My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

So this was my First Grow ! I am happy with the results- yes it didn’t yield alot only 40grams (1 plant). The buds arent so dense maybe cause of the strain or maybe the light - the overall color of the flowers is very stunning. The purple hue is simply special and unique. During the grow the temperature was getting too high sometimes reaching 30 celcius :/ this could be the result that affected the yield - in this grow I didn’t gave the plant alot of nutrients since I didn’t want to over feed and ruin my first grow. Also during drying I decided to switch on my dehumidifier and caused the temperature in my room to increase around 27 celcius and humidity down to 45% ( yes its alot ). And so it caused the buds to dry quickly ( yes its bad ) another step i Did is Curing , there is a picture of the jar. I am keeping the humidity in the jar around 55% to 65% keeping it in a dark place. And so Yes - For sure there is room for improvement. Overall i enjoyed growing this strain and definitely i will be growing it again in the future. 💜💨

They could be in pot for 1 more week but I really need my weed right now 😂 100% cloudy but no amber

Very Happy plants, soaking up the rays. When you place your face near the buds you get an amazing sweet fruity aroma, the leaves smell like skunk and cheese. Each plant is getting 10 liters every 4 days. I water with 6.3ph tap water twice for every time I add nutrients. I use Alaska fish fertilizer when its been a few times since a nutrient feed but the plants are showing no deficiency's. They are Fed by a drip system which takes 16 hours to deliver the 10 liters to each plant, this works really well in the Fabric smart pots. I use metal racks to keep the plants off the ground then place a drip tray underneath each plant. When using the drip system very little run off occurs. I added a spider farmer sf-1000 to the room, it is full spectrum and ir, and is great for getting the corners of the room or the less dense areas of the canopy.

This week I Supercrop all the plants and spred them out over both lights. One more week of Veg and they are ready to explode Check me out on Instagram @growmorestressless

Tuve improvisar y arriesgar una plaga, con unos guias de sauce que tenia de una poda del jardin, no le logre guiar par a toda la carpa pero me servirara para cosnstrastar los resultados, ya se ven muchos mas tricomas consireblamente.

Day 50 from seed and this plant has exploded compare to the slow growth at the start. I doubt its ready for harvest by week 8 like it states on the brand. But a few more weeks and should be good. I hope i can get a few ounces of this. Ill update by week 8! One love

Welcome to Bud Boutique Grow Diary - really appreciate all your love and support :) Dont forget to check out my other current grows! 🗓️ This Week: - LETS CHOP IT ✂️✂️✂️ - beautiful big bud structure - smelling amazing - last trichome check again after 48hrs Darkness - looking perfect!! - Now lets dry inside the tent for 12-14 Days - Trimjail soon Thank you for still staying with me 💚 ___________________________________________ --- 🌱 Strain --- 🏷️ OG KUSH by Barney's Farm https://www.barneysfarm.com/og-kush-646 ---💡 Lighting --- 💡LUMATEK ZEUS PRO 600 https://lumatek-lighting.com/zeus-600w-pro-29/ --- 🥗 Nutrients and Feeding * 🍸 PLAGRON Algae Baseline grow/bloom + Additives: Power Roots, Sugar Royal, Pure Enzym, Silic Rock, Power Buds, Green Sensation * 📅💪 baseline grow/bloow: 4ml/l & additives: 1ml/l each https://plagron.com/de/hobby/produkte --- 🏭 Grow Setup --- * 🏠🌿 Indoor: Homebox 120x120x200cm (4x4) * 📐🌀 PrimaKlima exhausting Fan 1180m3/h (running on 60-80%) & Can Light Filter 800m3/h & 1x Fanbox 1x Dyson fan for Air circulation https://primaklima.com/de/shop/ventilatoren-de/ec-ventilatoren/pk160ec-tc/ https://canfilters.com/products/filters/ All Likes and comments are highly appreciated!!! don't forget to check out my Instagram: budboutiquee <3 - Bud Boutique

