2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Plant packing on the weight, trichomes starting to form. Some fan leaves have dried up and fallen off, bringing new light to the bud sites! Little splash of big bud added, to try keep the weight on.

Another solid week in the books, and the last one UKBS 2 will spend in the tent. Today I intended to start hardening the selected plants that will go outside, but when watering UKBS 2 I found it was growing roots out the bottom of the pot and into it's drain tray. So, I had to replant it into the 15gal and will be taking it in and out of the back door until the weather's nice enough overnight to just leave it out. The Carl plant I had taken out to harden was very pissed off with me for doing so, and perked up near-immediately when I took it back in. It's too bad it doesn't like the outside as it's going to be spending a good amount of time there. I treated with diatomaceous earth in an attempt to not bring any unwanted insects into the tent. Both UKBS 2 and Carl 2 will be going in and out every day for approximately the next month while temperatures improve. Inside the tent, UKBS 1 seems very happy to finally have some light. UKBS 2 was very large and encroaching inside there, and blocked nearly all of its light. More updates will come throughout the week, as always. -4/17/2021 - Noticed a small hole at the main stem near the top node on UKBS 2. Dusted with diatomaceous a little bit just in case, will keep a very close eye on it. Hoping it's not a caterpillar or stem borer, and just a breakage. -4/20/2021 - Happy 4/20! UKBS 2 is loving the window spot, and life is good. Thanks for swingin by.

Daily breakthrough of foliage and correction of sprouts in order to maintain symmetry of their growth. I create more comfortable conditions for a lagging sprout.

The beginning of the full flowering phase But there are some species that have not yet bloomed. GORILLA ZKITTLEZ (Week 0–>1) 4 plants SUPERHERO (Week 0->1) 4 plants PURPLE PUNCH COOKIE (W0) 2 plants WATERMELON ZKITTLEZ (W0) 1 plants CAKES N CREAM (W0) 1 plants

Day 20 since time change to 12 / 12h. Hey everyone ☺️. This week she continued to develop very well 🤗. It absorbs the nutrients from Green Buzz Liquids very well. I am very thrilled . otherwise there is nothing more to report this week 🙈. I wish you a nice week, stay healthy 🙏🏻 and let it grow 🍀 You can buy this Strain at https://thecaliconnection.com/original-sour-diesel.html You can buy this Nutrients at https://greenbuzzliquids.com/ Type: Original Sour Diesel ( Clone ) ☝️🏼 Genetics: Fem seeds- Original Sour Diesel to Original Sour Diesel BX3 RVSD Male Reg seeds- Original Sour Diesel x Original Sour Diesel BX3 Male 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green Buzz Liquids : Organic Grow Liquid Organic Bloom Liquid Organic more PK More Roots Fast Buds Humic Acid Plus Growzyme Big Fruits Clean Fruits Cal / Mag Organic Ph - Pulver ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 - 0.4 EC. Add Cal / Mag 2 ml per l water every 2 waterings . Ph with Organic Ph - Pulver to 5.8 .

Die letzten Tage … langsam wird’s Bernstein

Strong strong smell, what an amazing smell, I'm enjoying an amazing strong amnesia haze clasic smell right now, cannot believe this lady is gonna be ready in around 65 days, let's see how she keeps developing in the further days of this 4th week of flower 😋😍👨‍🌾

The end of my Eternity Cup! Very happy with how she turned out. Got a little over 2 ounces of all high quality bud. Zero larf. I waited to do my final post, after she dried as well. I taste the broccoli but I like the funk it has and boy does she hit! Love me a Indica. Thank you promix for always supporting me! you need coco or soil, they have it all. Like Legit! this is the coco I used. https://www.pthorticulture.com/en-us/products/pro-mix-ccx-agtiv-reach Again Thank you Plagron and Zamnesia for another fun ass contest! I was scared AF of this strain, simply b/c I dispise actual Broccoli. But this is very nice! Thank you all for you support and help. much love 😻

Week four marks the true beginning of the flowering phase. Both Pink Rosay ladies have shifted gears and are now growing explosively. The first pistils are clearly visible across both phenotypes, and they look fresh, vibrant, and full of energy. It’s always a beautiful moment when that transition becomes undeniable. The difference between the two phenos is becoming more pronounced. The left plant is stretching much more vigorously, while her sister on the right remains more compact and reserved in her growth. Running two different expressions in the same space always comes with a bit of uncertainty — especially without clones — but that’s part of the adventure. Let’s see how it plays out. For now, I’m deliberately avoiding any defoliation. I don’t want to encourage additional stretch, as they’re already growing fast enough as it is. Sometimes doing less is the better strategy, especially in a limited vertical space like this. At this stage, everything looks healthy and well-balanced. The structure is forming, the flowering sites are multiplying, and the energy in the tent is clearly shifting. The real show is about to begin 🌸

