First feeding of the week was a light 300ppm and the rest RO. Death week two is going well. She is eating up what is left over in the leaves giving me confidence of a good flush. Last grow she was bright green on harvest and incredibly hard to smoke without a long cure. I can’t be fooled by amber trichomes, because of last weeks heat problem. The leaves are full of amber but I’m still seeing some clear. Last week countdown right on cue. Lights reduced to 90% on the last day.

Flowering day 25 since time change to 12/12 h. Hey guys :-) . The ladies are developing more and more beautiful and fat week after week 😊. The tent fills up perfectly 👌. I removed the bottom shoots to allow the energy to flow to the top buds. This week it was watered 3 times with 1.3 l each (nutrients see table above) Otherwise everything was cleaned and checked. Have fun and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : Clearwater Seeds Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany

Estuvo bastante bien, esto lo lleno con lo que recuerdo para que no borren este diario que es una suma de mis cultivos pasados. Saludos!

Semana bem excitante uma vez que as flores começaram a aparecer. Continuei espalhando os galhos na grade para que cada flor receba luz. acabei tendo que tirar algumas fotos noturnas pois não estava chegando em casa a tempo de vê-las durante o ciclo de luz. Ainda regando apenas com água.

The week is progressing wonderfully, it's been 8 or 9 days since I flipped them and they have completed the transition and are really starting to stretch. CF#1 is 24 inches, CF#2 is 32 inches CF#3 is 25 inches and CF#4 is 27 inches. #2 by far has been the biggest and quickest developer of the 4. I wonder if the fact that it is directly under the CO2 bag?? I fed them 1.5 tsp of the g.h. flora series this week, called myself stepping it up a notch. The second feeding I fed them plain tap water p.h.'d to 6.6 ish. I upped the amount of water to 3 liters for one feeding (the one with the nutes) it created alot of run off, which the plant eventually sucked up. I was a bit surprised because I thought for sure a 10 gallon pot would take atleast 2 gallons of water or more to see runoff. In this case it appears a little over 2 liters until the first signs of runoff. This is the first grow where my plants have made it this far without showing nute burns at the tips, they seem to be loving my feeding cycle. I plan on adding another trellis net in about a week or two, once they stop stretching, I know from experience with this strain the buds are going to grow nice and dense and cause the branch to sag over without the extra support.

Day 79 - The start of week 12 and she’s coming towards the end of her life. Her leaves are all starting to fade and she’s almost completely milky but I want more amber so I’m thinking another 1-2 weeks and she’ll be ready. Until then she’ll get plain water and one more watering with Calimag and Sugardaddy to try and give her one last boost. Day 80 - Well this girl will probably get chopped this weekend. She’ll get one more drink of water with MagiCali and Sugardaddy tomorrow and she’ll dry out till chop. I wish I could get good trich pictures but just won’t work but she’s about 10% amber right now so at the rate she’s going, chop day will be sometime this weekend. Day 81 - She’s really starting to fade out now and getting more and more amber in her. I really wish the buds would fatten up a bit more but the ones that are there and there are plenty, are pretty damn dense so not complaining. I gave her one last watering with calimagic and Sugardaddy and might end up giving her one last plain watering before harvesting but either way she should be ready by the weekend. Day 82 - Nothing much to speak of today, she’s gonna get the chop this weekend, she’s got more and more ambers popping up so just gonna let her dry up now and most likely chop on Saturday. Day 84 - The end of week 12 and haven’t decided if I’m going to chop her today or tomorrow. I’ll decide a little later today but just got some last updated pictures and will update again once I chop.

25/8 Start of week 4 from seed I believe, plant 1, being fed 'Easy Boost Organic Combo' has the dry mix stuff in the Coco, added the 'part 2' tablet ended up mixing with 4L of water, says it lasts a week mixed up so I gave her about 0.5L today as she was really dried out. Plant 2, being fed 'Canna' colour seems to be getting better, I'm going to continue giving her canna CalMag & Rhizotonic' until she looks like she wants a bit more. All going well though, both plants are still alive after their topping! 🤞🌱💚 Man I need a bong now 🔥💚 26/8 both plants recovering well after being topped. Not been watered or fed since yesterday, will wait until they are drier. Plant 1 is going to be a lot more short and bushy by the looks of it (which is what I need in grow space). 27/8 plant 1 recovered a little faster, shed almost picture perfect, happy 1 plant 1 though. No feed or water again today 28/8 think I seriously overwatered plant 1 😂🙈 live and learn. Going to let her rest for a couple more days. plant 2 doing really well, unwilling start LST soon and possibly top a couple of sites early next week 29/8 plant 2 is taking off again, I've started LST on both of them. #1 is not as developed, I removed a few leaves off her today as they were preventing proper airflow. Plant 2 has been fed for the first time in a while, plant 1 has had a tiny bit of water 30/8 had every intention of transplanting both girls, however I only had enough coco for 1. Decided to transplant #2 in to 7Gal fabric pots as she is the most developed. Both have roots starting to push through the bottom of the old pots, may have left it a couple of days too long. 31/8 Coco coir never arrived for plant 1 so I'm hoping it come tomorrow. Plant 2 has been topped quite a few times today letting her rest now. Not sure what plant 1 is doing it could me getting root bound? Gave plant 2 some calmag water, nothing for plant 1

