All harvested. Results and pictures will come later

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

It's week 01 Day 02 Of 12/12 For My 02 Kombucha Cream By atlasseed . And For My Snow White & SpliffStrawberry By Spliff Seeds Amsterdam . So Yesterday All 4 Lady's Received there Transition Feed. And Prep For Flower. The Kombucha Cream 3-Part And Snow White. Received 4ml Of Emerald Harvest Nutrients Grow, Micro,Bloom,6ml of Emerald Goddess, 4ml Of King Kola, 4ml of Honey Chome And 4ml of Quad.AG Products Fulonic. pH at 6.3 ppm at 766. On Runoff. My Kombucha Cream 3-Part as a pH of 6.5 and ppm at 932. My Snow White as a pH of 6.4 and ppm is at 723. The Kombucha 2-Part and SpliffStrawberry Received 5ml of Emerald Harvest Cali Pro Grow A&B,6ml Emerald Goddess, 4ml King Kola,4ml Honey Chome And 4ml Of Quad.AG Fulonic. I pH the Kombucha Cream 2-Part at 6.2 Ppm at 613. On Runoff pH is at 6.3 ppm is at 833. My SpliffStrawberry I pH at 6.5 the pH was a little low on my last feed. Ppm is at 613 l. On Runoff pH is at 6.0 ppm is at 756. I like a pH of 6.2 in Flower I will work on getting the pH at 6.2 on the next few feeds.The Plants are really Loving Life Very happy and Perky This Morning. Happy Growing Growmies 🤘🏻

Hola de nuevo familia, dejamos la última semana de playa power plant xL. Aquí si vemos cómo la carencia de Nitrógeno afectó las hojas bajeras, pero nada serio. Solución: alimentar más . Lo demás lo podéis observar en las fotos y videos. todo va bien a excepción de una pequeña carencia .

Tropicana flowers 🌞☀️🌤️🌦️🌧️ Plant is 15 cm high because of low stress training. The length is 31 cm. Day 36: Sun is shining, 15-23 degrees, high humidity. Tropicana gets two liters of pure rainwater. Spend the day in my veggie garden working. In the evening I brought some 🐞for Tropicana because I spottet a few lice. My mood changes drastically as I find spider mites eggs, first and second stadium. Don‘t overthink that and decide to defoliate the plant immediately. I prepare a tee out of the leaves which brings fast relaxation. My mood is much better now. Day 37 In the morning with better light I find more spider mites and decide to wash the plant with rainwater. Can‘t find after any more of these little killer dwarfs. I decide to bring more beetles and check the leafs more often. Temperature is felling under 20 degrees, it‘s raining and the humidity is 87%. Day 38 In the early morning I couldn‘t find any mites on Tropicana. Got to continue controlling this in the next weeks regular. I‘m sure they are still there somewhere hidden in a dark spot. 🌦️ 13 - 21 degrees and humidity is around 60-70%. Day 39 🌦️🌧️ Very rainy day. Just let Tropicana enjoy life and tie down the top one more time. 🐞 stay since some days. One on every plant. I think the mites are a result of their start inside and no hygiene at all. Have the same with my peppers some years. 14 - 21 degrees, humidity around 84% Day 40 🌧️☁️🌤️☀️15 degrees, humidity 83% We listen to Roots Reggae, Tropicana is happy and quite busy with producing new roots, I guess. Don‘t understand when to stop low stress training. I think it’s enough now. She needs a rest. Day 41 🌧️⛈️🌧️🌧️ 15 - 19 degrees, humidity up to 89% We had rainfall today around 65 liters of water. I collected enough of it for the next weeks. Day 42 ☀️ 6 - 23 degrees. Humidity is falling. Tropicana enjoys the morning sun while she drinks and drinks and drinks…. Coffee. 😅

She grew well for the little time I spent with her. She grew fast as upping my room temps a few degrees sped the process up. She has a good smell and I will be back to update on a smoke report in a few months once it is dried, cured, and trimmed. Thank you, Medic Grow, and ILGM. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g.

New place outdoor. Bottom two branches and two leaves cut all tops too..

