2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Fastbuds - Mimosa (2x) 08/27 Coming into week 4 Veg, User error stunted them lightly but things seem back on track. Exhale 365 C02 bag introduced last week to setup for early flower. No signs of concern or any deficiencies. Early flowering has begun *Mid-Week Update* *08/30* Both plants have recovered nicely, and placed under trellis net. Introduced NPK Phosphate Purged Pure Blend Pro Grow *End of week recap* Flowering has begun Stopped veg nute feed Introduced Raw NPK Phosphorus and Potassium

Cruise control... Did a rez change, fresh new RO water + added calmag and nutes totalling 750ppm. Not much vertical growth. Buds fattening up now. tied up some of the branches and defoliated a little to allow more light penetration.

Let's harvest ! Let's dry 💚 this was a good week like usual , it was the flushing and final week

Start of week 15 5/20/25 Fed with a gallon of nutrients 5/22/25

👌

Hi everyone 🤗 This week it looked more and more astonishing after opening the box a wonderful sweet smell wafts through the room 👍. A few days ago the Blue Cheese Phenotype 1 was harvested and placed in the dark room :-). This week both Kosher Tangie Kush phenotypes will be harvested 👍. Next week the Blue Cheese phenotypes 1 and 2 will be harvested ;-) everyone else needs a while 😀. I wish you all a nice week, stay healthy 🙏🏻 and let it grow 🌱👍

Incredibile experience!

Last week was our transition week. Pistils are now appearing so let’s call this week 1! Happy with this grow so far. Lots of defoliation and lollipopping to get some airflow...

Going to call these LemonWalker CB? Since it is about 50/50 Chance of being either hight THC or medium THC/CBD first one will go to flower Saturday Looking good definantly tow diffrent growth phenos LemonWalker 1 goes to flower Tomorrow after I take clones

Im really pleased with how these girls are growing..at first i was worried about how small they may be but now seeing how stocky they have got im excited. In total this is my 6th ever run and im so happy with the results.

Semana 0 Del 3 al 9 de agosto Estamos en la semana 0 que va a ser la primera desde el cambio a 12h. Las temperaturas están siendo geniales y los LED ayudan bastante. Estamos en 26°C en ON y unos 24°C en OFF. Vimos que el último riego pudo ser demasiado potente y aprovechamos para hacer varias cosas. • La planta tiene 0 marcas de bichos, ni picaduras ni nada.. parece que los preventivos han funcionado bien, pero no bajamos la guardia. Espolvoreamos la capa de arriba del sustrato con tierra de diatomeas y humedecemos. Como el sustrato ya había bajado bastante, echamos una capa de sustrato ( monkey soil coco) y repetimos volvemos a espolvorear. • Realizamos riego con unos 2l casi. Usamos 0.16 Mammoth P + 1 calmax PH 6,2 EC: 0,4/0,6ms Th20: 23,5°C • Hoy soltamos todo el sistema LST. La idea es la siguiente: dejarle un día suelta descansando para que se estire un poco y luego colocarlas en su posición final. Parece que tiene forma de pino y cada rama esta cogiendo esa forma, por eso quiero estirar la un poco, para que tengan sitio. 5 de agosto Volvems a hacer el LST Pero esta vez con grapas de hierro, aprovechamos para dejar en posición. Mientras la entrenabamos quebrados el tallo central.. 6 de agosto Vemos que se posiciona mejor y aprovechamos para quitar algunas hojas y algún brote pequeño. Hoy a vuelto el calor pero aguantamos en unos 28,5°C de max. La humedad si he es alta, entorno al 70% 8 de agosto Hoy movemos un poco los brotes para que quede menos sombras. Aprovechamos para hacer un riego con 1,5l usando 5ml•l de Delta 9 PH 6,01 EC: 1,2ms Th20: 26,6°C El calor continúa y más o menos aguantamos las condiciones. 28°C y un 66% de humedad. Este riego de Delta 9 le sentará genial, se puede apreciar como siguen estirando los brotes de cada rama para coger su sitio de cara a florecer. Además la planta en general a engordado bastante en cuanto a tallo y ramas más fuertes. 9 de agosto Terminamos esta semana de perforación. Seguimos con el calor y la humedad, en ese sentido a sido un mal día. De temperatura rondamos los 28°C con una max de 30 y mínimas de 24,5°C (en OFF)*. La humedad a andado de 60/70%, esperamos que las condiciones mejoren cuando la flora sea mas avanzada. Hoy realizamos ultimo preventivo, unas 6h com el quemador de azufre para después de ventilar un rato hacer un foliar con 15ml spruzit + 2 aceite de neem + 2 un producto de prueba. Las sensaciones son tremendas y la verdad que nos lo estamos tomando con mucha ilusión porque esta quedando preciosa y respondiendo como un Jabato. El juego a empezado amigos, a partir de ahora empieza lo buenoo.

