- BioBizz Light Mix soil - BioBizz Nutrients used according to their schedule - stopped LST

Day 57 she’s beautiful happy loving life. I can’t wait for her to be done. Mhhhhh my Favorite.

Salut amis cultivateurs 🍁 On es enfin en semaine 4 de floraison. On peut voir que la GC et la SB on énormément évoluer et surtout gonfler. Après avoir discuté énormément avec une personne sur le site, j'ai changé ma façon de faire sur les arrosage. Et comment dire que je suis étonné de voir comment elle ont réagi 😱 super bien et pourtant je force je force . Tous les jours je leurs donne 2L chacune et tout les jours et pompe au maximum ce que je leur file . Aucun problème d'humidité, ou carence .. J'ai tout de même effectué une cure de calmag pour éventuellement contré ce fort apport en nutriments. Et regarder par vous même ❤️ MAGNIFIQUE FASTBUDS FAITE MOI CADEAU DE GRAINES ❤️ j'adore vos génétique propose et constante

3/14: Came back from a 4 day vacation to a lot of dry, wilted, dead leaves on Amnesia. Trainwreck is super starved as well. My automatic watering system didn't kick on, and the humidifier ran out of water within 24 hours, so there's that. It may be for the better though, as the PH of the nutrients had risen to 7.5. I tossed that mix, and made another 2 gallons of half strength nutes, molasses and epsom salt. I watered them both to runoff, and now I'll wait for Amnesia to prioritize which leaves she thinks are worth saving, and then I'll defoliate the dead ones. Hopefully trainwreck will just bounce back to green. She needed to be defoliated anyway, so this situation makes it easier for me to find the dead leaves. Edit: Came back to feed the last of the nutes and decided to pluck the crispy goners. I found that pulling the leaf stem downward will make the most dead leaves fall right off, allowing me to test a majority of the leaves and give Amnesia a major haircut. Luckily I left a lot of leaves from prior defoliation, as Amnesia REALLY sucked them dry. 3/15: Amnesia looks no worse for wear than last night. Trainwreck as well. Went ahead and pulled as many dead leaves off Trainwreck as I could. Some leaves were a mix of crispy brown and healthy green, which I think is pretty goofy, so I avoided taking any green fans but removed the crispy ones. The colors of the leaves around the buds are really fun to look at. 3/16: Amnesia is starting to SHED her dead leaves, allowing me to pick the leaves with almost no effort. I tested as many leaves as I could, giving another haircut. I would say this isn't a good thing, but since it's my first grow, I'm rolling with it. As long as Amnesia keeps the buds and its nearest leaves alive, it will slowly grow, albeit stunted. I'm not sure if this condition will cause it to finish sooner, but I'm going to need to watch the trichomes under a microscope every couple of days just in case she's ready for harvest. Trainwreck looks about the same as it did before. No new leaves are ready to be removed there yet. 3/19: Amnesia is still shedding leaves, but also bulking up. Trainwreck is slowly bulking up, and turning purple as well! Going to keep an eye on Amnesia's trichomes. Edit: because amnesia is yellowing so much, and because it's been 5 days, I'm going to feed both plants a quarter strength nutrient mix just in case I've been overfeeding them somehow.

Looking wonderful. Very close to harvesting. 90% of hairs are orange and the trichomes are 80% cloudy 20% clear I did notice some amber here and there but she's got a wee bit longer.

Veg54, riego con 6.2 pH y EC 1.5, drenaje de 2.0 EC y pH 6.3, todo ok. Las plantas ya alcanzaron entre 25~30cm, en 2 días más comenzará la pre floración. Veg56, están casi de 30 centímetros de altura, último día de vegetación, mañana entrarán a pre floración, necesitan una defoliación.

Esta será la última semana donde incorporamos Bio Vega a la dieta de alimentación. La potencia de las luminarias al 75% y regamos cada tres días aproximadamente alternando entre fertilizante y agua.

Not long left for her now I don't think. Buds are fattening up nicely the main cola is rock hard almost. Ridiculously strong pepper smell to her haha if you give a bud a rub a lovely fruity smell comes through though. Gonna do a week of dragon force next week then possibly start flush depending on trichomes. I have even checked them at all yet but will get it done over the weekend.

