June 16, 2024 Day 57 This week nothing changes with the nutrient schedule. I cleaned out the reservoirs and gave fresh nutes. I also made sure to double check the height of all the plants and we have a final height for each of them. Sour Diesel A is looking pretty good. Her final height is 23". Her bud production is looking pretty good as well. Trichomes are coming through quite nicely. Sour Diesel B is looking really good with her frosty fat nugs. Her final height is 21". I haven't touched her so I don't know how sticky she is nothing. But I see good things coming to this lady. Huge buds and lots of frost. Sour Diesel C is frosty, covered in nugs and has a final height of 30.5" tall. She looks fantastic and I wonder what her final yield will be. Her bud production is coming along nicely with buds starting to swell. Sour Diesel D is shaping out to be my lowest yielder. She has very little bud on her and what she does have is a bit slow to develop. I have a feeling this will be my extraction plant. Edibles and concentrate. The lighting is looking great at 45 mol/m²/d. Nothing to change. The plants are very happy. The environment is almost back to normal. The humidity is below 60% for the most part. Yesterday it was all the way down to 52%. Today just under 60% for the morning anyway. The Central Air should be kicking on in an hour or two. Grow System Environment: Temp: 74.6° RH: 55.3% VPD: 1.27 kPa June 17, 2024 Nothing going on today. No watering, no training, no pics. Just a smooth day in the tent. Sour Diesel A looks good. Lots more frost. Buds are swelling. Everything looks great. Sour Diesel B had a couple leaves popped off, ones that were overlapping Sour Diesel C. Her buds are huge and I'm already starting to see orange pistils. She smells awesome too. Definitely Sour Diesel smell. Sour and gas. And a bit of sweetness as well. Very nice aroma. Sour Diesel C is looking fantastic. Her buds are also swelling and her trichome production is amazing. So frosty and smelly. As long as the buds continue to swell at this rate, this lady will be a pretty good yielder. Sour Diesel D is small as ever. Her buds are swelling quite nicely. They are bigger than A and C for now, but not as frosty. I'm honestly not sure if I did something wrong or if this is how she grew naturally. I didn't screw up the nutrients, nor did I add any stress except for the small defoliation. But that was right when she stopped stretching. I guess I'll find out at the end of the run. I also pulled a couple outer leaves off to keep them from overlapping other plants. Lighting is of course the same as yesterday, and won't be changing until the last 2 weeks of flower. I might drop the intensity down a bit for the last leg of the run. I'm going to research this though. As these are autoflowers they're fine with constant light, but at the end are they looking for less light? I think so. Naturally in the world, the end of a growing season is when it starts to drop on temp and light. So it would make sense to drop the light intensity the last week to help induce maturity and more trichomes. That's what it's supposed to do. I'm not sure if that's the case with autos. Will research and report back. The environment was great yesterday. Today it's a bit humid in my room but it's also kinda humid outside and 83°. It's supposed to climb up to 88° today, so I'm hoping the central AC will kick on early. The room temp is well controlled around 70°. Once the AC kicks on the humidity should drop down to around 52%. Not ideal, but actually within range. If I can somehow get it below 50% I'll be happy. 52% is merely satisfactory but not perfect. Grow System Environment: Temp: 75.7° RH: 56.1% VPD: 1.30 kPa June 18, 2024 It's Picture Day! Did some stuff today. No watering or anything like that. Sour Diesel A is doing quite well. Her buds are swelling more and more. She's even frostier. I cannot wait to see how frosty she gets by the end. I also checked her roots. I figured out why the container is light. The roots are taking in all the water they can directly before it gets to the container. I'll have to top feed in a day or 2 if she gets lighter. However, she hasn't lost anymore weight in days, but I really need her to moisten the soil in the container. Sour Diesel B is now producing orange pistils. And kinda rapidly. I knew she would be done first, maybe a couple weeks left for her. I think next week, if she is as far along as I think she will be, I'll switch out Big Bud for Overdrive for the rest of the grow. But we shall see. Sour Diesel C is looking really