3 semana de vegetación y noté una clara deficiencia en las 2 plantas (1 más que en la otra) al principio no sabia lo que podía ser, y varios cultivadores me dieron opciones, busque la composición del alimento que uso y me di cuenta que no tiene calcio y muy poco porcentaje de magnesio, aparte que estoy regando con agua purificada+ósmosis inversa, así que por conclusión es carencia de calcio y magnesio, me recomendaron calmag un alimento rico en los 2 nutrientes así que lo compraré y espero ese sea el problema, no hay estancamiento pero si están creciendo más lento Hoy toco riego con alimento siguiendo la tabla de green house, realicé un riego foliar con acti vera como preventivo de alguna plaga u hongo.

Growing frosty buds and enjoying life. Marks of the previous accident are still observable. But she is growing great smelling flowers.

========27.9 - day 37====== Not looking any better today ,little bit more yellowing and dying foliage. ******** Fed all the plants 🌱 with a mild strength EC 0.7 PH 6.5 *********** ========28.9- day 38======= today I see some perky top leaves on the widow and the banana 🍌 hopefully This is some signs of recovery.... Cleaned off the dead leaves 🍁 And not doing much today ,letting soil dry ... what do you guys think about this ? The strong ec helped bring plants to life or was it the mild feeding that did the magic ? Let me know what you think in the comments below 🙏👊much love to all the awesome growers on this platform for sharing your knowledge and time with noobs like myself 🌱🙌💪💚 =======29.9 - day 39======= Just fed 650ml to all plants with 1.0 ec 6.5 ph , heaven grow and bloom . =======30.9 - day 40======= Plants doing they’re thing , I fed yesterday so nothing today ... Took some photos when hps turned off... Also looking to buy new led grow light the hips electric bills and temperatures are too high ... I’m done with that nightmare 😂🤯 Next diaries will be led 👊 =======1.10 - day 41 ======= Pots are light so all been fed again , this time added TOP MAX into the mix ( 1.5ml per liter) PH 6.3 EC 1.0 Praying leaves on the banana and white widow again =======2.10 - day 42 ====== Drying out and chilling :) Still not so sure I fixed the problem 100% , not exactly what I was hoping for with this grow ... I’m sure this will mess with the final results 🥊 Good chance for learning a thing or two.

.

Eccoci qui... Per problemi di lavoro ho tardato un pò la pubblicazione delle foto, le piccole sono molto in forma ed ho fatto molto bene a puntare su di loro mettendone 2. STRAIN 1 sta sfocando le foglie con un colore viola scuro molto molto interessante, per strain 2 invece il discorso è più lento ma comunque ci sono i primi segni di viola. Grazie a tutti per il supporto, NON VEDO L'ORA DI RACCOGLIERLA 🔥🌲❤️

10 days to go!! Gorilla Zkittlez it’s a great strain with a fruit aroma in the air..bubba are small buds and most of them are creamy.. hope in a good smoke!

Week 6 of growth was another bumpy ride. Temperatures climbed up to 27–30 °C (Celsius), which is definitely on the hot side, and humidity sometimes dropped as low as 40–45%. That combo put some serious stress on the plant. To fix it, I made a simple but effective move: I turned off the exhaust fan. Right away, humidity went up to about 70%, and the plant clearly started looking more comfortable. She seems to be handling these new conditions much better. I did make a mistake this week, though. Wanting to push growth a little too fast, I cranked up the light intensity too quickly, jumping from 300 to nearly 600 PPFD. With humidity already so low, that sudden boost dried the plant out. She definitely felt it, but I caught it in time, adjusted back, and now she’s recovering well. A bit of lost time, but nothing dramatic. The real highlight of the week has to be the Athena nutrients. The pH stayed locked at 6.1 from start to finish, without moving at all. I really appreciate that kind of stability, and I’m excited to see how it will impact the plant’s health and growth over the next few weeks.

31 Aug. I start the third week of my autoflower. The plant is growing well, but I noticed small yellow spots on the leaf tips, which seem like a mild calcium deficiency. This is probably due to the fact that so far I have been giving water with a slightly low pH, around 6, while the ideal range would be 6.5–7. At the beginning, to lower the ppm of the tap water (which was around 211 ppm), I mixed it with demineralized water. This brought the ppm down to about 109–140, making it harder for the plant to absorb the available calcium. Additionally, the soil I am using, Biobizz Light Mix, is lightly fertilized but does not contain enough calcium to correct this small initial deficiency. For this week, I will not add any fertilizers. I simply want to raise the pH to 6.5 and use water with higher ppm, so the plant can better absorb the nutrients already present in the soil. The lamp is positioned about 35–36 cm from the plant and so far there are no burn issues. Temperatures range between 25 and 30 °C depending on the day, while humidity in the room stays between 40–60%. The humidifier is always on because I keep the window open, but the dehumidifier is not being used, as it is not necessary at the moment. I also have a new oscillating fan arriving to replace the current fixed one, and I’m waiting for a regulable extractor (90–145 m³/h), which will allow me to control the air perfectly during winter if needed. This week my main goals are: Increase the water ppm without overdoing it Adjust the pH to 6.5 Keep observing the plant without adding any fertilizers Everything else seems fine and growth is regular. With these corrections, I expect the new leaves to grow healthy and free of spots. ==UPDATE 31/08== To control the calcium deficiency, I will administer an eggshell solution until I reach 300 ppm (I will boil some eggshells and administer the solution in tap water). ==Update 4 Sept== By mistake, I adjusted the pH twice, once to 7 and once to 8, and obviously the plant immediately showed symptoms of incorrect pH. At the next watering, I'll give Biobizz Grow with a little Biobizz Bloom since the plant has entered pre-flowering. I'll adjust everything to a pH of 6-7 and a ppm of 450-500.

