4. Woche Alle Ladys wurden 3x getoppt, jetzt mache ich eigentlich nicht mehr viel, nur noch bisschen in Form bringen und dann dürfen die Ladys wachsen, wie sie wollen 😁

Another good week in the garden. These girls are finishing up now. Some will be picked over the next few days and some will go another week here. Got some recharge and mammoth P for the first time three days ago. Hoping to finish strong 💪

She is growing strong and healthy...

So at the bottom tent we have amnesia haze who is very frosty with a sweet gassy smell. Then there is bubble gum more on the earthy side. At the top tent we have that GMO Cookies I got from the bottom of a bag is extremely frosty out of the three in there

We about a week in nd they are looking good, lots of growth this last week showing bright green leaves, im pretty sure shes done her final push and starting to put some energy into bud development!

She is by far the strongest strain I have ever grown flower sites everywhere

Hello the greater community that is GROWDIARIES!!! This week has been well, uplifting should I say! Two weeks ago I was pretty dismal, I had one out if three auto flowers. I had high hope for this grow with RQS being g pretty reputable, but all was going wrong! Then! with a little help from my friends here on GrowDiaries, I was inspired to carry on lol! Now a fortnight later, I'm my opinion, things have turned a corner! I'm seeing flowering behaviour again from these three that were completely stuck in veg! So I'm happy :) Supercropped the big sativa. Just made sense. Only one hasn't been HST, the runt who is actually most progressed of the three. Followed by big sativa. Feeds are pretty unique for each plant so just ask, but the one shown relates to the one in flower. Summary: 1 auto 3 photo periods all under 12/12 for the past 2 weeks. Thanks for taking a nosey!

Seeds were left in water overnight to simulate an all night soaking rain. The next day they were direct sown .75" deep into moist soil. They were left outside and because of the 40° nights took 4 days to pop. The soil was premixed with 15ml of rock dust and top dressed with the above listed nutrient at the listed ml rate per gallon of soil. 35ml of coop poop and 21ml of blood meal were mixed together and top dressed in a ring around plants, when they popped.

Week 3 is done and this girl had an interesting week. I noticed her yellowing tips on day 17 but only on the older growth? I'm stumped, surely it isn't the light intensity, still only at 450ish umols. The pot is always weighty, could it be an over water? Her sisters don't seem phased at all. As for environment, today I've turned the ventilation fan on 24hrs with no breaks. I started getting paranoid that hour on hour off is not doing them any favors and I'm just gonna have to suck it up and bare the brunt of the power bill. This will also drastically reduce the RH, which I'm not totally sure of the effects it will have on the girls. Low RH has got to be better than RH too high, right? 🤷‍♂️

Another good week with more growth, but not as big as I would like, so I moved the lights up higher halfway through the week. I also moved the fan onto a corner post and got bigger pots that I will transplant into this week. After topping last week, you can see new main stems starting. I also found out they need to be fed more than every other day as on friday morning a couple were a bit droopy. After feeding, later in the day, they had perked back up. I would like to give a shout out to Weed in a Pot for all the help I have gotten watching their videos and to let you know since their youtube channel is offline, they can be reached at http://www.weedinapot.com to see all their informative videos "so you, can know this too".

Las altas temperaturas han persistido es por esto que el riego ha sido más seguido variando siempre entre fertilizante y agua. Esta vez ya se utilizó Flawless Finish para este último proceso, una vez secándose el sustrato se comienza con la cosecha. Ha sido un buen cultivo. Veremos como termina esto. Seguimos ! 👽💪

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

3 seeds from barneys farm : glue gelato auto. Diary : Day 1: I dug a pit and cleaned my dirt with metal grid and a wheelbarrow & Installed the greenhouse. Day 2 : I rotated my dirt and added universal soil. 4 x 70l to area of 6m2. I also planted seeds in universal soil without sprouting first. Day 3 : I gave water and checked for sprouts. Bought moist meter and dripping hose. Installed those and making the greenhouse more moisty now. Feeling : Verry happy for my first time. Lots to learn.. . Excited

Fiquei fan desta semente é suave mas ao mesmo tempo pesada 😅😋

Heading into week 4 of Flower everything is budin up really nice Zake is the frostiest and really has a mint smell to it Looks like i already can say that breakpad breath will take more than 8 weeks Pink runtz x Hollywood has a Creazy Sweet smell to it

A good week in the bag. The girls seem to be doing well this round. They look healthy. They are getting water twice a week at this point. Everything's on track. There is one girl that has three leaves per node. Can't remember what that's called but it'll be fun to grow out

Still vegging looking like big bad ass bitches love it went organic a wee more water less nutrients was also thinking ov starting to ph my water m still grow in soil or well bio bizz all mix am loyal to the soil 😆

Start of week 4 I removed the net because of a heatwave a put the plants in a cold room during the night The coockies are now starting week 3 of flower i gave basic nutes everytime they were dry Day 24: the temperatures are perfect (24 celcius) the buds start to show up on all plants

I'm so sad just because I cannot get a lot of jars full of this wonderful haze man! Damn I love her aroma to pieces her terps are just awesome suuper sweet but you still can feel the sativa haze smell,but it's got a lot of berry and fruity smell tho,thank god I have this wonderful strain in Photoperiod version and I'll be able to grow her,definitely love this strain,I'll add her to my Photoperiod abd automatics list,AWESOME STRAIN! can't wait to smoke this,100% organic pure and clean free of any chemical sh%t thank you guys hope you like my work 💚✌️🌱💛❤️