good. Her buds are swelling more and she's even more frosty. As we all know, this one is about a week behind the others, so I expect more swelling and some fat buds. Sour Diesel D needed some leaves trimmed today. They were super overcrowded in one particular spot. So I took 3 of those and then a couple outer leaves that grew out since my last defoliation. Her main cola is getting fat. Just not fat enough. I have a feeling I'm getting no more than 2 oz. I'm guessing just over an oz. We shall see. She's definitely not big enough and doesn't have enough bud to produce a good yield. Oh well. The rest are pretty promising, so I'm not too worried. I added to the lighting today. I have supplemental lighting from AC Infinity. So I installed them today just below the top of the canopy. It should help with the lowers and get more fat buds throughout all 4 plants. I'll keep it at level 7 for today and see how things look tomorrow. The only concern I have is too much light from the main light due to the extra light from the supplemental bars. However, it should do quite well. So we are in a humid heat wave for the rest of the week. The Central Air should be kicking on anytime and will definitely drop the humidity down to about 50%. The temp should stay around 75° to 76°. Currently the humidity is really high at 66%. I'll be keeping the tent door open every hour or so until the A/C turns on. The temp is kind of high as well at 79°. I think I need to try to get the bedroom temp down to 76°. Wish me luck. It's already 82° and climbing. Afternoon update: Damn it's hot. Grow System Environment: Temp: 76.7° RH: 55.8% VPD: 1.34 kPa June 19, 2024 Not much going on today. I may need to water, but that's a maybe. Other than that, just the one pic. Sour Diesel A looks great. Surprisingly, she is nearly empty where the other 3 have more water in them. Her bottom is also more moist than normal. She's in the way back and I didn't bother taking the plants out today. I'll take her out tomorrow and inspect her for any issues. Although, I'm pretty sure I'll be refilling her reservoir this evening and might as well refill the rest. Sour Diesel B is getting more orange pistils. I will have to research when I should be adding Overdrive now that I don't think she has more than 3 weeks left. 2 of flower and 1 of flush. Maybe just a week more in flower. I'm not sure. Researching. She's also getting the natural foxtails I read about. Apparently Sour Diesel, in general, foxtails. We shall see how it comes out. There should be no foxtails based on the environment unless the humidity can cause it. I'll have to research that as well. Sour Diesel C actually took the least amount of water for this watering. She's still about half full where the rest are near empty to about a quarter full. I find it interesting that she isn't drinking like a fish. She's in the second half of flower and should be super thirsty. I'll check the moisture content of the soil tomorrow when I refill the reservoir. Sour Diesel D is getting a nice fat main cola. She is the second most empty with less than a quarter gallon left. It's funny. I'm starting to think it's not the plants, but the dehumidifier on that side. I think it's evaporating the water faster than the plants can drink it. I might have to either move it or take it out all together. We shall see. The lights seems to be doing quite well. I don't see any stress from the supplemental light bars. If I start seeing it, I'll drop the power down to 50%, but I doubt it. They are attached to the poles vertically. I would have much rather had them horizontal, but I took my support bars out. I can't reinstall them or I won't be able to take the plants out. So that's out the window. So vertical it is. I may however install the inside bars if I can and then mount the front one to the tent door with a magnet bar. I might try that tomorrow when the plants are out of the tent. The environment is killing me. When the Central Air is off, the humidity spikes like 12%. Now the humidity is ridiculously high yet again at 67%. I really hate not having a wall or window AC. That would solve all of my problems. So I'm screwed until the AC kicks on. Grow System Environment: Temp: 76.1° RH: 59.2% VPD: 1.22 kPa June 20, 2024 It's picture day! We have some happy ladies and there isn't anything to really do today. Sour Diesel A is getting fatter buds. They are kind of small, but it's still only week 4 of flower. She's still a little light. I might top feed a quarter gallon. Maybe with Recharge in it. I'll have to decide what to do. She's definitely