More like HST instead of LST. This week was all about low stress training or lst. But as it was for the very first time I would say it was more of a high stress trainig (for me of course). First ties I started already the previous week but this week was hard tie downs on pretty much every branch. Since I messed up the first week and put my lights on almost max power the plants didnt strech out for light and are pretty low and bushy. Thats why the space is really tight and lst is just the thing to do. I used a metal string since I think its better because I can adjust it more and all tension is not on the string. For the past 2 weeks or so I noticed some weird spots on one leaf. This week it went on 3 more leafs and on one more plant. After some research I found out thats a serious Calcium defficiency. Since I’m not from US I didn’t have the same things as in videos I watched. So I didnt get the regular cal mag. But instead some from local store and I must say it was bad choice. Way too less calcium. Ordered a proper one and hopefully it will come in the next fee days. Another interesting thing happened. At the beginning I was cinsidering toping. But after researching more decided no to go with it on the first grow. As the lack of calcium appeared I went full blast with feeding. And it appears thats I was underfeeding, since I thought that my coco was too wet. Guess what happened when I went from once in 3days to twice per day. Thats right. The growth was massive ! One of the metal strings somehow got stuck in the plant and when this acceleration happened it simply snaped itself of 😳 I must say I was a bit sad at the moment, but then I figured it’s some form of self-toping. Not that I wanted it to happen on my strongest plant. But will see what will happen out of it. Alltogether this was a nice week full of wide strech and a lot of new knowledge!

Girls are growing strong another 1500 ppm 3.0 ec feed yesterday. Im not feeling well today so just a quick update. Thanks for all the looks and likes. It's a great growmunity here.

Es ist schon ein ganzes Weilchen her aber ich erfreue mich immer wieder an diesen blühprächtigen Pflanzen😍

Растениям от IZI осталось дней 7. Анубису дней 10-14, CloudWalker'у от двух недель, максимум 3, фенотип сативный + поздний зацвет (почти на неделю позже) Как тут внести новые удобрения? Добавить его в базу. Хоп-хей спамеры, кто зарабатывает хуй знает сколько в час? Общий вид не возможен - сломалась камера, только макро.

A

Looking and smelling beautiful, she is getting close to the finish..

Checkout my Instagram @smallbudz to see the Small budget grow setup for indoor use, low watt, low heat, low noise, step by step. 01/11/2019 - Started using BioBizz Alg-a-Mic on spray, using it once per day at lights off, 1 or 2 puffs to create a light mist, only on non feed days, I mixed 0.1ml on 100ml water 6.4 PH. 03/11/2019 - Gave her 250ml plain water with 6.4PH 05/11/2019 - Gave her 0,15ml of each BioBizz Grow, Max and Bloom on 300ml water 6 PH didn't manage to get it higher to 6.3 at least. 06/11/2019 - She started to show some pistols, probably entering pre-flower. 07/11/2019 - Soil was to dry due to 2930c temps gave her 0,500ml of 6.5PH water with 0,25ml Grow, 0,25ml Max, and 0,45ml Bloom BioBizz.

🍊👨‍🚀🧡 Depuis quelques temps je ne vois pas beaucoup ma culture car j'ai déménagé et qu'elle ne se trouve plus chez moi. Je passe la voir uniquement pour l'arroser, environ une fois par semaine. J'ajoute du Bioenhancer une fois sur 2. Certaines de mes Lemon Orange ont poussé plus que je ne m'y attendais ! La semaine prochaine, je vais retirer les sceaux que j'ai mis en dessous pour essayer de les remettre à la même hauteur que les autres. Sinon les plantes m'ont l'air d'être en bonne forme. Pourvu que ça dure ! 🧡👋 Intensité de la FC3000: 90% Ventilation : Extracteur mars hydro 6 pouces avec filtre à charbon puissance : 4/10 (24h/24h) + 3 ventilateurs à l'intérieur ( ON 8/24h). ils s'activent à un horaire différent. 🌟Venez me voir sur Instagram 🌠 https://www.instagram.com/hou_stone420/ 😘🍊🧡

Finally back to it!

Started to worry about space in my tent, so flip time, especially given the fact I still have other strains that need to fit in here, only decided to document the Dos Si Dos as my other strains are either from cuttings and are very secret lol or they are seeds I received as samples.

This is what's left in the tent, the gelato is comin down tonight and maybe the 2 smaller lemon aks maybe tmm. The cream cookies and other lemon AK will come down soon too and then I can get some pics. The pineapple and blue dream get 1 more flush

Just perfect harvest thanks to Platinium for their great fertilizer and to barney's farm for this strain that I recommend a just incredible taste🤤🤤🙏

👉Alrighty Then👈 👉Cherry Punch F1👈 We had some real nice growth this , had to do a little leaf management👈 We be in full flower mode ..... Everything is looking good 👍Having issues due to using my well water , it's hard so I'm having slight issues but I am on it ..... So ive up'd the nutrients to accommodate pre flower ..... Soil by Promix Nutrients by Cronks Lights by MarsHydro.ca FC4800 X UR45 X Adlite Deep Red And Blue Tent 4x4x6.5 & Equipment by MarsHydro.ca High reflectivity inner mylar Thick Oxford fabric Smooth heavy duty zipper Sturdy metal frame Zipper blackout cloth Good anti light leakage performance The 6in Inline kicks ass moves alot of air ●Blue Light Effect: Blue light shortens internodes, resulting in shorter, stronger plants, ideal for supporting fruit development later. During vegetative growth, blue light promotes lateral branch development effectively. Well this should be fun 🙃 Thanks to all my growmies out there for stopping by its much appreciated 👈 👉Happy Growing👈

Week 4 (8-7 to 14-7) 8-7 Temps: 20.7 to 26.1 degrees Humidity: 49% to 57% 9-7 Temps: 22.2 to 27.5 degrees Humidity: 52% to 65% 10-7 Temps: 22.8 to 27.4 degrees Humidity: 51% to 65% 11-7 Temps: 22.2 to 25.9 degrees Humidity: 47% to 56% 12-7 Temps: 19.9 to 25.9 degrees Humidity: 47% to 62% Watering: Both 1000 ml. 13-7 Temps: 19.9 to 25.1 degrees Humidity: 47% to 60% 14-7 Temps: 19.9 to 26.6 degrees Humidity: 45% to 60% Watering: Both 1000 ml. Today i topped both plants! Light set to 60% strength with a distance of 60 cm.

Just started them all on the Green Buzz Liquid Organics and will run it the rest of the way. I'm not going to add much to the line just some calmag, enzymes maybe terpinator I have to experiment with it a little bit. The first feed went extremely well the plants instantly reacted within an hour after the feed especially the next day. The additives in this line barely raised PPM at all which I liked. It also doesnt stink like other liquid organics I've used. These are doing good the big one seems to be flowering faster than the first run from seed with this cut. The second one is still completely untrained and the 3rd one is the weakling. Day 21 of bloom. Added in a shot of the feed chart I'm using from G Buzz, white board is where I record the run off values especially during the flush👍

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.