Did a lot of defoliation, there were just too many leaves. Set up an auto watering system which is in manual mode right now Plant is very happy! Trichomes starting to form

Week 5🌸 done, first day of week 6 They are developing beautifully Studio & Rainbow Gelato are heavy stinkers - sweet + gassy It seems like Bruce Banner in the back could get more feeding Will up the volume of water a little in the following week I take out a few leafes here and there, don’t seem to stress them a bit At nighttime it gets pretty cold, 16/17 degrees so some of the pistils tops (Blütenstempel) are getting dark

They r going! I definitely don't reccomend the strain Bloody Skunk from Sweet seeds to anyone Not well stablished tons of problems

Beast Mode Time!! This thing is Huge somenone should tame her with my SFV

May 31st Everything is looking good after the LST from last week, I said I was going to swap them into flower and I realized that I should wait another week or so as they will be going into a new home so to speak. Updates coming next week for that. Doesn't look like to many PH issues anymore or whatever was happening but the plants seem a bit happier now June 1st I Figured today would be a good day to do a bit of defoliation on these girls in order to get some more light down to the newer growth. I didn't take a ton off as you can see, but for the size of the plant I suppose it was a decent amount. I read somewhere that a general rule of thumb is only defoliate roughly 30% of the plant at most. I'm not to sure if it is true but I tried to stay within that general rule. June 2nd, I decided since I would have them in veg for at least a little while long I would top them again. After topping I gave them some water and nutrients. I know I said I would do straight water for the next one but I went for it anyways as it seems the flowering mixture may be giving it some deficiency's. So I gave them the recommended feeding for heavy veg growth. I gave about 3L to the bigger plant and around 2L to the smaller plant (as it was still a bit moist but it wasn't damp). My crappy soil tester was saying my soil was at around 8PH so I figured I would aim to feed around 6ph. With the small amount of runoff from the smaller plant I did A PH test and the PH seemed to be around the 4.5-5ph area. My liquid ph meter arrived broken so I am waiting for the new one, therefore I am using the general hydroponics liquid test kit. I presume my soil ph is lower then the water I have given seeing as it went from 6ph water to around 4.5. Next feeding I will aim to feed at 7PH. With the defoliation I just made sure that the tie downs were decently tight still and moved what I thought needed to be moved. June 3rd,4th Not a ton to update on. I didn't get any pictures for these 2 days. June 5th Some obvious problems going on with the bigger plant. I am guessing it is the PH issue but I am still unsure. The one browned leaf I accidentally squished in the tie down while I was low stress training it so it got a bit damaged. Other then that the plants are growing decent. Im not sure why I didn't have more pictures for today but I mainly took problems of the problem areas. June 6th Not much going on still. They are still just hanging out not doing to much. It was a busy work week so pictures in general were a bit lazy. I will try to get more for the upcoming weeks. Big news for next week!

At this point I’m not doing anything to the plant besides making sure it gets nutrients

Uma planta estava muito travada e pela minha experiência não vale a pena o custo da manutenção dessas plantinhas é chato mas preciso do espaço e do tempo para cultivar plantas que deem resultado. Coloquei um gancho leve em cada planta hoje para ver se aumenta a área verde.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Just a meme dump diary.

😬

Day 35. I know its last day of previous week, just didnt wanted to mess with week type. Its BIG week . New light was added and rhythm changed to 12/12. Pot was top uped with 3 handfulls of BioBizz Worm Castings. 1.5 g of Biosys and 1.5 g of Great White were added too. After training she got 2.5l of water, fastest drinker at the moment. Girl got small defoliation, when you bent main top, one fan leaf is always staying in everyone's way, so he had to go. Think to do big clearing on day 14-18 of flower. Got first wave of LST, main top was lowered, couple shoots were repositioned and secured too... Girl is a racer, double the size of others, thinking to scrog her even ... Still get nitrogen excess and magnesium issues, girl is on plain water, but pot is still too rich and i added castings ;)))) TS1000 by Mars Hydro, great light, did perfect job during veg period, now i added one more for better coverage of long tent. Think in tent 1x1m i could manage to flower all 3 without any ad ons .... Great small light for amazing price. Day 37. I see more progression, went with 3.5 liters of higher ph 6.5 , run off was 495 ppm, 6.3 ph, so all should sort out .... i still believe its more overdose than lack. She drinks fast, was even droopy a bit, needed watering few hours earlier... Day 38. Looks like higher ph and run off helped her a bit, anyways still see some damage, but form of even canopy compensate everything, she is very nice ! Day 41. Girl got heavy training, 5-10 leaves were taken off, most shoots secured. One leaf was double ;))) 3.5 liters of 6.3 water went in. Happy Growing !!!

eating 5mL A+B daily change Sunday

Ya están bajo el Led 300 en crecimiento. Última semana de vegetación.

Day 28 flower, things are doing well, humidity and heat are stable, plucking off leafs as I go along now anything blocking bud sites. I'm on week 7 of the biobizz feed schedule full dose, these girls not showing any nute burn or stress so far either I could probably push them more. Day 33 from flip. Heat and humidity are under control. Sitting at 27°c and 40-47% humidity with lights on. And 20°c and 47% humidity lights off. Ph levels on feeds and watering is 6.5, there really loving the biobizz range and the marshydro tsl2000 combo. So today the plants are getting fatter and nice and dense. Its super sticky and stacking on trichomes. These 3 beans came from The same pack, and each one smells diffrent. Closest pheno is a really lemon citrus smell and its strong. Middle pheno is like a sherberty smell and really tickles the 👃. Pheno 3 at the back is like a lemon skunk, really pungent and its got the fattest buds so far too. Day 34 flower, Feeding molasses today in 6.5ph water, I will start the next week tomorrow and will be starting on week 8 of the bio bizz nutes schedule. Thanks for all your support so far. Keep on growing

week 9

Hopefully she continues to fatten up! Can't wait to see the final result but still a fair bit to go.