9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

A bit slow but healthy and cons The Vally Fruit is 3 days younger than the Cherry Dream and this are the best of each grouo Disclaimer I don’t blame the seeds at all they were all nice and good split I just had a bad roll of the dice I do think I’ll have one for a fair sample of the cross

🚨 CHOP DAY!! 🚨 So my Northern lights by @growerschoiceofficial has finished her life cycle at 79 days old. Upon chopping all the girls this plant is prob heavier than the other 3 put together!!! She did amazing in flower under her new light by MarsHydro the tsw2000!! Can't wait to see how this light treats the girls through the whole life cycle of a plant. 💡light sponsor💡 @marshydro_amazonled @marshydro12 @marsnier #marshydro #TSW2000 Low stress training clips- @madmadameplant @madmanplant Www.madmanplant.com Other companies in this grow- @foxfarmsoilandfertilizer - 100% ffof @acinfinityinc - fabric pots @generalhydroponics - flora trio line @vivosun.official - inline exhaust fan @opulent_systems - 4x4x80 grow tent @growerschoiceofficial - 1 Northern lights seed @inkbird_official - temp and rh controls Well that's a wrap 4 this grow!! Till next grow growmies best of luck and Happy Growing!! Stay Zooted!!🌱💚💨🔥🔥🔥👊🏼🤟🏻

04/18/2021 Bluedreamatic is cruising along, and smelling good, a good berry smell. Spray with microbial tea to boost immune system there is some PW showing up hope microbial tea has the bacteria/fungi to combat bad stuff Also a soil drench of (MICROBIALSCIENCE) Seed & Plant organic starter

I went ahead and transferred this week I also decided to start up my out door project things are looking good clones are Adapting well to new systems

Day 65 : officially 1st day of flush . Changed the reservoir with fresh water and 1.2g/5 gallons of Yucca extract and 5ml/gallon of Cleanex from Botanicare . Day 68- I did some defoliate. Few more days until harvest.

This fine lady is really starting to add some nice colours due to some cold temps in our neck of the woods. She has a ton of trichomes development now! I am really impressed with the look of the buds. They aren’t all full of leaves which should make trimming her a pleasure. 😎 She has really been fattening up this week. I am guessing she needs another week or so to finish up. Her colouring should be quite something once she is done. 🤩 I am still having the odd spot of powdery mildew pop up but nothing too serious. I have been treating it with greencure every 3-4 days and it’s keeping it under control. I haven’t found any affecting the buds yet which is a win! This is the reason a good effective regimen of treating plants for pest and mould/mildew is an outdoor growing must!! I have yet to lose a plant to mould or peat so I swear by it. I’ve seen a bunch of my associates with some powdery mildew beast. Such a shame to see😱 Stay tuned for the final week 😎🍿

Hey guys I am a day late on the update cuz I went away for the weekend to a buddy's bachelor party and ended up with a broken clavicle in two places and need surgery tomorrow. I dont know how I'm gunna keep things rollin here but I'll figure it out. Anyways I couldn't get the plants out of the tent tonight but when I have some help tmm I'll try and get some better individual pics. I fed them before I left Friday at 1110 ppm with the green buzz and got a little burn, which is a good thing because that kinda shows me that less is more with this line and may save you a few bucks over time. It's also nice that there isnt 11 different bottles to pour from so far I couldn't be more impressed with GBL. The planta were all pretty bone dry today but look better than ever. Pics were taken at day 29 this week, temps have been around 73°F and 58% RH. This tent is in a basement so even with the dehumidifier the rh is pretty high especially with the lights out but the plants seem to enjoy it and I have plenty of air circulating This one might be a keeper if it fills out strong, shes a bit more structurally sound than the first pheno fatter leaves, thicker stalks but same stretch. Edit: I fucked somethin up here in the video I put day 29 bloom, these were flipped on june 9th and got 36 hr dark period, I uploaded the video last monday so it was actually on day 22 flower not day 29 but it's a little late to edit the video so ignore that day and I'll make a new one tonight which is actually day 28