The royal crematic have some calmag deficit so i give biobizz calmag. The wedding cake looks and smels awesome, maybe 1 or 2 weeks and she is ready to harvest. Higher the sunlight evo from 60 to 80 % power.

Hello people... So heres the new strains Gellato 41 by SEEDSTOCKERS. New setup with Heavengrowlights 320w, lets see hoy they do. left a couple of timelapses for the germination process cheers feel free to coment

Week 4 flower: all 3 plants left in the tent are pollinated - it's hard to tell to what extent but bud formation has slowed down 😞. Middle plant (Mimosa Evo #2) is definitely the strongest pheno and its trichomes smell like tangerines which is really nice! We did an intense defoliation on Day 24 of flower in an attempt to lower humidity and increase airflow but RH is still dangerously high at 80% because of the current weather. Also gave them a bloom PK top dressing on day 26 because canna coco does not provide a PK boost in their coco nutrient line. Hope to see the buds dense up over the next few weeks without too many seeds ...

Flower Week 7 Cherry Poppers almost done i think 1-2 weeks to go checkin every 2-3 days the tricomes HerzOG needs probably 2weeks more

10/21 Day 23 Well may be seeing some improvement on Brandy and Maggie from the drench and mag foliar spray. Will spray again tomorrow. Layla is just lovely and holding off another day on watering, temps have lowered and humidity is up so drying is slow. Just dont get it but I have decided to go full Coco coir from here on, something aint right 10/22 Fed Layla Big Bloom 3tsp - Fish Mix 1tsp - CalMag 1tsp - Recharge 0.5tsp - Fish Sh!t 0.5tsp / Gal at 6.5 ph for 0.5 gal Brandy and Maggie seem to be improving, had to train and gave another foliar spraying nothing else done. 10/24 Update New Pics - note new growth Plants beginning to drink more and Maggie showing most improvement. Afraid Brandy will stunt but sure that was going to happen a week ago. Leaf structure is improving greatly. Prob will feed in a day or two and another foliar spray at the same time 10/26 Day 28 Finally fed all three girls - nothing much has changed appearance wise and pics tomorrow on new week. Including initial dose of bloom nuets as hairs are showing strong - no foliar spray until Sunday. Feeding: Cal-Mag 5ml - Big bloom 15ml - Fish Mix 5ml - Bio-Bloom 5ml - Fish Sh!t 5ml / gal @6.45 ph for 1 gal (Layla) 0.75 gal (Maggie) 0.5 gal (Brandy) as they are drinking at different rates unfortunately. Final PH is taken after all nuets added, makes a difference. NOTE: Since this veg cycle is ending and the plants will be moving to the Flowering tent and this tent will be doing drying duty for the LSD-25 for a few days, time we were getting the Growit Coco coir ready for the Green Crack grow in a week or so. Hydrated the first brick (2 cuft hyd) and am single buffering the coco with 30 ml of cal-mag in four gallons of water for 24 hours. This will also serve as a safety wash just in case the factory lies :) Its supposed to be pretty good coco, little if any peat or croutons I can see. Will do second brick when this one finished and I can store Looking at using a different system this time around. Hope yall follow along Went ahead and posted the daily pics of the problem children just for giggles.

