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💎 Black Diamond Auto by Zamnesia – Pheno A Week 8 | She's Everywhere Welcome back to another chapter of the 8×8 Adventure! And this week, I have to admit something... I have absolutely no idea where this girl is going. And I love it. 😂💚 Black Diamond Pheno A has officially reached the point where I stop trying to predict what she's going to become and simply enjoy watching her do it. Every week I think I've figured out her structure. Then she does something else. This week? She's building main-cola-sized flowers everywhere. Not just one dominant central cola. Not a huge top surrounded by little side flowers. I'm talking about substantial flowering structures developing all the way down the plant, with some of the lower flowers already starting to resemble little baseballs. And the craziest part? There is almost no popcorn. She's simply turning branch after branch into proper flowering sites. This Black Diamond is something special. 🌱 The 12/12 From Seed Adventure For anyone discovering this diary for the first time, welcome to the 8×8 Adventure. Every plant in this project is being grown under a 12 hours on / 12 hours off light schedule from seed. There is no traditional vegetative period. No extended growth phase. No special treatment for individual phenotypes. Every plant receives the same environment, the same lighting strategy, the same feeding philosophy and the same general care. The genetics are the variable. And that's exactly what makes this experiment so fascinating. When twelve plants are given essentially the same conditions and one of them decides to become a completely different creature... that's the phenotype talking. And Black Diamond Pheno A certainly has something to say. 💎 She's Building Colas Everywhere Last week I described this plant as tall, elegant and balanced. This week? I'm starting to think "balanced" isn't quite enough anymore. At 169 cm, she has developed an incredible vertical structure with flowering sites distributed throughout the plant. But what really catches my attention is the consistency of flower development. Normally, when looking at a plant this size, you expect the hierarchy to be obvious: One main cola. A few strong secondary branches. Then progressively smaller flowers as you move down the plant. Black Diamond Pheno A seems to have decided that hierarchy is optional. Her central cola is still the largest and most obvious flower. Of course it is. But the secondary branches are not behaving like secondary flowers anymore. They're becoming colas in their own right. And even further down the plant, I'm seeing flowers developing with enough volume that calling them "lower buds" almost feels unfair. They're becoming little towers. Some are already approaching baseball-sized proportions. And there is remarkably little popcorn hiding underneath. That is what has me so excited. 🍃 Still Untouched Perhaps the most interesting part of this whole story is what hasn't happened. I haven't topped her. I haven't HST'd her. I haven't performed aggressive defoliation. I haven't stripped the interior. I haven't tried to force her into a particular shape. The only intervention has been the occasional leaf tuck when a large fan leaf was blocking a developing flower. That's it. And now I'm looking at this plant thinking... Maybe she never needed me to shape her in the first place. 😂 This is one of those grows that reminds you that sometimes genetics already have a plan. Our job isn't always to create the structure. Sometimes it's simply to provide the environment and get out of the way. ☀️ She's Eating Every Photon She Can Find And this girl certainly isn't wasting the light. Top canopy? She's using it. Inner canopy? She's using it. Lower canopy? Apparently she wants that too. And now that the Lumiflora under-canopy lighting has joined the party, I'm extremely curious to see how far this phenotype can push flower development throughout the entire vertical structure. The whole idea behind the under-canopy lighting was to give those normally shaded areas access to additional usable light. And Black Diamond Pheno A seems to have plenty of flowering real estate available to take advantage of it. She's capturing photons from above. She's capturing photons from between the branches. She's capturing photons from underneath. At this point I'm almost convinced she's going around the room looking for the last photon that hasn't been claimed yet. 