not struggling, but she does have upward clawing edges. I'll have to research what that means. She is still the frostiest of the bunch. Sour Diesel B is getting even fatter. Frost is starting to come through pretty quickly now. I can't wait for this one to finish up. Sour Diesel C took quite well to the small bit of top watering I did yesterday. Her buds are still small, but that is to be expected with this one. I can tell they will swell up pretty good and there will be a nice bunch of colas. Sour Diesel D looks great. Her leaves are a lush green still, her buds are getting fat and stacking like mad. The buds are so close together, the plant looks like it has a tiny football on its top. I wonder what she will look like all trimmed up. Probably very small. The lighting seems to be fine now. The praying has lessened, so I know the lighting was an issue. Now that it's fixed, it should be business as usual. The environment is absolutely wrong now. The humidity is in the 70s today. And the temp is in the 80s. Just have to wait for the house AC to kick on. Grow System Environment: Temp: 75.7° RH: 58.2% VPD: 1.23 kPa June 21, 2024 Nothing going on today. No watering, no light changes and I didn't even pull them out. I can see everything that I need to see. Sour Diesel A is getting orange pistils now and seems to be swelling more. Her buds are a little small, but I expect them to swell up over the next 2 to 3 weeks. Sour Diesel B should be done in a couple weeks. She has orange pistils everywhere. I was thinking she would go the same time as everyone else, but it looks like she will be done a week before the rest. Sour Diesel C is looking great as ever. She is a week behind and has all white pistils. Her buds are also a bit small, but I expect them to fill out over the next 4 weeks. Maybe 3. Sour Diesel D needed a few leaves cut from the main cola. The foliage is just way too thick there. I'm afraid the buds won't swell because there's no space at all. Even cutting the leaves off did next to nothing. The lighting didn't change at all. That's it. No changes. The environment is doing the same thing as the last 3 days. High humidity in the morning and lower humidity in the afternoon. Today the temp is a bit wonky sitting kinda high at 78°. Too high for happy trichomes. I'm working on dropping the temp back down to 75°. 74° would be ideal, but this strain also likes it really warm. Grow System Environment: Temp: 75.4° RH: 59.1% VPD: 1.20 kPa June 22, 2024 Last day of week 4 and we got some pictures! And a video. Sour Diesel A is doing quite well. She has some pretty nice stacks going on. But the most trichomes production. I also popped a few leaves off. They were getting crushed. Only a few though. Sour Diesel B may be ready for Overdrive. I'll check tomorrow, but I don't think she'll be growing much more. Her pistils are about 50% orange, but still stark white on the bottoms. So I might wait a week before starting Overdrive. Big Bud is supposed to go to week 6, but this is a 45 day flowering cycle. A total of 6.5 weeks. Based on that info, I think next week will be a good start for Overdrive and then leave it for week 6 and then flush for week 7. And then harvest. I also took 2 leaves off. They were also being crushed. Sour Diesel C is coming along nicely. She has super long stacks covered in frosty bud. Not a single orange pistil. I'd say she has another 3 weeks before flush. Yet another plant that I took some leaves from. Just a few though. Sticking out and overlapping other plants. Sour Diesel D has this hot mess of a main cola. It's ridiculously large, but it's just a whole bunch of super tight nodes. I'm not sure what it's going to do, but trimming it will be a super pain in the bottom parts. No leaf removal today, but I did take 3 yesterday. As for lighting, I think I should have left the support bars in the tent. I'm not really liking the vertical supplemental lighting. I'd much rather them be horizontal along each wall. I feel like that would be the best way for adding the lower light. I may add the bard tomorrow, but just the back and sides. I still need to be able to pull the plants out for watering and to check for any issues. Not that I think there will be any issues, but you never know. The environment is the same as yesterday and the day before. High humidity in the morning and lower humidity in the afternoon. The temp is bouncing around a bit. Up to 80° this morning and now down to 78°. Too high. I need to try to drop the heat down more. Grow System Environment: Temp: 75.7° RH: 59.3% VPD: 1.21 kPa

