Bud quality is amazing, tons of frost and very sticky. Plants didn't get big colas unfortunately it was more smaller buds everywhere but still turned out good. Very tasty, could produce lots of hash also.

Legend Timestamp: 📅 EC - pH: ⚗️ Temp - Hum: 🌡️ Water: 🌊 Food: 🍗 pH Correction: 💧 Actions: 💼 Thoughts: 🧠 Events: 🚀 Media: 🎬 D: DAY, G: GERMINATION, V: VEGETATIVE, B: BLOOMING, R: RIPENING, D: DRYING, C: CURING ______________ 📅 D36/V32 - 21/05/24 ⚗️ EC: 1.0 pH: 6.0 🌡️ T: 20°C H: 70% 🌊 🍗 💧 💼 LST 🧠 🚀 🎬 1 TL in her new home and one TL of LST job ______________ 📅 D37/V33 - 22/05/24 ⚗️ EC: 1.0 pH: 5.4 🌡️ T: 20°C H: 70% 🌊 🍗 💧 💼 LST 🧠 🚀 🎬 1 TL video and one TL of LST job ______________ 📅 D38/B01 - 23/05/24 ⚗️ EC: 1.0 pH: 5.3 🌡️ T: 20°C H: 70% 🌊 2L 🍗 💧 💼 🧠 As I saw clear signs of blooming, I need to pass to bloom phase so I put B01, in the header. 🚀 Clearly she's starting flowering 🎬 1 TL video and 1 LST video ______________ 📅 D39/B02 - 24/05/24 ⚗️ EC: 1.1 pH: 5.3 🌡️ T: 20°C H: 70% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video and 1 LST video ______________ 📅 D40/B03 - 25/05/24 ⚗️ EC: 0.2 pH: 5.3 🌡️ T: 20°C H: 70% 🌊 15L 🍗 💧 💼 Flush for two days and then flowering nutes 🧠 I think no more LST, she's now full blooming 🚀 🎬 1 TL video ______________ 📅 D41/B04 - 26/05/24 ⚗️ EC: 0.2 pH: 5.3 🌡️ T: 20°C H: 70% 🌊 🍗 💧 💼 Flush for two days and then flowering nutes 🧠 I think no more LST, she's now full blooming 🚀 🎬 1 TL video ______________ 📅 D42/B05- 27/05/24 ⚗️ EC: 1.1 pH: 6 🌡️ T: 20°C H: 60% 🌊 15L 🍗 Calmag - Bloom A-B - Bud Candy - B52 - Big Bud - Seaweed 💧 💼 👉👉👉 Changed res and added nutes 🧠 I think no more LST, she's now full blooming 🚀 🎬 1 TL video

06.06.20 (31 DÍAS) hice un ajuste de los ganchos del L.S.T. las ramas secundarias están creciendo bien. Antes de que entre en floración voy a aplicar una defoliacion. 10.06.20 Agregué top crop auto, no lo había puesto por que no tenía y con el green explotion le di un último empujoncito de nitrógeno para el futuro.

Stretched a bit. Ramping up their nutrients. She’s doing well! She had a prominent main cola. She’s not as big as the other testers. Bu tv seems to be the most frosty so far

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hey now, I hope everyone is having a great weekend, it was a good week for the ladies in the 5X5, they are all starting to stack flowers and really starting to distinguish their own smells. The Bubble Runtz has the most indica dominant looking colas and smells absolutely amazing. The Sundae Driver is looking like she is gonna have the fattest colas and be the biggest producer. I am really hoping I get the red phenotype of the Red Hot Cookies, and both the Green Crack and Durban Nights are showing the most Sativa characteristics and looking great. I now have the Medic Grow Light cranked up to 100% in F1 mode and am also using the UV/IR for 30 minutes per day prior to lights out. I am running a 12/12 cycle and feeding the same mix of GH nutrients which include Silica, CalMag, MaxiBloom, and Bloom Booster, with additional food grade Hydrogen Peroxide in the reservoir to keep algea growth to a minimum. I mix one 5 gallon bucket of nutes per day and clean my reservoir once per week. My pump is set to auto feed the plants every 5 hours after lights on, and I have a gravity self draining runoff setup I built. Not much to do from here on out, just keep the reservoir clean and full, a little defoliation, and 5 minutes per day to mix a bucket of nutes.I hope they get fat in the next few weeks and then I have about 7 people lined up to help me trim, I will throw everyone two zips for the help. hoping to do the wet trim in a day. I hope everyone has a great rest of your weekend, Thanks for stopping by, Stay Safe and Blaze On!!! 💪 Website: https://medicgrow.com/ https://growdiaries.com/grower/medicgrowled

Well I’m pretty excited about this being my first auto grow. These things are really doing awesome I’m pretty impressed I don’t have any experience with them but I feel like they’re all pretty big for their size all of them are really starting to stack up bud. One of the jacks seems to be a few days behind the others in the flowering process. The jacks are both a lot Bashir and flatter the bubblegum and OG a really stretching out nice I love that shape of plant. Soil pH and everything keeps testing good no big issue so far I think one of the plants may have had some small nutrient lockout last week but I flushed it out good. I’ll update this week again soon or maybe not I don’t know thanks for stopping bye be safe ✌️.

