Week 8 in the books, cruising to the finish line two more hard watering each and 48 hrs of darkness starting Friday. Genetics: Natty Roots Seed Co Instagram: @green_house_lab

Flushen und dann Abhängen mit den Buddies :))

Had a MAJOR fungal gnat infestation. I was overwatering. Got doctor doom and blasted the soil with it twice. Then worked cinnamon into top layer and let pots dry out a lot more. Started watering in 2L rather than 3.

Oh boy, what a week have they got Sick PH was very high. Didn’t know what to do so the last week and a half. I’ve been flushing them bringing the pH down little by little trying to go gentle eventually the last flash show PH at 6.8. Which is OK anything between six and seven is considered optimal range for cannabis plant. Today I shut Lemon cherry Gelato. PH after runoff is 6.8 I used 1gal added 4ml of Gold leaf

_____📅 Week 17 | 📅 Day 119 | 🍂 Harvest after the 9. week 🍂 ______ Day 119 🌞🍂🍁💐 - Sorry for the photos, they didn't turn out well in my opinion, but I only had limited time and the Biscottis had to be harvested urgently...I'll try harder again with the Amnesia. - Yield is okay...but the buds didn't get even light either...there were too many plants or too long a veggie phase... - Biscotti number 2 is the smallest of all the plants...also had the lowest yield...This was the plant without side light...

Everything going good.. Humidity was a little high so installed a dehumidifier through the wall. Much better now. I got rid of the Gelato it was just bad from jump and then started growing some weird stuff on leaves.. Wasn’t risking the others catching something. But they all seem to be growing good.

Bastante bonita, buen producción a pesar de que le dio botrytis en la copa principal y tuve que cortar por prevención. Un olor muy original, honestamente me gusto mucho, la considero fácil de cultivar con un plan de nutrientes medios.

8/12/21- Week 19 is here and the 2nd day of flushing. I called it yesterday. Buds are looking good but the plant herself isn't. She's had her run and now it's time to prep for harvest. Really looking forward to this. I'll flush for 3-4 day, but most likely the 3. Then the happy sad part, chop chop.

Let’s Go Day 52 of 🌺!! At 101 days old , these girls are looking an smelling oh so amazing! 2 of the Pre 98 Bubbas and both Dirtyblondes started getting flush on Saturday, so they will continue flush the rest of the time for 12 days an they will be ready for 48 hrs of darkness. While the 2 other Pr 98 Bubbas will remain on same feeding through rest of this week a most likely start getting flush by next week!! Hope you all enjoy an have an amazing productive day as well as great holiday weekend, happy holidays keep them eyes peeled for next weeks updates , Cheers y’all 😶‍🌫️💨💨💨💨💨🤙🏻

Day 42 first day of the week I turned the Growers Choice ROI-720 up to 30% its using 216w at 240v. Day 44 pictures were after some simple hand bending lst. This week we've seen alot of growth in all the plants. They need water about ever 4 days and between all 4 they take a gallon and a half untill the get a little run off out of the bottom. Roots are starting to come out of the bottom of the pots. Day 44 I noticed some spots on plant bs leaf I think it was from a nat or something I saw in the tent. And same day plant d has very slight yellowing on the tips of the newest growth. Think it's a deficiency of some sort, I'll look into it. Day 45 Transplanted into 5 gallon fabric pots with fox farms ocean forest soil. Didn't water today because the root balls where still pretty moist. Raised light to maintain 24" about the canopy. Day 46 Watered all 4 plants took about 4.25g of water. PH 6.3 @ 73° F. Day 47 Think I shouldn't have watered yesterday plants A, B, and D are drooping. Plant B has some light brown/yellow spots on the middle growth and very slight yellowing on the tips of the newer growth. Plant D has different spots dark red/brown almost burnt orange on middle growth and yellowing on the tips of the leaves. Weird how plant C just doesn't care what I do to it it just keeps getting bushier and wider leaves, never yellowing or drooping. Day 48 None of the plants were dropping the evening, and the spots and yellowing havnt gotten any worse. They grew alot since I measured yesterday.