The weight of these buds is unknown, but the Masters of this site will be visually approximate. I have all the confidence in their knowledge in elementary physics.

Well pretty good week I would say, the only issue is we dint really see the total amount of our stuff but it was still great.

terminando la 2° semana y empezando a la 3° cumpliendose 21 dias pasamos a macetas de 1lt a 7lt y contiamos el crecimiento de estas cepas de rayalqueenseeds y utilizando un sistema de aumento de c02 abase de micelio , las plantas respondieron increible las raices EXPLOTARON ! tanto que colonizaron la maceta y la red baja y tuve que romper las macetas para salvar sus raices, increible crecimiento, - abonamos oganicamente y complementamos un mejor suelo mas vivo y el trasplante salio un exito ! y los resultados de el.. se ve en las fotos !

No problems while drying. Really strong smell while drying you should use a filter-system for that.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Will grow again for sure shout out to @420fastbuds for the strain what a all round great plant!!!!

Ended up with around three ounces of sweet goodness. Strong lady.

She looks fantastic,she's in a 7l pot and she has the same soil as her sister gorilla girl and cream mandarine and darkr devils all planted the same day and she's the biggest one on a 7 l pot. This is pretty strange,anyways, she looks super happy, full 100% organic grown well see howbshe ends up performing

Beautiful growth overall, the tallest plant stands at around 54.5 inches with a potent Kush Aroma. Another week but no dramatic changes aside from a slight red color developing on the leaves.

It's doing it's normal tall Jack Herer growing. Definitely have to start training soon, she'll be going outside but I want to keep her shorter almost bush like. We'll see how that goes with a tall girl?!

hi to all, masters and beginners! welcome to a new week of cultivation with the peaky queen let's cook these flowers a little more to make sure they have more powerful effects and to make the lower part develop well stay up to date!! we will begin by removing some of the excess nutrients in the soil and then giving only water on the next round 😎👽

This is honestly one of my favorite strains I have grown so far, it is amazing all the way around! Smell is like a funky ice cream, taste very fruity & very strong! Will have some more updates and a weight update soon, thanks for following friends!

🌿 Week 4 — Bushy & Thriving! 🌿 #PermanentMarker #ZamnesiaSeeds The girls really filled out this week — super bushy and thick growth all around! I topped them once and gave them a good defoliation to open things up, as they were getting quite dense. I bumped the feed up slightly to 600 ppm @ pH 6.0, still running the Aptus Clean Program with R.O. Water Conditioner, Regulator, and All-in-One Liquid. 💧 Raised the Future of Grow Black Series 600 a bit to encourage some stretch before the flip. I’ll be adding an under canopy light (UCL) as soon as it arrives, and later this week I’ll set up a trellis/net for support. The plan is to flip them after week 5. So far, they’re looking really nice and healthy — one pheno is even showing a bit of color on the tops already. Excited to see how they develop from here! 🚀

Topped this girl this week, she’s growing pretty good. Very nice looking plant to my eye.

🚨 Northern lights week 10 update 🚨 🚨 week 7 of flower!! 70 days old!! 🚨 Hey fellow growers!! This is my last post of this Northern lights before chop!! She is being flushed in prep 4 harvest!! She has matured nicely with every moment given under her beautiful light by... 💡light sponsor💡 @marshydro_amazonled @marshydro12 #marshydro #TSW2000 Low stress training clips- @madmadameplant @madmanplant Www.madmanplant.com Other companies in this grow- @foxfarmsoilandfertilizer - 100% ffof @acinfinityinc - fabric pots @generalhydroponics - flora trio line @vivosun.official - inline exhaust fan @opulent_systems - 4x4x80 grow tent @growerschoiceofficial - 1 Northern lights seeds @inkbird_official - temp and rh controls Her feed has been ph water 4 the week. Well stay posted 4 wet and dry weights!! Thanks 4 the support and checking out my page. Best of luck and Happy growing!!🌱💚💨🔥🔥🔥

The ladies are starting to stretch and flower. We also did a defoliation and lollipop session at the end of week 2 flower. Canopy and bud sites look good and are a plenty. Looking forward to next couple of weeks.