The discoloured images are zoomed in iomages through a magnifying glass. The main plants are very close to harvest =] I will begin flushing I think after 1 bout of Cannazym, with aim to harvest ~10 days after. I will tailor each to the plant's unique stage of life, as they're not all equal EDIT: Thank you to one of my commentors; Ezzjaygrows answered grow question 6 days ago Yeah I see no point in going to 10.. your diary says these are autos tho? They can take more than 12 hours of light. You got multiple strains going or what? I do have multiple strains, but they're all auto. Thank you for the point added to the list of things learned. I knew they could take more than 12h, but I was managing remotely, and I thought anything but 12-12 would require , say, 18h for 6 weeks, 16 for 2, 12hr for flush period (I'm aware had I given them more light it wouldnt have taken 12-13 weeks.) On the positive side, I saved a picture of a "best case" Creme Mandarine bud, and reasoned my way to "Yeah mine will probably be 35% as nice, in quality, yield etc." - Sure, these arent Cup winners, but FUCK ME, did they turn out so much better than i expected. Now why's that?; I humbled myself and lurked Creme Mandarine grows and learned and learned and learned. So thank you 'Ezzjaygrows', and thank you Growdiaries =] Don't worry. My 2nd will be diarie'd too, and this time, I wont be remote managing with literal remote CCTV and PH meters/Moisture meters in the CCTV shot. I lost 2 seeds thanks to the person i delegated minor observational management too, a young plant was let to dry out, harming it later on it life, AND he second guessed me constantly. Tera Vega / PK was missed because he thought he knew better.. Which he does... For outdoor.

The journey is coming to an end with these ladies, both smelling crazy good and very frosty 🙌 The tropical candy smell on plant nº2 is truly something else and very strong too🍭🍭

Gracias al equipo de Terpyz, Marshydro y XpertNutrients sin ellos esto no sería posible. 💐🍁 Variegatd Sour GPP: Es una cepa que mezcla Sour Diesel, Spicy Temptation (GPP) y la línea Variegatd, conocida por sus hojas visualmente impactantes. Se espera que combine el perfil ácido y terroso de Sour Diesel con un toque especiado de Spicy Temptation, mientras que la influencia de Variegatd añadirá colores y patrones únicos en las hojas. Ideal para cultivadores experimentados, esta cepa promete una experiencia visual fascinante y una mezcla compleja de aromas y efectos. Estoy usando el FC6500 como luz principal 💡, con su potencia y espectro, espero un crecimiento fuerte y buena floración 🌱✨, también usaré los Adlite UV55 de 55W para complementar las primeras etapas. En cuanto a nutrientes, he optado por XpertNutrients 😎, que es conocido por su fórmula equilibrada y fácil de usar. Sus productos están diseñados para ofrecer una nutrición completa durante todo el ciclo de cultivo, ayudando a las plantas a alcanzar su máximo potencial. 📆 Semana 5: Esta semana la Variegated Sour GPP avanza con fuerza en floración. La estructura de la planta es sólida, con cogollos bien formados que empiezan a cubrirse de una generosa capa de resina. Los Adlite de Mars Hydro están haciendo un gran trabajo, asegurando una distribución de luz excelente que favorece el desarrollo parejo de todas las flores, incluso en las zonas más bajas. Se nota la diferencia en la densidad y calidad de los cogollos. El plan de nutrición de XpertNutrients sigue funcionando a la perfección: hojas sanas, crecimiento equilibrado y una floración limpia, sin síntomas de carencias ni excesos. Todo apunta a una gran cosecha si seguimos así. Seguimos creciendo fuerte 💪.

Day 36 of flower and looking great all in all. I’ve been watering her every 2-3 days and watering her about 1.5 gallons of water per watering and alternating between full feeding schedule and cal mag only watering. She’s showing some relatively minor calmag deficiencies on some of the oldest leaves but nothing too bad. I’m guessing she’s needed just a little bit more calmag than I’ve been giving her. I feed with calmag every feeding and give 5 ml per gallon. I’d say around a month or so left although the pistils, calyxes abs trichomes will show me when she’s ready. The smell is one of primarily a citrusy smell mixed with undertones of gas/diesel. Maybe 80% citrus and 20% gas/diesel.

