Due to construction and revamping of our space, we had to leave all seedlings in their germination dome for approximately a month before getting them into veg. In order to not run into any space issues within the small dome, these young plants were kept under a low-wattage fluorescent tube to avoid any growth which may have been "too vigorous." VEG WEEK 1: 03/09/2021: (Image 1) Each plant was potted into, and fed with the following nutrients during transplant: 1. 5l FF F1 medium 2. GHF: Biogrow - 15g worked into medium 3. Wormcastings (workd into medium and astop dress) 4. Organics Matter Mycoroot Supreme -placed at the bottom of the planting hole. 5. Watered each plant with a Silicon Plus feed solution . 07/09/2021: (Image 2) - All plants are now in 5l pos with Freedom Farms F1 medium, and are in their new veg home under a 3500K 320W Quantum Board. - The 3 weakest seedlings of the total 19 seeds (1x Crit and 2x PC) were placed on the left side of the tent, and in different pots, as they are obviously struggling and may not even make it into our final flower room.

Uma planta que ainda está para ser testada em questão de uso, no planteio foi meia fraquinha mas devido ás circunstancias. Semente na terra no dia 17-04-2024 e cortada no dia 07-07-2024, 81 dias de vida!!!!!

Day8 im give for girls 1ml/l voodoo juice m, never before im don’t used this brand :d Day10 girls growing well i think i stard feeding for them Day14 i was have a small problem with ph bit im allredy buy new ph meter... wtf old one show 6.5 but real ph was 5,4 :( but this problem is eliminated! See you guys at week3!!

de muy baja produccion , pero ala vez de muy alta calidad

Started the flush 4 days ago. The main cola on this beast is the size of a 2 litre soda bottle. If I don't get 150g+ of this plant I'll be amazed.

Blütewoche 7: Nachdem ich den Großteil meiner Frustration über den Fehlschlag verarbeitet hatte, entschied ich mich dazu den Plan doch nochmal zu überdenken und separierte die gezwitterte Übeltäterin ins leere Nebenzelt um diese schonmal auf die letzte Reise zu schicken. Wie man auf den Bildern erkennen kann kontrollierte ich die Pflanzen an Tag 100 noch einmal genaustens auf Pollensäcke. Diese ließen auch nicht all zu lange auf sich warten und es waren doch mehr als gedacht. Aber als ich mir die Terpene anschaute und feststellte, dass der Großteil schon milchig ist und die Pflanze im Vergleich zur ChocolateHaze beispielsweise von Samen wirklich verschont ausschaut, stellte ich mir folgende Frage.. Ist es unter diesen Umständen nicht sinnvoller , die Pflanze zu nehmen wie sie ist und dafür das Risiko zu minimiere nur noch mehr Samen zu züchten? Natürlich würde ich sie lieber noch 2 Wochen in Blüte lassen, aber wer weiß wie viel Müsli in 2 Wochen nachkommen können.. Ich will es nicht herausfinden 😒😅 Also bereitete ich einen letztzen Trank zu, welcher der Pflanze für die kommenden Tage etwas " beruhigen " sollte. - To be Continued -

🌺🚀🌺🚀🌺🚀

First week of flower just lollipoped, minor pruning, selective defoliation. Changed tea brew for flowering, top dressed w/ local raw worm castings

Start flush

Nutrients are the same as the week before, as they seem to be happy and healthy, changing out the res didn’t seem like an efficient use of nutrients. I have added 15ml of tea and 10ml of CaMg+ into the 55l res over this past week. As I could see the slightest touch of chlorosis on the biggest girl of the bunch. This has naturally raised my EC from 0.72 to 0.89. Both plants either side of centre were topped on D25. They were both working on their sixth node at the time and I cut them both down to their third node, but just below the forth (so it has a stump). Middle will probs be topped in a couple of days time, it still has some catching to do. I will be changing the res out on D27, but I will post that nutrient mix on next weeks journal.

Smell starting to kick in 😁Looks like we have Pineapple pheno on our hands here! 😎 Increase feed on Boost and base nutrients, tho EC was one decimal less than last week due to lower tap water of EC 0.4 (previous week 0.5). Conducted major and final defoliation at the end of this week, arms and hands were so sticky had to wash them with meth. Vertical height starting to take space with even nug formation straight up (Stretch Rate: 1 - 2 Inches per day).

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

#myseed.ge #soil-N1 #mysun.ge #GROWROOM #MYSEED-GROWROOM We have very progressive vegetation in first 6 days... i am satisfied at all

Such a beautiful canopy! These ladies are all flowers now. Working on fattening up their rows of colas. And I mean ROWS! Healthy and strong and looking sexy. Definitely attributes that keep me happy. So easy going.... I can't wait to find out how generous they will be. They got treated with Dr zhymes as a preventative. That will basically wrap up their treatments ...possibly once more with lost coast. Upped the pk intake as they are blooming strong now. Gotta keep those engines well fuelled. Response was stellar. Happy happy happy ladies. Until next update. Happy growing and stay lit fam.

Update week 9 from seed bank @ColombiaGenomics

Still chugging along even though I've neglected them a little. A fun little side experiment, I learned a lot. At least I'll have flower from 6 more girls, not a complete waste.

Almost Done. 💚💚💚 From now on the ladies just gettin' tab water. Wedding Cake and Cookies Kush #2 r ready to go in a few days. Cookies Kush #1is lookin'like she needs at least 1 maybe 2 weeks from now 😂.

Just got done with trimming the 4x4 @greenhouseseedco Super Silver Haze / @blimburnseeds Silver Surfer Haze tent. The final yield is exactly 1000g plus some (kind of a lot)that I smoke in the last 2 weeks 😃 Also got 6, gallon size ziploc bags filled with good quality trim for concentrate. To be honest I was expecting a little more but keeping in mind that this grow I did not supplemented with bottled CO2 I think I’m good with the results. Definitely I enjoyed a lot this grow and I’ve learned even more.