2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Hey Freunde heute ein kleines Update, mittlerweile ist Blütetag 24 der stretch war wohl noch nicht ganz zu Ende und besonders die Papaya und die Pink Chai haben nochmal nen richtigen Schub gemacht. Heute hab ich mal doppelt soviel Wasser gegossen, da die unteren Blätter schon leicht vertrocknet waren und die Töpfe immer sehr leicht. Die Pink Chai bekommt minimal gelbe spitzen, aber is glaub zu vernachlässigen, verdacht war zuviel Licht. Sie hat danach auf jedenfall auf gehört die Blätter zum Licht zu strecken, also erstmal keine praying leaves mehr, heute Abstand verringert und sie Betet wieder. Freu mich über jede Rückmeldung und wünsch euch n schönes Wochenende! ✌️💚

Inizia a esplodere di gemme non sono molto grandi ho sbagliato in passato e la piccola ne ha risentito. Ora però si e ripresa e piano piano si sta facendo valere. manca ancora un po' alla fine del ciclo non vedo l'ora di fumarla tutta ! Dajeforte growes

Plants are nearing the finish line. Might not all be quite ready in the next week, but I likely will have to chop them all. I removed some leaves from the center plant (A) and the overfed dark green leaves become obvious.

Seedsman Seeds Germination Report - Run 01: The White OG Journey Kickoff to Germination: The Start of Something Great! Welcome to the beginning of this adventure with The White OG feminized seeds from Seedsman Seeds. Excitement is in the air as we’re searching for strong, resilient plants, and this journey has all the best tools and tips. Starting with five seeds, we’re aiming for a solid beginning and maximum potential. Let’s explore every step in detail to make this grow as robust as it can be! Germination Process: Tools of the Trade TheCannaKan: The Ultimate Germination Gadget I’m thrilled to share my love for TheCannaKan, a little germination wonder! This tool is essential for any grower who wants to start their seeds with precision and care. I noticed rapid results in just 24 hours, with the seeds showing signs of life. At about 32 hours, I transferred them to the Root Riots, which brings us to the next exciting stage. Why I Love TheCannaKan: • Quick Results: Seeds sprouted rapidly, minimizing wait time. • Consistency: Keeps moisture levels optimal for uniform germination. • User-Friendly: Simple yet effective for both new and experienced growers. Root Riots and Their Role in Root Development After sprouting, I placed the seedlings into Root Riot cubes under a dome. Root Riots are fantastic for stabilizing young plants, providing an airy and nutrient-friendly environment that promotes robust root growth from the start. Benefits of Root Riots: • Root Aeration: Soft yet structured, these cubes support root expansion. • Moisture Retention: Holds just the right amount of water for growing seedlings. • Enhanced Root Health: The cubes are ideal for preventing damping-off and ensuring healthy, white roots. Nutrients to Kickstart Growth: Aptus Holland Regulator and Startbooster To give the seedlings a perfect start, I added Aptus Holland’s Regulator and Startbooster (1ml/L each). This mix promotes early root and stem strength, giving seedlings the ability to absorb light and nutrients more efficiently. • Aptus Regulator: Enhances plant resilience, aids in nutrient absorption, and promotes early vitality. • Aptus Startbooster: Adds a balanced mix of growth hormones and nutrients that are ideal for the early stages, encouraging healthy roots and faster seedling growth. Light and Environment: Future of Grow FOG LED with TrolMaster Tent X Controller I’m using a Future of Grow (FOG) LED at 600W, but keeping it dimmed down to around 200 PPFD. This setting is controlled by the TrolMaster Tent X Controller to give the plants the perfect start without overwhelming them with intense light. Controlled lighting helps seedlings to develop without stress, encouraging healthy growth and internode spacing. Shoutouts: • Future of Grow LED: High-quality, efficient LED light that adapts well to seedling and veg stages. • TrolMaster Tent X Controller: Reliable and intuitive control over light, humidity, and more. Perfect for a hands-off, efficient environment setup. Observations: One Seedling Takes the Lead At the end of this germination stage, it looks like one seedling has emerged as the strongest, while the others may need a few more days. Patience and observation are key; some seeds naturally take a bit more time to sprout. I’ll keep monitoring these with hope, allowing each seedling its own rhythm. Grower’s Notes and Tips • Consistency Matters: Germination is all about steady conditions—consistent moisture, gentle nutrients, and stable lighting make all the difference. • Document Everything: Keeping a log of each step (like this one!) helps track growth patterns and provides valuable insights for future grows. • Stay Positive and Patient: Not every seed will sprout at the same rate. Trust the process and remember each plant is unique. Community Shoutouts Thank you to everyone who makes this process smoother and inspiring—TrolMaster, Aptus Holland, Future of Grow, and Seedsman Seeds for amazing genetics and tools, as well as the growers’ community who share insights, tips, and encouragement along the way. Next Steps Now that we’re through germination, we’ll move into the early growth phase. I’ll keep documenting the lighting adjustments, nutrient changes, and growth patterns of each plant, especially that eager seedling leading the way! Growers Love, and here’s to a strong start for the future of this grow! 🌱 DISCOUNT CODE - SeedsmanSeeds - DOGDOCTOR 10% off As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and so joyful with you all in my life 🙏
 With true love comes happiness 💚🙏 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so 💚