😂💚 💡 Lumiflora Under-Canopy Lighting This is now becoming a particularly interesting part of the experiment. The top of the plant is obviously receiving plenty of light from the main fixtures. But look at the structure of this girl. There are flowers developing everywhere. That makes light distribution throughout the canopy increasingly important. The under-canopy lighting isn't intended to replace the primary fixtures. It's simply another layer of light reaching areas that would naturally receive less. And this is exactly the kind of plant where I want to observe the result. Will the lower flowers continue developing with impressive density? Will the middle of the plant continue filling out? Will the overall flower distribution become more uniform? We're going to find out. I'm not expecting miracles. I'm expecting observation. And that's one of the reasons this project is so much fun. 🌸 Flower Development Is Getting Serious The difference from last week is becoming very obvious. The flowers are no longer simply forming. They're building structure. Calyxes are stacking. Pistils are filling the flowers. Sugar leaves are becoming increasingly covered in visible resin. And the individual flowers are beginning to gain the shape and volume that tells you the real bulking phase is approaching. The central cola is beautiful. But honestly? I'm almost more interested in everything around it. Because when you look down the branches, you keep finding another flower. And another. And another. There is no obvious point where the plant suddenly says: "Okay, that's enough. Everything below this is popcorn." Instead, she just keeps producing. Cola after cola after cola. ❄️ The Frost Is Coming Along for the Ride Another thing becoming increasingly obvious in this week's photographs is the resin coverage. The sugar leaves are carrying a beautiful layer of visible trichomes, and the flowers themselves are becoming increasingly frosty. The close-up photographs really show it. We're still not at the final destination. There's plenty of flower maturation and swelling ahead. But Black Diamond is already giving us a preview of what she's capable of. And I have a feeling that once the flowers really start packing on weight, the visual difference could become spectacular. 💧 Feeding This Week The feeding program continues to support the increasingly demanding flowering phase. This week's recorded recipe: Plagron Terra Bloom — 2.2 ml/L Plagron Power Buds — 1 ml/L Plagron Green Sensation — 1 ml/L Plagron Sugar Royal — 1 ml/L Plagron pH Plus — 0.03 ml/L Plagron Lemon Kick — 0.03 ml/L The nutrient solution is being maintained around pH 6.0, with the recorded EC/TDS reading at approximately 2.0 mS/cm. Watering volume is currently around 1.3 L per plant per 24 hours. And the plant is responding beautifully. No obvious signs of nutritional stress. No dramatic deficiencies. No major environmental complaints. Just growth. Flower production. And more flower production. 🌡️ Environmental Conditions The environment remains stable and consistent, which is exactly what I want during this stage. Current conditions: 🌸 Flowering: Week 8 📏 Height: 169 cm 💡 Light schedule: 12/12 🌡️ Day temperature: 29°C 🌙 Night temperature: 25°C 💧 Relative humidity: 60% pH: 6.0 ⚡ EC: ~2.0 mS/cm 🌱 Substrate temperature: 21°C 💧 Nutrient solution temperature: 21°C 🌬️ CO₂: 639 ppm 💦 Watering: ~1.3 L/plant/24h The goal remains simple: Keep the environment stable and let the plant perform. 📸 This Week's Gallery This week's photographs might be some of my favourites of this phenotype so far. The full-plant images show just how impressive her structure has become. The close-ups tell an even better story. Everywhere you look there is another flowering site. The central cola is developing beautifully, but then you move your eyes down the plant and suddenly discover another substantial flower. And another. And another. Some of those lower flowers are already surprisingly large. The sugar leaves are carrying a beautiful layer of frost. The pistils are still predominantly fresh and white, telling us that there is plenty of development still ahead. And the most remarkable thing? This plant still hasn't been defoliated. She's lush. She's green. She's full. She's flowering everywhere. And somehow she's making it work. 