7/11 Got half in of rain last night. Glad I didn't water. Ph of rain water is very acidic. Added supports to the blueberry cheese in the 50. I just used string and went diagonal and attached to the cage. Wrote a ton but it disappeared. Anyway looking at previous diaries I was wrong about senescence. It wad the life cycle of those earwigs that did that to my plants (see other diaries). This soil mix is amazing. These plants gave only gotten like two small feeds of big bloom. I showed my commercial buddy and I'll keep it between us what was said but it made me feel really good. I'm considering either expanding the cage in one direction with some lumber I already have or moving a couple outside tge cage. That way I have more room. He said he's seen plants structurally similar reach huge heights (14f) so I'm just trying to avoid future problems. I at least need to get the trellis on. I hope poor that's food enough. Did a real nice video this morning but nothing wanted to upload and it just uploaded the same one twice. I'll try again and hopfully it will go up. If not I'll put it up tomorrow. 7/12 Dad has surgery this morning. I did a quick video and took some pictures. I need to do some rearranging with the plants and cut a pallet to fit in the back. Then I can put a vertical trellis up. Haven't decided if I'll add on to the cage, remove a couple plants or just rearrange things but I'm leaning towards the later. They will need water again and I'm wondering if I should start nutrients but everything looks so good. By this time on past diaries I'd be losing all my leaves bu now. MI think it wad part ear wigs and part to many nutes. This soil mix is taking these plants through veg. I'll probably do a feeding soon but it will be organic and it will he small like a big bloom or ancient amber. Im leaning towards not using growbig this year as my plants seem to be doing great without it. We'll see. I'll keep this updated. UPDATE: Went back over and gave plants a full watering. Some were slightly drooping. I was going to add nutes but decided against it as I didn't see any deficiency. So far NO Earwigs! I comed through the plants and I did find a jpn beetle which I happily killed. I mixed up 8 gallons of water and gave it to the 11 plants so it wasn't quite a gallon a plant. I need to rearrange the plants so I have room to move around. I also need to cut another pallet and use the spaces I'm not. Trellis needs to go up. 7/13 I think I've got the watering amount down. Now just to find how often which will depend on weather. I've watered very little this year. They loved that gallon. I was going to use big bloom and kelp me/you but looking at my garden I decided not too. My buddy asked what i was addingvthings for and to wait fir what i added to do what I wanted it to do. I see no nutrient deficiencies so why add anything? I think this soil mixture will get me all tgexway through veg. I dont plan on using much in fliwer either. Definitely good genetics. I really need to cut that palley and move the 1 10gallon to the far back coener. It will open thibgs up so much better. The garden looked so beautiful this morning. Getting very aromatic. I started untangling trellis netting but had to leave. I'll update what I do. Happy growing folks UPDATE: Went back over as I had a slight intuition that I needed to check the garden. I found and killed at least 7 jpn beetles. That's what's been making holes. They tried to escape and bounced off a tarp but I got it. I went through the interiors and found two pillars. If these beetles are gonna be around I'm ordering a net. I'm also close to positive I'm going to extend my cage in the back a few feet. Things are getting unmanageable in here. My buddy said I'll be having problems soon if I don't do something. I'll document what I decide to do. 7/14 Found ONE jpn beetle in the garden this morning. I can manage those well enough. The birds help too. I check my garden multiple times a day so I manually remove many pests. However I've noticed some thrip damage on a leaf and a leafhopper damage on "A" leaf so there are "some" pests around but not enough to spray shit. A couple wasps were doing there job while I was there. Supposed to get thunder storms after three and tonight. Supposed to get over a half inch of rain tonight. I lifted the bags and decided to hold off and let mother nature take care of it as the bags weren't totally dry. Only problem I really have is space. I AM moving that 10gallon (that's the same size as some 20's) in the back. There's 27in not being used and a few feet the other way. My buddy cautioned me that I'm going to have problems since my plants are so crowded. I agree with him. I spoke with me father and we have most materials to extend my cage four feet in the back. I think that's my plan. I'll extend the structure before the stretch then I can put up the supports. We'll see how this goes. UPDATE: Went back over to check the girls as I had a feeling I ought to. When I got there I saw that a couple of the blueberry cheese were pretty light (liftng the smart pot) but the others seemed to be fine. ONE 10th planet was light like that and the purple punch in the 10 gallon was as well. Each plant thar needed it got at least a half gallon of water. I'm waiting to see if we get the thunderstorms and the half inch of rain. I watered the MASSIVE blueberry cheese in the 50 but I only gave it 1 pitcher which is like 1/4 gallon or so. Don't know why I even gave it that. Looked fine but the soil WAS pretty dry. Next year I'm giving myself way more room. I was running trying to chase these jpn beetles. This time I have the dawn and water