Last week went well. She has started showing signs of flowering and is showing some promise. She is drinking a decent amount and is starting to stretch I believe. I'm happy to see that I am going to get a decent turnout from her when I almost abandoned her in the beginning. I have used lst and supercropping to even out the canopy and it created what will be several tops. Plant took it well and handled being topped well. I think she will have a few decent colas if all goes well. Until next time happy growing to everyone!

Hey growers, welcome back to the Pineapple Upside Down grow journal — week 17 of veg. The plant keeps showing solid, consistent growth week after week. Her structure is getting stronger, and the canopy’s really starting to fill out nicely. Leaves are broad, green, and vibrant — a sign that she’s fully balanced and comfortable in her environment. Nutrient uptake looks perfect; the roots are clearly doing their job. I decided to raise the EC slightly, moving up to 1.8, since she’s been drinking more and showing signs of higher demand. pH remains stable, and the overall system is clean, well oxygenated, and running smoothly. For now, there’s no sign of deficiencies or stress. Everything’s running exactly as planned. I’m still unsure whether I’ll top her before moving to flower. That decision will depend on the final height and how much space she takes once she’s ready to transition into the flowering box. At this point, I prefer to let her develop naturally, focusing on strength and branch structure rather than manipulation. This phase is all about preparing her for a smooth transition into bloom, with maximum vigor and balanced nutrition. So for now, patience and observation. Next week should mark the last phase before flowering, and from there, things are going to get a lot more interesting. See you next week, growers — keep it clean, keep it green, and stay consistent. Peace

Seeing how the results are, the big ones I made apical and fmi species or the one you cut 3/4 I think they came out in a few days, some just cut the leaf and sprouted, I helped the biggest one with its final production and all of them general the different techniques, although curious results for a beginner, the techniques were not done well, they were cut halfway or only the leaves and not the apex itself ahahahaha, it turned out but very good in the end I would say

We have our color. Now for the buds to become more dense 😁 Edit: Just found out that this is a cross between Queen of the South from Relentless Genetics and Sugar Cane from Inhouse Genetics 🤯🤯🤯🤯

Starting low-stress training of the two main branches after 1st topping.

Ciao a tutti Dopo la bruttissima esperienza con la prima coltivazione sto provando ad aggiungere altre 3 piante al mio growbox. Ho preparato 1 litro di acqua a ph 6 con 4ml di root boost dove ho messo in ammollo 3 cubi in lana roccia. Dopo circa 24h è spuntata la prima piantina, ora è sotto lampada da circa 24h (speriamo bene), le altre due ancora non si sono fatte vedere.. _____________ Google translate _____________ Hello to all After the very bad experience with the first cultivation I am trying to add 3 more plants to my growbox. I prepared 1 liter of water at pH 6 with 4ml of root boost where I put 3 cubes in rock wool to soak. After about 24 hours the first map has been checked, now it is under a lamp for about 24 hours (hopefully good), the other two have not yet been seen .. _________________________ Set up - Box 1 x 1 x 1,8 mt - Sonlight Apollo PLUS Hyperled 10 (160x3w) 480W - Suolo Universal Cellmax - Fertilizzanti Cellmax

Week 13 (21-4 to 27-4) 21-4 Temps: 19.4 to 24.1 degrees Humidity: 54% to 63% Watering: 1000 ml. 1.6 6.2 22-4 Temps: 20.3 to 23.4 degrees Humidity: 51% to 65% Watering: 1000 ml. 0.4 6.2 23-4 Temps: 19.3 to 23.8 degrees Humidity: 50% to 64% Watering: 1000 ml. 1.6 6.2 Light set from 70% to 75% strength. 24-4 Temps: 19.4 to 23.4 degrees Humidity: 51% to 63% 25-4 Temps: 18 to 22.8 degrees Humidity: 52% to 61% Watering: 1000 ml. 0.4 6.2 26-4 Temps: 18.8 to 23.3 degrees Humidity: 49% to 61% Watering: 1000 ml. 1.6 6.2 27-4 Temps: 18.9 to 23.2 degrees Humidity: 47% to 59% Watering: 1000 ml. 0.4 6.2

Aaarg same problem😩 Screenshot🔝

Ist alles perfekt ph ec ist stabil

So things are going well can’t wait to get them in that system and the sensor push has been a great 50$ spent on it