Day 50 - Starting week 8 and gave her a feeding of nutes today. I did remove a couple more fan leaves but she is really think and don’t think I’ll be able to get her thinned out enough and not stress her out. So for now I’m just tucking the leaves as best I can. Day 51 - Did a decent defoliating today but still not enough and don’t want to do too much to stress her out so tucked as much as I could as well. Not really seeing a lot of frost yet but the buds are building up beautifully. Day 52 - Not a whole lot going on right now but again, I removed a few more leaves and tucked the rest again. Plan on giving her some water tomorrow or Thursday. Day 54 - Gave the girl a gallon of water with 7ML of cal-mag today. Didn’t do any defoliating today, just watered her and tucked some more leaves. I’m gonna be out if town for a week so won’t have any updates until next Sunday so hopefully the wife doesn’t kill her while I’m gone.

Greetings to any helpful advice

Diese Woche sind die zwei Damen in ein neues Zuhause umgezogen. Da die anderen Damen leider keine Automatic's sind und ab Montag in die Blüte gehen, habe ich mich entschlossen aufgrund der Lichtverhältnisse ihnen ein neues Zuhause in einem 1,20m x 1,20m x 2,00m Growzelt zu spendieren 😀 mehr folgt...

1st March Mon - So it’s been 3 weeks since I planted her and coming into the 2nd week of veg. I’m happy to say she’s doing well we have now got 2 more leaves so she’s growing well. 2nd March Tue - Updated grow diaries today put out a question on her leaves so will update what I find out. Think I will start giving nutes in a few days but not too sure how to go about that? Will record what I find out, any comments appreciated!!! 3rd March Wed - Nothing new to update today just letting soul dry a little before I add nutes tomorrow. 4th March Thur - First day on nutrients!! Got some fresh ph balanced water at 6.5. Added 4 drops each of Dutch pro soil A+B and 3-4 drops of cal mag. This was added to 2 pints of water gave enough to the plant for it to be damp. Very excited to be moving on with the grow!! 5th March Fri (day 14 since sprout) Very excited to see the plant is responding well to nutrients the leaves are getting much bigger looking forward to see what she has to offer in the 3rd weed of veg. ....disaster struck when I was lowering the light it completely broke off and squashed the plant for no longer than 2 seconds. Only break I can see is on one of the lower leaves but hopefully it’ll bounce back! 6th March Sat - Very very disheartened today! :( but I can only try and see what happens! So I had to take a few hours away after the incident happened because I was ready to go hulksmash on the whole grow tent 😂 but with some advice from a lot of growers on here I’ve got a little motivation to continue! So I’ve tied the plant up with a stick and some thread I’ve very loosely tied it to the stalk so she straightens out. Gave her a little more water with some nutes so hopefully aid her. Will keep everything updated on how she grows with the incident in mind. Any advice at all would be very much appreciated! 7th March Sun - So we’re at the end of “week 2” after the incident I think my girls doing well. I’ve put soil upto her bottom leaves to give her more support as advised!! Gave her some water with nutes hope she picks back up this week. Excited to see how she transforms and grows!! Happy weekend guys!

Como estáis familia? Yo flipando y es que todo lo que provenga de cheese me encanta, farm cheese del banco genofarm, repleta de polen,parece los Pirineos en pleno enero cuando nieva. Es una cepa increíble el problema que le veo es su olor penetrante, apesta enserio es muy fuerte su olor, seguimos alimentándola con advanced nutrients quitamos todos los adictivos menos overdrive y la base sigue dando caña y si no mirar su color. Todo este proyecto e de agradecerlo a mars hydro que sin ellos no es posible este proyecto, enserio este led es bomba. Ph controlado , humedad por debajo de 50% temperatura entorno a 27 algo alta pero no hay problema alguno. Muchas gracias a todo el equipo de mars hydro de nuevo, agrobeta que aunque no participen los productos en el diario si van hacerlo en un futuro, y gracias a vosotros que sin grow diaries y sin todos vosotros usuarios , tampoco es posible, así que gracias, muy buenos humos 💨💨💨.

Am Donnerstag wird geerntet :) riecht super lecker!

Feed log; D:30 - 3.5L - [pH 6.8] Si[0.5ml] ,Vega[5ml], Flores[0],Ca[0.3g/L/]Mg[0.1g/L] INPUT; [1.1 EC] OUTPUT; 3.6L pH 6.0 [2.0EC] previous irrigation: D:17 This phenotype decided it wanted to be stout; as of now the plant is [40] cm tall and growing! 🌱 I’m keeping the PAR levels at 550 for now and forward..

Es gab in dieser Woche keine Probleme und alles ist nach Plan verlaufen. Ich bin sehr zufrieden mit den Ergebnissen

Seeds from Humboldt Seed Company germinated faster, but Green House Seed Co. plants have been growing bigger so far...