Miei cari amici, L'estate incalza e finalmente il tempoi permette di trasformare ad hoc. Secondo i gusti il giardino, la terrazza e i due balconi. Un lavoro duro e intenso con i 25/30 gradi che il NW in questa settima sta proponendo e che spero vivamente durino tanto. Travaso storico e professionale. Vediamo ciò che l'armadio dice. Questo travaso probabilmente ritardera di due o tre settimane il raccolto, ma andava fatto e ne varrà sicuramente la pena. 28/06 My dearest friends, comrades and farm partners. Doc. Cannas is extremely happy to introduce you to the 5 wonders of Expert Seeds. Still my most heartfelt congratulations for their genetics, between photos and videos you have seen what a wonder these little girls are growing. Today they have been introduced to LST, an art halfway between Astana yoga and BSDM. Being their first time there was a need for love and passion. So it was. At the next update farmers

Oh well, not my (our) week 🙄 During the week the Ladys start to look a overwatered and I had no idea how this could happen ... Well, first I tried to rise temperature slightly and had the fans run more often to dry them out a bit quicker, did not work. Than I took them out of the System ... All together worked, at least it looked so ... for 1 day ... Yesterday I found the real reason. The Humidity Controller was broken ... During the night they had around 90% humidity and during the day only 25-30%, way to high and way to low. Replaced the Controller today, hope next week will start smoother 🙂 Beside of this they made huuugggeeee progress, some nearly tribbeld in size and are around 5-6 Nodes now, will top them all next week when they recovered a bit 🙂 Also added some Alfaboost to help them a bit. In the Videos the Misty Gorillas are in the middle 🙂 Thanks for your time, I hope you enjoyed this entry 🙂 See you next week 💚

Hello weed green thumb friends My experience with these girls was fascinating, although as often happens the phenotypes seem to be totally different... there is always one who is born to become your favorite 😜 My favorite girl has grown some WONDERFUL flowers and I am so impressed with her huge resin filled flowers.....mmmmmm I can't wait to get my mouth on them 😋

26.07. Wedding Cake 1# Day 92# Wedding Cake 2# Day 86# Wedding has not grown much again this week, but it is still spreading abnormally, the trunk and branches are getting sickly thick, a huge difference from the last update... I don't think it will go much higher by the time it stretches, which would be ideal. Today it started to rain and it will rain for the next 4-5 days, while there will be storms for 2-3 days, and it will cool down a lot and the next 10 days will be much better weather... today I added another layer of netting to this row and pulled them through, in a few days I will add the third and let them grow through it. I forgot to take a picture of the second one, it will be in the next update..everything is the same with her, constantly hungry and thirsty, from the next feeding I'm going to force her with food, so let's see how much she eats because this is unreal lol Stay High and Keep Growing!!!

This week was real great today is day 103 an these girls are all drying out for 12- 14 days ! Then will be ready for trim and cure! Keep your eyes peeled for next week , will have more photos when we get to trimming! Cheers everybody Happy grows🤙

Hotovo😎

Song of the week : Kranium - Sidung ------- Day 58 - 03.12 - No more vertical grow but I had to do some more Hst on the tallest to fit in the tent. They are all forming buds but only #2 is green like I want to , the 2 others are pale ------- Day 60 - I made a short video to show them how they looks when they are outside from the tent. The #3 have a light burn due to the nights under 150w how. The soil I used was a regular gardening soil from the local store but It's astonishing how heavy this soil is even when It's dry bit when drying the surface becomes hard like rocks ( more than usual ) ------- Day 61 - Due to the rain I did not not them out today , I think it's the second time it happens and I hope it will stop soon because they are clearly too high and whatever the position I choose their is always one leaf touching the HID bulb 😒 I also made a short video to show you the box , I have add an extra 10w white light spot under the canopy and a 7w full spectum bulb under just because I had them at home , it wasn't planned at all.

Starting to drop the night time temps down a but but keeping the total change from day to night with in reason. The stretch is coming to an end and the bud sites are showing a lot of pistils. A lot of frost on the sugar leaves already. Things are looking good so far.