LITTLE BEAST PLANT. GETTING ALL THE LOVE AND CARE I CAN GIVE THEM! 15 Apr. Finally I close a period of my life. With the previous grow. Got switch light. Now running fluence spyder 2i 630w full blast 70 cm from plant. Plenty c02 even if this version of fluence doesn't requires. I'm watering the plant almost everyday. Growing pretty fast. Orange Kusk Cake topping 8 main node. I'm about to turn the first one in a monster. Mainling super cropping lst hst all in. I get to know her more close. 16 everything is running smoothly. Amazing computer keep temperature night and day under control also fan speed. Every tools work in redundancy and equality low speed talking each other. Plant growing beautifully and super lushness. 3 more week of veg. After allingin all of them. Few clones for patient friend. New grower people. Switch to flower. I'm in peace of mind. 17 Apr. I bend and snap practically every node. Poor Lady. I don't have the right of the music. Please remove it if create trouble.

Ever since I turned to mars hydro I have double sometimes nearly quadrupled my yields I first had with eBay lights (meh) I have never looked back since and gained so much respect from the community and the breeders too! And to hold the sponsors I do shows me that I am doing something right and I show appreciation to all who support! And that’s why I take a different effort to be fair and document you all! Even though I’m aware your businesses I just can’t be bias when it comes to the greatest I think you all are! Thank you for choosing me! (No I’m not the only one) just showing the love back as best as I can! 💚💚💚💚💚 thank you

😬😆UnCoNvEnTiOnAl GrOwTh 😆😬 ____________________________________________________________________________________ 🤪 31.3 ... 🤪 1.4 ... 🤪 2.4 ... 🤪 3.4 and here we are finally with a new update. Today I devoted myself to Easter photos. The bonsai are doing very well, this week I start with 2.5 ml / l of Hesi phosphorus. now the flowers are getting bigger and seem to literally want to explode (however much flowers can grow in a bonsai). see you soon with new updates and again thanks to SweetSeeds for these amazing genetics! 🤪 4.4 ... 🤪 5 4 ... 🤪 6.4 ... ____________________________________________________________________________________________ 👀📷🥇 Follow the best photos on Instagram 🥇📷👀 https://www.instagram.com/dreamit420/ 🔻🔻🔻Leave a comment with your opinions if you pass by here🔻🔻🔻 🤟🤗💚🦄Thanks and Enjoy growth 🦄💚🤗🤟

End of week 3, last week we removed bottom node and this week removed last node from bottom tpp, heavy lollipoping for pre veg for 2/3 weeks then flip to 12/12

Grand Daddy Purple auto is doing good. She has acclimated, and started make some good growth. The spots on the tip of the leaves never spread. So that was good. Everything has been looking really good. Nothing more to report at the moment. Thank you ILGM, and Medic Grow. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Seriøst ér @KANNABIA BARE MIT NYE MÆRKE UDOVER @ZAMNISIA SÅ TROR JEG HAR FUNDET 2ud af de 3 frøbanker jeg HAR LYST TIL AT ARBEJDE MERE MED (hvis det er de ville 🖤🧡💙🛐☮️🧡🖤💙💚🔥😇👌

🍓🍌🍧🍨 NEXT YEAR WII RUNIN HER AGAIN IN FULL SUN CUZ SHE NEED ALOT OF SUN ☀️

Bella pianta

Beautiful Plant and good smell - smells like pungent hop with sweet hints. this was her last week. Week 10 she got only 10hrs of light for the first 5 days of the week. Day 66 - Last watering Day 68 - lights off Day 70 - harvest

Hi, I've done this on larger scale in the past, now I do my best to keep within lines of Dutch laws. This is a bit difficult but very doable. I started late with taking pictures, Yesterday I noticed the first white hairs 😀 So I took some pictures today and uploaded them. I grow on very small scale under a desk 😏 so the plant can't grow to large in height. No topping for me, since this is an automatic, I'd like to see that it does. From seed it has been on 12/12 :) also to see if it still will become a nice lookin plant, also to keep it short. I added some chicken fertilizer for extra nutrients, just on the soil. On day 24 (3,5 weeks) I decided to make the plant a bit smaller with LST technique. I hope I didn't break the stem otherwise I'll have to update to HST / LST. Bloow has been set and is going fast. remember, this is from seed on 12/12 lighting.