2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Still extraordinarily green. Buds forming nicely. Beginning to increase the nutrients. Slow and steady, this girl.

Mephisto and bighead going into darkness for 48-72 hrs. Then the chop. Big bomb is the last one standing in the 5x4 will begin flushing soon

Past 2 weeks shes developed dense boulder shaped nugs that run all through out the plant. Today was first day of flush so 5 days ish I'll be harvesting this beauty. Perfect timing for the greenhouse seed company competition that I've surprisingly been giving the opportunity. Happy growing.

This was a simple and easy girl, Everything ran smootly. If smell a visoion was real this would be a great candidate for it!! 😁,, I will be running another set of three soon. 😁

All seems well so far. Had a nice sprout towards the end of week 2. All six plants now how adult leaves. Raised the CO2 levels this week. Plants seem to be enjoying it.

Hermaphrodite

she is moving so fast that i think next week will provably b the last one lets see, for now all i do is water and remove dry leafs , observe them trichomes and that's it, nothing else to do but enjoy my self looking at her. Her color is full on finish and dnt think she will show any new ones, non so ever we can see reds, pinks purples, greens and a bunch more , smell do not at all, cant smell her, mb the rest is absorbing her smell but for now she is the one i grew with lest smell so far ... great for the ones that need discretion not feeding at all anymore just plain water not even ph no idea should be around 7 .0 or something, its rain water <3 <3 <3 As always thank you all for stopping by, for the love you guys put in the community, in to growing and it all, i am blessed to walk among all of you and am blessed with it all. Genetics : AUTO SOMANGO GLUE @ ADVANCED SEEDS All info and full product details can be find in can find @ https://aptus-holland.com/ https://advancedseeds.com/ https://autopot.co.uk/ https://lumatek-lighting.com/ #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #growerslove #dogdoctoofficial With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing in return and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 More info and updates @ https://growdiaries.com/grower/dogdoctor https://instagram.com/dogdoctorofficial https://youtube.com/channel/UCR7ta4DKLFMg2xxTMr2cpIg <3 <3 <3 Growers love to you all <3 <3 <3

These are the Epic Genetics Strains, we have some problem on the begging many mutants and seeds that don't came out, but we get to safe 1 epical widow and 1 Spigol Field, the second Widow on the pictures is the one how after let grow the second leaves, stop to grow up and separate in two for alone, bit know is getting the flowers directly for the stock, crazy because they smeñl fantastic and are complete frosty but they will not become to big.

Vamos familia, actualizamos la quinta semana de floración de esta Amaretto Tarmac de Seedstockers. Empezamos abonando ya con varios productos de la gama Agrobeta para la floración. Temperatura y humedad dentro de los rangos correctos, 12 horas luz, 12 oscuridad. Una lástima que de todas solo aguanto una, y en concreto tiene un color espectacular aún así seguiremos con el diario hasta el final. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

So no change just got fatter buds. The 2 on the ryt are being flushed for 7.10days. 2 on the left are different strains. Had a few newbie problems at the start but soon realised its a weed n if u listen to her she will let u know what she needs.. Been a nice journey and carnt wait to dry n try. Happy growing peeps

Harvest time for Divine storm. Not much to say about this strain, except this is my last time growing it. July 11 update, 84 grams dry weight. Same taste and smell as before, a wine taste with a bit of fruit.

Preflowering, maximizing the sizes

Love this plant! Will definitely grow

Amazing week for my girls they doing very well I'm so happy and proud of them. I make some clones from radical juice, washing machine and bubba kush✌️ stay tuned for more 😊 it will be amazing grow 😁

Veg15, han crecido como 2 centímetros desde el transplante, aún no necesitan riego, desde ahora regaré cada 3~4 días, cuando el sustrato este un 80~90% seco, han estirado un poco desde que subí la distancia del LED y bajé su intensidad, mañana haré Main Lining. Veg16, hoy se torturaron las plantas con Main Lining, se cortó arriba del segundo nodo y se cortaron también las hojas de un dedo bajo el primer nodo, las plantas que tenían suficiente crecimiento de les hizo LST también, cuando se apague la luz se efectuará un spray foliar con B-52, y mañana un nuevo riego. Veg17, riego con 5.5 pH y EC 1.8~1.9, se regaron con 1.5 litros y no se obtuvo drenaje, próximo riego tendrá que ser con más cantidad de agua para obtener drenaje, el agua se calentó a 23 grados y se oxigenó antes de regar, mañana se ajustará el LST ya que siguen creciendo muy bien. Veg18, hoy solo se ajusto el LST, siguen creciendo bien. Veg19, ajuste de LST, nada más que anotar. Veg20, siguen creciendo, talvez hay que ajustar la cantidad de luz DLI. Veg21, tuve que poner una estufa para aumentar la temperatura en el indoor y bajar la intensidad del LED a un 50% para alcanzar un DLI óptimo. Hoy haré ajuste de LST y revisar la humedad del sustrato.

07/28: début de pré-flo je vais tester bactohemp avec c'est acide humique etc dans le prochaine arrodage/engraissage .... #29 07/29: 1L *avec une cuillère à thé de bactohemp. Espérons que ce première arrosage de 1L vas régler mon problème hydrique... prochaîne arrosage avec runoff. J'ai ajouté des tuteur pour que le plants donne plus d'énergie à faire grossir les buds que à porter leurs poids #30 07/30: bactohemp a eu un effet bénéfique il me semble que les sites floraux se mettent déjà a grossir :) #31 08/01: premier arrosage de 3L*engraisser avec "runoff" :), l'étape la plus stressante a mon gout quand grow des autoflo en indoor #33 08/03: grosse défoliation aujourd'hui #35

Sorry brothers to be so lait this week... had some problems with may Home Assistant raaspberry. Lost a day and half of photos. Weather is Getting better Black Cream Is in senescense. Buds are fattening and getting some color. Shes so Beautiful now. The other are starting to a lot of pistilis out. Flowering in early stage. didnt train them so much let them grow more vertically. Chears BrotherHood