Halfway through week 7. Couple pictures from Tuesday. Gonna upload more when I get back home to her. Can’t raise the lights anymore. Hoping she is done stretching. She takes up every bit of that 4x5 box. Couldn’t be happier with this grow so far. I think next time I’ll go down to 5 gal smart pots this way I can pump out two of them. Still in disbelief she has grown this fast and started budding that fast as well!! I’m feeding her 2 gallons every 2 or so days. She is a very thirsty girl and let’s me know when she is hungry too (leaves curl down). I’ve been slowly defoliating the bottom of her. I cut a couple under grown budds from underneath as well. Not sure how she’d respond if I went scissor happy. I also can’t say enough about Advanced Nutrients. Since I made the switch I can tell her leaves are always praying up and overall health seems through the roof! She really is a monster. 31 inches wide by 36 inches tall. She is stacking nicely. Did my best with pictures. Can’t tell if the flash on the phone helps or hurts the pictures. End of the week- She is doing awesome. Blowing up pretty fast and there is a serious citrus pine smell coming off her when you bump into a budd or it wipes on your finger. For the most part it seems as if she is loving life!!

Week to of flower ad she already smells like a bouquet of flowers...Perfect time to pollinate her with the male skunk polle

d33 Hoy subo fotos unos días antes de esta semana, mejor, tendremos más fotos. Hoy me he puesto a revisar un poco las plantas y me he dado cuenta de que las Lemon Krystal son hermafroditas, la que estaba en la parte de dentro, solo tiene unos pocos sacos de polen que todavía no están maduros, se los he arrancado y cortado 2 ramas que tenían mala pinta con eso, el resto de ramas parece estar bastante bien y son flores macho, la Lemon Krystal que esta mas cerca de la puerta del armario, esta bastante mal y la tendré que desechar ya que no quiero que polinice al resto de plantas. También una Kandy Kush, la de la derecha, le he quitado 2 flores macho, no parecía tener más, unas flores espontáneas, pero semillas a granel a veces pueden pasar estas cosas, no es lo normal, pero parece que la cepa de Lemon Krystal esta un poco jodida... Para añadir, la LK que parece que podremos sacar adelante(pero bajo supervisión) tiene una rama que tiene 4 cogollos en vez de 2, mola, pero si no se pone a sacar flores macho, la podremos continuar haciendo seguimiento. Tenemos un cogollo de la Kandy Kush(planta del centro) que es doble. Puede ser que tengamos algo polinizado en el Indoor, pero pues es algo que ha pasado, espero no tener muchas semillas mermara la calidad... d35 Unas fotitos rapidas... Parece que estan progresando las deficiencias, a final de semana añadiremos un poco m'as de abono a ver como responde

Day 39 from seed!! I’m exited to watch the explosive grow of this genetics soon i will be take some clones or each pheno to take the best and put in the next batch!!! Stay tuned

i killed the poor girl ... i had too many infrastructure issues in the form of leaks and nozzles falling off. we had a funeral, then i tore the house down and rebuilt it so we not have the same none girl related issues again. i also started another Hawaiian Thai kush seed from my rare collections of zilizopendwa -. the loved nuggets

3/28/25 chopped and humidity was a little high starting off 3/30/25 have them drying at 60% and 70° 4/3/25 Still needing a day or so of drying to be at a stable humidity Beautiful plant. Fan leaves with Triched out patterns visibly everywhere. Even though i chopped quite a bit off of the plant, i still got quite a bit bigger yield than i expected. All sizable nugs. Pulled 65 grams.

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ DAY 147- DAY 98 Flowering ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Welcome to a new week :D I'm starting to reduce the nutes. I am thinking about 1-2 week till harvest. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ DAY 149- DAY 100 Flowering ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