🌸🍏✨🍭🌸🍏✨🍭🌸🍏✨🍭🌸 Hi and welcome to another Kannabia grow! This time I’m running their Apple Fritter (feminized photoperiod). She’s going to veg under 24hr light and be manifolded. Still cleaning the rest of my Candy Cream GF (11.04.25) — going to sow the seed tomorrow (12.04.25) *been lazy, sowed on 14.04.25 🌸🍏✨🍭🌸🍏✨🍭🌸🍏✨🍭🌸 --- 💭❗💭❗💭❗💭❗💭❗💭❗💭 ❗Events & thoughts worth noting❗ 💭❗💭❗💭❗💭❗💭❗💭❗💭 12.04.25 (GW1) – Cleaning + flushing the old coco coir. Might need to add another brick. *No new brick needed ✅ This is how I recycle my coco coir — fast, cheap, no BS. 🌿 Harvest plant ✂️ Chop roots small — they stay in for structure (organic perlite) 💦 Hot water rinse — remove salts & dust 🍶 Pre-soak with light feed:   Micro 10ml   Bloom 0ml   GreenBuzz 10ml   Cal-Mag 60ml   FFJ/FPJ 10ml (new disgusting batch)   pH down (citric acid) ♻️ Media stays — Roots stay — Back in service. 14.04.25 (GW1) – Planted seed in final pot ✅ 16.04.25 (GW1) – Did last tent clean up + setup ✅📸 17.04.25 (GW1) – Seed germinated 🎉📸 — roughly 48hrs in final pot. Solid start👌♥️ 23.04.25 (VW1) – Minimal burnt tips 📸 — not progressing, not a concern. 26.04.25 (VW1) – Started using the new batch of FPJ/FFJ https://growdiaries.com/diaries/266849-grow-journal-by-yan402 30.04.25 (VW2) – Increased TriPartMicro & GreenBuzzBloom 10ml → 15ml 03.05.25 (VW2) – Increased TriPartMicro again 15ml → 20ml, topped the plant, and added final layer of clay pebbles around the base, trimmed side branches and did LST in preparation for "manifold" 📸 08.05.25 VW3 finished manifold📸 13.05.25 VW4 Increased TriPartMicro to 30ml and GreenBuzzBloom to 20ml. 15.05.25 VW4 Done defoliation and LST 📸 18-19.05.25 VW5 pruned all the shoots bellow my "mains" and did a full defoliation📸 24.05.25 VW6 increased GreenBuzzBloom to 30ml 04.06.25 VW7 did a cleanup📸 11.06.25 VW8 increase TriPartMicro to 40ml 17.06.25 (VW9) – Final structure pass 💈🌿 Did a clean perimeter prune + removed weak shoots. Ended up with 12 tops, was aiming for less, but she made the call Didn’t fight it, just shaped it the best I could Airflow’s good, structure’s stable 🛑 No more cuts until post-stretch Pics coming shortly 😘 Flip coming soon — we’ll see how she handles it. 20.06.25 VW9 Switched lighting to 12 hours, may the stretch begin 🤞 22.06.25 VW10 Did a good LST session,made some pics, and came to some conclusions and a small change of plans: Originally planned for 8 mains — long, spaced colas and maybe a couple stronger “titans” if she wanted to go that way. But she’s showing me something else, and I’m not here to fight her — just guide her. Now after stretch, I’m keeping side shoots only if they: Fill real canopy gaps Don’t crowd neighboring