 4x4x7 and will be putting all of these ladies into flower together .. The ladies are all doing good and are healthy happy ladies .. They have been stuck outside with supplimental lighting in am .. This weekend will be transplanting and treatment to get them ready to be brought inside for good I haven't seen any bugs yet but we gonna clean these ladies.. tent gets cleaned every other day ... The amount of excitement I have for there beatys I'd unreal ...cheers and thanks for stopping in..

22/5 just given them 20l fresh water per tray which is done (2 times a week). Feed schedule 📅 changed 2.4ml per ltr (bloom) 1.8ml per ltr (micro) 0.8ml per ltr (grow) 10ml per 20 ltr tray. (Flowerbomb) Had a problem with (smaller plants) tray. Which was too with pump luckly i have a spare so have changed them over. I have kept this tray on a pre Flower schedule now they have a better pump i believe they will pick up speed. So far so good fingers crossed 🤞 😊

Day 44-21/05/22 everything is looking good I might take them out tomorrow do some light defoliation and move them about abit not sure yet!!!! . Day 47-24/05/22 they look real good today!!!!

Very sticky the gorilla glue it ended up with oil leaking from the buds i believe that was due to genetics and a new terpene booster im using which helps with the plant producing more oils and terpenes, rosin all though it took almost ten weeks for this plant to flower it turned out great i will be growing this again as i have seeds on the way already

Moved to feeding .5 liters every other day wondering if they are still be watered too much. Any advice would be appreciated as this is the first time we have grown.

02.10.2022 - 56 days since my little girl sprouted from seeds🌱 Fourth week of flowering of my BCN Power Plant 🌴🌴🌻 Nutrients: Jungle Indabox - I serve the same as last week Advanced Nutrientiens - Bud Candy - carbohydrates for my plants 🍭🍭 Building Stimulators 🌼🌼🌼: Jungle Indabox Environ X Advanced Nutrientiens - Big Bud - I applied this week for the last time ATAMI - ATA CALMAG - When growing under LED lights it is recommended to use Calmag and Atami is an excellent brand The plants tolerate the nutrients well, some of the lower leaves showed slight signs of burning, they turned a little yellow and the tips of the leaves were brown 🍃🍃 Therefore, I will continue to use only one Bud Stimulator and that - Jungle Indabox Environ X, I will leave Big Bud from Advanced Nutrientiens for other plants. Training: Defoliation: I still remove the large and lower leaves so that they do not shade themselves 🌞🌞 I'm not planning a bigger defoliation until next week, the girls are a little leafy, but not enough to need more trimming 🌴🌴 General: The girl is already blooming strongly, but she is the slowest in the box so far, I would say so in a week 🌸🌸, the branches reach the same height, exactly as I wanted 👍 At the moment she has more leaves than I would like, but I will do more defoliation later, as I wrote 🍃 I will adjust the nutrients from the next watering, I will leave the only bud stimulator 🏵️ 🏵️ The girl no longer grows tall, she puts all her strength and energy into her buds 🌸 Light: Believe - the best light of sponsor Mars Hydro TS 1000 💡💡 Smell: The whole box already smells a lot, the smells mix and flow out of the box, when I open the tent, I get a strong smell of flowering cannabis 🌶️🌍 Thanks for your opinions, smile and fun with my diary, you can follow me on Twitter 🐦: @targona666 See you soon 😍

Day 48 of flower just cruising along.

🔘Week 4 Over for Lady North 🔘 Her growth was steady and constant so I went in for a full defoliation LST 🔘The goal is to level up the canopy to the same level so the tops all get the same light priority 🔘 I introduced nutes to the feed schedule around week 3’s end going into week 4 🔘Let’s see how she handle this

Welcome to week 12 💚 I hope for good flower production on the PR and SD this week. Still not sure if the Sundae Driver is a hermie or not, maybe it recovered but i have no clue. The Strawberry Gorilla should continue to put on some weight, I hope she fattens up this week. Happy growing to everyone!

We’re officially in the first week of flowering, and the stretch has already started! 🌿 The plant without LST has shot up quickly, growing tall and strong. However, I noticed a few rusty spots on the leaves, which might be a calcium or magnesium deficiency. To help, I’ve slightly increased the CalMag and Bloom nutrients for that plant. 🌱 Other than that, everything seems to be going well, and I’m happy with the overall progress. It’s exciting to see them enter this new phase, and I can’t wait to see how the buds begin to form! 😁✨