and a measuring cup to knock them in. This ain't my first rodeo. I did notice some bright yellow streaks on a leaf edge and I'm hoping it's not septoria. I doubt it but I have an anxiety disorder and I worry. I hope I can get the cage extended sooner rather than later. It's getting hard to move in there and more importantly I can't take any more plant pathogen problems. I'm considering starting a plant doctor regimen just to be safe. 7/15 Got a bunch of rain last night. No jpn beetles in the garden and not really much damage. I did notice this (I think it's leaf hoppers) that leave those dots close together on a leaf so it appears I've got a variety of pests. I'm considering how to approach this. I mean the damage is very minimal but I don't want it to get out of hand. Another thing I need to look out for is leaf septoria or any other fungal pathogens. I believe that has been part of my problems in the past. I think that's what caused my earlier grows to drop all their leaves so quick. I think I'm being overly cautious but its very crowded in there. With my father just getting out of surgery the girls will need to wait a little bit before before I can extend the cage. I could still cut the pallet and move the 1 10gallon and that would give more room. I rearranged a LITTLE BIT so they have a little more room but I've really got to get this cage extended. UPDATE: IT Rained so hard I had to pull the car over. We hydroplanned the whole way home. After working ob my house I went to see what the damage would be like. NOT A SINGLE BREAK THAT I COULD SEE. I took a video but since the wifi here sucks I'll have to upload it tomorrow. Walking around in the cage even if I cant get it extended I think I'll be OK. It obviously will open me up to lots more issues but at the very least I can reorganize before I build on. The 10 gal purple punch would fit perfectly in the back and I have a pallet I can cut to fit it in place. Putting that one back and pulling the others forward will be much better than what I've got going on now. I'm also thinking about running an extension cord and putting fans under the canopy or at an angle to keep the wind moving. Just thinking outloud. However after that storm the girls looked as happy as I've ever seen them. All happy praying to the sun, thankful for the much needed rain. Mother nature does a pretty good job with out me messing with it. I've noticed a couple interior lowest leaves turn yellow and die like a nitrogen deficiency but everything else is fine. Also noticed a leaf that looked like a p deficiency but again, it was the VERY bottom leaf on ONE plant. Again the rest of everything looks fantastic. I'll keep an eye out for anymore nutrient deficiencies and if anyone that reads this sees some please let me know. I should've taken stills since they looked so good but I got it on video. 7/16 It POURED last night and throughout the day. TORRENTIAL rain. The branch breaking sheet rain that us outdoor growers learned to fear. My plants aren't trellised currently. I know what I need to do now. I have a pallet to put in the back corner and I'm moving the purple punch in the 10 there. And pulling others forward where there's more room. Then when I extend it (it's gotta be done this week) everything g will be in their proper place and I can just throw on a vertical trellis. I also noticed more (leafhopper) damage on a leaf. Different leaf of the same plant so I'm considering spraying something. I have a number of products but I was trying not to use them. Luckily I have these diaries so I can look back and see certain plants reactions to certain nutes or fungacide/insecticide/nutes and the doses used. I haven't been using much but if my plants will remain cramped I'm going to start the plant doctor. I'm seeing more pillar damage too but BT is super narrow so I'm thinking when I fo spray for pests I might use cap jack and be done with it. Then I can apply the BT in flower if it's necessary. I took a video but I have to wait until tomorrow to upload. I took a quick snapshot though. 7/17 Despite the torrential rain I don't have any breaks. I'm noticing more pest damage though. Another leaf on the same plant had those closely shaped round circles. I forget what pest it is but it's there. Caterpillars are there I'm sure so I may do a preclcentative spray. Just unsure what I'm going to use. I lost a COUPLE very bottom interior leaves that look like they just got used up. After this rain I think think the plants might benefit from a feeding. Probably next water after they dry out. I REALLY need that cage extended. I expressed that today and it should be done this week. I'm looking for pallets today. I have the little one that I can put in the back which will allow me to move the 10 gallon and move the other forward. That will help some but I need more room. I'll update as I go. UPDATE: GOT A SMALL PALLET AND IT FIT PERFECT IN THE BACK ROW. I MOVED THE PURPLE PUNCH IN THE 10 GALLON ONTO IT. I SHIFTED A BUNCHVIF THINGS AROUND. I ROTATED ON BLUEBERRY CHEESE 180 DEGREES SO IT WOULD FIR BETTER. CROP ROTATION IS GOOD ANYWAY. I TOOK VIDEOS AND YOU CAN NOW SEE THE ROWS MUCH BETTER. 2 WITH 3 and 1 WITH 4. IT'S SIGNIFICANTLY BETTER THAN IT WAS. I CAN GET AROUND ALL SIDES OF EVERY PLANT NOW. GRANTED THE LARGE 50 IN THE BACK IS GOING TO HAVE SOME TROUBLE BUT ILL JUST STAKE IT TO THE CAGE. IT WILL GROW TOWARDS THE SUN ANYWAY. IM SUPRISED I DIDN'T LOSE A BUNCH OF LEAVES AFTER THIS RAIN. MOVING THINGS AROUND AND LOOKING ON THE INTERIOR OF PLANTS I FOUND A COUPLE LEAVES THAT HAD BEEN USED UP. I REMOVED A COUPKE LEAVES THAT HAD DONE THEIR JOB. I'LL UPDATE AS I GO ALONG.

