Week 6 At the beginning of the week I simply finger pressed the new node growth to begin separation and branch direction. 3 days after the last topping, I set up the tomato rings and attempted to tie down train the branches. In retrospect I should have given another day of growth before trying to tie to the new nodes. One plant made the LST training without issues(green tomato ring), however I snapped 2 center colas on the second plant(orange tomato ring). From experience I know that these will not produce tall colas since it happened so late in Veg, so I decided to completely remove them and will monitor for any new growth on those nodes to remove it. So now Im growing an 8set Mainline And a 6/4 set Manifold: Mainline : is a planned topping and defoliation of every cola that makes all topped splits have roughly the same distance to the roots for water ways by removing all other growth along at the same point on any cola. Ideally this helps generate mostly equal buds along the whole cola after each topped split and then the whole plant without having to use scrog nets and just local support ties instead. In a Mainline you remove any other growth that would otherwise need those water ways to grow as well, so Manifold: is the same thing as Mainline but in Manifold you leave a few branches to grow from a lower start point on any cola and then remove everything else. Ideally this gives mostly the same benefit as Mainline but if planned correctly allows you to be finished topping in just 2 separate topping days rather than 3 separate topping days. (I am using it here to correct this late veg mistake in my breaks) The tie downs are positioning 6 of the colas to grow out flat until it can reach the outer bend of the tomato rings where I will allow them to stretch straight up in bloom. 2 cof the colas will have the lowest node of braches growing up in the middle for a total of 4 branches. However in the 8 cola plant I am also training 6 to the outer ring and 2 colas to grow up in the center of the ring. Feed this week was again an auto pot reservoir feed at 400ppm total before add-ins. I used 360ppm Veg concentrate mix (recipe week 2) and 40ppm Bloom concentrate mix (recipe above). However, I also added 1ml/gal of CaliMagic (General Hydroponics 1-0-0) to all feeds and ph balance this week was for 6.3. Next week will consist of more LST training with a simple move of the wires as needed. Feed will increase in total ppm and bloom mix and I plan to reduce ph to the 5.8 range slowly, so about 6.1 this week and 5.9 next before flip on week 9.

she develops very well. I can't wait to harvest it. the smell is very pleasant. No problems so far, or at least nothing visible. Next week, let's see how it performs, these buds are already beautiful and big, high expectations for it if the high humidity here doesn't get in the way!

The GSCs finally hit their flowering stretch and turned from little ninja stars to nice little bushes. I think my low Humidity may have stunted growth a bit - dry winters in so cal. Overall they are doing super nice, and should start stacking and stinking here any day now. I moved them under the big light in the big tent and all the plants are reaching for it like mad. This Mars 2 1600 is a beast of a light.

Everything seems to be going great! She's developing very fast and looks very good,no problems at the moment,let's see how big she gets before flowering starts we're not very far away from that,I'm enjoying a lot growing her,she's an excellent genetic she's being fed just like every single plant that I grow full organically with mycorrizae by florians living organics bat guano by guanokalong,seaweed powder,lactobacillus microorganisms and love,peace guys ❤️💛💚🌱

Beschreibung folgt noch...

Alright, this is the week where I chop the Blueberry Muffin. I'll give the Mimosa another week or two. The Dolato should need another two weeks minimum. On the Mimosa you can see a phsosphor deficency. I've started to add 0-50-30 mineral fertilizer as recommended by the manufacturer.

Hi there This review starts from 1st week of flowering Auto Brooklyn sunrise Looks very small At the end it was a real surprise Hope you will enjoy next weeks

this week ended very nice. I am happy with the growth.

#myseed.ge #soil-N1 #mysun.ge #GROWROOM #MYSEED-GROWROOM it was the transplant week, everything is going perfect <3

Looks like most of the stretch has stopped for both plants. There appear to be two slightly different phenos with one slightly taller than the other. The shorter one seems slightly further along with a bit more flower development and some frost starting to show. I've done a couple rounds of defoliation to get some better air flow and light to bud sites. I also went through and pinched off most of the lower bud sites that were not going to get sufficient light to develop.

Plant at the front #3 has had slowed growth but buds are filling out pretty well now, pretty sure she is a Orange sherbet. Back 2 are on week 3 day 17!!! & Are going Awesome, buds stacking up & down every branch! Going to get some really good Cola's of them! They are loving the 730watts!’ of light, back left plant looks like a Tropicana Cookies, going to do some defoliation on her & tie branches down a bit to open up middle of plant to allow more light & air to lower bud sites.

Another week gone! The first girl on the left finished early, with around 10-20% amber trichomes, so I happily chopped her. The others are close behind and probably entering their final week before harvest. I lowered the RH to 50%, and wow, the aromas really exploded-sweet and intense! One showed her Granddaddy Purple side with beautiful violet hues, while the others stayed true to their Orange Punch phenotype. It's been an awesome run, and next step is flushing the substrate with pure water, CalMag, and Cannazym (pH 6.5). Good luck everyone, and happy growing!

Good. Simple.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

So I had a few issue. I over watered early on, took them out the seed tray early, started feeding them a week or two late, no humidifier yet but they recovered well.

it stands stable in the wind, withstands hot days and continues to grow. but unfortunately still no signs of flowering. she still has 2 months before i move. i hope she makes it. she gets fertiliser every 2 days.

Both started to flower faster than I wanted. I would have preferred another week of veg. Blooming nutes were transitioned during this week. Mimosa is smaller then her counterpart, but is smelling stronger.