En breve a flora

looks like i got all the phenotypes from all the strains 😂

DAY EIGHT It seems to be going well, I think. The temperature and humidity have been right about where I want them; 79 F and 60% plus or minus a few. I added a DIY LED I made from the small bar LEDs I had initially used for germination. Not very pretty but any added wattage is appreciated. This will only be temporary. I also added a bit of coco coir to each cup topped with ocean forest. DAY NINE Plants look healthy, a little droopy but it was after dark period. Gave them some water. No real changes. DAY TEN Plants are looking good. I did not water them today because the soil seemed okay. Humidity and temp are stable. I posted a video to show off my ghetto lighting system. DAY ELEVEN No adverse changes noted. Watered them well after their dark period. Temperature has been averaging about 81 F with 65% humidity. I haven’t changed the setting of the fans, lighting, or humidifier. DAY TWELVE No changes made. I gave them a light watering and lightly compacted soil a bit. I plan on transferring them to 5 gallon pots soon. DAY THIRTEEN I gave them 24 hours of light (not by design) before I transplanted the two girls from their solo cups around 3 o’clock this afternoon. I decided to put them each into 5 gallon fabric pots with a base of Fox Farm Ocean Forest. I gently cut the bottom rim off the solo cup using pruning scissors, then cut straight up the side. I was able to pull the cup apart without disturbing the roots and soil hardly at all. I did not loosen the soil at this time, as I was afraid to touch the roots at all. I had a small spray bottle with spring water and two tbsp of Dynomyco dissolved in it, which I sprayed half the contents onto each of the root balls. I also sprinkled a bit into the soil and mixed. I basically have the bottom third of my 5 gallon fabric pots a pretty even mix of Fox Farm Ocean Forest, vermiculite, perlite, and coco coir. Then the rest was 90%+ FFOF soil. I put a bit of vermiculite on top of the soil around the seedling to give it some support. As far as watering, I gave the soil a bit of spring water when the pot was halfway full, enough to dampen it. I then watered it again once the plant was transferred a pretty good amount, probably 16 ounces or so of spring water with a bit of aloe in it. Now they will sit in the dark for 4 hours, and I will keep the temperature and humidity around 78F and 65% give it take a bit. DAY FOURTEEN They don’t look they are dying; I guess that’s a good sign. They seem okay in their final pots. Temperature slowly dropped to 70 F for a couple hours when I was out earlier. It’s back to its stable 79ish. No adverse findings.

10-8-2024 TOP DRESSING AND WORM TEA..... NOTHING MORE 10-9-2024 22ND DAYS INTO FLOWERING SMELLS CRAZY ON THESE STRAIN GETTING SOME SNOW ON TOP LET'S GO 10-11-2024 DO NOT HAVE ANY PEST BUT JUST IN CASE NATURESGOODGUYS HOOK ME UP WITH LADYBUGS JUST GOT HANGING PATCH ABOUT /WITH SHIPPING 10-14-24 NOTHING TO DO...JUST WATER (PH6.6)AND WATCHING THE PLANTS....... COPY CAT GENETIX STRAINS ARE CRAZY!!!!!!!!

Two low branch broke off due to wind and stem wasnt stong enough for weight of the buds....other than that just a handful or two of defoliation every two day or so....makes trimming ez lol

- Raised cal mag from 3 to 5 because I thought plant was lacking cal mag - Upped nutes but it may have led to nute burn in the next few weeks (see pics) - ~350-400 ppm for half nutes - buds starting to form nicely

The first plant makes 20 gr. , the second one 55 gr. Now in curing with boveda 62%. The plants created a lot of smell during the end of flowering and the drying process.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welcome to my Banana Purple Punch diary. 🍌💜🤪💥🥊💨 In this Diary: Seeds: [420 Fast Buds]from my growmie Tropicannibis_Todd 👊🏻😎 Media: Pro~Mix HP Open Top Grow Bag, Connect. Nutrients: Green Planet Nutrients, 2 Part Dual Fuel starter kit. RealGrowers: Recharge. Diablo nutrients: Ripping. Advanced Nutrients: Flawless Finish. ___________________________ Feeding : Mon 01Apr: 3L Monster K pH'd 6.5 Fri 05Apr: 2L Flawless Finish pH'd 6.5 ___________________________ Wednesday 03-Apr-24 i broke a branch somehow😭 Today Monday 08-Apr-24 is 83 Days from seed or 79 Days from Sprout🌱and the trichomes are just about right. Tomorrow morning the chopping will happen.😁 ___________________________ Thanks for stopping by, likes and comments are appreciated.👊🏻😎 Keep on growin! Keep on tokin!!! 😙💨💨💨💨💨

Week 11 is here early this time, as i did some stacking stuff in photoshop today! Watered the Afghan with 2,3 Liters, ramped up the dosage to final. The smell also now i comfortably classify as "strong". They smell really good! More updates and pics at the end of this week. Im starting to check trichomes, feeling wise i'd give the blueberry 1-2 more weeks, the afghan kush is probably at least 2 more. still lots of clear trichomes on both of them though. given that they were a lot slower than my previos run in everything, i also wouldnt be surprised if we take it to 3+ weeks on both. Sadly, the blueberry is already starting to swell a bit, but all the flowers are still really really small. Hoping for the best, and luckily im not out after maximizing yield, as long as they taste, smell and smoke good! 31.01.25 Added Timelapse. See you next week!

Nothing to see here ;)