🔮 Looking Ahead This is officially the part of the grow where I'm going to stop making predictions. 😂 Because every time I think I know what Black Diamond Pheno A is going to do... She proves me wrong. What I can say is that the next weeks should be fascinating. The flowers should continue swelling. The calyxes should become more pronounced. Resin production should increase. And those numerous secondary flowering structures have the potential to become some seriously impressive flowers. I'm particularly interested in watching those lower and inner flowers. Because if they continue developing at anything close to the rate we're seeing now... this plant could become an absolute monster from top to bottom. And that's without me forcing her to do it. 💎 Let Her Shine This phenotype has taught me something throughout this run. Sometimes you don't need to push a plant. You don't need to manipulate every branch. You don't need to remove every leaf. You don't need to create the structure yourself. Sometimes... you just need to give the genetics the opportunity to show you what they can do. Black Diamond Pheno A has been given that opportunity. And she's taking it. She's capturing light from the top. She's using the inner canopy. She's reaching into the lower canopy. She's taking advantage of the under-canopy lighting. She's building flowers everywhere. She's filling spaces where I expected to find popcorn. And she's doing all of this while simply being allowed to grow. At this point, I'm not trying to tell her what she should become. I'm just watching. I'm learning. And I'm enjoying the show. Because honestly... I have no idea where this girl is going. But wherever she's going... I absolutely love the direction. 💎🌱 💚 Thank You A huge thank you to everyone following another chapter of the 8×8 Adventure. Thank you to GrowDiaries for providing a place where growers can document, compare, learn and inspire one another. Thank you to Zamnesia for the Black Diamond genetics that gave us this fascinating phenotype to follow. Thank you to Plagron for the nutrition supporting the plants throughout the run. Thank you to Future of Grow LED for providing the main lighting driving this project. Thank you to Lumiflora for joining the adventure and giving us the opportunity to explore what additional under-canopy lighting can contribute to a plant with this kind of structure. And thank you to TrolMaster for helping maintain the stable environment behind the scenes. And finally, thank you to everyone reading, commenting, asking questions and following these plants week after week. Every phenotype in the 8×8 Adventure has its own personality. But Black Diamond Pheno A? She's becoming increasingly difficult to ignore. She's not making one main cola. She's making a whole collection of them. And I can't wait to see what happens next. Growers Love. 💚🌱💎
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End week 3 plants still looking healthy. Removed couple fan leaves blocking bud sites. Temps getting hot outside in spring so tent temps have gone up but still in range to thrive. Humidity is mostly the easiest to control
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Buenas noches familia, sorpresa, jueves noche por aquí , actualizamos las power plant xL. Es la última semana antes de nuestra cosecha, el tiempo se nos echó encima y las Flores ya están echas, se preparó un lavado de raíces y tijeretazo,para sucesivamente colgarlas.( PRIMERA VEZ que se me echa el tiempo encima con la floración, jurao) Me habría gustado darles algo de estrés hídrico, pero lo veremos con las lemon kush y las northern light xL. -power plant xL es una cepa con predominancia sativa, con una corta floración,es un ejemplar fácil de cultivar, fuerte, y vigoroso. Estos ejemplares se cultivaron en 7L en sustrato light MIX de plagrOn, controlando en todo momento el PH de nuestras plantas, y dándoles de comer una gama advanced nutrients bastante básica. -PROS: facil cultivar, flores llenas de resina , flores compactas, ramas laterales largas. -CONTRAS: hay que tutorar por el peso de la flor, floración demasiado rápida a mi gusto (no te das ni cuenta). *Aquí ya me despido hasta la cosecha familia.
