All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Hi everyone, masters and beginners. This week we will use the fimming technique on these beautiful photoperiodes to promote more gems. Thanks to the Fimming Technique, The Growth Hormone Reaches All The Side Ramifications and Not Only The Main One of the Central Stem. Stay on the piece for subsequent updates

Week 9 commence flushing. This lady is looking great, super frosty, sticky and got a sweet aroma. Removed some dying leaves and now we wait for chop in about 6-7 days.

Well I made a few upgrades since last week. First I went ahead and invested in a nice A/C unit. I also, improved the exhaust setup. I went ahead and took a plant out and germinated another ( I wanted to add a different strain. Lol). The ladies seem to be doing pretty well. They are all on their 4th or 5th node. I am waiting until the 6th node and then do thier first topping. Thanks for all the support! 😃😃😃

Deepforest Super auto is doing good. She has gotten a lot stronger since she found the solution. Everything is looking really good at the moment. Thank you Doctor's Choice, Athena, and Spider Farmer. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g.

she grows very nicely takes water and nutrition well. I have started to train it and it responds well to that.excuse the lighting a get home the lamp for the photo and dry cabinet so there will be good pictures outside the tent too

10-8-2024 TOP DRESSING AND WORM TEA..... NOTHING MORE 10-9-2024 22ND DAYS INTO FLOWERING SMELLS CRAZY ON THESE STRAIN GETTING SOME SNOW ON TOP LET'S GO 10-11-2024 DO NOT HAVE ANY PEST BUT JUST IN CASE NATURESGOODGUYS HOOK ME UP WITH LADYBUGS JUST GOT HANGING PATCH ABOUT /WITH SHIPPING 10-14-24 NOTHING TO DO...JUST WATER (PH6.6)AND WATCHING THE PLANTS....... COPY CAT GENETIX STRAINS ARE CRAZY!!!!!!!!

expanded the pot patch to include some companion planting and cover crop this year. Added 2 yards of grow max composted soil mix from the local garden centre. now 18" deep and I will be adding some leaf mould, myco and a super microbe tea to kickstart things. ************UPDATE************* due to the shit weather in Canada this year I have decided to NOT plant an outdoor crop this year, due to late season start and big el nino which means lower than norm temps and lots of rain for us which = issues growing.

, .:: WEEK 04 ::. Day 24 - 24/05 - Watering w/ Alga Grow + Rhizotonic, 1 L/plant (I forgot to take photos) Day 28 - 28/05 - Watering w/ Alga Grow + Rhizotonic, 1.5 L/plant + Defoliation of the first stage and leaves that block light to secondary branches + LST Day 29 - 29/05 - First signs of flowering stage

Day 120 PH 5.8 EC 0.2 PPM DLI 6h PPFD/ Water 18c Day 121 Tue PH 6.0 EC 0.2 PPM DLI 6h PPFD/ Water 18c Day 123 PH 6.0 EC 0.2 PPM DLI 6h PPFD/ Water 18c Day 125 PH 6.0 EC 0.2 PPM DLI 6h PPFD/ Water 18c

En esta etapa no agregue ningún nutriente a la solución para evitar que la planta tenga gusto al nutriente, así dicen los expertos que queman esta hierba. Yo lo hago solo por deporte, no porque lo fume. mantuve el ph en condiciones estables a como dictan los científicos en este rubro. la próxima😁 semana corto las plantas y las pongo a secar.

Nothing important to say... she s growing up strong ang healthy outdoor

Rainbow melon has stolen my heart From day one the plant was bigger as nice strong stock handles low stress training well some of the biggest buds I’ve ever had. I am truly in love with this plant fade is absolutely one of the Most beautiful like an Indian summer Cannot wait to see what they come up with next💪🔥💯

En está semana la GorillaKingAuto ha ido super bien, tiene my buen color y la veo muy bien. Sigue votando pelos blancos, en está semana le empezare ha usar un fertilizante de floración de la marca de boom Nutrients 😍

PABLO ESCOBAR / DIVINE SEEDS WEEK #8 OVERALL WEEK #7 VEG This week she growing at a good rate I continued to top her her and use the BUD CLIPS for LST to open her canopy she has responded well to the training she's been put through!! Stay Growing!! Thank you 😊 for stopping by and taking a look it's much appreciated!! Thank you DIVINE SEEDS!! BUDTRAINER.COM BUD CLIPS PABLO ESCOBAR / DIVINE SEEDS

So I am starting the tiny potter grow for the contest. Hopefully the seed germinates quickly, and grows well. I scuffed the seed as seen in the video. I then placed it in a cup of water. I will be putting the cup of water with seed in it on a Spider Farmer germination Mat with controller. To hold a steady 80°F. When she sprouts I will place her in the grow space. There she will be under the Mars Hydro FC4800 light in my autoflower room. Thank you Mars Hydro, Spider Farmer, and Seedsman for everything. 🤜🤛🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g Spider Farmer 10X20 Heat Mat Kit - https://amz.fun/lsa0J Spider Farmer Amazon Store: https://www.amazon.com/spiderfarmer Spider Farmer Official Site: https://spider-farmer.com  Coupon Code: Saveurcash (stackable)

Astalavistaa

Added some CO2 to help with photosynthesis while at higher temps. These ladies really blew up at the end of last week and this week! The smell has really starting to be noticeable and pistols / flowers are finally showing! Side note I finally got more seeds to replace the one I lost early on. These ladies are really starting to become a treasure. Ended my LST this week.