Start by saying 2 are in flush now! (Black cream and sunset sherbet) I used flawless finish on them and then water from then on! Started to see some fades within a few days with the black cream and sunset sherbet leaves have lightened up, just awaiting some fades! And I’m watching under a 50x loupe to aim for the perfect harvest! The aromas are crazy in there got all sorts going on until I touch one and identify it’s aromas but as expected everything is going to be full of terps and resin! On the end road now! Flush next feeds for the purple punch and forbidden runtz! I’ve added a picture of the royal creamatic I took out as it’s just jumped into flower! Glad I took her out now too as she has some coverage and I wouldn’t want to lose no light at this stage! End result with dynomyco! IS… use it highly recommend. I’m using it in all my grows from now on! It’s a great additive providing your plants with a lot more beneficial bacteria that aid the myco and allow the plant to uptake nutrients better and gain a lot more stability so it’s a must! Contest or no contest I’m glad I got to use it or how would I have known! Thank you Ari 🙌🏽🙏🙏🙏💚

I pour the beet by Hand. Every two days like 5%-10% of the total soil amount. Today (12.5.) is 28.Day of flower (55-70 Days total) Lamps are on 600w the top of the flowers have 600-800ppfd Vpd is 1.2-1.3

Welcome to the diary of the Limited edition strain: Orange skunk ryder OMG, this DANK has started to finish it smell like i have at least 50 of them in flowering, but when you follow your nose you only find this little lowryder crossbreed. it looks great, trichomes look very tiny like grown under LED, but it's outdoor i'm leaving this outside for some extra days and then i'll start to cut the main cola, which isn't really big, but smells o so promising. this plant is very stealthy, and it needs to be stealthy because it's that stinky.

Day 92: what’s happening guys! Week 14 is here. Temps have been okay and humidity has been okay. I’ve got the 400w hps set up and it’s dropped temps slightly and slightly raised humidity which is what I wanted. In my opinion she’s looking ready for the chop! I’m seeing plenty of cloudy trichomes and a few ambers and I’m going for less of a couch lock so I’m thinking around now she is ready. I started plain water 6 days ago and she’s recieved 2L,1L and then 1L again today. All leaves have started yellowing, I imagine this is the plant pulling the nitrogen from the leaves as there’s none at the roots. How much longer does she need to be fed plain water for before she is ready for the chop? Thanks! Stay safe and happy growing✌️🌱🍁 End of the day edit: I decided to harvest the majority of her. The bottoms just a bit under developed so I’ll leave it going a bit longer. After chopping and taking photos out of the hps light the nugs are looking a tinge purple although I can’t see it as strong with my eyes. Hopefully I’ll get a wet weight later so I can add it to the diary later on. Temp is around 24c and humidity is 45-55. How does that sound? I’ve got extractor at the top and a fan at the bottom. We’ll see how long it takes to dry, hoping to go for atleast 5 days. With the virus going around I’m really making use of what I have at home so hopefully the climate will hold up🤞 any tips and advice about drying and curing etc is hugely appreciated🙏 I’ll be back with the harvest update once she’s dried out✌️many thanks happy growing!🍁🌱 She weighed in wet at 110 grams. Any ideas what she’ll weigh dry? I’m guessing an ounce if I’m lucky.

After three weeks in the solo cups I transplanted to 1 gallon pots and three days later I noticed yellowing on one of the plants so in 16oz of water I mixed in 1/4 tsp of Epsom salt and gave it to both plants in fear of magnesium deficiency but the other plant is thriving and growing strong. YouTube video:

unfortunately I had trouble doing my LST and a branch broke while trying to fight against the tension. I changed my tent this week, the lamp and the Mars Hydro extractor convinced me that it was good gear so I took the tent in + 😀 Pulling all the branches with ropes is way too much work... I think I'll just pull the 2 main branches down and add a scrog net next week. ----------------------------------------------------------------------------- -Water: tap water (280ppm). I add 0.7g/L of Grow mineral powder and 0.2g/L of Calcium to reach 820ppm and I adjust the ph to 5.8 -Daytime temperature: 29°C -Night temperature: 25°C -Humidity: 45-75% -Lamp: Mars Hydro FC3000. intensity 60% at 40cm from the top leaves -Room: Mars Hydro 100x100x180cm -Extractor: Mars hydro 402 CFM Max. power 2/10 -Substrate : 70% coco, 25% perlite, 5% vermiculite.

