Removed a lot of leaves...left top. Raised humidity for 5 days to 80 percent and so2

Week5 The week is over and all looks fine. Both ladies are preparing to bloom and are growing. Orange Sherbet is now 28 cm tall. Bruce Banner has caught up a lot this week and is at 25 cm. For 1 liter of water there was 0.5 ml of silicate;; 0.5 ml of Calmag;; 2 ml of BioNova BN-Zym, 2 ml of BioNova TheMissingLink;; 2 ml of BioNova Auto complete fertilizer and 1 ml of 100% organic algae juice. Ph 6.0-6.3 23°C

I removed the yellow fan leaves and some fell off on their own.

Finishing up from the looks of the trichomes and the breeders recommended time (+/- 56 days) they will be coming down soon! Still supplementing 2 hrs of UVB mid day, stopped nutrients and began flushing with flawless finish. They are looking so delicious and smelling very sweet and tropical, its scent radiates everywhere in the house from the basement to the second floor!! Also I recently remembered I never put on the carbon filter back since last grow smfh which is why it smells so strong outside.

She sure is turning into a pretty plant. She’s looking good!

Germination date 🌱 12/07/2021 Day 8 22/07/2021 Strain 🍁 Humboldt Purple Matcha (2nd time (Sherbinski x HSO) THC% • Unknown 💡 Mars Hydro FC4800 • Power draw 480W + 5% • Max coverage 5 x 5 • LED 2070pcsSamsungLM30B1&Osram660nm • Max Yield 2.5g / watt • Noise level 0 DB • Removable Driver +2m cable • Daisy chain (multiple lights) https://marshydroled.co.uk/products/mars-hydro-fc-4800-led-grow-light-samsunglm301b-commercial-greenhouse-medical-indoor-kit 🇬🇧 https://www.mars-hydro.com/buy-fc-4800-480w-4x4-energy-saving-full-spectrum-commercial-led-grow-light-mars-hydro-for-sale 🇺🇸 PROMO CODE • (ORG420) DISCOUNT 👍🏻 marshydroled.com ⛺ Mars Hydro 120 x 120 x 200cm 📤📥 AC infinity 6inch 💧 10lt dehumidifier ❄️ 3.1kw air con system 💉 Nutrients GreenBuzzLiquids Organic Grow Liquid • 1-4ml until 2wk flower Organic Bloom Liquid • 2-4ml flower stage Organic More PK • 2-4ml +wk3 of flower Organic Calmag • 1-2ml/lt whole grow Fast Plants Spray • first 3days at night lights off More Roots • 2-5ml veg +2wks flower Fast Buds • 5ml +wk2 of veg until 1wk flower Humic Acid Plus • 2-5ml whole grow Growzyme • 2-5ml whole grow Big Fruits • 2-5ml flower stage Clean Fruits • 5ml flush 1wk Ph powder Root Gel Living Organics PROMO CODE • organicnature420 15% off ✌️🏼 https://greenbuzzliquids.com/ 🥥 Growing Media • Coco Coir Notes 📝 Easy first week Plants look good and so does the mrs 😆 .. even better now the ac is in so one complete grow room. Lights doing it's job and Mars have kindly given me a promo code so if you want a cheeky discount use code (ORG420) 👍🏻Lowered it down to 70cm and will keep it there to get some stretch. New Nutrients I haven't had a problem with, the fast plant Spray is decent and I'm loving the pH powder. Gives you extra gains aswell being Organic. Thanks for all the love likes and comments and will update in a couple of days Happy growing fam ❤️🌱🍁👍🏻

KICKASS AUTO by KANNABIA Week #6 May 7th-14th Week#1 Flower This week she had continued to stretch both vertically and horizontal her side branches all have multiple bud sites on them she's a really bushy plant looks like she's going to produce a nice harvest. She's looking 👍 good no issues being outside she's actually thriving ✨️ right now. Stay Growing!! Kannabia.com Kickass Auto

Que pasa familia, vamos a añadir la primera semana de floración de estas Apple Fritter de RoyalQueenSeeds, empezamos con la temperatura que la tenemos entre los 21/25 grados, la humedad está entorno al 50%, el ph está en 6.2 , el foco actualmente lo tengo encendido 12 horas y la potencia es al 75% de lo que da el foco. También añado la gama de nutrientes de Agrobeta, que no falla, sin duda de la mejor alimentación que hay para tus plantas. Trasplantamos a macetas definitivas, 7 litros en este caso, también se ven bien verdes ya recuperaron la carencia de nitrógeno. Veremos estas próximas semanas como avanzan y se comportan en la floración. Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨💨

10-8-2024 TOP DRESSING AND WORM TEA..... NOTHING MORE 10-9-2024 22ND DAYS INTO FLOWERING SMELLS CRAZY ON THESE STRAIN GETTING SOME SNOW ON TOP LET'S GO 10-11-2024 DO NOT HAVE ANY PEST BUT JUST IN CASE NATURESGOODGUYS HOOK ME UP WITH LADYBUGS JUST GOT HANGING PATCH ABOUT /WITH SHIPPING 10-14-24 NOTHING TO DO...JUST WATER (PH6.6)AND WATCHING THE PLANTS....... COPY CAT GENETIX STRAINS ARE CRAZY!!!!!!!!

4 weeks into my first grow. What do you guys think?

Hello,, fin de semaines 11, floraison 5. Je dirais que dans l’ensemble tous ce passe correctement,, les buds se développent, elles commencent à prendre en volume. Et de plus en plus de cristaux apparaît aussi. L’odeur est aussi bien plus présente, j’ai donc mît mon filtre à charbon. La gorilla Cbd me semble très prometeuse, elle est très collante déjà avec une odeur délicieuse. La critical n’est pas en reste n’ont plus avec des note de parfum différent mais tout aussi délicieux,, 🤩 Pour l’arrosage cette semaine, en milieu, j’ai juste utilisé un peu de bio-bloom, histoire de pas trop les sur-fertiliser. Voilà voilà, c’est à peu près tout pour cette semain... Happy grow...😎

Veg week 2. Everything Looks good.

Still going like a tank. Slowed down slightly as i broke 2 branches training her but the branches have almost healed and shes bouncing back! Well happy with how its going, going to give her 2 more weeks veg. Any tips on getting her out of the 15l pot into the 20l flower pot?? Ive never transplanted anything this big cheers folks

Smell is very sweet.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

First Week Of Flowering Switch To 12/12 Went Good Room Checked For Light Leaks And Ducting Leaks All Seems Ok. Will Stake In A Few Weeks When Stretch Has Finished.

Being Mephisto Freebies there’s not a lot of info on how these particular seeds grow. I found them low maintenance, they weren’t the thirstiest girls I’ve grown and I found quite late on they didn’t like high temperatures, caused over half of them to foxtail. That being said the trichome production was there from week 2 of flower and by the time I cut them down they were some of the greasiest plants I’ve grown to date! I left a decent amount of the sugar leaves on while trimming with the hopes it will slow the drying process by a few extra days, between 7-10 days hanging, then into jars with humidity packs to cure. It’s been a fun grow and an exciting introduction into the Mephisto brand, over the last year I’ve managed to purchase a few different strains on there Monday drops so il definitely be running some more in the future. Hope this diary has been helpful ✌️ Il upload the dry weight before I put it all into jars.