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5/12/2024 happy Mother’s Day to all the ladies and buds 5/15 super frosty sugar leaves. I can visually see them standing, that they are so tall 5/18 end of week 4
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6/9 I can't believe it but I had faith. The transplant yesterday LOOKED like it was fucked. I cleaned it up though and left it outside. This morning it was standing straight up. Both transplants were! I put tarps up to block the wind tunnel between buildings. It's still overcast and rainy. Sun isn't supposed to come out for a couple poke days. Chance of thubder storms tomorrow but I'm going to try to get everyone in their forever homes today. Or at least most of them. I plan to light dep a few right in the 3 gallon. I'm sure I'll be updating this after I get some work done. UPDATE: I WENT OVER AND GOT THREE MORE TRANSPLANTED. THAT MEANS FIVE ARE OUTSIDE IN THE CAGE. TWO 10TH PLANET, 2 BLUEBERRY CHEESE AND A LITTLE PURPLE PUNCH. THE 3 GALLON POTS WERE RELATIVELY HEAVY SO I PICKED THE LIGHTER ONES TO TRANSPLANT. I LEFT THE ORHER TWO 10TH'S IN THE GARAGE WITH THE DOOR OPEN WITH THE REMAINING PLANTS. I ALSO ADDED WIND BREAKS AND SUPPORTS TO EVERYTHING. I WANTED TO GET EVERUTHING DONE TODAY BUT THERE MAY BE A THUNDER STORM SO IF I DONT GET IT DONE ITS NOT A BIG DEAL AND ILL FINISH TOMORROW. TRANSPLANTS WENT SMOOTH. I THINK THEY'LL GO EVEN SMOOTHER IF THEY DRY OUT A BIT. STILL HAVE THE 50 AND 40'S TO FILL. Took a bunch of videos but the don't want to load. I'll have to try tomorrow or later tonight. UPDATE: I TOOK TONS OF PICTURES AND VIDEOS BUT THEY DONT WANT TO SEEM TO UPLOAD. IVE GOT SIX IN THERE FINAL HOMES OUTDOORS 20s and 30 gallon smart pots. Still have massive plants but I'm going to out those in the 50 and the 40. I'll finish this shit up tomorrow and get all the girls in their forever homes and if I don't have room for some I was planning on doing a light dep anyway so that will work out good. I learned a lot and transplanting went a lot smoother todsy than before. I feel really proud of myself. The last Transplant I did break a few leaves off that I didn't notice but whatever. I used tomato cages for suppirt on two and bamboo poles and string on the others. I'll add the vertical trellis as they grow. I'm proud of myself. 6/10 I got a lot done yesterday. Six are outside already. Two are in 20s in the garage. I'm planning on transplanting the rest today or tomorrow. I'll update later. Accidently uploaded lots of stuff on the wrong week. UPDATE: I TRANSPLANTED THAT SUPER TALL PURPLE PUNCH THAT I DIDNT TOP OR HST AT ALL INTO A 40 GALLON TUB. ROOTS WERE DRY AND THIS TIME IT ACTUALLY LOOKED LIKE THEY FELL AWAY. THE PLANT HOWEVER STAYED STANDINGVUP FINE. I THINK THIS PHENO IS GOING TO BE A TOUGH ONE. LOOKS GOOD LIKE AN HOUR LATER. I HAD THE 50 READY TO TRANSPLANT THIS MONSTER BLUEBERRY CHEESE I FIMed BUT THE POTS WERE SUPER HEAVY AND I FIGURED I NEEDED TO SLOW DOWN AND THINK. WE HAVE THUNDER STORMS COMING TONIGHT. TOMORROW IS GOOD. I MAY TRY TO FINISH UP TONIGHT BUT WE SHALL SEE. THE GOOD THING WITH DOING IT A FEW AT A TIME IS THAT IF SOMETHING HAPPENS OUTSIDE IVE STILL GOT A CROP INSIDE LOL. WHAT IM PLANNING TO DO IS EITHER LIGHT DEP THAT BIG BLUEBERRY CHEESE OR PUT IT IN THE FIFTY BUT IM PLANNING TO LIGHT 3 RIGHT IN THEIR 3 GALLON CONTAINERS. HAVENT DECUDED FOR SURE BUT IM THINKING THATS WHATS GOING TO HAPPEN.......of course videos won't upload and the ones that do are old videos. Damn. I need to sharpen my computer skills....and my growing skills. Especially transplanting. I suck at transplanting. I decided not to out all my eggs in one basket and left the 10th planet amd Blue berry cheese in the 20s in the garage. I pulled them out for light further but I'm ousting them back a little tonight. That way I'll have a back up if something catastrophic happens. I doubt it but better safe than sorry. I'm proud of what I've accomplished thus far. I've successfully uploaded my 1 minute plus video several times but it won't appear. Oh well. I'll try to load the videos tomorrow I guess. Just started raining. Just a light rain. Probably what fresh transplants like. 6/11 BEAUTIFUL