Hello. This is week 1 of flowering for The Dos Si Dos 33 for Me by Barney's Farm. I put old fertilizer on about 3 weeks ago and the plant have mostly recovered from that. According to the internet I'm getting the Spinosad I ordered today. I also ordered some nematodes a few days ago. I'll have to read if Spinosad can hurt the nematodes. I think I might use the nematodes in my garden first. Most everything else is going good in the grow room. Plant #3 still has some curly leaves but it is getting better. I did the rub test on the plants and plants #4,#2 and #1 had the most smell and the stickiest resin. Plants #3 and #5 had the least smell and resin. Plant #4 is leading for the best smell and stickiest resin and is fairly compact. I'm trying to start some OG Kush seeds from Sensible Seeds Premium Selection for my next diary. I emailed them to find out who made them but I got no response. These seeds were free with my order and I've had them for about a year now. Hope they start. Till then. Chuck.

Overall diesel smell great frost beautiful waiting on one more of this strain looks like some indoor gas super excited some of the best outdoors I’ve had yet

Another week.struggling with mold because of high humidity..I will give 5more days and then 48ho darkness…almost there 🙏..

Buds are becoming big. Decreased nutrients. Will flush next week. Can't wait to dry and taste...

Hi everyone 🤗. They continued to develop well this week 👍. Slowly they stop growing and put their energy into flower production. So that this energy is not wasted, the lowest shoots on which only popcorn buds grow anyway have been removed. Next week the new update will follow as usual, stay healthy 🙏🏻 and let it grow 🌱 You can buy this Strain at : www.Zamnesia.com Type: Runtz ☝️🏼 Genetics: Zkittlez x Gelato 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205 W 💡💡☝️🏼 Soil : Canna Bio ☝️🏼 Nutrients : Canna Bio ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 6.0 - 6.3

Eccoci alla fine!!! Pianta dall’odore spaziale nel pulirla mi ha sballato! Super compatta e molto molto resinosa 💚 Sicuramente verrà riprovata in vaso più grande in quanto ha davvero un potenziale immenso, gradita veramente molto! Grazie a tutti per il supporto ❤️🔥🌲 E grazie a @Exotic_Seed per la collaborazione ❤️

Ein paar Blätter abgezwickt, die die falschen Stellen beschattet haben. Ansonsten nur gegossen und Erdhaken neu ausgerichtet. 22.11. Die Stigmen werden jetzt immer mehr.

10 November 2020 SunSet-Sherbet Well this week is just another week by now, this lady has been threading through the scrog throughout the week and I believe this lady has finished here stretching and is in full on flowering mode now. Normally a plant stretches the first few weeks into flower to stop the growth and use the nutrients it’s getting now is used to store in the nugs and not the growth anymore. Each cola of each branch has reached the scrog net and has a decent amount of light exposure due to the fact that I only has one light for 2 plants and the spread of the light isn’t the best. Started making actual looking hairy heads with the on them 😁 the leaf spread is just getting a lot. Do-Si-Wine This plant is doing splendid due to fact that the long and stretchy-ness of these branches has reached the scrog net above and beyond which makes it a lot easier to thread through the scrog and to get as much light exposure as possible for more thicker nugs 🤓 The heads on this lady has started making THC 😁 the nugs are starting slowly to turn white which is very cool, because the plant is a dark green with WHITE nugs. One branched has snapped which is quiet delicate and grows above the rest if the canopy and causes shadow from on the bottom branches.

Day 122. Job done !!! My first apprenticeship, which was documented here , was a success !!! My friend will smoke his own !!! Not a lot tho ;)))) Day 132. Girls still need couple days to dry out and i am extremely happy with such long dry. I personally in my setup never passed day 9 and here is day 10 and still going ..... Day 135. They spent almost 14 days on strings and in my mind could spent two more, but Boveda packs are in and they will cure for a bit ;) Cure day 20. She is super nice. Will try to do updates very soon. Will update Happy Growing !

germination: Came out strong in 2 days transplanted to 1 gallon first week

Studio54 - DeepEast x WizardTrees.

2020-06-13 Going heavier on nutrients now, I went too high with my initial amount. After pH, it was a whopping EC=2200!! Too much for these ones. I had to dilute this weeks nutrition down, so I have to estimate the nutrition levels as I had to add water and pH it. I started with a whopping 7.6 mL/Liter but probably ended up with about 6.5 mL/gallonI EC=1800/ now keep calimagic constant at 3.79 mL/gallon to help buffer the solution and keep the solution stable. The additional calimagic has stopped the spread of the rust spots and new leaves are a lighter green and have stopped turning dark! Success. 2020-06-14 Thinking about doing the last topping and LST session this week and then transitioning to bloom. Thoughts on that? I have them on a 'transition feed' right now that is very nutrient dense! Started about 950 ppm for the healthier looking plant. The 2nd plant is still getting slightly less nutrients but the magnesium deficiency hasn't appeared in the newest growth. Roots are growing crazy amounts every day! You can see that the 2nd bucket plant started making tons of roots out of the side of the bucket again. Nice to see the bounce back once given adequate nutrition. I have reduced expectations for that plant but it was definitely a lesson in how to prepare and interpret feeding schedules AND how your plant is reacting to them. 2020-06-15 Did some light defoliating today, removing a lot of the lower fan leaves and sucker branches/growth tips... Going to veg for the rest of the week and then change both over to flower. 2020-06 19 Day before nutrient change, they are growing too fast now!! ahhhh!! 2020-06-20 Day of nutrient change! Took them out of the grow tent while I swapped out the buckets.

Growing well and bounced back nicely from the light burn. She’s really packing on the weight. I have 3-4 weeks left I would say based on tric color. I’m assuming the buds are going to swell up even more. Pretty nice sized buds so far and they’re very dense.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

This week the Jack Herer has exploded. The branches have become dense, swollen buds with lots of pistils that are turning orange. The smell has become much more intense and citrusy, however, I think it is further along that it should be. So far this seedling is growing nicely. I hope it continues like this

Flowering day 37 since time change to 12/12 h. Hey guys :-) The buds have developed incredibly nicely this week. The smell is getting more and more extreme :-) . The whole plant itself looks perfect 👍. The growth has slowly stopped as the full energy goes into the production of the buds. This week it was watered 3 times with 1.2 l each (nutrients see table above) They were individually inspected and checked for their health. Osmosis water for the coming week was refilled in a 100 l tank 👍. Have fun reading the diary and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://www.exoticseed.eu/ Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany

29.8. Plants look just amazing! I dont add any nutritions... Just bionova powder nutritions in start which i get from zamnesia 💪 maybe i found right nutritions for my plants 31.8. I will leave those beautys for few days more 😍 i cant wait to taste this buds! 2.9. Another week more and they are done 🤟 so far im impressed 🚀