8/15 Yesterday the girls finally got some sun. They're flowering. Like crazy. I do need to do an application of BT once this rain stops. It just started up again. Went back over and things were dry. I decided to hold off watering until tomorrow when I feed. 8/16 Watered the 50 2 gallons, 10 1 gallon, and the rest about 1.5 gallons. I fed a pint to the ten, 2.5 pints to the 30 and 2.5 pints to the FIMMed bluecheese that looked hungry. This way I can tell if they can handle larger doses. I'd like to keep it as small and simple as possible. Gave me a fright this morning when a sheriff pulled in while I was feeding. He was on his way to a neighbors but it looked like he was just admiring things lol. I'm all legals there's no problem there. I put off spraying BT last night because dad had a couple ppl drinking and I didnt want to interact with them. Plus it was to early. The sun hadn't crested the barn yet. At least now u know what time to go spray. I'm finding some lady bugs and I killed my FIRST aphid of the season. I still have caterpillar holes in some leaves though so I might as well hit them with the BT (even though I actively hunt them). No one ever said growing cannabis is easy. Oh and my soil must be BANGING because I found some mushrooms growing in it and like a mycelium web which is awesome. 8/17 This was a quick morning due to an upcoming doctor's appointment. Removed some grass that had grown on the tar from spilt soil as it had diseases on it. I don't want that spreading to my plants. I have one blueberry cheese that I FIMed with four cola's that doesn't have as many leaves and has dropped a few leaves but looking at ot from the other side it doesnt look as open. I know that these girls are transitioning and this is the time I lose leaves. However some plants still seem totally green with no problems despite flowering. I'm wondering if it's a specific phenome thing. I also noticed that the caterpillars are still there and this morning would've been a great time to apply BT but I didn't have time and since I upped the feed I didnt want to do two things at once and have a problem and not know what it is. 8/18 Rained overnight. Plants were soaked. It's not raining now. Plants are definitely overwatered. I'm losing a few more leaves that are yellowing on the bottom interior of a couple plants. Some of it could be from the transition to flower ir the large amount of rain we've been getting. At least my drainage is really good. I need to pull the fence out and co soder cutting a pallet I. Half to better utilize space. I know I say this stuff all the time and I'm not sure it NEEDS to be done. What DOES need to be done sooner rather than later is to apply BT. I can see the damage from them. It's not bad but I want to avoid botrytis as much as possible so those pillars have got to go. Otherwise I've never had plants this healthy and it's been a real ahotty season. Boy those 10th planet's are all mice and green and healthy looking. I'm really looking forward to flowering these strains and see what they can do. I'm already greatly impressed with what I've experienced so far. UPDATE: Wasn't raining earlier then it Rained off and on but just a light steady rain that plants love but it's 3:30 now and it's pouring. Guess I won't be applying BT tonight. We need some sunshine but unfortunately I can't control the weather. Just sitting here thinking I was wondering about using Plant Doctor preventatively just due to the steady rain but I noticed mushrooms growing in my soil and since I haven't not9ced any type of fungal problem I'll revisit that idea another time. Polycarbonate roof or even some form clear plastic and a rough frame of pvc or extra lumber. Theoretically I believe I could move them all indoors for a little bit if need be but that would he a huge pain in the ass. Honestly I've got great drainage and my watering seems pretty good so I might be alright doing nothing. We shall see knock on wood. 8/19 Plants were soaked this A.M. I took a video and uploaded it (which takes forever). It's sunny though today and after the sun comes out after a period of rain the plants grow like crazy. Everything is flowering nicely. I think I can go to the end of October. I talked to a lady today that said she'd already harvested some of her peanut butter breath clones. It's my buddies mom and if you look back at one of my diaries NY mendo breath was done in September. These girls have a ways to go but the are explosively flowering which is good. It's easy to tell which strain is which. Pheno's vary but the flowers on the plants are easy to distinguish from one another. I've also never had plants stay this healthy green this long! Previously I'd have multiple pests, wpm and maybe septoria at this point. I think I've upped my game. Knock on wood. My worst problem now is worrying when to spray BT for the miner caterpillar problem. I have wasps, lady bugs and a shit ton of birds from the 3 bird feeders. I see them dart in and out of my cage. Chances are I'll spray BT tonight and take some pictures if it's nice out. 8/20 The sun is finally out. Bags are still heavy. Looks like it Rained again. I found caterpillar shit so I REALLY need to get that BT applied. I also saw minor damage to a budsite on a low hanging branch. Looks like those fucking earwigs bur I'm probably being paranoid. I also had to remove the rope that I was using to support "the big cheese" because with all this wind and rain it "rubbed" against the stem and caused an injury. I don't need that now anyway with the trellis up. Bids are developing rapidly and stacking up nicely. I have a couple plants that aren't as green as the others bit it may be do to all the rain we've been getting. I think I need to increase the volume of liquid i give my plants when I feed. Next feed I'll be doing at LEAST a quart. Due to my w8fes illness I haven't been able to spend as much time there as I should. No fungal stuff "YET". I have seen some leaves that show the plants have been over watered. Drainage is good though. I forgot to upload the video from this morning and it's uploading super slow so I'm gonna have to upload it tomorrow 8/21 Awesome. Went to town and left without going to my doctors appointment. Good job man. 👏 ANYWAY....My phone was dead when I went to town so I coukd only upload one video. I'll have to do the others later. I mixed up a batch of bt 1 tablespoon with half gallon of water (as that's what this hand mister holds. I went around the cage and sprayed every angle. Then I went to each individual plant and sprayed while spreaking the branches to make sure I got the interior. I used almost the entire half gallon so I'm hoping this will help. Bags were heavy but doesn't take them long to dry out. I'm wondering if the yellowing that is moving up (on the small purple punch and the one in the tote) is because they need more nutes, they're transitioning to flower and really stacking up or because they've been over watered with all this rain. Opinions are more than welcome. I'll check the girls tonight and if the are wet enough I'll feed and at a higher volume. If they aren't ill wait till they get their full water. MY WIFE WAS HAVING AN "MEDICAL PROBLEM AND SO ON THE WAY TO THE DOCTORS I CHECKED THE PLANTS. THE 10TH PKAMETS WRRE DROOPING. I QUICKLY MIXED UP ENOUGH TO FEED EVERYTHING AT LEAST A GALLON AND THEN GAVE THE ONES THAT WERE THE WORST LIKE AN EXTRA HALF GALLON. JUST ENOUGH TILL WE GET OUT OF THE ER THEN I'LL GIVE THEM THE OTHER HALF OF WATER AND ILL FEED THEM. AT LEAST MY WIFE IS OK. UPDATE: Took my wife to the hospital. They did a shit ton of nothing which is par for the course. However, luckily for me (yeah right) I got back early enough to give the plants another half gallon of water each and then I fed increasing the volume to 2.5 pints (upping the big bloom to 3 tsps per gallon) but I ran out of feed on the last plant. I only mixed up two gallons and it was super dark so thar one container plant will need to be fed tomorrow I guess. This is masterofsmeagal signing off to fucking FINALLY get stoned!

