Buds are now looking ripe. The trichomes are mostly milky and senesance has set in nicely. Pushing until i start seeing 1 or 2 Amber's and then will induce 48hours of darkness to attempt to increase thc production before harvest

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

12th week of happiness

Welcome to Week 8! All seems to be going well. Two of the Apple Betty plants have fan leaves that are yellowing, but I think it’s because they are getting close to harvest time (about 2 weeks out). I’m trying to maintain a temp of 75-80F and 60% humidity.

Ich denke noch etwas 1 Woche 😉 Seit 1 Woche gibt es nur noch klares Wasser

Mid flower 2 ec

Lampe 100% Ich habe sie entlaubt, und alles was unter dem ersten Netz war kam weg !! Ein zweites netzt habe ich dann ein paar Tage später eingebunden. Hab fast die komplette Fläche ausgefüllt also genau das was ich erreichen wollte!! :)

Wow all her pistils have changed colour! Trichomes all milky! She's absolutely smashed flowering quick! She is as frosty and smelly as can be!! Will be harvested within 24h.

We are at the beginning of the 9th week of flowering. The trichomes are slowly beginning to change color. I'm now going on vacation for 9 days, I couldn't have planned it any shittier. But anyway, I've decided to keep them growing and then probably harvest them straight after my vacation.

Day 1 of flower

📆 Semana 10 Las dos A5 Chaze siguen avanzando sin rastro de hongos y con la humedad completamente controlada. La planta que perdió su punta principal concentra ahora toda la energía en las secundarias, que se están compactando más, ganando resina y mostrando un brillo más marcado. La otra, que mantiene su punta principal, desarrolla una cola dominante muy firme, densa y con ese aspecto clásico de Haze bien llevada. En ambas plantas los pistilos se van cerrando y el color se intensifica. Los tricomas muestran mayoría lechosa con los primeros toques cálidos en las zonas más expuestas, señal de maduración activa. El aroma sube otro punto: terroso, madera seca y especia fina, cada vez más profundo. La ventilación y los riegos están en su punto, aportando estabilidad sin forzar nada en este tramo final. 🌼 Semana de afinado: densidad, resina y aroma consolidándose en cada estructura según su forma. ¡Seguimos creciendo fuerte 💪!

wow! friends my tent 100cm x 100cm x 200cm is completely full! it is my first experience with photoperiod and I was not very prepared for it! They look great with a nice green color. I hope for the best and will continue to work hard! like and leave tips!

2024-07-13 Indoor The Indoor Lady is fading, chrystals are building and well i think the longest Time for her is over and we are slowly coming to an end. OUTDOOR Despite the bad outdoor conditions, the Tangerine Snow stands brave and strong i was feeding her today, and i think i have seen some Preflowers? this week i made Videos from Both, In and- Outdoor Plants BREEDER INFO Tangerine Snow F1 Fast Feminised is a 75% sativa, four-way cross of (Boost x Tangelo) with (Lavender x Power Plant). This Fast F1 hybrid is bred from Cali genetics and boasts great citrus terps, high resin production for extracts, high levels of THC, very good yields and excellent mould resistance. Tangerine Snow F1 Fast can be grown indoors as well as outdoors. Indoor flowering times are between 8 - 10 weeks while harvest time in northern latitudes is during September while in the southern hemisphere growers will be harvesting during March. Recommended climate regions are hot, dry, humid and warm. These are tall, semi-branched plants that grow in excess of 200cm and display a high degree of vigour with very good uniformity. In common with many other heavily sativa-dominant strains, Tangerine Snow F1 Fast offers excellent resistance to mould as well as to plant pests and diseases. The combination of citrus terps and plenty of resin makes thi a very good extract strain with the 'washing' method delivering very good yields of hash. The citrus terpene profile is reminiscent of mandarins and tangerines and also has sweet candy notes. THC production has been lab-verified at a strong 24% while CBD is low. The effect is uplifting and energising, perfect for use during the day and early evening.

Week 2 since they suddenly explored in growth. Since I have a very small tent, I just started the LST. Realised some yellow leave. Maybe due to light burn. Turned down power a bit to 40% and height increased to 25cm

Really noticing the buds putting on size quickly. Still another 4 weeks of flower left to go roughly, will start to slowly drop the nitrogen so they can concentrate on fattening the buds

She is definitely the more bushy of the two, and flowering is filling in nicely. All is well, easy growing 😎

Starting to get close to lights. They love the boosters! Ill post week 3 in few days since this is behind. Took to immune booster and ball buster shmoe krebs cycle booster i made. Now need to give it some cytokinins keep stretch zero. Bubba diagonal is ahead of them all and kush mints barely started flowering probably gonna be a 9 weeker+

Stunted but great 👍 very juicy and smells very nice the second plant hasn’t got any purple and has struggled abit but I think it’s still gonna be good