08/29/2018 Been a hot week but the girls don't seem to mind,90s in the day 82° at night and sticky,muggy weather. Their leaves all pointing ☝️,lets me know they are happy ☺️ .watered with plain water they were pretty dry pots weight was very light. Will feed and water in in morning sleep well and medicated😎 08/31/2018 Just a note today #1 is very green compared to the other 4 plants, could be potted plants running low on nitrogen? My soil has plenty according to soil test. I know last year's crop in soil was pale like other 4 plants, maybe biochar is helping soil retain it's nutes better? Year 2 for biochar, first year could have tied up some nutes....hmmm? Ps Last feeding today Fox farms big bloom at heavy feeder dose 1/2 cup per gallon, should see nice gains this week! 🙏🙏🙏. Pss. G GTH #4 loosing her smells, #3 smells of Skunk and coffee ☕,#2 minty chlorophyll orange,#1 menthal,skunky but very lite scent. Should I pollinate a branch of each? Oh my God the pressure! Do I have enough pollen? Oh sorry for rambling on just smoked lemon sour diesel and thoughts very racy😀 Got to garden see you next week 😎😎😎😎😎😎😎😎😎😎😎😎😎

Отличный рост , ну а дальше все сами увидите на фото и видео . Радует душу , глаз и вскоре будет радовать тело :)

Jun 10: Watered less than half gallon of Ph 6.7 water. Jun 12: That watering really gave a growth spurt! Bumping the humidity up to a max of 81% til early flower. Jun 13: Plant is tall enough to tie down the main stem to the side. Once the other stems get longer, I'll tie them down to the rim of the bucket as well, to maximize light exposure. Jun 15: Buckets are getting lighter, but the soil is moist, meaning my humidity was too high. That lead to some mold and algae that I will treat with spray Neem oil. Smells terrible, but should do the trick. Lowering humidity to 65% for a bit, then will probably get closer to 75% over the next few days. Decided to top, since the plant is getting big enough to support it!

Hey everyone its the final week for this beauty.what a pleasure to grow she was and i will grow this strain again for sure. She drinks a whole lot less then before and the trychomes are turning amber and the rest are milky so she will be cut some where on the end of this week. I will update you guys and girls with the final dry weight and some pics of the dry buds. Thank you rqs for this amazing strain. See you all soon✌️

Día 69 (01/07) Riego con 1 Litro sólo H20 - ph 6.2 Día 70 (02/07) ¡ Cosecha Banana Purple Punch Auto ! 😁 💦Nutrients by Aptus Holland - www.aptus-holland.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae

Floracion (Tiempo estimado 64 dias) Segunda Semana de Floracion 15/01/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 1000-1250, Ec 2.0-2.5, Temperatura 20°C - 26°C, Humedad 75% Ambiente: Temperatura 24 °C, Humedad 65%, Ventilación 15%, 12 hrs de luz , 12 hrs de obscuridad. 16/01/2024 - 5:00hrs y 22hrs: Se hace cambio de solucion nutritiva, con los mismos parametros de la semana, Se lavan recipientes, Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 1000-1250, Ec 2.0-2.5, Temperatura 20°C - 26°C, Humedad 75% Ambiente: Temperatura 24 °C, Humedad 65%, Ventilación 15%, 12 hrs de luz , 12 hrs de obscuridad. 17/01/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 1000-1250, Ec 2.0-2.5, Temperatura 20°C - 26°C, Humedad 75% Ambiente: Temperatura 24 °C, Humedad 65%, Ventilación 15%, 12 hrs de luz , 12 hrs de obscuridad. 18/01/2024 - 5:00hrs y 22hrs: Se coloca una red extra para separar los tallos secundarios y entre mas la luz entre las ojas, Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 1000-1250, Ec 2.0-2.5, Temperatura 20°C - 26°C, Humedad 75% Ambiente: Temperatura 24 °C, Humedad 65%, Ventilación 15%, 12 hrs de luz , 12 hrs de obscuridad. 19/01/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, al acomodar bien los tallos secundarios por la red por accidente troce una rama, coloque savila para ver si reconstruia la pared celular rota, queda en observacion, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 1000-1250, Ec 2.0-2.5, Temperatura 20°C - 26°C, Humedad 75% Ambiente: Temperatura 24 °C, Humedad 65%, Ventilación 15%, 12 hrs de luz , 12 hrs de obscuridad. 20/01/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, no se observa mejoria asi que se procede a cortar ese tallo segundario, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 1000-1250, Ec 2.0-2.5, Temperatura 20°C - 26°C, Humedad 75% Ambiente: Temperatura 24 °C, Humedad 65%, Ventilación 15%, 12 hrs de luz , 12 hrs de obscuridad. 21/01/2024 - 5:00hrs y 22hrs: Se verifican parámetros básicos del agua, mantener cuidados específicos. Agua de osmosis: Ph 6.0, PPm 1000-1250, Ec 2.0-2.5, Temperatura 20°C - 26°C, Humedad 75% Ambiente: Temperatura 24 °C, Humedad 65%, Ventilación 15%, 12 hrs de luz , 12 hrs de obscuridad.