outside. Today is the day. Most is done and I'll add the supports as trellis as they grow. This new grow space gets about twice as much sun as my old spot. The tar stops the burrowing insects. I'm getting everything out today. I just need to decide if I'm going to light dep that MASSIVE blueberry cheese or bite the bullet and put it in the fifty. Maybe I'll try to get some help from my cousin. Plants withstood the thunderstorm like Champs. I'll update as the day goes on. I planned better this year so I SHOULDN'T have an unmanageable jungle. UPDATE: WATERED THE 3 PLANTS LEFT IN THE 3 GALLONS (1 10th PLANET WITH GREAT BRANCH STRUCTURE AND TWO PURPLE PUNCH THAT WERE THE SMALLER ONES. IM GOING TO LIGHT DEP THEM. EVERYTHING IS OUTSIDE NOW IN A MIX OF 1/3 FOX FARM OCEAN FOREST, HAPPY FROG AND ROOTS ORGANIC 707 IN EQUAL PARTS. TRANSPLANTED WITH MYKOS. EVERYTHING IS OUTSIDE NOW. FINALLY DECIDED TO GET The MONSTER BLUE CHEESE I FIMed INTO ITS FOREVER HOME A 50 FALLON POT. IT WAS TGE EASIEST BEST TRANSPLANT OF THEM ALL. JUST LIKE AETTING A POT IN THE WHOLE. I DONT EVEN THINK THE PLANT NOTICED. ONE OF THE PURPLE PUNCHES HAD ROOTS OB ONE OF ITS LEAVES WHEN I LOOKED BUTVIT WAS STILL STANDING UO STEAIGHT. I KNOW SOME OF THE PLANTS LOOK DROOPY BUT WEVE HAD SEVERAL DAYS OF RAIN AND A MASDIVE THUNDER STORM. I BROUGHT OUT THE EARLIER TRANSPLANTS AS WELL. THEY ARE OUT OF THE SHOCK. WIND IS PRETTY HIGH BUT I DONT WANT TO ADD MORE TARPS. I THINK THINGS ARE SECURED GOOD ENOUGH FOR NOW. IM SUPER STOKED THAT TRANSPLANT WENT SO GOOD! Went back at 7:30pm. Some plants have a little bit of transplant shock and a little bit of burn but it's negligible considering they've been through a thunderstorm with pouring rain and extremely high winds. That blueberry cheese in the 50 transplanted so easy and still looks like it didn't even know the difference. I'll start light dep once I get a plan. Plants are recovering nicely but should something happen to one I could replace it. I'm interested in the light dep though and I've got a pretty good idea how to start. 6/12 The plants that had a little transplant shock are coming out of it. We had a thunderstorm and pouring rain the day of some of the transplants. Wasn't forecasted. High winds moved the plants around a bunch bit they are tough as hell. Everything is out now. Suppisedcto have showers the next few days. I'm putting together a feeding schedule abd setting up a spit to start light dep on the three plants I decided to flower early. The cage now gets twice as much sun as before. This is going to be a good year. Went back over and plants are perking up and doing even better everytime I see them. I took a video but it might not upload until tomorrow. I may start a new diary for the light dep. I have a few places I'm thinking to use for light dep. I need to make a solid plan before I start so maybe a day or two. Trying to upload again but I doubt it. 6/13 Had a rushed morning but I watered the plants in the 3 gallons as they were dry. I had added more soil to a couple of the bags. The top soil is startingvtj dry out but the plants look good and we are supposed to get rain so I don't really want to water if it's going to be raining. I think I've found my light dep spot and schedule. I'll update after I do something. WENT BACK OVER AND SPRAYED A LITTLE BIT OF WATER ON THE EXTREMELY DRY TOPSOIL THAT I HAD TOPPED SOME OF THE PLANTS OFF WITH. SHITTY THING IS THAT WAS ADDED AFTER THE THUNDER STORM SO IT MAKES IT HARDER TO JUSTE THE PLANTS WATERING NEEDS. I CAN TELL THAT ONE OFCTHE FIRST TEANSPLANTS IS STILL HOLDING WATER. I MEAN WE GOT A DOWNPOUR AND I DONT WABT TO WATER IF WE'RE GOING TO BE GETTING RAIN ANYWAY. IM GOING TO LOOK BACK OVER THE DIARY. THATS ONE GREAT THING ABOUT THESE. YOU CAN GO BACK AND CHECK YOUR WORK. 6/14 Glad I held off watering. It's raining today. Just showers. We haven't gotten any of that extreme wind we usually do. I have the plants supported but I still worry. Definitely more than I should. Plants are still doing good and acclimating to there homes. I have not started the light dep with the 3 gallons but I think I've found my spot. Once I start it will just be staying consistent putt8ng them in and out. I need to train them anyway. I have some research to do and a joint to smoke so I'll update later.