OMG . SMELL THIS BUDS ARE AMAZING. Verry strong buds .💚💚💚💚💚💚

Day 48 Gelato - day 48 - Defoliation was done. The broken branch healed and sprouted new branches. She cannot boast much about his height. But it is very beautiful, I hope we will grow 50 centimeters in flowering 👌 Day 44 Peyote - The growth process continues unabated. I am waiting for the desired size. 8 days after flowering it didn't grow as much as I thought it would, so we went back to the growing season. 👽 Day 49 Peyote - I decided not to cut the leaves anymore after much defoliation. It easily tolerates strong stress easy to grow.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

DAY.54 👃🏿🔥🍦🍨🍧

Day 15 - Sun. 7/29/18 N/A Day 16 - Mon. 7/30/18 9:45PM 1. Photo Update Day 17 - Tues. 7/31/18 2:00AM 1. Soil pretty dry, cup pretty light. Watered with 5.8 pH RO/DI water Day 18 - Wed. 8/1/18 1. Time Lapse video Day 19 - Thurs. 8/2/18 2:00AM N/A Day 20 - Fri. 8/3/18 3:00AM 1. Soil pretty dry, cup pretty light. Watered with Fox Farm Big Bloom 5.8 pH RO/DI water Day 21 - Sat. 8/4/18 N/A

The fourth week of growth has begun, and our FBA 2503 from 42Fast Buds is thriving. After the transplant into a 10-liter pot, the plant has adapted well and continues to develop strong and healthy. Despite being an autoflower, we're experimenting with a 12/12 light cycle, and so far, the results look promising. The temperature remains stable, reaching up to 30°C during the day, with an average of 27°C–28°C. Humidity is maintained between 60% and 65%, providing optimal conditions for growth. The plant has grown noticeably, now standing between 20 and 25 cm, with well-developed foliage. We've started applying low-stress training (LST) to shape its structure, encouraging better light penetration and maximizing potential yields. Additionally, we continue to feed our beauty with Xpert Nutrients, and the results are already visible—strong, vibrant growth and lush green leaves. Everything is on track, and we’re excited to see how our experiment unfolds in the coming weeks!

My nine Week 7 flowers are thriving under the new GrowPro Solutions under-canopy lights. The lights have really brought out some amazing colors in the buds. It's so cool to see how much they've changed in just a day! I'm excited to see how they develop even more in the coming weeks