11/14 Day 23 from flip. The stretch has been explosive, so much so that I’m beginning to have height concerns. I can probably add another trellis net and lower it down a little bit, but not by much. I just have to hope they level off soon. I’ve needed to turn the lights down every day or two, and even then I’m at almost 800 PAR / 34.6 DLI at the topmost part of the canopy. The majority is closer to about 725 PAR / 31.3 DLI so I’ll leave it there for now. 11/19 Day 28 from flip. Well, the stretch sadly hasn’t stopped. I was able to raise my lights a few extra inches but it’s now maxed out at the top of the tent. The topmost branch is about 12” away from the lights so this really is about as much as I can realistically handle. I realize Ethos was up front about sending this strain into flower earlier than normal due to the way it grows but this is really nuts. We’ve now easily tripled, if not almost quadrupled, in height and lord knows how much there is still to go. Stretch aside, everything else with the grow is bang on. Everything looks healthy, no signs of any disease or pests, every branch has solid airflow around it with nothing being too crowded. I just need the stretch to stop and everything will be great.

🗓️ Veg – Week 4 complete (2 days late 😅) Nothing but good news this week: The 15L pots are already fully rooted after just one week – much faster than expected. The plants are looking healthy and full of energy 🌿 I’m still going very light on the nutrients – currently at about 50% of the Advanced Nutrients schedule. Still, they’re clearly starting to take in more – you can feel the momentum building 🚀 Regular LST has also paid off – the structure is opening up nicely, staying compact and well-exposed to light 🔧🌱 And here’s a major milestone to wrap up veg: I switched to 12/12 right at the beginning of Week 5 – flowering phase is officially on! 🌸

Keeps going. Etsy seeds coming through. I think soon in the grow a cal mag problem comes up. Chime in when you see it.

Greetings, fellow growers! Let me share the latest progress of my LSD-25 Auto journey as I navigate through Weeks 9 and 10 of the grow cycle: 🌟 **Vibrant Bud Development**: My LSD-25 Auto plants have entered the final stretch of flowering, with buds reaching their peak size and density. The once-promising flowers have transformed into resin-coated colas, exuding the characteristic psychedelic aroma that hints at their potency. Trichomes are abundant, signaling the optimal cannabinoid profile and a potential journey into euphoric bliss. 📏 **Fine-Tuning Environmental Conditions**: As I approach harvest, my focus remains on fine-tuning environmental parameters such as temperature, humidity, and airflow. By carefully managing these factors, I aim to create the perfect microclimate for my plants to thrive and produce their most potent and flavorful buds. 🍃 **Leaf Senescence and Nutrient Management**: In these late flowering stages, some leaves may naturally begin to senesce as the plant reallocates its resources towards bud production. This process is a natural part of the plant's life cycle and signals that harvest time is drawing near. Meanwhile, my nutrient regimen continues to be meticulously adjusted to ensure the plants receive the essential elements needed for robust flowering and resin production. 🔍 **Trichome Assessment for Harvest Readiness**: With harvest approaching, I'm closely monitoring trichome development to determine the optimal time for harvest. Using magnification tools, I inspect trichomes for signs of maturity, aiming for a mix of cloudy and amber trichomes to achieve the desired balance of cannabinoids and psychoactive effects. 🌞 **Preparation for Harvest**: As I prepare for the culmination of my LSD-25 Auto grow journey, I'm gathering the necessary tools and supplies for harvest day. Trimming shears, drying racks, and storage containers are being readied, while my workspace is meticulously cleaned and sanitized to ensure the preservation of my precious buds' integrity. Proper post-harvest care and curing techniques will be employed to enhance flavor, potency, and overall quality. In summary, Weeks 9 and 10 mark the final stage of my LSD-25 Auto grow adventure, where careful attention to detail and precise cultivation practices pave the way for a bountiful harvest of potent, psychedelic buds. Stay tuned for the next update as I celebrate the culmination of my journey into the realm of LSD-25 Auto. Happy growing! 🌿✨