Day 26 – On the Verge of Flowering We’re now on Day 26 of the grow, and the Sweet Skunk Automatic is entering a critical stage. After nearly four full weeks of vegetative growth, the plant continues to appear healthy and structurally compact. Leaf growth remains vigorous, and the internodal spacing is still tight—a good indicator of strong light exposure and stable environmental conditions. This week marks the likely transition period into the early flowering stage, which typically begins around week 4 to 5 for this autoflowering strain. While no pistils are visible yet, we expect the first signs of pre-flowering to appear within the next few days. Weather Recap & Outlook The past week brought mixed conditions—cool nights and scattered showers slowed growth slightly but didn’t cause any stress symptoms. Now, a more favorable pattern is setting in: • Daytime highs are climbing steadily toward 24–26°C • Nighttime lows remain above 10°C, reducing root zone stress • Plenty of sunshine is forecasted for the coming days, especially mid to late week These improved conditions should provide the perfect push for the plant to fully enter bloom. We’ll be watching closely for the first white hairs and signs of stretching. The stage is set. If everything stays on track, we’ll enter the flowering chapter of this grow very soon

unstoppable :p Day 29: on the video, the 2 on the right side are the Ayahuasca Purps, the other ones are Peyote critical. getting real bushy, need to defoliate soon day 32: they got their first feeding, I only use a minimal amount of blooming nutrients. day 33: plucked some leaves , cuz there was no airflow at all, also installed a new ventilator. day 36: had to pluck some leaves again

Hbss is 27in tall ABxRKO is 38.5in tall I'm starting to run out of height because of the ABxRKO as it finally flowers. Still slight nute issues with the HBSS, but nothing too tough to handle for now. The smells the HBSS give off are absolutely crazy. Just letting them grow, and watering every day seems to work. Will upgrade to earth boxes for this tent since a little autonomy is nice

The seeds are really starting to swell up nice. These ladies are bearing alot of seed from the natural style of pollination i used. Decided to use a small paint brush and some of my collected pollen. Dusted the un seeded flowers and got these ladies seeded to the max. In a couple days I will give them a good water shower to wash away some of the excess pollen spillage. I'm going to let these beans get nice and mature. Viability won't be a question lol. After I collect/harvest my seeds I'm pondering what to do with the plant material. They are throwing off a solid amount of resin to protect their offspring. There will be plenty of it to work with. Decisions ...Decisions . Until next update. Happy growing and stay lit fam.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hi all buddies! First crop from hydro nft growth. Growth went well, I had some management problems in changing nutrients from veg to bloom which delayed the harvest for a week or two. Sensi Seeds' Skunk # 1F is incredibly resistant to mold and insect attacks, very close internodes ensure low height and compact buds. Unfortunately I chose the mother phenotype wrong, I found the lowest as I wanted but I lost the fatness of the buds present in other phenotypes, but the game was worth the same and I am very proud of it! I collected after 94 days of flowering, the last 72h 20kg ice cubes in the tank and total darkness which the last 24h without irrigation. After cutting I hung the whole plants in the tent for 24 / 48h 19 ° C 55% HR. Two days of wet trim to 8 girls and I hung their colas in the tent to continue drying. With a girl i tried dry trim but i will avoid next time. After a week in the tent I cleaned the colas and removed all the sticks, I placed everything in airtight bags in the refrigerator for 5 days 12 ° C 65% HR (daily check). Now dark storage room at a temperature of 18 ° C 65% HR. At the last weighing a few days ago it was more than 500g, from now it does not lose any more weight. Next week I will take pictures of the weigher and the finished harvest🍀🍀🍀 target!!🍀500g dry/cured🔥 Thank you GD community you are precious for everything, criticism, advice, curiosity, thanks 💚🌱✊🏼 Awesome people here on GD🔥🔥🔥