It was Christmas this week so I haven’t been giving the girls that much love just water only. I did make a compost tea today that I will be giving them tomorrow P.zkittles x temple kush- they are getting frosty. Pheno #2 is definitely shining over #5. No more diesel smell more of a fruit basket Purple punch- the bag appeal on these hoes are unreal. I am just disappointed on the yeild department. When we ran this outdoor she really produced. I’m wondering if it had to do with a phosphorus deficiency. Thats what I thought I had. It could just be the genetics on how purple she got in early flower.

Had a great week. These babies are still stretching quite a bit and I see pistils everywhere. The next couple of weeks should show some actual nuggies popping up.

Día 42 (27/02). Riego 1,25 Litro H20 + Wholly Base 2,5 ml/l + Solid Green 1,5 ml/l + Rise Up 0,5 ml/l de Gen1:11 TDS 898 PPMs - pH 6,5 (mínimo ajuste con pH+ para subirlo desde 6,2) Plantas sedientas con un intervalo de riego cada 4 días. A partir de ahora regaré cada 3 días Día 43 (28/02) Las ramas crecen muy rápido y se van colocando para recibir el máximo posible de luz. Es impresionante su evolución día a día! Día 44 (29/02) Han crecido 17 cm desde que cambié a 12/12. Alucinante! 😍😍😍 Día 45 (01/03) Riego 1,25 Litro H20 + Wholly Base 2,5 ml/l + Solid Green 1,5 ml/l + Rise Up 0,5 ml/l de Gen1:11 TDS 891 PPMs - pH 6,25 Añado un poco de substrato al top y a los bordes de la maceta antes de regar, porque se ha compactado ligeramente. Día 46 (02/03). Las plantas siguen su crecimiento imparable. 3 cm al día 😍 💦Nutrients by Gen1:11 - www.genoneeleven.com 🌱Substrate PRO-MIX HP BACILLUS + MYCORRHIZAE - www.pthorticulture.com/en/products/pro-mix-hp-biostimulant-plus-mycorrhizae 🎚️Controlled by TrolMaster TCS-1 Tent-X System Main Controller - https://www.trolmaster.com/Products/Details/TCS-1 Es genial tener el TrolMaster TCS-1 Tent-X ya que puedo tener una visualización rápida de la temperatura y la humedad en tiempo real, y también de las últimas 24 horas y de los últimos 7 días (week) Así puedo ir ajustando la extracción para tener una VPD ideal, y tener una pantalla que te indica si estás en rango, en función de si estás en Fase Vegetativa, Floración o Stress Es genial a su vez tener un tabla / grafico visual de VPD en función de las fases. Mira las últimas fotos para ver esta tabla tan interesante! ("Trolmaster VPD Graph" photo)

They liked the flush. 🚽 Nothing crazy this week just topped off the buckets,added nutrients,& adjusted the ph.🤷‍♂️🏽 New growth is healthy looking. 🌿

Super sticky and super dense flowers ,started flushing now for the next 2 weeks

Overall very happy with the grow. Got 24oz of very dense nugs! I also managed to get 2oz of less dense but still pretty bag-appeal, and a shit ton of trim which I made into tidy ass brownies, which have made typing this incredibly difficult😂! Both of these strains are definitely bed time strains. The Critical kush is has the usual overwhelming kush taste, however the peyote(purple nugs) have a very sweet / flowery taste and smell. Disappointed I only done 1 peyote but more than happy with the critical. Would definitely grow both these strains again!

Looking good Day 18, starting to flower

Día 16. Por ahora las plantas siguen en proceso de estiramiento. Hoy hice una defoliación fuerte. Esta etapa me pone ansioso porque quiero ya empezar a ver los primeros pelos de las flores. Pero viene bastante lento el tema. Supongo que voy a tener que esperar un poco más que 10 semanas para la cosecha. Pero tampoco me desanimo.

05/07/2021 Big bud is recovering, she is now more green with buds starting to grow. My goal was to harvest all at the same time but Big Bud will need extra weeks 😐 Now i have problems with #2, very yellow with brown spots, i raised the nutes, but nute burn started to show up, gave her a bit calmag and cut the nutes to half. I think she needs more 2/3 weeks to harvest, just like #1 and #3.. #1 and #3 have no big problems, only big buds 😄

Je lui donne 2 litres d’eau par jour