******************************************** Week 13 Late flower (week 7 flower) ******************************************** Light cycle=12/12 Light Power=150w 63% Extractor controller settings (during lights on). High temp= 25c Temp step=0c High Rh= 50% Rh step=0% Speed max=10 Speed min=3 Extractor controller settings (during lights off). High temp= 21c Temp step=0c High Rh= 55% Rh step=0% Speed max=10 Speed min=3 Smart controller settings (during lights on). Lights on=9.00am Radiator on= -22.c Radiator off=+22.5c Top fan on= Smart controller settings (during lights off). Lights off=9.00pm Radiator on=-19.0c Radiator off=+19.5c Top fan on VPD aim=1.0-1.4 DLI aim=35-40 EC aim=1.0-1.6 PH aim=6.0-6.5 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 NPK= 8-17-26 Method= Automatic Feed=Flower nutes Neutralise=0.1ml/L Plagron bloom=4ml/L Plagron Power buds=1ml/L Green Sensation=1ml/L Easy Ph down=0ml/L (1ml=24 drops, 1 drop=0.04ml) Easy Ph Up=0.02ml/L (1ml=24 drops, each drop is 0.04ml) Ec=1.55 PH=6.2/6.1 Runs=18 Run times=4mins (0.3L each) Gap times=16 mins Total runtime=72mins(5.5L each) Total flowrate= ml/min(ml/min each) Auto start time=10.00am Auto stop time=15.44pm 💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧💧 ******************************************** ******************************************** 📅29/12/24 Sunday (Day 85)(flower day 45) 📋Light power now 150w 63% H=93cm D=22cm Dli=43.5 ppfd=1010 💧 Automatic Water (Neutralise) Easy Ph down= 0.01ml/L ( 3 drop total) Ec=0.15 PH=6.3/6.2 Volume=13L Volume left=2L Volume used=11L(ml/min) Volume each=5.5L(ml/min) Runoff. Total runoff=2.4L Ec=3.5 PH=/6.2 💧 📅30/12/24 Monday (Day 86)(flower day 46) 📋 📅31/12/24 Tuesday (Day 87)(flower day 47) 📋New year's eve. 📅1/1/25 Wednesday (Day 88)(flower day 48) 📋New year's day. 📅2/1/25 Thursday (Day 89)(flower day 49) 📋 💧 Automatic late bloom nutes Ph up=0.24ml, 6 drops Ec=1.55 PH=6.2/6.0 Volume=12L Volume left=1L Volume used=11L(152ml/min) Volume each=5.5L(76ml/min) Runoff. Total runoff=1.1L Ec=3.3 PH=/6.2 💧 📅3/1/25 Friday (Day 90)(flower day 50) 📋 Started snowing 📅4/1/25 Saturday (Day 91)(flower day 51) 📋H=93cm D=22cm Dli=43.5 ppfd=1010 ******************************************** Weekly roundup. 📋 Loving this plant, she's got some decent size flowers. She's coming along nicely. Radiator is still doing a fair amount of work using 28.77Kw £7.19 Back soon. Take it easy. ********************************************

Hallo Growfreunde, da bin ich wieder mit einem kleinen Update 🤗😁. Heute ist Tag 5 nach der Umstellung auf 12/12. Der stretch sollte sich bald bemerkbar machen, aber sollte keine Probleme geben, Platz ist da😎💪. Ich habe diese Woche den Tank das erste Mal nachgefüllt, allerdings diesmal mit nur 20L, damit das Wasser nicht so lange steht. Es hatte sich ein kleines CalMag problem angedeutet, was sich aber schonwieder eingependelt hat. Entlaubt habe ich auch. Große Fächerblätter wurden entfernt, aber sehen tut man dass nach 2 Tagen schon nicht mehr😅😅. Ende Woche 2 werde ich nochmal lollipoppen und entlauben, danach wird nix mehr gemacht. Mal sehen ob der Dünger bis zum Ende reicht😅. Hab heute, 13.10 nochmal die Positionen der Töpfe verändert und die Pflanzen auch anders angeordnet. Nutze jetzt fast die vollen 2m breite😁😁 mal sehen ob ich das ganze Zelt voll kriege🤣🤣😂😂. Was schätzt ihr so was an ertrag raus kommt?! Bin echt gespannt🤗🤗🤗🤗🤪. In 2-3 Tagen mach ich nochmal ein Update, bis dahin liebe Growfreunde 💞💞💞