Week 10 - Flower Week 3 Ambassador Cartwright Ambassador Cartwright by IRIE Genetics Transition to Flower Afterglow X Arise (Clementine X Arise) X Arise ((Tangie X Lemon Skunk) X Arise) X Arise ______________________________________ Start of Week: Saturday 10 September 2022 AC3Q 57:F:3:1 to 17 September 2022 Summary: Plant HeightBeginningOfWeek: [ 20, in] Plant HeightEndOfWeek: [ 26, in] *TBD* Temp: 74.8 RH: 70.7 VPD: 0.88 kPa # need to get into the 0.9 -> 1.1 Early Flower Autofertigation - Enable - [ ] Growtek RINGS Calibrated - [ ] Growtek RINGS INSTALLED - [ ] Growtek OPERATIONAL LightSchedule: 12/12 amt: [ 1300, ml] # period: [ 360, min] frequency: [1, day] times: { first: 2100, second: 0300 } # Auto Only!!! Otherwise 1.3 @ 2100 Environment: EC Targets: # rel. | abs (rel + source) ECseedling: [0.4, 0.5] [ 0.7, 0.8] ECearlyveg: [ 1.1, 1.4] [ 1.4, 1.7] EClateveg: [ 1.2. 1.5] [ 1.7, 1.8] ECearlybloom: [ 1.1, 1.4] [ 1.4, 1.7] EClatebloom: [ 1.3, 1.6] [ 1.6, 1.9] ECripen: [ 0.8, 1.1] [ 1.1, 1.4] ECflush: [ 0.0, 0.2] Seedling Early Veg Late Veg Early Bloom Late Bloom Ripen Flush 400* – 500 1100 – 1400 1200 – 1500 1100 – 1400 1300 – 1600 800 - 1100 0 - 200 Fertigation Daily: - {pid: 4, amt: 1300 ml, ec: 2.0, pHRange: [ 5.5, 6.5], time: 2130} - {pid: 7, amt: 1300 ml, ec: 2.0, pHRange: [ 5.5, 6.5], time: 2130} FertigationtheRootedLeafFullRatios: # Flower Week 3 Primer A: [ 10] Primer B: [ 10] CalMag Fuel: [ 6] Silica Skin: [ 4] Lush Green: [ 4 ] Root Anchor [ 6] Peak Bloom [ 4] Resin Bloom [ 2] Last Week of Foliar FoliarMonday: Solar Wind: [ 2.5, ml, 500, ml] Silica Skin: [ 2.5, ml, 500, ml] FoliarWednesday: CalMag Fuel: [ 2.5, ml, 500, ml] Lush Green: [ 0.63, ml, 500, ml] FoliarFriday: Solar Wind: [ 2.5, ml, 500, ml] Peak Bloom: [ 0.63, ml, 500, ml] # Cease Begininning Flower Week 5 NEW WEEK Week's Plan - (Added Veg week to allow SuperCropped Mains HEAL.) - [ ] Install Growtek Fertigation w/ 8” Rings - [ ] Run Feed Lines & Cut to Optimal Length - [ ] Calibrate Feed - [ ] Install Drain Lines, Cut to Optimal Length - [ ] Notch Drain Tube end to eliminate vacuum against drain pan _____ Sat 10 Sep 2022 AC3Q 57:F:3:1 # PM - [x] As Below Fertigation: pid: 4, amt: [ 1300, ml], ec: 2.2 pid: 7, amt: [ 1300, ml], ec: 2.2 LightIntensity: [ 824, µMol/m2/s2] LightDLI: [53.4, mol/m2/d] Sun 11 Sep 2022 # AM IS 9PM Local TIme, the Night BEFORE. AC3Q 58:F:3:2 NOTE: - We’ve added a couple inches removing all restraints, and having flipped last week. As frequently observed, Both plants were clearly vibrant and vigorous after a nights sleep after restraint removal. - Zymes/Bonners/Ascorbic Acid - Soil surface, container sides, and container bottom (exposed roots) - [x] As Below Fertigation: pid: 4, amt: [ 1200, ml], ec: 2.4 pid: 7, amt: [ 1000, ml], ec: 2.4 # AM - [x] Complete Previous Weeks GrowDiaries - [x] As Below Fertigation: pid: 4, amt: [ 1200, ml], ec: 2.4 pid: 7, amt: [ 1200, ml], ec: 2.4 # PM - None Mon 12 Sep 2022 AC3Q 59:F:3:3 # AM - [x] As Below Fertigation: pid: 4, amt: [ 1200, ml], ec: 2.4 pid: 7, amt: [ 1200, ml], ec: 2.4 - [x] As Below FoliarMonday: Solar Wind: [ 2.5, ml, 500, ml] Silica Skin: [ 2.5, ml, 500, ml] Tuesday 13 Sep 2022 # AC3Q 60:F:3:4 # AM - [x] As Below Fertigation: pid: 4, amt: [ 1200, ml], ec: 2.4 pid: 7, amt: [ 1200, ml], ec: 2.4 Wednedsay 14 Sep 2022 # AC3Q 61:F:3:5 #AM - [x] As Below Fertigation: pid: 4, amt: [ 1300, ml], ec: 2.2 pid: 7, amt: [ 1300, ml], ec: 2.2 - [x] Apply as Below FoliarWednesday: CalMag Fuel: [ 2.5, ml, 500, ml] Lush Green: [ 0.63, ml, 500, ml] Thursday 15 September 2022 # AC3Q 62:F:3:6 # AM - [x] As Below Fertigation: pid: 4, amt: [ 1200, ml], ec: 2.2 pid: 7, amt: [ 1200, ml], ec: 2.2 Friday 16 September 2022 # AC3Q 63:F:3:7 # AM - # PM - [x] As Below Fertigation: pid: 4, amt: [ 1400, ml], ec: 2.4 pid: 7, amt: [ 1400, ml], ec: 2.4 _______ Saturday 17 AC3Q 64:F:4:1 CEASED FOLIAR Application: 2022-09-17T16:02:06-0500 # - [X] Complete Week’s GrowDiaries

Hello growers and tokers! 👋 👩‍🌾 🧑‍🌾.🔥💨 There has been much change this week!! First there was a big defoliation done, I cleaned the bottom of the plant. All the growth that wouldn't amount to anything because the light doesn't penetrate that low. Also took off the biggest fan leaves to give more room for light. Then finally switched to 12/12.. After just 36 hours after the defoliation there was a major change. New growth everywhere, colas started stretching upwards fast. It's like she already knows it's flowering time. Great reaction to the defoliation and 12/12 switch. I'm still watering every other day, After the defoliation I watered with only Enzymes 2ml/L to clean the roots a bit then started with the nutrientes again. I dropped the amount of grow nutrients from 3ml/L to 1ml/L and started adding bloom nutrientes 2ml/L for now. I'll slowly be upping the bloom nutrientes and after week 2 I'll no longer add grow nutrients. That's it for this week. Stay tuned to see how she flowers. Stay safe!