OK guys I'm starting at the beginning of week 5 so this one is only a few days after my last addition the the diary. I noticed the very first pistils so now we are in week 1 of flower. Punch 2 has dark greeb, curled down edges, maybe a nitrogen toxicity? I don't think I'm over watering, they didn't have water for 6 days when I noticed these symptoms. Punch 1 doesn't have the dark green edged fan leaves or the curling down, but there are some pale white spots and smudges at the ends of the fan leaves. Any ideas guys? I'm new to this so not 100% what is going on or how to fix. Any suggestions would be greatly appreciated. Thanks guys🎄

She has been a beast and very easy so far. She does like it warm at least 80 and she happy. Using her as a mother plant to gets some clones.

She is loving life for now! No problems at all in these 10 days, she grows good and seems to like the light a lot, more than my previous plant. In the first 4 days in stretched a little too much but I buried part of the stem and lowered the light. DLI at the moment is 24. I gave her this morning her first micro feeding. 800ml of phd water with 1/5 the dose of BioGrow and CalMag. All is well and I'm very happy of how everything is proceeding. Thanks for stopping by, till next week!

End of week 11 (day 77) in bloom and the end is near. I already cut one plant and i will cut another in few days. The 2 Panama plants are starting to getting red! I monitor the trichomes with microscope and i am waiting patiently! Buds are getting thicker and trichomes milky!!

Sorry for the lack of updating recently I have a new job & don’t have much time for anything but I just wanted to show how amazing these ladies are doing! Both Strains smell unbelievable & by far some of the frostiest plants I have grown, have taken to fox farm nutrients very & they have not really gave me any issues the entire grow! Will post some more updates around harvest time thanks for following friends!🙏🏼🙏🏼 Happy growing🌱🌱

Creciendo muy fuerte 💪

I trimmed off a bunch of fan leaves, but still not as many as some of the more experienced growers. I started dropping down the Flora Grow and Micro. I hope they finish strong. Pictures are all from the end of week 5 of flower.

Sono felicissimo,abituato alle hps e cmh e non riuscivo ad ottenere grandi risultati, con una hps 600 watt massimo 450 grammi,con un led 730 ho fatto 1500 grammi,waooo😍😍😍

Had a good week with these babies, except when I found my cat laying on the biggest of the three sprouts. I sprayed the ish outta her with my spray bottle, she’ll never venture into my garden again lol. Luckily the plant survived, I trimmed to leaves that were crushed. Hopefully it doesn’t get too stressed.

First auto run! Fastbuds , Seedsman & Victory seeds! Never done autos before so excited to see how this goes! Day 5 and 2 of the NHL’s from Victory seeds still hasn’t popped😫😬

Have lights on sunrise sunset. The been testing out best high intensity lighting schedule. Wish I had c02 to add into the tent. Plants were getting 1600-1800 par for 3 hours a day. I noticed they were getting slight tip burn so I lessened the light intensity a little bit and extended the peak sunlight from 4 to 6 hours. Now they get 1300-1400 par for 6 hours a day. They are getting really good air flow, humidity, temperature. I've been replacing the water every 2 days now and topping off almost every day. Just switched the ph from 5.8 to 6.5. I think that's recommended for flowering. Currently they get 2200EC I use ro water. They get 250ec of cal/mag. 400ec of lotus boost and 1400-1600ec lotus Bloom

Already flowering, can’t wait 🎆🔮💜

Transplanted into larger containers with fresh soil. The soil is a blend of Peat Moss one Perlite. I then mixed some older soil with the new Peat/Perlite blend. After the transplant I watered with Recharge to help get some healthy microbes starting to develop in the soil. In addition to transplanting the 2 existing Blackberry's I also planted a fresh seed. It should be interesting to compare the new seeds growth started in fresh soil compared with the girls who struggled in the old soil. I'm hoping to see some nice improvements over the next few weeks. Stay tuned...

WEEK IN 🌸 TOOK OUT MOST INTERNODES GROWTH IN VEG & LEFT ONLY TOP GROWTH!! HOPEFUL MY PLANS WORKS OUT WITH THESE RUNTZ DURING THE STRECH‼️🙏🏿#runtzf1durbgushers 🎩🌈🍬

it is stable in the wind, withstands hot days and continues to grow. it is showing the first signs of flowering. i think it will go into full bloom next week. she still has 2 months before i move. i hope she makes it. she gets fertiliser every 2 days.

Bio PK 2-5-8/2ml E2W —————— 4.9.2025-11w grow/5w bloom

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Pretty girl ❤️