tops (minimum 15cm spacing) Aren’t growing into walls or toward the next plant Anything too close, too low, or heading into shade gets removed. No point forcing it. Looks like I’ll finish with 14–16 solid tops, depending on how she settles. I’m just trying to give each one enough light and space to stack properly. No overcrowding, no larf — just letting her do her thing with a bit of structure. Increased Tri Part Micro to 50ml as well. 28.06.25 VW11 one week since I flipped to 12/12 and she is stretching nicely, a bit shy in showing pistils compared to the Fantasy Feast regulars I have in the same tent. 29.06.25 VW11 increased GreenBuzzBloom 30→ 60ml 06.07.25 FW1 TriPart Micro: 50→ 30ml TriPart Bloom: 0 → 20ml Home-made FFJ/FPJ (Watermelon + Pumpkin): 10 → 30ml 12.07.25 FW1 GreenBuzzBloom 60 →40ml, TriPart Bloom: 20 → 60ml, fpj 30→60ml 19.07.25 FW3 Got some bleached tops, been out and about at job interviews and didn't notice a last minute stretch spurt 😭, should recover fine though plenty of time left 😁 22.07.25 FW3 TriPartMicro 30→20ml , TriPartBloom 60→80ml , GreenBuzzBloom 40→50ml 24.07.25 FW3 Cal-Mag 60→40ml 🌱💦🌱💦🌱💦🌱💦🌱💦🌱 🌿 Day to day tasks & actions 🌿 🌱💦🌱💦🌱💦🌱💦🌱💦🌱 19.07.25 FW2 – Fed 5l of #1 → 2l runoff 20.07.25 FW3 – Fed 5l of #1 → 2l runoff 21.07.25 FW3 – Fed 5l of #1 → 1.5l runoff 22.07.25 FW3 – Fed 5l of #1 → 1.5l runoff 23.07.25 FW3 – Fed 5l of #1 → 1.5l runoff 24.07.25 FW3 – Fed 5l of #1 → 1.5l runoff 25.07.25 FW3 – Fed 5l of #1 → 1.5l runoff 26.07.25 FW3 – Fed 5l of #1 50% strength→ 1.5l runoff (*RUNOFF reused for tomato plants) 🍶💧🍶💧🍶💧🍶💧🍶 💧 Nutrients in 30L #1 🍶💧🍶💧🍶💧🍶💧🍶 💧 TriPart Micro: 10 → 15 → 20 → 30 → 40 → 50ml → 30 → 20ml (0.67ml/L) 🍶 TriPart Grow: 0ml (0.00ml/L) 💧 TriPart Bloom: 0 → 20 → 60 → 80ml (2.67ml/L) 🍶 GreenBuzz Bloom: 10 → 15 → 20 → 30 → 60 → 40 → 50ml (1.67ml/L) 💧 Cal-Mag: 60→40ml (1.33ml/L) 🍶 Home-made FFJ/FPJ (new batch): 10 → 30 → 60ml (2.00ml/L) 💧 pH Down: Citric acid (buxXtrade) 📦 TOTAL: 250ml per 30L 🔬 8.33ml/L 🍶💧🍶💧🍶💧🍶💧🍶 ⚙️✂️⚙️✂️⚙️✂️⚙️✂️⚙️ ✂️ Tools & equipment ✂️ ⚙️✂️⚙️✂️⚙️✂️⚙️✂️⚙️ ✂️ 2× MarsHydro SP3000 ⚙️ MarsHydro 150mm ACF Ventilator ✂️ Trotec dehumidifier (big unit) ⚙️ Mini no-name dehumidifier ✂️ Kebab skewers (LST – stainless) ⚙️ Wire + roast skewers (LST assist) ✂️ Scissors (HST) ⚙️ Vacuum (for spills & cleanup) ✂️⚙️✂️⚙️✂️⚙️⚙️✂️⚙️✂️⚙️✂️⚙️ --- 🍏🍪🌬️🍬🍏🍪🌬️🍬🍏🍪🌬️🍬 Apple Fritter (Kannabia Seeds) 🍏🍪🌬️🍬🍏🍪🌬️🍬🍏🍪🌬️🍬 Species: 50% Indica / 50% Sativa Genetics: Sour Apple × Animal Cookies THC: Up to 25% Effect: Euphoric, relaxing, creative Flavor: Sweet, fruity, pastry notes Flowering: 56–63 days Resistance: High Indoor yield: 450–500g/m² Outdoor yield: 600g/plant Structure: Strong, bushy, dense buds