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All content on this diary is for inspirational and educational purposes only. The ideas shared are not a substitute for professional advice. This diary/account is not officially affiliated with Alan Watts or his estate. All materials are used under the principles of fair use. I honor the legacy of Alan Watts by sharing his wisdom respectfully and with the intention of inspiring awareness and self-understanding. 2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.
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It was finally time to harvest her, what good times we had with her. Amazed with how beautiful this plant is, how easy to manage and train and how rewarding Orange Hill Special is. So we said our thanks and goodbye, thanked her for all the joy she gave while growing her. I realize more and more how I love growing them even more than the end-product. It takes my mind of all going on for a while, in my miniature jungle world in the attic. Im sure all of you just sometimes sit there and watch them, in wonder, dreaming away with a good buzz on, watching them wave in the wind of the fans. So with a heavy hart I apologize, play them their last music, sit with them and feel them and smell them, watch the glistening of the cristals. In the end we had 209 gram dried Orange Hill Special, I will know the total of the grow, in a few days, since the final big whopper still has to dry: my XtraKush, be sure to check the harvest of that one *in couple of days) and see a one-dollar seed outperform two 10 dollar seeds. I probably go over 500 grams for the total grow, which would be 1.6 grams per watt. Big success! Consider an SP-3000 for your next light, you won't be disappointed! Check www.mars-hydro.com for the latest discounts! Thank you @MarsHydroLED for letting us try out your light, it made this successful grow possible! Greetings and all the best! Thank you to our friends here, our followers and the whole Growing Diaries community for just being there. Big Hug, Sunshine and BioBuds
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Girls started growing so fast due to the Aloe Vera Juice I had to flop them into flower early. They are just reeking of 🍋 right now smells like I'm in a lemon orchard. I think they are about done the transition. Hope they don't stretch much more.
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9/3 Shook the plants off this morning and took a few pics and did a video. Let's see if it uploads. Plants are doing good. Toasted toffy is due for another plant doctor application tonight. It seems like I'm losing lots of leaves. Looking back on my other diaries I guess it's normal. BUT I could've had pest/pathogen problems then. I'm over thinking it. Toasted toffy only needs a few more weeks. It will definitely outvrun the disease. The nugs are super dense too. The plant in the 50 is loosing leaves faster than I'd like. It is also one of the furthest along. The big leaves that turn yellow look like it's a calcium/magnesium issue. The stalk and branches are all bright green and healthy looking. So are the flowers. It could be something below the soil. I couldve been overwatering but last time I waited until it drooped. It will be fine though. I'm happy with my results so far. Not my best or biggest year but I'm going to put in the work and Mae these plants produce the best quality cannabis that im able too. Also I think plant doctor may kill insects. K bicarb with dawn does too. I've found a few dead bugs like flies and pillars. Pillars actually seem to be gone. Not many moths either. Unless I see them I'm not going to treat them for it. This is that magical time where you get to watch the plant produce those beautiful flowers. Despite some small problems I'm very grateful for what I have. I've worked hard for it. 9/4 Watered everything but the sherb pie which was still heavy with leaves yellowing. It's tied for the furthest behind in flower with the seed in the 10. I treated the toasted toffy with 1/4 gallon (4tsp/gallon) of plant doctor. I avoided flowers as much as possible and tried to just get a few leaves. I used the rest on the soil. I just wanted it to absorb a little faster. That's why I sprayed the foliage. It's not 4 days it's 14 days when tje plant doctor just leaves behind a bunch of potassium and phosphorus after it's done its job. Can't be used in a 14 day interval with copper and I think that's what treats septoria best. I dontvknow if I can use that in flower. Doesn't really matter. Things are looking good. Plant Doctor and the K bicarb seem to be doing there job effectively as long as i stick to the application times. I made a short video but didn't upload. I'll try again but I may have some medical emergencies that need to be dealt with. If not I should defoliate and tie down some event horizon branches. I thought about treating the other event horizon as well. I'm not positive it has septoria