So this is the start of the fourth week with me. I find this very engulfing. I like spending an hour with them a day and move the little ones out of the bigger fan leaves shadow by tucking the larger ones away giving the little ones a chance to get more light and flourish. I’m noticing more the strain my one girl went through compared to the others, her leaves being smaller than the other girls. I’ve tried to give them the best environment I can and realize I still play a huge role in their life. This is my first grow and I love it. I hope they grow up well and flourish. They were fed last week and had a bit of nutrient burn so this week I gave them just water. I’m not sure how much longer I should veg but I hear in the scrog game the longer you veg the better. Hoping for the best for everyone on GrowDiaries and their grows!

----- WEEK 9 ----- ----- Day 57 ----- Easy day of watching. Just 1 pic today so far. not much reason to go downstairs to take a pic yet. Glookies is turning Purple on sugar and fan leaves now. Harvest is very soon. I'd wager within 7 days now just going to depend on the High I get off tester nugs. ***Update*** I destroyed the trellis and took em out for some pics and vids. Trichs = Tropicana is ready Tuesday, Wedding Cake probably Friday or within that weekend. ----- Day 58 ----- Last couple days. Fed about 30ml of Cal/Mag and 30ml of Molasses. Wanted to just keep the photosynthesis process and the plant in general alive for reveg. Was getting a bit too yellow for my liking. Harvest soon. ----- Day 59 ----- Harvested Tropicanna Glookies today Deleted this week by accident trying to make it a harvest week, so remade it. Will add harvest week once plants are dry and tester has been smoked to give proper info Put some more photos in. Basically the tent is 4 feet wide and the ruler is 12 inches long from bottom of duct tape Really loving the colors of this plant. ------ Day 60 ----- Fed only the Wedding Cake - 5-10ml of Calimagic and 20ml of molasses (NO Liter level marked or PPM or PH, i could cut today its done but im just maturing the trichs) Tropicanna Glookies is a friggin BEAUTIFUL strain. Moved the Wedding Cake in the tent so largest nugs are furthest away from light and dropped light from 1000w to 600w to save electricity Chop on Wedding Cake happens at mostly amber trichs. Prob 5-6 days max for that i'll know when I check trichs tonight and update with photo. ----- Day 61 ----- Wedding Cake is about 5% amber... so prob 6 days or so. Slow day today. ----- Day 62 ----- Feeding is today, going to just use some CaliMagic and Molasses again. Trichs aren't golden yet, so not chop time ... 8-9 weeks is what it is supposed to take, but it's got at least another 5-8+ days until 30-40% amber trichs for Indica couch-lock effect to shine in the Wedding Cake. Tropicanna could still be going right now, but I did not want more than 5-10% amber gold trichs to prevent a couch-lock effect. This has worked very well. On day 64 I will post a smoke report and full harvest/trim details as Saturday/Sunday is trimming. Will have dry weight results of Tropicanna (per plant, and as a whole). Tropicanna being sent to lab for full analysis will post results when they arrive (Usually 2-3 weeks after sending sample via mail) ------ Day 63 ------ Nothing to talk about today, slow day. almost done. *Update* 1 pic, i think it explains everything.

7 Diciembre: Se cambiaron las plantas desde la carpa de vegetación(120x120) a la de floración(150x150) en dónde hay más espacio para deshumidificador, ventiladores, etc. Se sigue regando con 6.2pH y 3.0EC. Pronto se agregará una malla scrog. 10 Diciembre: ya hay claros signos de floración, se colocó una primera capa de malla scrog, el riego Generativo va como se planeó, con pequeños runoff llegando a 7EC, esperando subir hasta 10EC, con ese estrés controlado se mantienen las plantas en una altura óptima evitando que estiren mucho. Hasta el día de ayer, las plantas estaban con 5 disparos(riegos) de 200ml cada uno aproximadamente, hasta obtener runoff, hoy sin embargo, habían 2 plantas con "wilting" lo que indica drybacks excesivos, por lo que hoy se agregaron 2 disparos extra, también llamados P1, y 2 mini riegos de 100 ML en la tarde, P2, para evitar el secado excesivo y el decaimiento de las plantas, las cuales están pidiendo más nutrientes. El VPD se mantiene en 1.2~1.4.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Plantas en sus últimas semanas. Salieron excelentes.