 Friendly reminder all you see here is pure research and for educational purposes only 💚Growers Love To you All and remember to keep that smile big and alive 💚

This week was amazing! I gave green sensation for the first time, and the results are as good as I expected. The buds are incredible, the smell is amazing, everyone looks healthy so far! :) I hope everything will be alright, see you guys next week!

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23.10 - 5L osmosis water 24.10 - 5L osmosis water , no Pic 25.10 - 5L osmosis water , no Pic 26.10 - 5L osmosis water 27.10 - 5L osmosis water 28.10 - 5L osmosis water 29.10 - 5L osmosis water

At the end of Week 3 from Seed, this is how the ladies are doing. Could be doing alot better but they have been transplanted and topped so I will give them a chance to recover fully. Happy Growing guys, any questions just ask 👍🏾🌱💚

Started having some leaf issues at the end of week 6, defoliated, gave medium/heavy feeding of big bloom. Going to start flushing with distilled water for a few days and see if any improvement, only fan leaves affected at this time. Day 45: checked ph of soil, showing 7.0, just ordered some fox farms ph down should be here in 48hrs Day 49: bought a reliable pH testing pen and found my distilled water to be 9.5! Mixed up some spring water/nutes/pH down at 5.8/5.9 . Hopefully this does the trick!

This this so strong smell !!

We back with some buckets! Time to fire up the pumps and get this show on the road. Soaked this beautiful little gem of a bean in some water until it cracked then threw it into the bucket with 100% perlite. Started them on a low dose of maxigro on day 3. Doing these diy Dutch Bucket style as always. Going to be running 8 in house genetics strains on the system with constant 24 hour a day irrigation. Not much exciting going on yet, but with a little luck that may change pretty soon. A huge thanks to @wildeweed for this little truffle.

Really healthy leaves, however salt buildup starting to become evident on the roots, been giving occasional flushes with tap water that still has chlorine, and 40ml of h202 then the next day giving the next weeks bucket with 6mL of hydrogaurd and 3/4tsp of Great White (Every bucket) Have to change bucket every 2-3 days, at 3 days it’s damn near empty.

Day 63 - lookin good.... haha.... um, tanks stabilized, ph 5.8 - ppm 1200 .... she's feedin alot..... buds are fillin and lotsa white furries.... listed the nutes and amounts etc.., but i'm kinda feeding her as she needs.... so every few days she is drinking 2 lts +- and i'm just mixing up a neut solution in a 2 lt bottle every day or 2 ..... sometimes she just wants abit of water...... Day 64 +vid....shows her deep green and gloss... abit of crystalic :) Day 65 - vids pics.... trimmed her, and abit of bending.... Added blood and bone.... she's stretching alot, 60cm+ ........ Day 67 took all the early buds on all the stringy 0ff shoots.... Poor lighting vids, but don't want to mess with her flowering, :) 👍😎👊

It's Day 35 Week 05 Of Flower For My 02 Kombucha Cream By atlasseed . And For My Snow White and SpliffStrawberry By Spliff Seeds Amsterdam . Today was Hair Cut Day For All 4 Lady's. Happy Growing Growmies 🤘🏻

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Thats what i mean, she will need some more time then my other. I think its will be a 12 weeks harvest. But she will be fat too

Started to defoliate and lollipop her slowly, cut back on nitrogen completely and will only be using a tiny amount of Micro and Big Buds nutrients for the remainder of the grow.

Just sitting here watching the frost and weight pack on. All of the top leaves are starting to die as I back off on nutrients. I'm going to finish them for one more week with water and floral kleen. I'm really pleased with the appearance of these plants using Oregon's Only Nectar for the Gods Basic lineup.