Super proud of the way the manifold is turning out. So symmetrical! Between now and the next update I’ll be doing some defoliating. Get rid of some of the lower fans, create some airflow up top, and possibly remove the lowest set of growth tips on each of my main stems. Plant is flourishing and healthy but those leaves could maybe be more vibrant. Might be time to start introducing nutes to the reservoir. Edit: Added a video of the plant after defoliating. Ended up removing the bottom 2 nodes of growth except for the outer fan leaf on the top one. Also moved the soft ties up one node and adjusted where they’re anchored so they pull the stems apart more. Can’t wait to see what she looks like in a week!

Google doc for nute schedule: https://docs.google.com/spreadsheets/d/1HtHnZOxMHtBK5DFQjpBun2lbhDxYVhI7lJaq4W6xbyk/edit?usp=sharing (Choose Sweet Seeds at the Bottom) EC Target 1200-1500 Red Poison is getting the same treatment as everyone else, but showing curling tips. I'm almost positive this is genetics bc none of the other plants are showcasing any sort of issue - therefore, we move forward. GROW BABY!

4.21.25 3rd week of veg tie down method going good. Definitely going to have plenty of space for light. Hoping to get couple more weeks of veg in before she’s flowers! Time will tell very soon! Thanks for checking out grow

Has a sweet taste to it. Again , I didn’t log any larf in the weight. Very resistant strain.

This beautiful girl turned out to be a real strong queen who withstood very strong stresses, she is very persuasive for ScrOG, in 2-3 days when she comes out of the stresses I will switch the light cycle to 12/12.💚💚💚

All going very well. They have a crazy stretch on them. 18.3.23 Turned tap back on when lights came on 22.3.23 Gave all 7ml pk 5-8 3ml acti Vera, and gnatrol 4ml bio heaven and recharge each in 1L water from top Ph 6.1 Ec 3.0. Ppfd around 900 Had to put pineapple chunk outside. She got pushed out of the room watering from the bottom like in room Mont Blanc 56cm Blue sherbet 94 +75cm Purple punch 96 + 100cm kush mints 106cm

Really impressed with the purple peanut butter breath pheno she gets darker everyday, still mostly clear trichomes. Pbb smells like lucky charms marshmallows rain of frost has some sour tropical funk and diesel and the afghani has a classic hash smell spicy

Her first week!! Soil mix in her little 1L airpot is 2:1 Soil:Perlite

Hello people, we are here with another update day 34 for all these lady's. We currently have two shorty's and 2 tall girls. I'll be doing plain water with carboload from from now till the end of flowering Peace and love ✌️✌️