but it wouldn't hurt anything to be practice. It AT LEAST has a couple suspucuous spots. I smelled a skunk as soon as I got out of my car. It can't get in my grow but I might put out traps to catch it. We'll see how it goes. Video seems to be uploading. It's at 31% so I'll wait for it. I think I've got like four or five weeks on my toasted toffy and one event horizon and the one in the 50 that I THINK is red runtz. That's good that I'll have different plants to harvest at different times. It's seeds from here on out though. It was hard to find info on some of the strains I'm growing but they all have ONE thing in common. They are EXOTIC and they are at least MODERATELY DIFFICULT to grow. AT LEAST lol. Last year I picked the right seefs and strains and it greatly helped. Still, if these are difficult strains to grow, then I'm doing a good job. EDIT: I WAS STONED BUT IM PRETTY SURE I TREATED THE PLANT IN THE 50 WITH PLANT DOCTOR. DIDNT SPRAY BUDS. TRIED TO GET THE LEAVES THEN USED THE REST ON THE SOIL. I REMEMBER BECAUSE A STREAM ACCIDENTLY HIT A COUPLE LEAVES ON THE HUGE PLANT IN THE 10. 9/5 Sherb Pie was light and drooping. It's not to far in flower and still seemed moist yesterday with a little bit of weight. I watered it a gallon. I defoliated a little but I need to go through that plant. I have a bunch of plants that need a little work. The 50 gal seed is losing lots of leaves. I must not be feeding it enough, overwatering or maybe theres some sort of lock out. The plant looks nice and healthy. Leaves just turn yellow and die. Looked like a calcium or magnesium deficiency but i saw a septoria spot. I wouldn't think I've over feed or locked out using the small amounts of nutes I've used. It will be time to feed again soon. It's in the middle of flowering and still has leaves. I'm hoping things work out. I just have an anxiety disorder and worry too much. I'm GOING To have a great harve! I just need to keep moving forward and putting in the work. 9/6 What a fucking shitty morning. I did research on how to keep skunks out of your garden. Because I smelled it and the neighbors shot a few. Dad put traps out and it caught one. It wasn't a no spray trap so I approached it with him. Both had tarps. I kept telling him to throw it while I was distracting it bur he got a cramp and only got it over half. The half away from my garden. It's pretty far and down in a field but still. We had talked all morning about how to release it. I offered to drive their dog to take it for a walk so his alcoholic girlfriend wouldn't spiteful put the dog on its run. It wouldn't be sprayed but I made it out like it would ruin my plants. Picked dad up and got him to the bathroom. In that 30 seconds the stupid bitch grabbed the dog and brought it outside despite me YELLING "Can't you wait 5 minutes!? We've been dealing with this all morning. I heard fuck you in my head and she did it anyway. Dog ran at the skunk barking and it sprayed. Not a lot but I was fucking pissed. She's a full blown alcoholic and doesn't like me around early or the fact that my old man's 80 and house is mine. Anyway I got back and there was NO SKUNK SMELL. AT ALL! I WATERED EVERYTHING BUT SHERB PIE AND FED EVERYTHING BUT SHERB PIE. ILL FEED AND WATER THAT TOMORROW. WATERED A GALLON WITH A QUART OF FEED. Researched my strains found out they were all "difficult" to grow. One year ago to the Day I cut my first tenth planet. I got a couple close to a few weeks but the others will be later. If I can pull this off though I'll have some crazy quality! Grateful for what I have that's for sure! I doubled beastie bloom this time. We'll see how it goes.nothings uploading. I did some vids a pictures but I'll have to upload today. 9/7 I think it rained last night. I forgot to water and feed the sherb pie but I'll do it when I get back. I shook off the plants the best I could. I need to get the leaf blower out now and put it to use. Buds are swelling like crazy. GMOs are very tight flowers. Same with the event horizon. Rock hard nugs. With the gmo's I'm not worried. The were monster cropped so they have enough leaves even if some do die off. The toasted toffy only has like four weeks left. Same with the event horizon. The plant in the ten is the furthest behind but it's extremely healthy. I removed some leaves from the bottom and large fan leaves. The plant in the 50 I hope finishes before it loses all its leaves. I treated it with plant doctor and SOME people strip their plants but I don't. I'm grateful for what I've got. Won't be the biggest harvest but WHEN I make it to the finish line I will have some incredibly potent strains. I'm proud of myself to even get these to finish seeingvthe level of difficulty on cultivation. I'm trying to upload some stuff. We'll see. 9/8 Apparently it rained pretty hard last night. Took forever to go through and shake everything off and defoliate what needs it. Sherb pie is praying to the sky and almost totally purple. It's