She stretched a little bit more in the last week, everything very healthy.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

SUNSET SHERBET Sponsored by **Zamnesia ** 11/24-30 week 12 Welcome to week 12 of my Sunset Sherbet journey sponsored by Zamnesia. This is the last look for Sunset Sherbet before chop. We are experiencing a very cold snap with nights in the 20f-30f and days only in the 40f. I decided to take advantage of the cold and move her from the tent and put her in the garage in hopes of bringing out some colors. I’m only giving water phd’ to 6.6. Water temp is in the low 50s, light is filtered thru the window. All her trichomes look milky so next weekend she will be chopped. Your likes and comments are appreciated. Thanks for stopping by. Growers love 💚🌿 💫Natrona💫

Day 71 - I watered her with nutrients, PH was at 5.3. Plant looks very healthy. I think i need to get some stucks to support stem. Day 72 - Checked in the morning properly, the plants looks fine checked for hermies couldn't see anything. Day 73 - I checked her today again nothing that can ruin the whole crop. She was watered with nutrients today at PH 5.9. I got new lights today spider farmer sf2000, this looks amazing inside there. We have also done a bit of light Defoliating so the buds have more nutrients. Day 74 - The plant is fine but the temperature is very high after getting new lights its on 34°c I've order a new fan so i can have inlet and outlet to try cool it down. Day 75 - The plant looks a bit sore thats because i should have watered her earlier. I've given the plant 5.5PH water with nutrients. Day 76 - So once again i didn't take a picture today.  I was very busy and didn't have time. I checked the plants as soon as i woke up and they looked well and healthy. Day 77 - Plants looks well would like it o look like its gonna give me a half decent yield but i don't know let's see how it goes. I also watered her today with nutrients and the water Ph was at 5.5.

Cruising right along. I bent over the stalk and had to take a couple of the massive leaves off and it’s back up to 12”. I love my new system and the easy maintenance makes it even better.

Hallo zusammen 🤙. Das wird ein Outdoor und Indoor Grow. Ich werde sie bis Ende August draußen bei mir auf dem Balkon wachsen lassen und die Blüte werde ich dann im Zelt machen.

After the FBA was chopped, the LCC remains alone for at least one more week. You can really see the damages the flies left. A few leafes are turning red. Its time for a bit more color!

It’s day 27 from seed for all the plants in the photo sea of green. Let’s call it the battle of the strains summer 2025. All of the plants are starting to have visible differences. I’m already starting to tell which plants are more sensitive than others.

The tent smells great this week. Very sweet, pineapple citrus. Buds continue to grow with new pistals daily. Lots of crystal showing now too. I didn’t defoliate as much as I’d hoped due to hermie sighting in week 3; but they’re looking good and I’m happy with grow three so far. So much learned, so much progress. Hopefully the results speak for themselves...

These buds are absolutely awesome, very frosty solid dense nugs👍 have been flushing this week as there ready to harvest . Orange pistills cloudy trichomes with some amber's & the clusters have closed up . Will give them 48 hours of darkness before harvest.

Tag 49 - Ende der 7. Woche. In dieser Woche haben die Blüten nochmal ordentlich an Volumen dazu gewonnen und produzieren eine dicke Harzschicht. Auch der Duft der Blüten wurde in dieser Woche intensiver. Da sich die Pflanze nun vollkommen auf die Produktion der Blüten konzentriert, habe ich den Dünger nochmal etwas angepasst/verändert um der Pflanze alle Nährstoffe zu liefern die sie für die Produktion der Blüten benötigt. An Tag 43 wurde die Pflanze gedüngt. Nährstofflösung: 2 ml/l - B-52 Advanced Nutrients. 4 ml/l - pH Perfect Sensi Bloom Part A 4 ml/l - pH Perfect Sensi Bloom Part B 2 ml/l - Bud Candy Advanced Nutrients. 2 ml/l - Big Bud Advanced Nutrients. Davon hat die Pflanze 1l an Tag 43 erhalten.

Day 8-15/04/22 everything looking good been giving her plain water since the begging will start to add some nutrients next watering!!!! - Day 11-18/04/22 gave her some root juice yesterday and looking very good