Legend Timestamp: 📅 EC - pH: ⚗️ Temp - Hum: 🌡️ Water: 🌊 Food: 🍗 pH Correction: 💧 Actions: 💼 Thoughts: 🧠 Events: 🚀 Media: 🎬 D: DAY, G: GERMINATION, V: VEGETATIVE, B: BLOOMING, R: RIPENING, D: DRYING, C: CURING ________________________________ 📅 D77/B15 - 31/01/24 ⚗️ EC: 1.2 pH: 6 🌡️ T: 19-22 °C H: 40-60% (Min-Max values taken from the app.) 🌊 2L 🍗 CalMag - Bloom A-B - Bud Candy - Big Bud - B52 💧 💼 Thanks to the TM+ Pro app and the use of the Humidifier now the T-H of the enviroment is correct and i got quite good VPD (Vapor Pressure Deficit) rate 🧠 🚀 🎬 Added Timelapse video ________________________________ 📅 D78/B16 - 01/02/24 ⚗️ EC: 1 pH: 5.9 🌡️ T: 19-25 °C H: 45-70 % 🌊 2L 🍗 💧 💼 🧠 Quite time to change res 🚀 🎬 Added Timelapse video, 3 pics and "TM+ Pro" App screenshots of T-H values daily graph, and quite perfect VPD daily rate ________________________________ 📅 D79/B17 - 02/02/24 ⚗️ EC: 1 pH: 6.2 🌡️ T: 19-25 °C H: 40-60% 🌊 🍗 💧 Added few drops of pH- 💼 🧠 The res was for many time on the upper side of pH range, so now I would like it to goes down to a more acidic solution. I'll do it on the next res change, maybe tomorrow 🚀 🎬 Added Timelapse video but today it was trunked because of a problem on the camera, but I posted it anyway ________________________________ 📅 D80/B18 - 03/02/24 ⚗️ EC: 0.15 pH: 6.6 🌡️ T: 19-25 °C H: 40-60% 🌊 RES Changed 💦💦💦. Flushing until tomorrow 🍗 💧 💼 🧠 🚀 🎬 Added Timelapse video and VPD, T-H graphs screenshots ________________________________ 📅 D81/B19 - 04/02/24 ⚗️ EC: 1 pH: 5.7 🌡️ T: 19-25 °C H: 45-65 % 🌊 15L 🍗 CalMag - Grow A-B - Bud candy - B52 - Big Bud 💧 💼 Some defolation and sistemation of buds 🧠 🚀 🎬 Added Timelapse video and VPD, T-H graphs screenshots ________________________________ 📅 D82/B20 - 05/02/24 ⚗️ EC: 1 pH: 5.4 🌡️ T: 20-25 °C H: 52-84 % 🌊 🍗 💧 💼 Little defolation and raised up the lamp 🧠 🚀 I forgot the humidifier on manual mode, so H increased until 80%and VPD went out of control 🎬 Added 4 pics, one is from timelapse set. Added Timelapse video and screenshots ________________________________ 📅 D83/B21 - 06/02/24 ⚗️ EC: 1 pH: 5.0 🌡️ T: 19-25 °C H: 45-70% 🌊 🍗 💧 💼 🧠 pH decreased a lot and it's a good thing it stay on the acidic side for a while 🚀 🎬 Added Tmelapse video and screenshots of VPD and T-H

Hello happy friendly growers, All was fine this week, I've started bloom fertilizer and plagron sugar royal this sunday. She look nice and buds are biggest each day Have a nice week :D

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Grandaddy Purple- 31 inches tall Colombian Gold- 35 inches tall Alcapulco Gold- 28 inches tall

2025 Fastbuds GreenHouseGrow has come to an end. Its been an absolute roller coaster of a grow. So cool to watch them grow from sprouts to absolute giants around 8ft tall. They're a great and easy strain to grow and I could only imagine having them in bigger pots how much bigger they could get. I ended up harvesting them for fresh frozen and immediately put them into the deep freezer after trimming everything that didn't have trichs on it. Excited to do some bubble wash and make FreshFrozen Rosin/Hash. All in all highly recommended and Happy Growing.

Not much to do with Autos. I take a handful of leaves everytime I'm in the tent. Veg thru 2-3 weeks of flower.