flowering so much more in the last couple days. Everything has. Even the one in the ten is stacking up! I defoliated most of what needed it and I was soaked. I noticed a branch on the middle gmo that was getting heavy and starting to break away from the trunk. It wad wobbly but I don't think there was a break un the Cambrian. I made a sling with rope (couldn't find zip ties) and I pulled it tight amd secured that branch to the tomato cage. GMO buds are hard as rocks. Everything is in overdrive. They liked that beastie bloom. I have cha ching but nothings ready for that yet I don't think. We'll the event horizon and toasted toffy might be I'll have to check. It's been beautiful today but not too hot. Lots of wind which is good. I'll upgrade supports as needed. Flowers are progressing at an amazing rate. Weather is supposed to be great the next few days. I'll keep this updated. 9/9 Had to shake the plants off. WATERED EVERYTHING BUT THE SHERB PIE WHICH WAS REALLY HEAVY. It's also lost a lot of leaves and the majority of leaves have turned purple. Toasted toffy and event horizon and red runts are pretty far into flower. I tried to take some pictures. Even the ten is blowing up! That looks like I'm going to switch to cha ching when flower gets further. Trichs are developing nicely on the ones further in flower. This year it's going to be quality over quantity. All these strains are ranked HARD to grow but have insane cannabinoid profiles and high THC %'s. So if I can keep it together for another month I'll be golden. Looking forward to it. This is the fun part. It's also the anxiety provoking part. I've learned a lot just since joining grow diaries.
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Well moving as planned kept her in only 6hr of direct sun rest of day in shade tgen night.. And sge flowered.. In middle of summer full sun cycle and im still able to prove WITHOUT DIRECT sun/light cannabis will flower ( photoperiods ) ..i cant afford her to get too tall... She grows very fast topped twice now sge has branches on top of brancges.. Only thing to do now is take some of her leafs but not now.. Love this genetics.. Very stable grows with no issues none...perfect thru her stages . now the stacking of flowers..
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Entering the final stage! 😋 Booth turn out compact but the buds are swelling nicelly and getting covered! 😍 Still some time to go so they´ll be harvest last... but really stocked for these! 😎
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@XanHalen
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Apr 09: Things are chuggin along, i think light may have been a factor in the brown spots, seeing similar symptoms on one of the other plants but very very minor in comparison.... I think it was too little calmag, i may have locked them out mid veg by using high concentration of nutes without watering till runoff (i now know, that is a no-go), and light stress. i have had them under 850-1050 ppfd when flipped to flower... one of them is loving the light, one is big and bushy (had to defoliate later than i wanted), and one has good bud structure but pistils seem thin, and growth seems slower (the one with the brown polkadot party on its body) so thinking it may be stunted. either way, im seeing denser buds, and im learning the ropes, thats enough for me :) Apr 12: Last feed day... Trichomes look just about ready, will do 2 runoff waterings 48hrs apart, then 24-36 hr darkness... Will try to post trichome pics.... Super excited to see the improvements from this run vs last run...!
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Se acercan las 2 ultimas semanas. Esta semana y luego se viene flawless. Tratando de bajar de a poco la EC y bajando la humedad para evitar problemas fungicos. Un olor fuerte a frutas tropicales, y mucha resina.
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-I am Back soon- Hope youre doing Great all 🙏 See ya -GGD
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Flowering day 15 since time change to 12 / 12 h Hey guys :-) This week the ladies developed beautifully 👍. The stretch has started very strongly :-) . Watering was done twice this week with 1.2 l (see table above for nutrients). The heating mat does exactly what it is supposed to do, you can see that the ladies are doing perfectly again 😃. Fresh osmosis water was mixed with tap water in a 100 liter tank so that I would have enough stale water for the coming week 👍. Otherwise everything was cleaned and checked. During the check, I noticed that after spraying neem oil 3 times, there were still a few damn trips to see. I have ordered nematodes for leaf and substrate against tripse. Then they should finally be gone again 🙏🏻 have fun and stay healthy 💚 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : Clearwater Seeds Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany
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