Blüte geht voran. Die Raubmilben Amblyseius (Neoseiulus) cucumeris sind schnell da gewesen 🙏 Ich hoffe, sie halten den Thripsbefall bis zum Ende der Blüte unter Kontrolle. Bisher kann ich noch nicht viel zu den Terps sagen, sehr dezent alles. Zudem haben die Pflanzen die Tage zum ersten Mal einzeln einen Fermakor-Hydrolysat als Blütenbooster in sehr gering konzentrierter Dosis erhalten. Danke geht hier nochmal raus @yan420 Ich verspreche mir durch die direkte Verfügbarkeit der Nährstoffe P und K und den Aminosäuren einen metabolismischen Boost. Bin auf die Endergebnisse wie immer sehr gespannt.

Dia 134 y semana 6 de floración,ultimo dia de vida de las plantas. Este año ha sido un poco raro las plantas han crecido como monstruos tuvieron un crecimiento espectacular un tallo muy grande y junto con sus ramas flexibles y muy resistentes. Pero al llegar el engorde de floración no se porque les falto el empujon final para hinchar el cogollo, tiene muy buena pinta , mucha resina y muy fuerte buen olor pero me esperaba mas en la fase final. Cortamos ya que tambien habian orugas y no terminaba de eliminarlas por completo, la semana que viene era entera de lluvias así que decidi cortar un poco mas temprano para poder prevenir cualquier mal mayor, hay mucha gente que le gusta cortar antes de tiempo ya que el efecto es muy diferente, este año probaremos que tal.

Day 27 of 12/12 Day 16 Flower Ppfd 950-1050. S44-Ionboard Ppfd 650. S11-Ionboard lightbars EC 2.0 PH 6.0 I finally caught this diary up to the current date. I apologize for my previous entries that were all done last night and kind of vague and all over the place. Today I'm doing a mild defoliation to open up the canopy and get some life pumped into these lower bud sites. She's still stretching some which I'm all for at this point although I have a few colas reaching for the sky that need dialed back a little. I'll most likely just LST them to the net if I can find spots to pull from. Defoliation is a nightmare currently. I need some kind of mission impossible harness to hang above the canopy and save my lower back lol. I ordered and installed the s11 Ionbeam led bars from AC Infinity. I currently have them in my lower canopy just below my scrog net to help her along a bit. The first night I installed them they didn't sync up with my controller 69 and she had a night light for 12 hours. Pisses me off because I should have peeked in to make sure it shut off with the main board. So later on today I'll play around with the setting between the 2 controllers and get this UIS set right. This is a prime example of jumping the gun and letting the excitement of new gear get me doing shit hastily. These bars are weak and have to be placed within inches of the canopy and then will only cover a small area efficiently. I will most likely remove em from the lower canopy and suspend them over the colas on the edges. Not sure what I was expecting from 11" light bars. Maybe I'll add them to my seedling/clone racks.

I would be lying if I knew how far her flower phase come, this couple weeks been absence only gave me results, and left me with a catalogue nightmare, but thank goodness my previous posts are dated, so it gives a rough idea, but genes differ within strains, some strain grow vigorously at certain stages, and I think this baby hit her phase. She will be my best tree when it comes bud quantity, when it comes to potency, I will have settle with what I encounter and except the outcome.

Moin an alle der licht plan ist seit dieser Woche auf 12/12 gibt nicht viel zu sagen habe sonntag den 12.06.22 gegossen und dann bis donnerstag den 16.06.22 einfach nix gemacht leider hätte schon mittwochs giessen können. Nach dem giessen am donnerstag habe ich die Ladys nochmal von überschüssigem Material (Seiten Äste und Fächer Blätter) die in die Mitte der Pflanze ragen zu entfernen. Stretch läuft gut sind jetzt knapp 11 cm in 7 Tagen hoffe da kommt noch ein bisschen was wovon ich aber aus gehe so irgendwas zwischen 15 und 30 cm bis zum Ende des stretches noch oben drauf währen schon gut denke ich mal hab ja nicht allzu viel Ahnung vom Main lining is jetzt der 2. Versuch an der Technik (hab gemerkt das es wohl schlauer ist bei der Technik nur eine Pflanze im Zelt zu haben da das die licht Verteilung auf die einzelnen Äste erleichtert und somit alles an Ästen gleichmäßiger beleuchtet wird was dem Wachstum der Äste in Sachen Einheitlichkeit wohl mehr zu Gute kommt) euch noch ne schöne rest woche......