A new plant was born, thanks to the seeds of Sweet Seeds, I hope the best girl will grow up

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

All plants are happy this is day 1 flowering stage (13/5/20). Looking forward for the flowers.. Two lights 1000w LED turned on All lights 12 inch from top of the plants Light schedule switch 12/12

Hey everyone 😃. Both phenotypes are beautiful, the Pehno 1 is of course the more beautiful due to its red color 😍. Both smell really delicious 😃. Now to the weekly overview. Days of flowering 42 Since the water was only poured on day 41, both were only checked for their health today. Flowering day 43 Today both were poured with 1 l each, completely without additives as they should use up their remaining nutrients :-). Flowering day 44 The tent was cleaned and the humidifier refilled. Flowering day 45 Today both were checked for health and sprayed. Flowering day 46 Today everything in the tent was tested for functionality (fans, etc.). The humidifier has been refilled. Flowering day 47 Again today it was poured with 1.2 l each time, and the tent was cleaned. Flowering day 48 Both plants were checked to see how wide their trichomes were (70% milky, 20% clear and 10% amber). The plants are really extremely fast 👍. Everything was cleaned again and the humidifier was filled. That was it pushed with this week, and I have to say the Fast Version lives up to its name 😳😃. I wish you all a lot of fun updating these beautiful phenotypes, stay healthy and let it grow 🙏🏻🍀 You can buy this Strain at : https://sweetseeds.es/de/red-mandarine-f1-fast-version/ Type: Red Mandarine F1 Fast Version ☝️🏼 Genetics: Red Poison Auto®️ (SWS39) X Tangie (California Orange x Hybrid Skunk) 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

New clones just arrived! Starting them off straight into coco coir this time. First transplant today into solo cups. They got a pretty heavy first watering with a PPM of about 425. Hoping to really start them off strong. I will be vegging them for much longer than usual. I typically veg my plants for 4 to 6 weeks and these girls will be vegging for 8 to 9. This grow will be a great chance for me to try some HEAVY plant training techniques.